检索结果-南通市图书馆

Physcion increases the sensitivity of hepatocellular carcinoma to sorafenib through miRNA-370/PIM1 axis-regulated glycolysis

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastrointestinal Oncology 2023年第8期15卷 1400-1411页

作者： xiao-ping pan Bu-Ren Jiya Feng Wang Zhu Lan Department of Interventional Radiology Inner Mongolia International Mongolian HospitalHohhot 016000Inner Mongolia Autonomous RegionChina Graduate School Inner Mongolia Medical UniversityHohhot 016000Inner Mongolia Autonomous RegionChina

BACKGROUND Resistance to sorafenib has become a challenge in clinical treatment of hepatocellular carcinoma(HCC).Physcion is a common bioactive anthraquinone that has potential as an anticancer agent.AIM To study the effect of physcion on sensitizing HCC cells to sorafenib.METHODS Sorafenib-resistant HCC cells were established and treated with sorafenib and/or physcion.The cell viability,proliferation and apoptosis were measured by cell counting kit-8,colony formation,flow cytometry,and in vivo xenograft model.Glucose uptake,lactate acid production,extracellular acidification rate(ECAR),and oxygen consumption rate(OCR)were measured to analyze glycolysis.Expression of glycolysis-related regulators was assessed by western blotting.RESULTS The addition of physcion significantly enhanced the antitumor effects of sorafenib on sorafenib-resistant HCC cells,manifested by enhanced apoptosis and suppressed cell growth.The glucose uptake,lactate acid production,and ECAR were elevated,and OCR was suppressed by physcion treatment.The level of PIM1 was elevated and miR-370 was suppressed in sorafenib-resistant HCC cells compared with the parental cells,which was suppressed by physcion treatment.Inhibition of miR-370 notably reversed the effects of physcion on sorafenib-resistant HCC cells.CONCLUSION Our data indicated that physcion enhanced the sensitivity of HCC cells to sorafenib by enhancing miR-370 to suppress PIM1-promoted glycolysis.

关键词： Hepatocellular carcinoma Sorafenib resistance Physcion Glycolysis PIM1

Estrogen receptor a gene PvulI polymorphism and coronary artery disease： a meta-analysis of 21 studies

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2014年第3期15卷 243-255页

作者： Jie DING Hui XU Xiang YIN Fu-rong ZHANG xiao-ping pan Yi-an GU Jun-zhu CHEN xiao-gang GUO Department of Cardiology the First Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310003 China Xiuzhou District Gaozhao Street Community Health Service Center Jiaxing 314031 China State Key Laboratory for Diagnosis and Treatment of Infectious Diseases the First Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310003 China Columbia University Medical Center New York NY l0032 USA

The association between the estrogen receptor α gene (ESR1) PvuII polymorphism (c.454-397T>C) and coronary artery disease (CAD) is controversial. Thus, we conducted a meta-analysis to evaluate the relationship. Data were collected from 21 studies encompassing 9926 CAD patients and 16710 controls. Odds ratios (ORs) with 95% confidence intervals (CIs) were used to assess the relationship between PvuII polymorphism and CAD. The polymorphism in control populations in all studies followed Hardy-Weinberg equilibrium. We found a significant association between ESR1 PvuII polymorphism and CAD risk in all subjects. When the data were stratified by region, a significant association between ESR1 PvuII polymorphism and CAD risk was observed in Asian populations but not in Western populations. The current study suggests that ESR1 PvuII polymorphism has an important role in CAD susceptibility.

关键词： Estrogen receptor α gene Polymorphism Meta-analysis Coronary artery disease (CAD) R541.4

Levels of vaccination coverage among HIV-exposed children in China: a retrospective study

在线全文

学校读者我要写书评

暂无评论

Infectious Diseases of Poverty 2021年第2期10卷 98-99页

作者： Rui Shen Ai-Ling Wang xiao-ping pan Ya-ping Qiao Qian Wang xiao-Yan Wang Shui-Ling Qu Tong Zhang National Center for Women and Children's Health Chinese Center for Disease Control and PreventionNo.12 Dahuisi RoadHaidian DistrictBeijing 100081China 不详 Capital Institute of Pediatrics No.2 Yabao RoadChaoyang DistrictBeijing 100020China

Background:Vaccination is crucial for human immunodeficiency virus(HlV)-exposed children because of their increased risk of morbidity and mortality from various vaccine-preventable diseases.However,studies have shown that they are at high risk of incomplete vaccination.Although China has developed prevention of mother-to-child transmission(PMTCT)of HIV programs substantially over the past decades,few studies have investigated the immunization levels of Chinese HIV-exposed children.Therefore,we aimed to evaluate vaccination coverage and its associated factors among HIV-exposed children in China during 2016-2018.

关键词： HIV Children Coverage Vaccination China

Regulation of Light Energy Utilization and Distribution of Photosynthesis in Five Subtropical Woody Plants

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2007年第3期49卷 351-361页

作者： Nan Liu Chang-Lian Peng Zhi-Fang Lin Gui-Zhu Lin xiao-ping pan South China Botanical Garden the Chinese Academy of Sciences Guangdong Key Laboratory of Digital Botanical Garden Guangzhou 510650 China College of Life Sciences South China Normal University Guangzhou 510631 China Graduate University of the Chinese Academy of Sciences Beijing 100049 China

The adaptations and responses of photosynthesis to long- and short-term growth light gradient treatments were investigated in five subtropical forest plants, namely Pinus massoniana Lamb., Schima superba Gardn. et Champ., Castanopsis flssa （Champ. ex Benth.） Rehd. et Wils., Acmena acuminatissima （BI.） Merr et Perry, and Cryptocarya concinna Hance. With diurnal changes in sunlight and air temperature, the de-epoxidation state and lutein content in the five woody plants under three light Intensities first increased and then decreased during the day. However, maximal photochemical efficiency （FvFm; where Fm is the maximum fluorescence yield and Fv Is variable fluorescence） and the photochemical quantum yields of photosystem （PS）Ⅱ （φPSⅡ） of the species examined changed in the opposite manner, with those in plants grown under 100% natural light changing the most. After long-term treatment （21 months）, anti-oxidant capacity （1,1-diphenyl-2-picrylhydrazyl radical （DPPH.）-scavenging capacity） and utilization of excitation energy showed differences in modulation by different light intensities. It was shown that A. acuminatissima and C. concinna, as dominant species in the late succession stage of a subtropical forest in Dinghu mountain, South China, were better able to adapt to different light environments. However, P. massonlana, the pioneer species of this forest, exhibited less adaptation to low light intensity and was definitely eliminated by the forest succession process.

关键词： adaptation chlorophyll fluorescence community succession light intensity subtropical forest

Cost-effectiveness analysis of the prevention of mother-to-child transmission of HIV

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Infectious Diseases of Poverty 2022年第3期11卷 97-97页

作者： Shui-ling Qu Ai-ling Wang Hong-mei Yin Jin-qi Deng xiao-yan Wang Ye-huan Yang xiao-ping pan Tong Zhang 不详 National Center for Women and Children’s Health Chinese Center for Disease Control and PreventionNo.12 Dahuisi RoadHaidian DistrictBeijing100081China Capital Institute of Pediatrics No.2 Yabao RoadChaoyang DistrictBeijing100020China

Background:The number of HIV-positive pregnant women accounted for about 10% of China’s total over the past few years in Liangshan Prefecture, Sichuan province in China. Although cost-effectiveness of the PMTCT of HIV have been evaluated in other previous studies, no specific study has been conducted in Liangshan prefecture, nor has the expenses paid individually by HIV-positive pregnant women been included. The purpose of this study was to evaluate both the short-term and long-term cost-effectiveness of PMTCT of HIV in Liangshan Prefecture from the social perspective.Methods:From December 2018 to January 2019, individual expenses and the other costs were collected: individual expenses of 133 recruited HIV-positive pregnant women registered in the National Information System of Prevention of Mother-to-Child Transmission of HIV, Syphilis, and HBV, and the other costs from local maternal and child healthcare hospitals, Centers for Disease Control and Prevention, and general hospitals. The costs, the number of pediatric infections averted from being HIV infected were analyzed. And, Life years gained by pediatric infections averted were calculated by using a life table. Besides, Direct benefit was calculated through a Markov mode. Furthermore, One-way sensitivity analysis was conducted for key variables affecting the benefit–cost ratio.Results:The estimated number of pediatric infections averted was 164.The total cost was USD 114.1 million, including direct medical costs, direct non-medical costs, and indirect costs, which were USD 54.2 million, USD 53.4 million, and USD 6.5 million, respectively. 630.6 person-years discounted to 2017 were gained at a 3% annual rate, and cost per life year gained was USD 1809.50. Direct benefits were USD 198.4 million, indirect benefits USD 82.5 million, and the benefit–cost ratio was 1.5. The sensitivity analysis showed that if PMTCT costs hypothetically ranged from USD 85.6 million to USD 142.6 million, benefit–cost ratio would vary from 1.0 to 2.3.Conclusions:PMTCT of HIV in Liang

关键词： Cost-effectiveness PMTCT HIV Benefit-cost ratio Individual cost

Changes in Photosystem Ⅱ Activity and Leaf Reflectance Features of Several Subtropical Woody Plants Under Simulated SO2 Treatment

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2006年第11期48卷 1274-1286页

作者： Nan Liu Chang-Lian Peng Zhi-Fang Lin Gui-Zhu Lin Ling-Ling Zhang xiao-ping pan South China Botanical Garden the Chinese Academy of Sciences Guangdong Key Laboratory of Digital Botanical Garden Guangzhou 510650 China College of Life Sciences South China Normal University Guangzhou 510631 China Graduate University of the Chinese Academy of ScienceS Beijing 100049 China）

The effects of simulated SO2 treatment on the photosynthetic apparatus were investigated in five subtropical forest plants, namely Plnus massonlana Lamb., Schlma superba Gardn. et Champ., Castanopsls flssa （Champ. ex Benth.） Rehd. et Wils., Acmena acuminatissima （BI.） Merr et Perry, and Cryptocarya concinna Hance. After leaf sections had been immersed in 0, 20, 50, and 100 mmol/L NaHSO3 for 20 h, total chlorophyll （Chl） content, Chl a/b, maximal photochemical efficiency, and the photochemical quantum yields of photosystem Ⅱ of all five woody plants were reduced to different degrees, whereas lutein content （Chl base） was increased. Two protective mechanisms, namely the xanthophyll cycle （de-epoxidation） and an anti-oxidant system （1,1-diphenyl-2-picrylhydrazyl radical-scavenging capacity）, showed differences in the degree of modulation under simulated SO2 treatment. Compared with control （distilled water treatment）, the revised normalized difference vegetation index, a leaf reflectance index, was lowered with Increasing concentrations of NaHSO3. Cryptocarya conclnna, a dominant species in the late succession stage of subtropical forests in South China, exhibited less sensitivity to NaHSO3. Conversely, Pinus massonlana, the pioneer hellophyte species, was most susceptible to NaHSO3 treatment. It Is suggested that SO2 pollution may accelerate the succession of subtropical forest.

关键词： community succession light intensity NariSO3 subtropical forest,

ERCP术后胆道感染危险因素及其预测模型构建

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中华医院感染学杂志 2024年第1期34卷 63-66页

作者：肖盼平王海英张堃史奕杨琳空军军医大学第一附属医院消化六科陕西西安710038

目的探讨内镜逆行胰胆管造影术(ERCP)术后并发胆道感染病原菌分布、危险因素,并构建预测模型。方法选取2020年10月-2022年10月于空军军医大学第一附属医院行ERCP术患者1040例为研究对象,根据患者术后是否并发胆道感染分为并发胆道感染... 详细信息

目的探讨内镜逆行胰胆管造影术(ERCP)术后并发胆道感染病原菌分布、危险因素,并构建预测模型。方法选取2020年10月-2022年10月于空军军医大学第一附属医院行ERCP术患者1040例为研究对象,根据患者术后是否并发胆道感染分为并发胆道感染组30例和未并发胆道感染组1010例,收集患者临床资料,分析胆道感染病原菌分布及其危险因素,并构建预测模型,绘制受试者工作特征(ROC)曲线分析模型的预测价值。结果1040例ERCP术后患者并发胆道感染30例,发生率为2.88%,共培养病原菌42株,以大肠埃希菌为主,其次为粪肠球菌;胆道梗阻位置、ERCP术后未预防性使用抗菌药物、合并糖尿病是ERCP术后并发胆道感染的独立危险因素(P11时,AUC值为0.843,敏感度为86.67%,特异度为67.43%。结论ERCP术后并发胆道感染病原菌以革兰阴性菌为主,危险因素包括胆道梗阻位置高位、ERCP术后未预防性使用抗菌药物、合并糖尿病,据此构建预测模型的预测价值较高。

关键词：内镜逆行胰胆管造影胆道感染病原菌危险因素预测模型

Efficient generation of functional hepatocytelike cells from mouse liver progenitor cells via indirect co-culture with immortalized human hepatic stellate cells

在线全文

学校读者我要写书评

暂无评论

Hepatobiliary & pancreatic Diseases International 2016年第2期15卷 173-179页

作者： xiao-ping pan Yi-Ni Wang xiao-Peng Yu Chun-Xia Zhu Jian-Zhou Li Wei-Bo Du Yi-Min Zhang Hong-Cui Cao Yan-Hong Zhang Dan-Hua Zhu George C Yeoh Lan-Juan Li Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases State Key Laboratory for Diagnosis and Treatment of Infectious Diseases First Affiliated Hospital Zhejiang University School of Medicine College of Basic Medical Sciences Zhejiang Chinese Medical University Biochemistry & Molecular Biology Crawley University of Western Australia

BACKGROUND： Differentiation of liver progenitor cells（LPCs） to functional hepatocytes holds great potential to develop new strategies for hepatocyte transplantation and the screening of drug-induced cytotoxicity. However, reports on the efficient and convenient hepatic differentiation of LPCs to hepatocytes are few. The present study aims to investigate the possibility of generating functional hepatocytes from LPCs in an indirect co-culture system.METHODS： Mouse LPCs were co-cultured in Transwell plates with an immortalized human hepatic stellate cell line（HSCLi） we previously established. The morphology, expression of hepatic markers, and functions of mouse LPC-derived cells were monitored and compared with those of conventionally cultured LPCs. RESULTS： Co-culturing with HSC-Li cells induced differentiation of mouse LPCs into functional hepatocyte-like cells. The differentiated cells were morphologically transformed into hepatocyte-like cells 3 days after co-culture initiation. In addition, the differentiated cells expressed liver-specific genes and possessed hepatic functions, including glycogen storage, lowdensity lipoprotein uptake, albumin secretion, urea synthesis, and cytochrome P450 1A2 enzymatic activity.CONCLUSIONS： Our method, which employs indirect co-culture with HSC-Li cells, can efficiently induce the differentiation of LPCs into functional hepatocytes. This finding suggests that this co-culture system can be a useful method for the efficient generation of functional hepatocytes from LPCs.

关键词： liver progenitor cells indirect co-culture cell differentiation hepatocytes immortalized human hepatic stellate cells

Evaluation of a novel choanoid fluidized bed bioreactor for future bioartificial livers

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2014年第22期20卷 6869-6877页

作者： Cheng-Bo Yu xiao-ping pan Liang Yu xiao-Peng Yu Wei-Bo Du Hong-Cui Cao Jun Li ping Chen Lan-Juan Li States Key Laboratory for Diagnosis and Treatment of Infectious Diseases First Affiliated HospitalSchool of MedicineZhejiang University Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases

AIM:To construct and evaluate the functionality of a choanoid-fluidized bed bioreactor(CFBB)based on microencapsulated immortalized human hepatocytes.METHODS:Encapsulated hepatocytes were placed in the constructed CFBB and circulated through Dulbecco’s Modified Eagle’s Medium(DMEM)for 12 h,and then through exchanged plasma for 6 h,and compared with encapsulated cells cultivated under static conditions in a spinner flask.Levels of alanine aminotransferase(ALT)and albumin were used to evaluate the CFBB during media circulation,whereas levels of ALT,total bilirubin(TBil),and albumin were used to evaluate it during plasma circulation.Mass transfer and hepatocyte injury were evaluated by comparing the results from the two experimental conditions.In addition,the viability and microstructure of encapsulated cells were observed in the different environments.RESULTS:The bioartificial liver model based on a CFBB was verified by in vitro experiments.The viability of encapsulated cells accounting for 84.6%±3.7%in CFBB plasma perfusion was higher than the 74.8%±3.1%in the static culture group(P0.05)at 6 h in the CFBB plasma perfusion and static plasma culture groups,respectively.There was no significant difference in albumin concentration between the two experimental plasma groups at any time point.The microstructure of the encapsulated hepatocytes remained healthier in the CFBB group compared with the static culture group after 6 h of plasma perfusion.CONCLUSION:The CFBB can function as a bioartificial liver based on a bioreactor.

关键词： Choanoid Fluidized bed Bioreactor Im-mortalized hu