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Nano Research 2025年第1期18卷 607-617页

作者： Ruo-Lin Jiang Hui-Na Liu Yu-Fan yang Zhi-Cheng Zhang Qi Dai xiao-yan Bao Lin-Jie Wu Ya-Xin Qin Xin Tan xiao-yan sun Xu-Fang Ying Zhi-Qing Ben Min Han Institute of Pharmaceutics College of Pharmaceutical SciencesZhejiang UniversityHangzhou 310058China Department of Radiation Oncology Key Laboratory of Cancer Prevention and InterventionThe Second Affiliated HospitalCollege of MedicineZhejiang UniversityHangzhou 310058China Hangzhou Institute of Innovative Medicine Zhejiang UniversityHangzhou 310058China Jinhua Institute of Zhejiang University Jinhua 321299China National Key Laboratory of Advanced Drug Delivery and Release Systems Zhejiang UniversityHangzhou 310058China Polytechnic institute Zhejiang UniversityHangzhou 310015China

Traditionally,hyaluronic acid has been widely used for drug delivery,but the current application bottleneck is that hyaluronic acid is hydrophilic and electronegative,which makes it difficult to carry hydrophobic drugs and small interfering RNA(siRNA)with the same *** on previous studies,we designed and synthesized hyaluronic acid nanocarriers HA-spermine/N,N,N-trimethylcystamine/DOX-TPP(HSTD)for loading siRNA to overcome the problem of siRNA release caused by strong electrostatic ***,N,N,Ntrimethylcystamine in the carrier can be degraded by intracellular glutathione to completely and rapidly release siRNA,thus promoting ***,when codelivered with the chemotherapy drug doxorubicin(DOX),this novel nanocarrier showed promising synergy in inhibiting tumor growth.

关键词： zwitterionic hyaluronic acid N N N-trimethylcystamine small interfering RNA(siRNA) doxorubicin

Role of periostin and its antagonist PNDA-3 in gastric cancer metastasis

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World Journal of Gastroenterology 2015年第9期21卷 2605-2613页

作者： Guo-xiao Liu Hong-Qing Xi xiao-yan sun Bo Wei Department of General Surgery Chinese People's Liberation Army General Hospital

The extracellular matrix component periostin is a secreted protein that functions as both a cell attachment protein and an autocrine or paracrine factor that signals through the cell adhesion molecule integrins αvβ3 and αvβ*** participates in normal physiological activities such as cardiac development,but is also involved in pathophysiological processes in vascular diseases,wound repair,bone formation,and tumor *** is of increasing interest in tumor biologybecause it is frequently overexpressed in a variety of epithelial carcinomas and is functionally involved in multiple steps of metastasis *** include the maintenance of stemness,niche formation,EMT,the survival of tumor cells,and angiogenesis,all of which are indispensable for gastric cancer *** has been reported to activate the PI-3K/AKT,Wnt,and FAK-mediated signaling pathways to promote ***,periostin represents a potentially promising candidate for the inhibition of *** this review article,we summarize recent advances in knowledge concerning periostin,its antagonist PNDA-3,and their influence on such key processes in cancer metastasis as maintenance of stemness,niche formation,epithelialto-mesenchymal transition,tumor cell survival,and *** particular,we focus our attention on the role of periostin in gastric cancer metastasis,speculate as to the usefulness of periostin as a therapeutic and diagnostic target for gastric cancer metastasis,and consider potential avenues for future research.

关键词： Periostin Cancer Tumorigenesis Extracellular matri

Transjugular intrahepatic portosystemic shunt is effective in patients with chronic portal vein thrombosis and variceal bleeding

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Hepatobiliary & Pancreatic Diseases International 2021年第2期20卷 128-136页

作者： xiao-yan sun Guang-Chuan Wang Jing Wang Guang-Jun Huang Chun-Qing Zhang Department of Gastroenterology Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityJinan 250021China Department of Infectious Diseases Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityJinan 250021China

Background:Studies about treatment of patients with chronic portal vein thrombosis(CPVT)are still limited,especially in different types of *** study aimed to evaluate the effect of transjugular intrahepatic portosystemic shunt(TIPS)in all types of CPVT with variceal ***:Patients with CPVT who received TIPS treatment between January 2011 and June 2019 were divided into four types according to the extent of *** patients had a history of variceal *** characteristics and clinical parameters were collected and *** on procedure success rate,variation in portal vein pressure,rebleeding,hepatic encephalopathy(HE),stent stenosis,and overall mortality were ***:A total of 189 patients were included in this study(39 in type 1,84 in type 2,48 in type 3,18 in type 4).The TIPS procedure success rate was 86.2%.The success rate was significantly different among the four types(89.7%vs.88.1%vs.83.3%vs.77.8%,P=0.001).In the TIPS success group,portal vein pressure was significantly reduced from 27.15±6.59 to 19.74±6.73 mm Hg after the procedure(P<0.001)and the rebleeding rate was significantly lower than that of the fail group(14.7%vs.30.8%,P=0.017).In addition,there were no significant differences in HE rate(30.7%vs.26.9%,P=0.912)or overall mortality(12.9%vs.19.2%,P=0.403)between the TIPS success group and the fail *** the TIPS success group,we found that the occurrence of HE was significantly different(P=0.020)among the four types,while there were no significant differences in rebleeding rate(P=0.669),stent stenosis rate(P=0.056),or overall mortality(P=0.690).Conclusions:TIPS was safe and effective in decreasing portal vein pressure and rebleeding rate in patients with CPVT.

关键词： Chronic portal vein thrombosis Transjugular intrahepatic portosystemic shunt Variceal bleeding Hepatic encephalopathy

Chemerin Exacerbates Psoriasis by Stimulating Keratinocyte Proliferation and Cytokine Production

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Current Medical Science 2023年第2期43卷 399-408页

作者： Shu-min KONG xiao-yan sun Wen-ya CUI Yu-chun CAO Department of Dermatology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China

Objective Psoriasis is often combined with metabolic abnormalities,such as obesity and *** upregulation of chemerin,which is an essential protein produced primarily from white fat,is strongly correlated to the development of ***,there is no clarification on its exact function and mechanism in disease *** present study aims to determine its function and mechanism in disease *** The present study used a psoriasislike inflammatory cell model and imiquimod(IMQ)-induced mouse model to confirm whether chemerin is upregulated in psoriasis *** Chemerin enhanced the keratinocyte proliferation,inflammatory cytokine secretion,and activation of the MAPK signaling ***,the intraperitoneal injection of neutralizing anti-chemerin antibody(ChAb)diminished the epidermal proliferation and inflammation in the IMQ-induced mouse *** The present results indicate that chemerin promotes keratinocyte proliferation,and enhances the production of inflammatory cytokines,thereby aggravating the ***,chemerin can be a prospective target for the treatment of psoriasis.

关键词： psoriasis keratinocyte chemerin proliferation inflammation

Effect of radio frequency bias on plasma characteristics of inductively coupled argon discharge based on fluid simulations

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Chinese Physics B 2020年第9期29卷 369-376页

作者： xiao-yan sun Yu-Ru Zhang Sen Chai You-Nian Wang yan-yan Chu Jian-Xin He Textile and Garment Industry of Research Institute Zhongyuan University of TechnologyZhengzhou 450007China Key Laboratory of Materials Modification by Laser Ionand Electron Beams(Ministry of Education)School of PhysicsDalian University of TechnologyDalian 116024China China Special Equipment Inspection and Research Institute Beijing 100029China

t A fluid model is employed to investigate the effect of radio frequency bias on the behavior of an argon inductively coupled plasma(ICP).In particular,the effects of ICP source power,single-frequency bias power,and dual-frequency bias power on the characteristics of ICP are simulated at a fixed pressure of 30 mTorr(1 Torr=1.33322×102 Pa).When the bias frequency is fixed at 27.12 MHz,the two-dimensional(2D)plasma density profile is significantly affected by the bias power at low ICP source power(e.g.,50 W),whereas it is weakly affected by the bias power at higher ICP source power(e.g.,100 W).When dual-frequency(27.12 MHz/2.26 MHz)bias is applied and the sum of bias powers is fixed at 500 W,a pronounced increase in the maximum argon ion density is observed with the increase of the bias power ratio in the absence of ICP source *** the ratio of 27.12-MHz/2.26-MHz bias power decreases from 500 W/0 W to 0 W/500 W with the ICP source power fixed at 50 W,the plasma density profiles smoothly shifts from edge-high to center-high,and the effect of bias power on the plasma distribution becomes weaker with the bias power ratio ***,the axial ion flux at the substrate surface is characterized by a maximum at the edge of the *** the ICP source power is higher,the 2D plasma density profiles,as well as the spatiotemporal and radial distributions of ion flux at the substrate surface are characterized by a peak in the reactor center,and the distributions of plasma parameters are negligibly affected by the dual-frequency bias power ratio.

关键词： fluid simulation single-and dual-frequency bias power plasma distribution

Small molecules facilitate single factor-mediated sweat gland cell reprogramming

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Military Medical Research 2022年第6期9卷 655-667页

作者： Shuai-Fei Ji Lai-Xian Zhou Zhi-Feng sun Jiang-Bing Xiang Shao-Yuan Cui yan Li Hua-Ting Chen Yi-Qiong Liu Huan-Huan Gao xiao-Bing Fu xiao-yan sun Research Center for Tissue Repair and Regeneration Affiliated To Medical Innovation Research Department and 4th Medical Center PLA General Hospital and PLA Medical CollegePLAKey Laboratory of Tissue Repairand Regenerative Medicine and BeijingKey Research Laboratoryof Skin InjuryRepair and Regeneration28 FuXing RoadBeijing 100853China Research Unit of Trauma Care Tissue Repair and RegenerationChinese Academy of Medical Sciences2019RU051Beijing 100048China Departmentof Respiratory the Second Medical CenterChinese PLA General HospitalBeijing 100036China Bioengineering College of Chongqing University Chongqing 400044China Department of Nephrology the First Medical CenterChinese PLA General HospitalState Key Laboratory of Kidney DiseasesBeijing 100048China

Background: Large skin defects severely disrupt the overall skin structure and can irreversibly damage sweat glands(SGs), thus impairing the skin’s physiological function. This study aims to develop a stepwise reprogramming strategy to convert fibroblasts into SG lineages, which may provide a promising method to obtain desirable cell types for the functional repair and regeneration of damaged ***: The expression of the SG markers cytokeratin 5(CK5), cytokeratin 10(CK10), cytokeratin 18(CK18), carcinoembryonic antigen(CEA), aquaporin 5(AQP5) and α-smooth muscle actin(α-SMA) was assessed with quantitative PCR(qPCR), immunofluorescence and flow cytometry. Calcium activity analysis was conducted to test the function of induced SG-like cells(iSGCs). Mouse xenograft models were also used to evaluate the in vivo regeneration of ***/c nude mice were randomly divided into normal group, SGM treatment group and iSGC transplantation *** analyses and starch-iodine sweat tests were used to confirm the in vivo regeneration of ***: Ectodermal dysplasia antigen(EDA) overexpression drove human dermal fibroblast(HDF) conversion into i SGCs in SG culture medium(SGM). qPCR indicated significantly increased mRNA levels of the SG markers CK5, CK18and CEA in iSGCs, and flow cytometry data demonstrated(4.18±0.04)% of iSGCs were CK5 positive and(4.36±0.25)%of iSGCs were CK18 positive. The addition of chemical cocktails greatly accelerated the SG fate program. qPCR results revealed significantly increased mRNA expression of CK5, CK18 and CEA in iSGCs, as well as activation of the duct marker CK10 and luminal functional marker AQP5. Flow cytometry indicated, after the treatment of chemical cocktails,(23.05±2.49)% of iSGCs expressed CK5^(+) and(55.79±3.18)% of iSGCs expressed CK18^(+), respectively. Calcium activity analysis indicated that the reactivity of iSGCs to acetylcholine was close to that of primary SG cells [(60.79±7.71)% vs.(70.59±0.34)%

关键词： Direct reprogramming Human dermal fibroblasts Sweat gland Regeneration

CCPG1 involved in corneal Aspergillus fumigatus infection

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International Journal of Ophthalmology(English edition) 2022年第4期15卷 541-546页

作者： Li-Mei Wang xiao-Meng Chen Hai-Jing yan Shu yan xiao-yan sun Da-Wei Zhang Hua yang Dan-Li Lu Cheng-Ye Che Department of Ophthalmology the Affiliated Hospital of Qingdao UniversityQingdao 266000Shandong ProvinceChina Department of Otorhinolaryngology-Head and Neck Surgery the Affiliated Hospital of Qingdao UniversityQingdao 266000Shandong ProvinceChina Department of Emergency the Affiliated Hospital of Qingdao UniversityQingdao 266000Shandong ProvinceChina

AIM: To investigate whether non-canonical autophagy transport receptor cell cycle progression 1(CCPG1) is involved in the corneal antifungal immune ***: Human corneal epithelial cells(HCECs) and human myeloid leukemia mononuclear cells(THP-1) macrophages stimulated by Aspergil us fumigatus(A. fumigatus) were used as cell models. The expression of CCPG1 m RNA was detected by q RT-PCR. Western blot was used to determine the protein expression of CCPG1 and interleukin-1β(IL-1β). The dectin-1 neutralizing antibody was used to detect the association between dectin-1 and CCPG1. Immunofluorescence was used to observe the colocalization of CCPG1 and C-type lectin-like receptor-1(CLEC-1) in THP-1 ***: The expression of CCPG1 started to increase at 4 h after infection and increased in a time-dependent manner in HCECs and THP-1 macrophages. With dectin-1 neutralizing antibody pretreatment, the expression of IL-1β was down-regulated. CCPG1 up-regulation in response to A. fumigatus infection was independent of dectin-1. Immunofluorescence showed the colocalization of CCPG1 and CLEC-1 in THP-1 ***: As a specific autophagy protein of noncanonical autophagy pathway, CCPG1 is involved in corneal infection with A. fumigatus.

关键词： cell cycle progression 1 fungal keratitis Aspergillus fumigatus C-type lectin-like receptor-1

Idiopathic neonatal pneumoperitoneum with favorable outcome: A case report and review

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World Journal of Gastroenterology 2015年第20期21卷 6417-6421页

作者： Tao-Zhen He Chang Xu Yi Ji xiao-yan sun Min Liu Department of Pediatric Surgery West China Hospital of Sichuan University Health Management Centre West China Hospital of Sichuan University

Neonatal pneumoperitoneum is a surgical emergency indicative of gastrointestinal perforation that requires immediate treatment to prevent death. There have been non-surgical conditions secondary to neonatal pneumoperitoneum(e.g., mechanical ventilation, pulmonary diseases and pneumatosis cystoides intestinalis) that neonates were able to overcome without the need for abdominal exploration. Idiopathic pneumoperitoneum, although similar to perforation of the alimentary tract and the previously mentioned non-surgical conditions, is a more rare and benign condition that does not yet have a definite cause. Hence, inexperienced surgeons may have a difficult time providing the right treatment for idiopathic pneumoperitoneum. We report a case of a neonate with a massive pneumoperitoneum who obtained a favorable outcome without surgical intervention. Nonetheless, the cause of pneumoperitoneum remains unclear. We hypothesize that the right sized perforation(range: 2 mm to 4 mm in diameter) at the anterior wall of the stomach is needed for pneumoperitoneum to occur. As the baby cries(aerophagia), the air in the stomach accumulates until it can enter the intraperitoneal cavity through the leak compressed by gastric peristalsis, hence forming a large pneumoperitoneum. Small amounts of gastric juice are able to penetrate the gastric wall; therefore, no signs or symptoms of peritonitis occur. The gastric leak self-seals, preventing further passage of the air, allowing the intraperitoneal free gas to dissipate gradually. This case demonstrated that laparotomy can be avoided in neonates with idiopathic pneumoperitoneum if a timely diagnosis is established.

关键词： Intestinal perforation Pneumoperitoneum Newborn Therapeutics

Fluid simulation of the pulsed bias effect on inductively coupled nitrogen discharges for low-voltage plasma immersion ion implantation

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Chinese Physics B 2017年第1期26卷 290-297页

作者： xiao-yan sun Yu-Ru Zhang Xue-Chun Li You-Nian Wang Key Laboratory of Materials Modification by Laser Ion and Electron Beams （Ministry of Education） School of Physics and Optoelectronic Technology Dalian University of Technology Dalian 116024 China

Planar radio frequency inductively coupled plasmas（ICP） are employed for low-voltage ion implantation processes,with capacitive pulse biasing of the substrate for modulation of the ion energy. In this work, a two-dimensional（2D） selfconsistent fluid model has been employed to investigate the influence of the pulsed bias power on the nitrogen plasmas for various bias voltages and pulse frequencies. The results indicate that the plasma density as well as the inductive power density increase significantly when the bias voltage varies from 0 V to-4000 V, due to the heating of the capacitive field caused by the bias power. The N＋fraction increases rapidly to a maximum at the beginning of the power-on time, and then it decreases and reaches the steady state at the end of the glow period. Moreover, it increases with the bias voltage during the power-on time, whereas the N2-＋ fraction exhibits a reverse behavior. When the pulse frequency increases to 25 kHz and40 kHz, the plasma steady state cannot be obtained, and a rapid decrease of the ion density at the substrate surface at the beginning of the glow period is observed.

关键词： fluid simulation low-voltage plasma immersion ion implantation N2 inductive discharge

Cloning and Overexpression of CYP6F1,a Cytochrome P450 Gene,from Deltamethrin-resistant Culex pipiens pallens

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Acta Biochimica et Biophysica Sinica 2005年第5期37卷 317-326页

作者： Mao-Qing GONG yan GU xiao-Bang HU yan sun Lei MA Xiu-Lan LI Li-Xin sun Jing sun Jin QIAN Chang-Liang ZHU Department of Pathogenic Biology, Nanjing Medical University: Key Laboratory of Modern Pathogenic Biology of Jiangsu Province, Nanjing 210029, China Department of Pathogenic Biology Nanjing Medical University Key Laboratory of Modern Pathogenic Biology of Jiangsu ProvinceNanjing 210029 China

CYP6F1(GenBank/EMBL accession ***662654),a novel gene with a complete encodingsequence in the cytochrome P450 family 6,was cloned and sequenced from deltamethrin-resistant 4thinstar larvae of Culex pipiens *** cDNA sequence of CYP6F1 has an open reading frame of 1527bp,which encodes a putative protein of 508 amino acid *** deduced amino acid sequence ofCYP6F1 indicated that the encoded P450 has conserved domains of a putative membrane-anchoring signal,putative reductase-binding sites,a typical heme-binding site,an ETLR motif and substrate recognition ***-quantitative RT-PCR analysis indicated that the CYP6F1 gene was expressed to a greater extent in thedeltamethrin-resistant strain than in the susceptible strain of *** *** expression levels ofthe CYP6F1 gene in the deltamethrin-resistant 1 st,2nd,3rd,4th instar larvae and adult female mosquitoesdiffered,with highest expression levels in the 4th instar *** addition,the CYP6F1 gene was stablyexpressed in mosquito C6/36 cells,and the expected 61.2 kDa band was identified by Western *** transfected with CYP6F1 had an increased resistance to deltamethrin as compared with control *** results indicate that CYP6F1 is expressed at higher levels in the deltamethrin-resistant strain,and mayconfer some insecticide resistance in *** pallens.

关键词： Culex pipiens pallens insecticide resistance cytochrome P450 RT-PCR