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The optimal distance between two electrode tips during recording of compound nerve action potentials in the rat median nerve

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Neural Regeneration Research 2014年第2期9卷 171-178页

作者： Yongping Li Jie Lao Xin Zhao Dong Tian Yi Zhu xiaochun wei Department of Hand Surgery of HuaShan Hospital Fudan University Key Laboratory of Hand Reconstruction Ministry of Healthy Shanghai Key Laboratory of Peripheral Nerve and Microsurgery Department of Orthopedics the Second Hospital of Shanxi Medical University

The distance between the two electrode tips can greatly influence the parameters used for record- ing compound nerve action potentials. To investigate the optimal parameters for these recordings in the rat median nerve, we dissociated the nerve using different methods and compound nerve action potentials were orthodromically or antidromically recorded with different electrode spac- ings. Compound nerve action potentials could be consistently recorded using a method in which the middle part of the median nerve was intact, with both ends dissociated from the surrounding fascia and a ground wire inserted into the muscle close to the intact part. When the distance be- tween two stimulating electrode tips was increased, the threshold and supramaximal stimulating intensity of compound nerve action potentials were gradually decreased, but the amplitude was not changed significantly. When the distance between two recording electrode tips was increased, the amplitude was gradually increased, but the threshold and supramaximal stimulating intensity exhibited no significant change. Different distances between recording and stimulating sites did not produce significant effects on the aforementioned parameters. A distance of 5 mm between recording and stimulating electrodes and a distance of 10 mm between recording and stimulating sites were found to be optimal for compound nerve action potential recording in the rat median nerve. In addition, the orthodromic compound action potential, with a biphasic waveform that was more stable and displayed less interference （however also required a higher threshold and higher supramaximal stimulus）, was found to be superior to the antidromic compound action potential.

关键词： nerve regeneration peripheral nerve injury compound nerve action potential mediannerve electrodes amplitude supramaximal stimulus intensity recording electrode the Project 211in China neural regeneration

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Sr-Nd isotope stratification along water depth:An example from Datong hydrological station of Yangtze River

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Chinese Science Bulletin 2012年第34期57卷 4482-4490页

作者： LUO Chao ZHENG HongBo WU weiHua WANG Ping CHEN YuLiang wei xiaochun Key Laboratory of Surficial Geochemistry School of Earth Sciences and EngineeringNanjing UniversityNanjing 210093China School of Geography Nanjing Normal UniversityNanjing 210000China

This study examines the effects of hydrologic sorting and mixing of sources on the Sr-Nd isotopic compositions of suspended sediments at different water depths. The samples were collected from three layers (surface, middle and bottom) at Datong hydrological station of Yangtze River during the flood season of 2010. Our results show that, 87 Sr/ 86 Sr values decrease from surface to bottom, ranging from 0.730332 to 0.720857. ε Nd (0) values range from 14.75 to 10.09, with surface sediments being the most negative. The isotope composition at the middle layer can best represent the mean isotopic composition of the total suspended sediments transported by a river. It is believed that the stratification of Sr-Nd isotope is attributable to mixing of sediments from different sources due to hydrological sorting. Sediments from the upper stream are found to be coarser, and tend to contribute more to the lower water column. Although Sr-Nd isotope is a well acknowledged tool to trace sediment provenance, the current study suggests that the grain size of the samples and the sampling locations should be taken into consideration when applying this method to provenance study.

关键词： Sr-Nd同位素水文站长江水深悬浮沉积物同位素组成个例大通

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Carbon nanotubes-reinforced polylactic acid/hydroxyapatite porous scaffolds for bone tissue engineering

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Frontiers of Materials Science 2024年第1期18卷 59-69页

作者： weiwei Lan Mingbo Wang Zhenjun Lv Jun Li Fuying Chen Ziwei Liang Di Huang xiaochun wei weiyi Chen Research Center for Nano-Biomaterials&Regenerative Medicine Department of Biomedical EngineeringCollege of Biomedical EngineeringTaiyuan University of TechnologyTaiyuan 030024China Guangdong Engineering Technology Research Center of Implantable Medical Polymer Shenzhen Lando Biomaterials Co.Ltd.Shenzhen 518107China Shanxi-Zheda Institute of Advanced Materials and Chemical Engineering Taiyuan 030060China Department of Orthopaedics The Second Hospital of Shanxi Medical UniversityTaiyuan 030001China

In the field of bone defect repair,critical requirements for favorable cytocompatibility and optimal mechanical properties have propelled research efforts towards the development of composite *** this study,carbon nanotubes/polylactic acid/hydroxyapatite(CNTs/PLA/HA)scaffolds with different contents(0.5,1,1.5 and 2 wt.%)of CNTs were prepared by the thermally induced phase separation(TIPS)*** results revealed that the composite scaffolds had uniform pores with high porosities over 68%and high through *** addition of CNTs significantly enhanced the mechanical properties of resulted PLA/HA,in which the 1.5 wt.%CNTs/PLA/HA composite scaffold demonstrated the optimum mechanical behaviors with the bending elastic modulus of(868.5±12.34)MPa,the tensile elastic modulus of(209.51±12.73)MPa,and the tensile strength of(3.26±0.61)***,L929 cells on the 1.5 wt.%CNTs/PLA/HA scaffold displayed good spreading performance and favorable ***,it is expected that the 1.5 wt.%CNTs/PLA/HA scaffold has potential applications in bone tissue engineering.

关键词： CNTs/PLA/HA scaffold TIPS method mechanical property bone tissue engineering

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Mesenchymal stem cells loaded on 3D-printed gradient poly(e-caprolactone)/methacrylated alginate composite scaffolds for cartilage tissue engineering

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Regenerative Biomaterials 2021年第3期8卷 70-83页

作者： Yanyan Cao Peng Cheng Shengbo Sang Chuan Xiang Yang An xiaochun wei Zhizhong Shen Yixia Zhang Pengcui Li Key Lab of Advanced Transducers and Intelligent Control System of the Ministry of Education MicroNano System Research CenterCollege of Information and ComputerTaiyuan University of TechnologyTaiyuan 030024China College of Information Science and Engineering Hebei North UniversityZhangjiakou 075000China Shanxi Key Laboratory of Bone and Soft Tissue Injury Repair Department of OrthopedicsThe Second Hospital of Shanxi Medical UniversityTaiyuan 030001China Department of Plastic Surgery Peking University Third HospitalBeijing 100191China Department of Biomedical Engineering College of Biomedical EngineeringTaiyuan University of TechnologyTaiyuan 030024China

Cartilage has limited self-repair ability due to its avascular,alymphatic and aneural *** combination of three-dimensional(3D)printing and tissue engineering provides an up-and-coming approach to address this ***,we designed and fabricated a tri-layered(superficial layer(SL),middle layer(ML)and deep layer(DL))stratified scaffold,inspired by the architecture of collagen fibers in native cartilage *** scaffold was composed of 3D printed depth-dependent gradient poly(e-caprolactone)(PCL)impregnated with methacrylated alginate(ALMA),and its morphological analysis and mechanical properties were *** prove the feasibility of the composite scaffolds for cartilage regeneration,the viability,proliferation,collagen deposition and chondrogenic differentiation of embedded rat bone marrow mesenchymal stem cells(BMSCs)in the scaffolds were assessed by Live/dead assay,CCK-8,DNA content,cell morphology,immunofluorescence and real-time reverse transcription polymerase chain ***-loaded gradient PCL/ALMA scaffolds showed excellent cell survival,cell proliferation,cell morphology,collagen II deposition and hopeful chondrogenic differentiation compared with three individual-layer ***,our study demonstrates the potential use of the gradient PCL/ALMA construct for enhanced cartilage tissue engineering.

关键词： three-dimensional printing cartilage tissue engineering PCL methacrylated alginate bone marrow mesenchymal stem cells

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A fluid flow model in the lacunar-canalicular system under the pressure gradient and electrical field driven loads

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Applied Mathematics and Mechanics(English Edition) 2022年第6期43卷 899-916页

作者： Xiaogang WU Xiyu WANG Chaoxin LI Zhaowei WANG Yuqin SUN Yang YAN Yixian QIN Pengcui LI Yanqin WANG xiaochun wei weiyi CHEN College of Biomedical Engineering Taiyuan University of TechnologyTaiyuan 030024China Shanxi Provincial Key Laboratory for Repair of Bone and Soft Tissue Injury Taiyuan 030001China

The lacunar-canalicular system(LCS)is acknowledged to directly participate in bone tissue *** fluid flow in the LCS is synergic driven by the pressure gradient and electric field loads due to the electro-mechanical properties of *** this paper,an idealized annulus Maxwell fluid flow model in bone canaliculus is established,and the analytical solutions of the fluid velocity,the fluid shear stress,and the fluid flow rate are *** results of the fluid flow under pressure gradient driven(PGD),electric field driven(EFD),and pressure-electricity synergic driven(P-ESD)patterns are compared and *** effects of the diameter of canaliculi and osteocyte processes are *** results show that the P-ESD pattern can combine the regulatory advantages of single PGD and EFD patterns,and the osteocyte process surface can feel a relatively uniform shear stress *** the bone canalicular inner radius increases,the produced shear stress under the PGD or P-ESD pattern increases slightly but changes little under the EFD *** increase in the viscosity makes the flow slow down but does not affect the fluid shear stress(FSS)on the canalicular inner wall and osteocyte process *** increase in the high-valent ions does not affect the flow velocity and the flow rate,but the FSS on the canalicular inner wall and osteocyte process surface increases *** this study,the results show that the shear stress sensed by the osteocyte process under the P-ESD pattern can be regulated by changing the pressure gradient and the intensity of electric field,as well as the parameters of the annulus fluid and the canaliculus size,which is helpful for the osteocyte mechanical *** established model provides a basis for the study of the mechanisms of electro-mechanical signals stimulating bone tissue(cells)growth.

关键词： bone canaliculi osteocyte process pressure gradient electricity fluid shear stress(FSS)

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Effect of the disruption of three cytoskeleton components on chondrocyte metabolism in rabbit knee cartilage

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Chinese Medical Journal 2014年第21期127卷 3764-3770页

作者： Duan Wangping wei Lei Cao Xiaoming Guo Heng Wang Lei Hao Yongzhuang wei xiaochun Department of Orthopaedics Second Hospital of Shanxi Medical University Shanxi Key Lab of Bone and Soft Tissue Injury Repair Taiyuan Shanxi 030001 China Department of Orthopaedics The Warren Alpert Medical School of Brown University/Rhode Island Hospital Providence RI 02903 USA

Background Chondrocytes＇ phenotype and biosynthesis of matrix are dependent on having an intact cytoskeletal ***,microtubules,and intermediate filaments are three important components of the cytoskeletal structure of *** aims of this study were to determine and compare the effects of the disruption of these three cytoskeletal elements on the apoptosis and matrix synthesis by rabbit knee chondrocytes in *** Chondrocytes were isolated from full-thickness knee cartilage of two-month-old rabbits using enzymatic methods （n=24）.The isolated cells were stabilized for three days and then exposed to low,medium,and high doses of chemical agents that disrupt the three principal cytoskeletal elements of interest：colchicine for microtubules,acrylamide for intermediate filaments,and cytochalasin D for actin microfilaments.A group of control cells were treated with *** apoptosis was assessed using the Annexin-FITC binding assay by flow cytometry on days 1 and 2 after exposure to the disrupting chemical *** components and distribution of the cytoskeleton within the cells were analyzed by laser scanning confocal microscopy （LSCM） with immunofluorescence staining on day *** mRNA levels of aggrecan （AGG） and type Ⅱ collagen （Col-2） and their levels in culture medium were analyzed using real-time PCR and enzymelinked immunosorbent serologic assay （ELISA） on days 3,6,and *** In the initial drug-dose-response study,there was no significant difference in the vitality of cells treated with 0.1 μmol/L colchicine,2.5 mmol/L acrylamide,and 10 μg/L cytochalasin D for two days when compared with the control group of *** concentrations of colchicine and acrylamide treatment selected above significantly decreased the number of viable cells over the nine-day culture and disrupted significantly more cell ***-time PCR and ELISA results showed that the mRNA levels and medium concentrations of AGG and Col-2 were signifi

关键词： knee chondrocyte cytoskeleton aggrecan collagen apoptosis

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Compression regulates gene expression of chondrocytes through HDAC4 subcellular nuclear relocation via PP2A-depended HDAC4 dephosphorylation

Compression regulates gene expression of chondrocytes throug...

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第十一届全国生物力学学术会议暨第十三届全国生物流变学学术会议

作者： Chongwei Chen xiaochun wei Lei wei Department of Orthopaedics Second Hospital of Shanxi Medical UniversityShanxi Key Laboratory of Bone and Soft Tissue Injury Repair Department of Orthopaedics The Warren Alpert Medical School of Brown University/Rhode Island Hospital

Objective;The mechanisms that chondrocytes sense and respond to mechanical stimulation remain largely ***4 has a surprising ability to translocate between the cytoplasm and ***4 subcellular relocation plays a critical role in cell differentiation and *** it is unclear whether mechanical loading can inducd HDAC4 subcellular *** this study,we aim to investigate the effect of compression on

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CCS Technology Helpful for China to Improve Capability of Treaty Execution

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Bulletin of the Chinese Academy of Sciences 2009年第4期23卷 237-239页

作者： LI xiaochun wei Ning FANG Zhiming LI Qi HU Haixiang State key Laboratory of Geomechanics and Geotechnical Engineeringnstitution of Rock and Soil Mechanics Chinese Academy of Sciences Wuchang Wuhan 430071 China

The International Energy Agency (IEA) Report 2008 illustrated that the CO2 emissions from energy consumption in China was 5.6 billion tons in 2006,accounting for 20% of the global total (28 billion tons).

关键词：中国二氧化碳排放量 CCS 能力技术国际能源机构能源消费经济发展

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Anti-catabolic effect of caffeic acid phenethyl ester, an active component of honeybee propolis on bone loss in ovariectomized mice： a micro-computed tomography study and histological analysis

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Chinese Medical Journal 2014年第22期127卷 3932-3936页

作者： Duan Wangping Wang Qing Li Fang Xiang Chuan Zhou Lin Xu Jiake Feng Haotian wei xiaochun Department of Orthopaedics the Second Hospital of Shanxi Medical University Shanxi Key Lab of Bone and Soft Tissue Injury RepairTaiyuan Shanxi 030001 China Institute of Medical Information School of Biomedical EngineeringSouthern Medical University Guangzhou Guangdong 510515China School of Medicine and Pathology University of Western AustraliaPerth 6009 Australia Program of Nutrition and Bone & Joint Health Nestlé R&D(China)Ltd Beijing 100095 China

Background Osteoporosis （OP） is a common bone disease,which adversely affects life *** treatments are necessary to combat both the loss and fracture of *** studies indicated that caffeic acid phenethyl ester （CAPE） is a natural chemical compound from honeybee propolis which is capable of attenuating osteoclastogenesis and bone ***,this study aimed to investigate the effect of CAPE on bone loss in OP mice using micro-computed tomography （CT） and *** Eighteen mice were prepared and evenly divided into three *** six mice in the sham＋PBS group did not undergo ovariectomy and were intraperitoneally injected with PBS during the curing *** mice were ovariectomized （OVX） to induce *** of them in the OVX＋CAPE group were intraperitoneally injected with 0.5 mg/kg CAPE twice per week for 4 weeks after *** other six OVX mice in OVX＋PBS group were treated with *** the mice were sacrificed 4 weeks after *** tibias were bilaterally excised for micro-CT scan and histological *** Mann-Whitney U test was used to test the statistical differences among *** Bone loss occurred in OVX *** with the sham＋PBS group,mice in the OVX＋PBS group exhibited a significant decrease in bone mineral density （BMD,P ＜0.05）,bone volume fraction （BV/TV,P ＜0.01）,trabecular thickness （***,P ＜0.05）,and trabecular number （Tb.N,P ＜0.01）,as well as a non-insignificant increase in the number of osteoclasts （***/***）.With CAPE treatment,the microarchitecture of the tibial metaphyses was significantly improved with a reduction of osteoclast *** with the OVX＋PBS group,BV/TV in the OVX＋CAPE group was significantly increased by 33.9％（P ＜0.05）.Conclusion CAPE therapy results in the protection of bone loss induced by OVX.

关键词： caffeic acid phenethyl ester osteoporosis bone loss micro-computed tomography

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城市街道空间的非正规化演变——武汉市汉正街的个案(1988-2013年)

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城市规划 2013年第4期37卷 74-80页

作者：陈煊魏小春湖南大学城市规划系福州市规划设计研究院

本研究旨在反思城市更新的模式、参与的主体是否存在更加多元化的选择。研究截取了武汉市汉正街1988年以来街道空间的非正规化演变的空间实践片段,着重分析民众行为如何作用于城市街道,进而洞察与解释我国街道非正规演变的真实运行逻辑... 详细信息

本研究旨在反思城市更新的模式、参与的主体是否存在更加多元化的选择。研究截取了武汉市汉正街1988年以来街道空间的非正规化演变的空间实践片段,着重分析民众行为如何作用于城市街道,进而洞察与解释我国街道非正规演变的真实运行逻辑。研究认为,地方政府的强权力决策与弱治理绩效暗含了规划蓝图与民众现实生活需求的矛盾性等特征,地方政府、开发商、民众在城市更新过程中复杂的互动结构中处于博弈共生的关系。

关键词：街道城市更新非正规性建设自建

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