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Chinese Journal of Cancer Research 2016年第1期28卷 29-49页

作者： Qiu Rao Qiu-Yuan Xia Liang Cheng xiao-jun zhou Department of Pathology Jinling Hospital Nanjing University School of Medicine Nanjing China Department of Pathology and Laboratory Indiana University School of Medicine Indianapolis IN USA

Renal cell carcinoma （RCC） compromises multiple types and has been emerging dramatically over the recent several decades. Advances and consensus have been achieved targeting common RCCs, such as clear cell carcinoma, papillary RCC and chromophobe RCC. Nevertheless, little is known on the characteristics of several newly-identified RCCs, including clear cell （tubulo） papillary RCC, Xpl 1 translocation RCC, t（6;11） RCC, succinate dehydrogenase （SDH）-deficient RCC, acquired cystic disease- associated RCC, hereditary leiomyomatosis RCC syndrome-associated RCC, ALK translocation RCC, thyroid-like follicular RCC, tubulocystic RCC and hybrid oncocytic/chromophobe tumors （HOCT）. In current review, we will collect available literature of these newly-described RCCs, analyze their clinical pathologic characteristics, discuss their morphologic and immunohistologic features, and finally summarize their molecular and genetic evidences. We expect this review would be beneficial for the understanding of RCCs, and eventually promote clinical management strategies.

关键词： Renal cell carcinoma (RCC) renal tumor immunohistochemistry molecular genetics

The whole-genome survey of Acer griseum,its polymorphic simple sequence repeats development and application

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BIOCELL 2023年第8期47卷 1907-1913页

作者： xiao-jun zhou YU-WEI TIAN RUI-HAN LI Life Science College Luoyang Normal UniversityLuoyang471934China The Second Institute of Resources and Environment Investigation of Henan Province Luoyang471000China

Background:Acer griseum Pax is an endangered species endemic to China with both ornamental and economic ***,the lack of information on its genome size and characteristics hinders further work at the genome ***:This paper applied bioinformatics methods to predict the characteristics and patterns of the *** genome,providing an important basis for formulating its whole-genome sequencing *** study also characterized the simple sequence repeats(SSRs)of ***,laying the foundation for the development and application of genome-wide SSR *** this study,PE150 sequencing was performed by the BGI MGISEQ platform,and the sequence files were analyzed by the K-mer method to estimate the characteristic information by GCE ***:The genome size was finally determined to be 739.63 Mb,its heterozygosity ratio was 1.33%,and the repetition ratio was 65.68%.A total of 825,960 SSR loci were identified in the assembled genome sequence,and primers were successfully designed for 526,020 *** verify the effectiveness of these primers,100 pairs of primers were randomly selected and synthesized,and 81 pairs successfully amplified the target *** pairs of primers with good polymorphism were selected for principal component analysis of 31 *** individuals from two populations,showing favorable heterozygosity and PIC *** to the findings,these SSRs might identify genetic variations based on geographic ***:It is suggested that Illumina+PacBio assembly strategy should be used for whole-genome sequencing due to the high heterozygosity rate and high repetition rate of the *** addition,the SSR primers designed in batches in this study laid a foundation for the in-depth study of population structure and population maintenance mechanism of ***,which is helpful for the effective conservation and sustainable utilization of this germplasm resource.

关键词： Endangered plant Paperbark maple Genome Genetic conservation

Nonlinearity-Compensation-Free Optical Frequency Domain Reflectometry Based on Electrically-Controlled Optical Frequency Sweep

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Journal of Electronic Science and Technology 2021年第1期19卷 89-96页

作者： Fan Yang Ling-Jie Zhang Zhi-Yao Zhang xiao-jun zhou Yong Liu the School of Optoelectronic Science and Engineering University of Electronic Science and Technology of ChinaChengdu 610054

A nonlinearity-compensation-free optical frequency domain reflectometry(OFDR)scheme is proposed and experimentally demonstrated based on the electrically-controlled optical frequency *** the proposed scheme,the linear frequency sweep light is generated by propagating an ultra-narrow-linewidth continuous-wave(CW)light through an electro-optic frequency shifter which consists of a dual-parallel Mach-Zehnder modulator(DPMZM)and an electronic 90°hybrid,where the electro-optic frequency shifter is driven by a linear frequency modulated signal generated by a direct digital synthesizer(DDS).Experimental results show that the spatial resolution and signal-to-noise ratio(SNR)of the proposed OFDR scheme without the nonlinear phase compensation are comparable to those of OFDR employing a commercial tunable laser source(TLS),an auxiliary interferometer,and a software-based nonlinear phase compensation *** proposed OFDR scheme is helpful to reduce the complexity of the optical structure and eliminate the difficulty of developing the nonlinear phase compensation algorithm.

关键词： Dual-parallel Mach-Zehnder modulator(DPMZM) electro-optic frequency shifter optical fiber sensor optical frequency domain reflectometry(OFDR)

Characterizing the composition of intestinal microflora by 16S rRNA gene sequencing

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World Journal of Gastroenterology 2020年第6期26卷 614-626页

作者： Wen-Jia Wang You-Lian zhou Jie He Zhi-Qiang Feng Long Zhang xiao-Bo Lai jun-xiao zhou Hong Wang Department of Gastroenterology Guangzhou Digestive Disease CenterGuangzhou First People’s Hospital South China University of Technology

BACKGROUND This study determined the composition and diversity of intestinal microflora in patients with colorectal adenoma(CRA),which may provide precedence for investigating the role of intestinal microflora in the pathogenesis of colorectal tumors,the composition of intestinal microflora closely related to CRA,and further validating the possibility of intestinal flora as a biomarker of *** To study the relationship between intestinal microflora and *** This is a prospective control case study from October 2014 to june 2015 involving healthy volunteers and patients with advanced ***-throughput sequencing and bioinformatics analysis were used to investigate the composition and diversity of intestinal microflora in 36 healthy subjects and 49 patients with advanced *** measured were operational taxonomic units of intestinal flora,as well as their abundance and diversity(αandβtypes).RESULTS In this study,the age,gender,body mass index,as well as location between controls and patients had no significant *** mucosa-associated gut microbiota diversity and bacterial distribution in healthy controls and colorectal adenomas were *** operational taxonomic unit,abundance,andαandβdiversity were all reduced in patients with CRA compared to *** the phylum level,the composition of intestinal microflora was comparable between patients and controls,but the abundance of Proteobacteria was increased,and Firmicutes and Bacteroides were significantly decreased(P<0.05).The increase in Halomonadaceae and Shewanella algae,and reduction in Coprococcus and Bacteroides ovatus,could serve as biomarkers of ***-throughput sequencing confirms the special characteristics and diversity of intestinal microflora in healthy controls and patients with *** The diversity of intestinal microflora was decreased in patients with *** increase in Halomonadaceae and Shewanella algae are markers of CRA.

关键词： 16S rDNA Intestinal microflora Advanced colorectal adenoma Colorectal cancer Biomarkers

Over-expression of uPA increases risk of liver injury in pAAV-HBV transfected mice

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World Journal of Gastroenterology 2012年第16期18卷 1892-1902页

作者： xiao-jun zhou Shi-Hui Sun Peng Wang Hong Yu Jing-Ya Hu Shi-Cheng Shang Yu-Sen zhou Beijing Institute of Microbiology and Epidemiology Beijing 100071 China Beijing Experimental Animal Center Beijing 100071 China

AIM:To investigate the relationship between overexpression of urokinase plasminogen activator(uPA) and hepatitis B virus(HBV) related liver diseases in a transgenic mouse ***:Albumin-tetracycline reverse transcriptional activator and tetO-uPA transgenic mice were generated respectively through pronuclear injection and crossed to produce the double transgenic in-alb-uPA mice,for which doxycycline(Dox)-inducible and liver-specific over-expression of uPA can be *** transfection of plasmid adeno-associated virus(AAV)1.3HBV was performed through the tail veins of the Dox-induced in-alb-uPA *** of uPA and HBV antigens were analyzed through double-staining immunohistochemical *** production was detected by enzyme linked immunosorbent assay and α-fetoprotein(AFP) mRNA level was evaluated through real-time quantitative polymerase chain ***:Plasmid AAV-1.3HBV hydrodynamic transfection in Dox-induced transgenic mice not only resulted in severe liver injury with hepatocarcinoma-like histological changes and hepatic AFP production,but also showed an increased serum level of HBV antigens and cytokines like interleukin-6 and tumor necrosis factor-α,compared with the control ***:Over-expression of uPA plays a synergistic role in the development of liver injury,inflammation and regeneration during acute HBV infection.

关键词： Tet-on system Albumin promoter Urokinase-type plasminogen activator Hydrodynamic transfection Liver injury

Enhanced segmentation of gastrointestinal polyps from capsule endoscopy images with artifacts using ensemble learning

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World Journal of Gastroenterology 2022年第41期28卷 5931-5943页

作者： jun-xiao zhou Zhan Yang Ding-Hao Xi Shou-jun Dai Zhi-Qiang Feng jun-Yan Li Wei Xu Hong Wang Department of Gastroenterology and Hepatology Guangzhou First People’s HospitalGuangzhou 510180Guangdong ProvinceChina School of Information Renmin University of ChinaBeijing 100872China

BACKGROUND Endoscopy artifacts are widespread in real capsule endoscopy(CE)images but not in high-quality standard *** To improve the segmentation performance of polyps from CE images with artifacts based on ensemble *** We collected 277 polyp images with CE artifacts from 5760 h of videos from 480 patients at Guangzhou First People’s Hospital from January 2016 to December *** public high-quality standard external datasets were retrieved and used for the comparison *** each dataset,we randomly segmented the data into training,validation,and testing sets for model training,selection,and *** compared the performance of the base models and the ensemble model in segmenting polyps from images with *** The performance of the semantic segmentation model was affected by artifacts in the sample images,which also affected the results of polyp detection by CE using a single *** evaluation based on real datasets with artifacts and standard datasets showed that the ensemble model of all state-of-the-art models performed better than the best corresponding base learner on the real dataset with *** with the corresponding optimal base learners,the intersection over union(IoU)and dice of the ensemble learning model increased to different degrees,ranging from 0.08%to 7.01%and 0.61%to 4.93%,***,in the standard datasets without artifacts,most of the ensemble models were slightly better than the base learner,as demonstrated by the IoU and dice increases ranging from-0.28%to 1.20%and-0.61%to 0.76%,*** Ensemble learning can improve the segmentation accuracy of polyps from CE images with *** results demonstrated an improvement in the detection rate of polyps with interference from artifacts.

关键词： Artifacts Capsule endoscopy Polyps Ensemble learning Segmentation Robustness

Novel biosensor-based microarray assay for detecting rs8099917 and rs12979860 genotypes

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World Journal of Gastroenterology 2012年第44期18卷 6481-6488页

作者： Pei-Yuan Li xiao-jun zhou Lan Yao Xin-Hua Fang Jiang-Nan Ren Jia-Wu Song Department of Gastroenterology the Fifth Affili- ated Hospital of Stm Yat-Sen University Zhuhai 519000 Guang- dong Province China Department of Emergency the Fifth Affiliated Hospital of Sun Yat-Sen University Zhuhai 519000 Guangdong Province China

AIM:To evaluate a novel biosensor-based microarray(BBM) assay for detecting rs12979860 and rs8099917 ***:Four probes specific for rs8099917C/T or rs12979860G/T detection and three sets of quality control probes were designed,constructed and arrayed on an optical biosensor to develop a microarray *** sets of primers were used in a one tube polymerase chain reaction(PCR) system to amplify two target fragments *** biosensor microarray contained probes that had been sequenced to confirm that they included the rs8099917C/T or rs12979860G/T alleles of interest and could serve as the specific assay *** addition to rehybridization of four probes of known sequence,a total of 40 clinical samples collected from hepatitis C seropositive patients were also *** target fragments of all 40 samples were amplified in a 50 μL PCR *** μL of each amplicon was tested by BBM assay,and another 40 μL was used for *** agreement of the results obtained by the two methods was tested statistically using the kappa *** sensitivity of the BBM assay was evaluated using serial dilutions of ten clinical blood samples containing 10 3-10 4 white cells/μ***:As shown by polyacrylamide gel electrophoresis,two target segments of the interleukin 28Bassociated polymorphisms(SNPs) were successfully amplified in the one-tube PCR *** lengths of the two amplified fragments were consistent with the known length of the target sequences,137 and 159 *** hybridization of the PCR amplicons with the probes located on the BBM array,the signals of each allele of both the rs8099917 SNPs and rs12979860 SNPs were observed simultaneously and were clearly visible by the unaided *** signals were distinct from each other,could be interpreted visually,and accurately recorded using an ordinary digital *** evaluate the specificity of the assay,both the plasmids and clinical samples were applied to the ***,30 P

关键词： Biosensor-based microarray Hepatitis C vi-rus rs8099917 rs12979860 Detection Assay

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Polyploid organisms

Science China(Life Sciences) 2012年第4期55卷 301-311页

作者： SONG Can LIU Shaojun xiao jun HE WeiGuo zhou Yi QIN QinBo ZHANG Chun LIU Yun Key Laboratory of Protein Chemistry and Fish Developmental Biology of Ministry of Education of China College of Life Sciences Hunan Normal University Changsha 410081 China

Polyploids are organisms with three or more complete chromosome sets. Polyploidization is widespread in plants and animals, and is an important mechanism of speciation. Genome sequencing and related molecular systematics and bioinformatics studies on plants and animals in recent years support the view that species have been shaped by whole genome duplication during evolution. The stability of polyploids depends on rapid genome recombination and changes in gene expression after formation. The formation of polyploids and subsequent diploidization are important aspects in long-term evolution. Polyploids can be formed in various ways. Among them, hybrid organisms formed by distant hybridization could produce unreduced gametes and thus generate offspring with doubled chromosomes, which is a fast, efficient method of polyploidization. The formation of fertile polyploids not only promoted the interflow of genetic materials among species and enriched the species diversity, but also laid the foundation for polyploidy breeding. The study of polyploids has both important theoretical significance and valuable applications. The production and application of polyploidy breeding have brought remarkable economic and social benefits.

关键词： polyploid whole genome duplication diploidization distant hybridization polyploidy breeding

A fast integral sliding mode controller with an extended state observer for position control of permanent magnet synchronous motor servo systems

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Frontiers of Information Technology & Electronic Engineering 2020年第8期21卷 1239-1250页

作者： jun-feng JIANG xiao-jun zhou Wei ZHAO Wei LI Wen-dong ZHANG State Key Laboratory of Fluid Power and Mechatronic Systems Zhejiang UniversityHangzhou 310027China Zhejiang Province Key Laboratory of Advanced Manufacturing Technology Zhejiang UniversityHangzhou 310027China Northwest Institute of Mechanical and Electrical Engineering Xianyang 712099China Beijing Linkstech Technology Co. LtdBeijing 102200China

Permanent magnet synchronous motor(PMSM)has been widely used in position control *** performance is not satisfactory due to internal uncertainties and external load *** enhance the control performance of PMSM systems,a new method that has fast response and good robustness is proposed in this ***,a modified integral terminal sliding mode controller is developed,which has a fast-sliding surface and a continuous reaching ***,an extended state observer is applied to measure the internal and external ***,the disturbances can be compensated for in a feedforward *** with other sliding mode methods,the proposed method has faster response and better robustness against system *** addition,the position tracking error can converge to zero in a finite *** and experimental results reveal that the proposed control method has fast response and good robustness,and enables high-precision control.

关键词： Permanent magnet synchronous motor(PMSM) Sliding mode controller Extended state observer Robust control Motion control

Paris chinensis dioscin induces G2/M cell cycle arrest and apoptosis in human gastric cancer SGC-7901 cells

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World Journal of Gastroenterology 2011年第39期17卷 4389-4395页

作者： Lin-Lin Gao Fu-Rong Li Peng Jiao Ming-Feng Yang xiao-jun zhou Yan-Hong Si Wen-Jian Jiang Ting-Ting Zheng Department of Pathology and Pathophysiology Taishan Medical University Taian 271000 Shandong Province China School of Pharmaceutical Science Taishan Medical University Taian 271000 Shandong Province China Institute of Basic Medical Sciences Taishan Medical University Taian 271000 Shandong Province China Department of Clinical Medicine Taishan Medical University Taian 271000 Shandong Province China

AIM:To investigate the anti-tumor effects of Paris chinensis dioscin(PCD)and mechanisms regarding cell cycle regulation and apoptosis in human gastric cancer SGC-7901 ***:Cell viability was analyzed by the 3-(4,5-di-methylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide *** apoptosis was evaluated by flow cytometry and laser scanning confocal microscope(LSCM)using Annexin-V/propidium iodide(PI)staining,and the cell cycle was evaluated using PI staining with flow *** calcium ions were detected under fluorescence *** expression of cell cycle and apoptosis-related proteins cyclin B1,CDK1,cytochrome C and caspase-3 was measured by immunohistochemical ***:PCD had an anti-proliferation effect on human gastric cancer SGC-7901 cells in a dose-and time-de-pendent *** treatment of SGC-7901 cells with PCD,apoptosis appeared in SGC-7901 ***-logical changes typical of apoptosis were also observed with LSCM by Annexin V/PI staining,and the cell number of the G0/G1 phase was decreased,while the number of cells in the G2/M phase was *** cycle-related proteins,such as cyclin B1 and CDK1,were all down-regulated,but caspase-3 and cytochrome C were ***,intracellular calcium accumulation occurred in PCD-treated ***:G2/M phase arrest and apoptosis induced by PCD are associated with the inhibition of CDK-activating kinase activity and the activation of Ca2+-related mitochondrion pathway in SGC-7901 cells.

关键词： CyclinB1/CDK1 Cell cycle arrest Caspase-3 Ca2+ Cytochrome C