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CRISPR/CasRx-mediated resistance to Soybean mosaic virus in soybean

The Crop Journal 2024年第4期12卷 1093-1101页

作者： Le Gao Lijun Xie Yanmin xiao Xinge Cheng Ruosi Pu Ziheng Zhang Yu Liu Shaopei Gao Zilong Zhang Haoran qu Haijian Zhi Kai Li MARA Key Laboratory of Biology and Genetics Improvement of Soybean Zhongshan Biological Breeding Laboratory(ZSBBL)National Innovation Platform for Soybean Breeding and Industry-Education IntegrationState Key Laboratory of Crop Genetics&Germplasm Enhancement and UtilizationNanjing Agricultural UniversityNanjing 210095JiangsuChina Department of Horticulture Beijing Vocational College of AgricultureBeijing 102442China College of Agronomy&Biotechnology China Agricultural UniversityBeijing 100193China College of Bioscience and Biotechnology Shenyang Agricultural UniversityShenyang 110866LiaoningChina

Soybean mosaic virus(SMV),an RNA virus,is the most common and destructive pathogenic virus in soybean *** newly developed CRISPR/Cas immune system has provided a novel strategy for improving plant resistance to viruses;hence,this study aimed to engineer SMV resistance in soybean using this ***,multiple sgRNAs were designed to target positive-and/or negative-sense strands of the SMV HC-Pro ***,the corresponding CRISPR/CasRx vectors were constructed and transformed into *** inoculation with SMV,39.02%,35.77%,and 18.70%of T_(1)plants were confirmed to be highly resistant(HR),resistant(R),and mildly resistant(MR)to SMV,respectively,whereas only 6.50%were identified as susceptible(S).Additionally,qRT-PCR and DAS-ELISA showed that,both at 15 and 30 d post-inoculation(dpi),SMV accumulation significantly decreased or was even undetectable in HR and R plants,followed by MR and S ***,the expression level of the CasRx gene varied in almost all T_(1)plants with different resistance level,both at 15 and 30 ***,when SMV resistance was evaluated in the T_(2)generation,the results were similar to those recorded for the T_(1)*** findings provide new insights into the application of the CRISPR/CasRx system for soybean improvement and offer a promising alternative strategy for breeding for resistance to biotic stress that will contribute to the development of SMV-immune soybean germplasm to accelerate progress towards greater soybean crop productivity.

关键词： Soybean RNA virus Soybean mosaic virus Resistance CRISPR/CasRx

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Fenofibrate-promoted hepatomegaly and liver regeneration are PPARα-dependent and partially related to the YAP pathway

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Acta Pharmaceutica Sinica B 2024年第7期14卷 2992-3008页

作者： Shicheng Fan Yue Gao Pengfei Zhao Guomin Xie Yanying Zhou xiao Yang Xuan Li Shuaishuai Zhang Frank JGonzalez Aijuan qu Min Huangb Huichang Bi NMPA Key Laboratory for Research and Evaluation of Drug Metabolism&Guangdong Provincial Key Laboratory of New Drug Screening&Guangdong-Hongkong-Macao Joint Laboratory for New Drug Screening School of Pharmaceutical SciencesSouthern Medical UniversityGuangzhou 510515China Guangdong Provincial Key Laboratory of New Drug Design and Evaluation School of Pharmaceutical SciencesSun Yat-sen UniversityGuangzhou 510006China Department of Physiology and Pathophysiology School of Basic Medical SciencesCapital Medical UniversityBeijing 100069China Laboratory of Metabolism Center for Cancer ResearchNational Cancer InstituteNational Institutes of HealthBethesdaMD 20892USA The State Key Laboratory of Chemical Oncogenomics School of Chemical Biology and BiotechnologyShenzhen Graduate School of Peking UniversityShenzhen 518055China

Fenofibrate,a peroxisome proliferator-activated receptor α(PPARα)agonist,is widely prescribed for hyperlipidemia *** studies also showed that it has therapeutic potential in various liver ***,its effects on hepatomegaly and liver regeneration and the involved mechanisms remain ***,the study showed that fenofibrate significantly promoted liver enlargement and regeneration post-partial hepatectomy in mice,which was dependent on hepatocyteexpressed PPARα.Yes-associated protein(YAP)is pivotal in manipulating liver growth and *** further identified that fenofibrate activated YAP signaling by suppressing its K48-linked ubiquitination,promoting its K63-linked ubiquitination,and enhancing the interaction and transcriptional activity of the YAP-TEAD *** inhibition of YAP-TEAD interaction using verteporfin or suppression of YAP using AAV Yap shRNA in mice significantly attenuated fenofibrate-induced *** factors,such as MYC,KRT23,RAS,and RHOA,might also participate in fenofibrate-promoted hepatomegaly and liver *** studies demonstrate that fenofibratepromoted liver enlargement and regeneration are PPARα-dependent and partially through activating the YAP signaling,with clinical implications of fenofibrate as a novel therapeutic agent for promoting liver regeneration.

关键词： Fenofibrate PPARa Hepatomegaly Partial hepatectomy Liver regeneration YAP TEAD Ubiquitination

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Chondroitinase ABC combined with Schwann cell transplantation enhances restoration of neural connection and functional recovery following acute and chronic spinal cord injury

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Neural Regeneration Research 2025年第5期20卷 1467-1482页

作者： Wenrui qu Xiangbing Wu Wei Wu ying Wang Yan Sun Lingxiao Deng Melissa Walker Chen Chen Heqiao Dai Qi Han ying Ding Yongzhi Xia George Smith Rui Li Nai-Kui Liu xiao-Ming Xu Spinal Cord and Brain Injury Research Group Stark Neurosciences Research InstituteDepartment of Neurological SurgeryIndiana University School of MedicineIndianapolisINUSA Department of Hand Surgery the Second Hospital of Jilin UniversityChangchunJilin ProvinceChina Shriners Hospitals Pediatric Research Center Temple University School of MedicinePhiladelphiaPAUSA Department of Anatomy&Cell Biology Indiana University School of MedicineIndianapolisINUSA

Schwann cell transplantation is considered one of the most promising cell-based therapy to repair injured spinal cord due to its unique growth-promoting and myelin-forming properties.A the Food and Drug Administration-approved Phase I clinical trial has been conducted to evaluate the safety of transplanted human autologous Schwann cells to treat patients with spinal cord injury.A major challenge for Schwann cell transplantation is that grafted Schwann cells are confined within the lesion cavity,and they do not migrate into the host environment due to the inhibitory barrier formed by injury-induced glial scar,thus limiting axonal reentry into the host spinal *** we introduce a combinatorial strategy by suppressing the inhibitory extracellular environment with injection of lentivirus-mediated transfection of chondroitinase ABC gene at the rostral and caudal borders of the lesion site and simultaneously leveraging the repair capacity of transplanted Schwann cells in adult rats following a mid-thoracic contusive spinal cord *** report that when the glial scar was degraded by chondroitinase ABC at the rostral and caudal lesion borders,Schwann cells migrated for considerable distances in both rostral and caudal *** Schwann cell migration led to enhanced axonal regrowth,including the serotonergic and dopaminergic axons originating from supraspinal regions,and promoted recovery of locomotor and urinary bladder ***,the Schwann cell survival and axonal regrowth persisted up to 6 months after the injury,even when treatment was delayed for 3 months to mimic chronic spinal cord *** findings collectively show promising evidence for a combinatorial strategy with chondroitinase ABC and Schwann cells in promoting remodeling and recovery of function following spinal cord injury.

关键词： axonal regrowth bladder function chondroitinase ABC functional recovery glial scar lentivirus migration Schwann cell spinal cord injury transplantation

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A Lightweight Network with Dual Encoder and Cross Feature Fusion for Cement Pavement Crack Detection

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Computer Modeling in Engineering & Sciences 2024年第7期140卷 255-273页

作者： Zhong qu Guoqing Mu Bin Yuan School of Computer Science and Technology Chongqing University of Posts and TelecommunicationsChongqing400065China School of Software Engineering Chongqing University of Posts and TelecommunicationsChongqing400065China

Automatic crack detection of cement pavement chiefly benefits from the rapid development of deep learning,with convolutional neural networks(CNN)playing an important role in this ***,as the performance of crack detection in cement pavement improves,the depth and width of the network structure are significantly increased,which necessitates more computing power and storage *** limitation hampers the practical implementation of crack detection models on various platforms,particularly portable devices like small mobile *** solve these problems,we propose a dual-encoder-based network architecture that focuses on extracting more comprehensive fracture feature information and combines cross-fusion modules and coordinated attention mechanisms formore efficient feature ***,we use small channel convolution to construct shallow feature extractionmodule(SFEM)to extract low-level feature information of cracks in cement pavement images,in order to obtainmore information about cracks in the shallowfeatures of *** addition,we construct large kernel atrous convolution(LKAC)to enhance crack information,which incorporates coordination attention mechanism for non-crack information filtering,and large kernel atrous convolution with different cores,using different receptive fields to extract more detailed edge and context ***,the three-stage feature map outputs from the shallow feature extraction module is cross-fused with the two-stage feature map outputs from the large kernel atrous convolution module,and the shallow feature and detailed edge feature are fully fused to obtain the final crack prediction *** evaluate our method on three public crack datasets:DeepCrack,CFD,and *** results on theDeepCrack dataset demonstrate the effectiveness of our proposed method compared to state-of-the-art crack detection methods,which achieves Precision(P)87.2%,Recall(R)87.7%,and F-score(F1)87.4%.Thanks to our lightweight cr

关键词： Shallow feature extraction module large kernel atrous convolution dual encoder lightweight network crack detection

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An object detection approach with residual feature fusion and second-order term attention mechanism

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CAAI Transactions on Intelligence Technology 2024年第2期9卷 411-424页

作者： Cuijin Li Zhong qu Shengye Wang College of Computer Science and Technology Chongqing University of Posts and TelecommunicationsChongqingChina College of Electronic Information Chongqing Institute of EngineeringChongqingChina

Automatically detecting and locating remote occlusion small objects from the images of complex traffic environments is a valuable and challenging *** the boundary box location is not sufficiently accurate and it is difficult to distinguish overlapping and occluded objects,the authors propose a network model with a second-order term attention mechanism and occlusion ***,the backbone network is built on *** a method is designed for the feature extraction network based on an item-wise attention mechanism,which uses the filtered weighted feature vector to replace the original residual fusion and adds a second-order term to reduce the information loss in the process of fusion and accelerate the convergence of the ***,an objected occlusion regression loss function is studied to reduce the problems of missed detections caused by dense *** experimental results demonstrate that the authors’method achieved state-of-the-art performance without reducing the detection *** mAP@.5 of the method is 85.8%on the Foggy_cityscapes dataset and the mAP@.5 of the method is 97.8%on the KITTI dataset.

关键词： artificial intelligence computer vision image processing machine learning neural network object recognition

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Narrowband blue emitter based on fused nitrogen/carbonyl combination with external quantum efficiency approaching 30%

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Science China Chemistry 2024年第1期67卷 351-359页

作者： Long Chen Jia-Heng Cai You-Jun Yu Yang-Kun qu Sheng-Yi Yang Sheng-Nan Zou Rui-Hong Liu Dong-ying Zhou Liang-Sheng Liao Zuo-quan Jiang Institute of Functional Nano&Soft Materials(FUNSOM) Jiangsu Key Laboratory for Carbon-Based Functional Materials&DevicesSoochow UniversitySuzhou 215123China Macao Institute of Materials Science and Engineering Macao University of Science and TechnologyMacao 999078China

Multi-resonance thermally activated delayed fluorescence(MR-TADF)emitters can enable narrowband emission with high color purity and electroluminescence ***/carbonyl(N/C=O)system is receiving increasing attention while the nitrogen/boron(N/B)system has been widely *** decoration is an effective approach for N/B type MR-TADF system but always leads to broadening and red-shifting of the emission band in N/C=O MR-TADF *** attribute these unfavorable phenomena to the formation of intramolecular charge transfer between the MR-core and peripheral *** address this issue,we have developed a new strategy by decorating DMQAO(a fused N/C=O MR-core)with a triazine acceptor and a neutral terphenyl group to construct MTDMQAO and MBDMQAO,*** introduction of the triazine acceptor not only realizes efficient narrowband emission in MTDMQAO,but also accelerates the reverse intersystem crossing process through enhanced spin-orbital *** a result,MTDMQAO exhibits a significantly higher external quantum efficiency of 29.4%compared to the referent emitters,validating the rationality of our derivation *** study highlights the potential of the N/C=O system for MR-TADF emitters and provides important insights for understanding the difference between N/B and N/C=O systems.

关键词： carbonyl group narrowband acceptor decoration thermally activated delayed fluorescence OLEDs

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Information manifold and fault detection of multi-agent systems

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Chinese Journal of Aeronautics 2024年第10期37卷 410-423页

作者： Ruotong qu Bin JIANG Yuehua CHENG xiaodong HAN College of Automation Engineering Nanjing University of Aeronautics and AstronauticsNanjing 210016China China Academy of Space Technology Beijing 100098China

With the increase of the number of agents in multi-agent systems and the rapid increase of the complexity of the overall structure of the system,the fault detection and diagnosis work has brought great *** have carried out considerable research work on fault detection and diagnosis of multi-agent systems,but there is no research on fault state estimation and diagnosis based on the information and state of the whole multi-agent *** on the global perspective of information geometry theory,this paper presents two new physical quantities of the information manifold of multi-agent systems,as Lagrangian and energy–momentum tensor,to express the state of the overall information of multi-agent systems,and to characterize the energy state and development trend of *** this paper,two new physical parameters are introduced into the research of multi-agent fault detection and diagnosis,and the fault state and trend of multi-agent system are evaluated from the global perspective,which provides more comprehensive theoretical support for designing more scientific and reasonable fault diagnosis and fault recovery *** of the application example confirms the competitive performance of the proposed method.

关键词： Multi-Agent Systems(MASs) Fault information manifold Lagrangian Fault detection Energy-momentum Tensor

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Exploring non-invasive diagnostics for metabolic dysfunctionassociated fatty liver disease

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World Journal of Gastroenterology 2024年第28期30卷 3447-3451页

作者： Biao qu Zheng Li Department of Clinical Pharmacology The Second Hospital of Anhui Medical UniversityHefei 230601Anhui ProvinceChina Jiangsu Engineering Research Center of Cardiovascular Drugs Targeting Endothelial Cells College of Health SciencesSchool of Life SciencesJiangsu Normal UniversityXuzhou 221000Jiangsu ProvinceChina

The population with metabolic dysfunction-associated fatty liver disease(MAFLD)is increasingly common *** of people at risk of progression to advanced stages is necessary to timely offer interventions and appropriate *** biopsy is currently considered the gold standard for the diagnosis and staging of MAFLD,but it has associated risks and *** has spurred the exploration of non-invasive diagnostics for MAFLD,especially for steatohepatitis and *** non-invasive approaches mostly include biomarkers and algorithms derived from anthropometric measurements,serum tests,imaging or stool metagenome ***,they still need rigorous and widespread clinical validation for the diagnostic performance.

关键词： Metabolic dysfunction-associated fatty liver disease Non-invasive diagnostics Circulating biomarkers Imaging biomarkers Stool microbial biomarkers

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Activation of autophagy by Citri Reticulatae Semen extract ameliorates amyloid-beta-induced cell death and cognition deficits in Alzheimer’s disease

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Neural Regeneration Research 2024年第11期19卷 2467-2479页

作者： Yong Tang Jing Wei xiao-Fang Wang Tao Long xiaohong Xiang Liqun qu Xingxia Wang Chonglin Yu Xingli xiao Xueyuan Hu Jing Zeng Qin Xu Anguo Wu Jianming Wu Dalian Qin xiaogang Zhou Betty Yuen-Kwan Law Dr.Neher’s Biophysics Laboratory for Innovative Drug Discovery State Key Laboratory of Quality Research in Chinese MedicineMacao University of Science and TechnologyMacao Special Administrative RegionChina Sichuan Key Medical Laboratory of New Drug Discovery and Druggability Evaluation Luzhou Key Laboratory of Activity Screening and Druggability Evaluation for Chinese Materia Medica Southwest Medical UniversityLuzhouSichuan ProvinceChina Eye School and Key Laboratory of Sichuan Province Ophthalmopathy Prevention&Cure and Visual Function Protection of Chengdu University of TCM ChengduSichuan ProvinceChina School of Basic Medical Sciences Southwest Medical UniversityLuzhouSichuan ProvinceChina Department of Ophthalmology The Affiliated Hospital of Southwest Medical UniversityLuzhouSichuan ProvinceChina Department of Neurology The First People’s Hospital of NeijiangNeijiangSichuan ProvinceChina

Amyloid-beta-induced neuronal cell death contributes to cognitive decline in Alzheimer’s *** Reticulatae Semen has diverse beneficial effects on neurodegenerative diseases,including Parkinson’s and Huntington’s diseases,however,the effect of Citri Reticulatae Semen on Alzheimer’s disease remains *** the current study,the anti-apoptotic and autophagic roles of Citri Reticulatae Semen extract on amyloid-beta-induced apoptosis in PC12 cells were first *** Reticulatae Semen extract protected PC12 cells from amyloid-beta-induced apoptosis by attenuating the Bax/Bcl-2 ratio via activation of *** addition,Citri Reticulatae Semen extract was confirmed to bind amyloid-beta as revealed by biolayer interferometry in vitro,and suppress amyloid-beta-induced pathology such as paralysis,in a transgenic Caenorhabditis elegans in vivo ***,genetically defective Caenorhabditis elegans further confirmed that the neuroprotective effect of Citri Reticulatae Semen extract was *** importantly,Citri Reticulatae Semen extract was confirmed to improve cognitive impairment,neuronal injury and amyloid-beta burden in 3×Tg Alzheimer’s disease *** revealed by both in vitro and in vivo models,these results suggest that Citri Reticulatae Semen extract is a potential natural therapeutic agent for Alzheimer’s disease via its neuroprotective autophagic effects.

关键词： Alzheimer’s disease amyloid-beta apoptosis autophagy Caenorhabditis elegans Citri Reticulatae Semen

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Aging hallmarks of the primate ovary revealed by spatiotemporal transcriptomics

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Protein & Cell 2024年第5期15卷 364-384页

作者： Huifen Lu ying Jing Chen Zhang Shuai Ma Weiqi Zhang Daoyuan Huang Bin Zhang Yuesheng Zuo yingying Qin Guang-Hui Liu Yang Yu Jing qu Si Wang Advanced Innovation Center for Human Brain Protection National Clinical Research Center for Geriatric DisordersXuanwu Hospital Capital Medical UniversityBeijing 100053China State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China Aging Translational Medicine Center International Center for Aging and CancerBeijing Municipal Geriatric Medical Research CenterXuanwu HospitalCapital Medical UniversityBeijing 100053China State Key Laboratory of Membrane Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China Beijing Institute for Stem Cell and Regenerative Medicine Beijing 100101China CAS Key Laboratory of Genomic and Precision Medicine Beijing Institute of GenomicsChinese Academy of SciencesBeijing 100101China Institute for Stem cell and Regeneration CASBeijing 100101China China National Center for Bioinformation Beijing 100101China The Fifth People’s Hospital of Chongqing Chongqing 400062China Sino-Danish College University of Chinese Academy of SciencesBeijing 101408China Sino-Danish Center for Education and Research Beijing 101408China Department of Obstetrics and Gynecology Center for Reproductive MedicinePeking UniversityThird HospitalBeijing 100191China Clinical Stem Cell Research Center Peking UniversityThird HospitalBeijing 100191China Center for Reproductive Medicine Cheeloo College of MedicineShandong UniversityJinan 250012China Key Laboratory of Reproductive Endocrinology of Ministry of Education National Research Center for Assisted Reproductive Technology and Reproductive GeneticsShandong Key Laboratory of Reproductive MedicineShandong Provincial Clinical Research Center for Reproductive HealthJinan 250012China Key Laboratory of Organ Regeneration and Reconstruction Institute of ZoologyChinese Academy of SciencesBeijing 100101China Aging Biomarker Consortium Beijing 100101China

The ovary is indispensable for female reproduction,and its age-dependent functional decline is the primary cause of ***,the molecular basis of ovarian aging in higher vertebrates remains poorly ***,we apply spatiotemporal transcriptomics to benchmark architecture organization as well as cellular and molecular determinants in young primate ovaries and compare these to aged primate *** a global view,somatic cells within the non-follicle region undergo more pronounced transcriptional fluctuation relative to those in the follicle region,likely constituting a hostile microenvironment that facilitates ovarian ***,we uncovered that inflammation,the senescent-associated secretory phenotype,senescence,and fibrosis are the likely primary contributors to ovarian aging(PCOA).Of note,we identified spatial co-localization between a PCOA-featured spot and an unappreciated MT2(Metallothionein 2)highly expressing spot(MT2^(high))characterized by high levels of inflammation,potentially serving as an aging hotspot in the primate ***,with advanced age,a subpopulation of MT2^(high)accumulates,likely disseminating and amplifying the senescent signal *** study establishes the first primate spatiotemporal transcriptomic atlas,advancing our understanding of mechanistic determinants underpinning primate ovarian aging and unraveling potential biomarkers and therapeutic targets for aging and age-associated human ovarian disorders.

关键词： spatial transcriptome primate ovary aging senescence inflammation

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