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Paris chinensis dioscin induces G2/M cell cycle arrest and apoptosis in human gastric cancer SGC-7901 cells

World Journal of Gastroenterology 2011年第39期17卷 4389-4395页

作者： Lin-Lin Gao Fu-Rong Li Peng Jiao Ming-Feng yang xiao-jun Zhou Yan-Hong Si Wen-Jian Jiang Ting-Ting Zheng Department of Pathology and Pathophysiology Taishan Medical University Taian 271000 Shandong Province China School of Pharmaceutical Science Taishan Medical University Taian 271000 Shandong Province China Institute of Basic Medical Sciences Taishan Medical University Taian 271000 Shandong Province China Department of Clinical Medicine Taishan Medical University Taian 271000 Shandong Province China

AIM:To investigate the anti-tumor effects of Paris chinensis dioscin(PCD)and mechanisms regarding cell cycle regulation and apoptosis in human gastric cancer SGC-7901 ***:Cell viability was analyzed by the 3-(4,5-di-methylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide *** apoptosis was evaluated by flow cytometry and laser scanning confocal microscope(LSCM)using Annexin-V/propidium iodide(PI)staining,and the cell cycle was evaluated using PI staining with flow *** calcium ions were detected under fluorescence *** expression of cell cycle and apoptosis-related proteins cyclin B1,CDK1,cytochrome C and caspase-3 was measured by immunohistochemical ***:PCD had an anti-proliferation effect on human gastric cancer SGC-7901 cells in a dose-and time-de-pendent *** treatment of SGC-7901 cells with PCD,apoptosis appeared in SGC-7901 ***-logical changes typical of apoptosis were also observed with LSCM by Annexin V/PI staining,and the cell number of the G0/G1 phase was decreased,while the number of cells in the G2/M phase was *** cycle-related proteins,such as cyclin B1 and CDK1,were all down-regulated,but caspase-3 and cytochrome C were ***,intracellular calcium accumulation occurred in PCD-treated ***:G2/M phase arrest and apoptosis induced by PCD are associated with the inhibition of CDK-activating kinase activity and the activation of Ca2+-related mitochondrion pathway in SGC-7901 cells.

关键词： CyclinB1/CDK1 Cell cycle arrest Caspase-3 Ca2+ Cytochrome C

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行政处罚快速办理程序的正当性审视与补强

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行政法学研究 2022年第2期 30-43页

作者：杨小军毛晨宇中共中央党校(国家行政学院)政法部北京100091

随着“放管服”改革的持续推进,行政程序的程序效能大幅提升,行政处罚快速办理程序也随之出现。然而快速办理程序在实现程序加速的同时也带来了正当性的风险,一方面其适用范围与证明标准可能影响程序的工具正当性;另一方面其对行政方式... 详细信息

随着“放管服”改革的持续推进,行政程序的程序效能大幅提升,行政处罚快速办理程序也随之出现。然而快速办理程序在实现程序加速的同时也带来了正当性的风险,一方面其适用范围与证明标准可能影响程序的工具正当性;另一方面其对行政方式的转变、启动机制的设计以及规范性文件公开的不足可能影响其内在正当性。为补强快速办理程序的正当性,其应在适用范围、行政方式、证明标准、程序动议、权利义务告知、抽检监督等六个方面作出改进。

关键词：行政程序行政处罚快速办理程序正当程序行政效能

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Original article Preliminary analysis of cerebrospinal fluid proteome in patients with neurocysticercosis

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Chinese Medical Journal 2009年第9期122卷 1003-1008页

作者： TIAN xiao-jun LI Jing-yi HUANG Yong XUE Yan-ping Beijing Tropical Medicine Research Institute Beijing FriendshipHospital Capital Medical University Beijing 100050 China Shandong Institute of Parasitic Diseases Jining Shandong 272033China

Background Neurocysticercosis is the infection of the nervous system by the larvae of Taenia solium （T. solium）. Despite continuous effort, the experimental diagnosis of neurocysticercosis remains unresolved. Since the cerebrospinal fluid （CSF） contacts with the brain, dynamic information about pathological processes of the brain is likely to be reflected in CSF. Therefore, CSF may serve as a rich source of putative biomarkers related to neurocysticercosis. Comparative proteomic analysis of CSF of neurocysticercosis patients and control subjects may find differentially expressed proteins.Methods Two-dimensional difference in gel electrophoresis （2D-DIGE） was used to investigate differentially expressed proteins in CSF of patients with neurocysticercosis by comparing the protein profile of CSF from neurocysticercosis patients with that from control subjects. The differentially expressed spots/proteins were recognized with matrix-assisted laser desorption/ionization-time of flight-time of flight （MALDI-TOF-TOF） mass spectrometry.Results Forty-four enzyme digested peptides were obtained from 4 neurocysticercotic patients. Twenty-three were identified through search of the NCBI protein database with Mascot software, showing 19 up-expressed and 4 down-expressed. Of these proteins, 26S proteosome related to ATP- and ubiquitin-dependent degradation of proteins and lipocalin type prostaglandin D synthase involved in PGD2-synthesis and extracellular transporter activities were up-expressed, while transferrin related to iron metabolism within the brain was down-expressed.Conclusions This study established the proteomic profile of pooled CSF from 4 patients with neurocysticercosis, suggesting the potential value of proteomic analysis for the study of candidate biomarkers involved in the diagnosis or pathogenesis of neurocysticercosis.

关键词： neurocysticercosis cerebrospinal fluid two-dimensional difference in-gel electrophoresis oroteomic analysis

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Treatment of infant postrenal acute renal failure followingobstruction due to upper urinary calculi

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Frontiers of Medicine 2010年第1期4卷 127-130页

作者： Hui-Xia ZHOU Zhi-Chun FENG Hao MENG xiao-Guang ZHOU Shuang LI jun WANG Shi-Xi DAI Department of Urology Bayi Children’s HospitalGeneral Hospital of Beijing Military RegionBeijing 100700China Department of Urology Wuhan Children’s HospitalWuhan 430030China

The surgical emergency treatments and cura-tive effects of postrenal acute renal failure following obstruction due to upper urinary calculi in infants were *** the 13 infants with postrenal acute renal failure following obstruction due to upper urinary calculi,11 received retrograded catheterizations of the ureter with semi-rigid ureteroscopy(F 6.8),and two received open *** results showed that only one infant had anuresis and continuous reduction of hemoglobin 5 h after the open ureterolithotomy and received exploration via excision and peritoneal dialysis,and the remaining 12 patients well recovered in this *** renal function of all the patients was restored without postoperative *** is concluded that the retrograded catheterization of the ureter with ureteroscopy is a minimally invasive,safe and effective therapy for postrenal acute renal failure following obstruction due to upper urinary calculi in *** those infants whose urethras are thin and small,the open ureterolithotomy is a suitable *** patients with bleeding tendency need to be corrected prior to the open ureterotomy to remove obstructions.

关键词： infant acute renal failure postrenal uretero-scopy

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模拟自由面影响的涡格方法

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水动力学研究与进展（A辑） 2020年第3期35卷 321-327页

作者：陈强董小倩杨晨俊上海交通大学海洋工程国家重点实验室上海200240

基于涡格法来计算自由面影响下的水下航行体或螺旋桨水动力性能。水下航行体、螺旋桨以及自由面均采用涡格来表达,航行体涡格布置在真实物面上,桨叶涡格布置在拱弧面上,自由面涡格布置在静水面上方合适的距离处。通过奇点系的诱导速度... 详细信息

基于涡格法来计算自由面影响下的水下航行体或螺旋桨水动力性能。水下航行体、螺旋桨以及自由面均采用涡格来表达,航行体涡格布置在真实物面上,桨叶涡格布置在拱弧面上,自由面涡格布置在静水面上方合适的距离处。通过奇点系的诱导速度来模拟物体与自由面的相互作用,在物面(或螺旋桨拱弧面)上满足不可穿透条件及在静水面上满足线性自由面条件,建立关于涡强的线性方程组并联立求解。通过球体、水翼及螺旋桨等算例考察了该方法的收敛性和精度,并讨论了进速和沉深对螺旋桨水动力性能的影响。

关键词：涡格法自由面球体水翼螺旋桨

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德国跨国高等教育:动因、模式与特征

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比较教育研究 2018年第8期40卷 91-98页

作者：肖军北京师范大学国际与比较教育研究院北京100875

近年来,德国跨国高等教育在联邦教育与科研部资助、德意志学术交流中心主导下快速发展。德国跨国高等教育可以分为与国外高校联合开设专业、建立德国高校支持的本土高校、设立大学海外分校或研究所、设立德资海外独立高校四种模式。德... 详细信息

近年来,德国跨国高等教育在联邦教育与科研部资助、德意志学术交流中心主导下快速发展。德国跨国高等教育可以分为与国外高校联合开设专业、建立德国高校支持的本土高校、设立大学海外分校或研究所、设立德资海外独立高校四种模式。德国跨国高等教育的发展有着不同于英美澳等国的特点,更具有国家战略色彩、专业设置以工科和自然科学专业为主、教学和科研相结合。在新的国际环境下,德国跨国高等教育也面临激烈的市场竞争,在项目运行中出现了一些问题。

关键词：德国跨国高等教育境外办学

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Two-channel, dual-beam-mode, wavelength-tunable femtosecond optical parametric oscillator

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Advanced Photonics 2020年第4期2卷 24-28页

作者： Jintao Fan jun Zhao Liping Shi Na xiao Minglie Hu Tianjin University College of Precision Instrument and Optoelectronics EngineeringUltrafast Laser LaboratoryTianjinChina Leibniz Universität Hannover Institut für QuantenoptikHannoverGermany Cluster of Excellence PhoenixD(Photonics Opticsand Engineering-Innovation Across Disciplines)HannoverGermany

Optical vortices,which carry orbital angular momentum,offer special capabilities in a host of applications.A single-laser source with dual-beam-mode output may open up new research fields of nonlinear optics and quantum *** demonstrate a dual-channel scheme to generate femtosecond,dualwavelength,and dual-beam-mode tunable signals in the near infrared wavelength ***-wavelength operation is derived by stimulating two adjacent periods of a periodically poled lithium niobate *** by an Yb-doped fiber laser with a Gaussian(lp?0)beam,two tunable signal emissions with different beam modes are observed *** one of the emissions can be tuned from1520 to 1613 nm with the Gaussian(ls?0)beam,the other is capable of producing a vortex spatial profile with different vortex orders(ls?0 to 2)tunable from 1490 to 1549 *** proposed system provides unprecedented freedom and will be an exciting platform for super-resolution imaging,nonlinear optics,multidimensional quantum entanglement,etc.

关键词： nonlinear optics parametric processes optical parametric oscillators ultrafast nonlinear optics

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基于导弹舵面信息的惯导姿态角速率滤波方法

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中国惯性技术学报 2011年第3期19卷 281-285页

作者：卢晓东周军刘小军西北工业大学精确制导与控制研究所西安710072

针对导弹武器中低精度惯导系统陀螺所测量的弹体姿态角速率误差较大的问题,提出了利用导弹控制舵面信息与弹体姿态角的动力学关系构建滤波方程的方法,其相对于常用的低通滤波器能够更加精确地反映出姿态角的变化趋势。此外为降低采用EKF... 详细信息

针对导弹武器中低精度惯导系统陀螺所测量的弹体姿态角速率误差较大的问题,提出了利用导弹控制舵面信息与弹体姿态角的动力学关系构建滤波方程的方法,其相对于常用的低通滤波器能够更加精确地反映出姿态角的变化趋势。此外为降低采用EKF和UKF等非线性滤波方法所带来的计算复杂度,仿真中通过构造交错卡尔曼滤波器实现了对非线性系统的伪线性化。最后通过对某型低精度惯组在三轴姿态转台上的半实物仿真实验,该滤波算法将实测的惯导姿态角速率误差降低了40%以上。

关键词：姿态角速率舵面信息交错卡尔曼滤波低精度惯导

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Orange-derived extracellular vesicles nanodrugs for efficient treatment of ovarian cancer assisted by transcytosis effect

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Acta Pharmaceutica Sinica B 2023年第12期13卷 5121-5134页

作者： Feng Long Yao Pan Jinheng Li Suinan Sha Xiubo Shi Haoyan Guo Chuanqing Huang Qian xiao Chao Fan Xingmei Zhang jun-Bing Fan Ying Wang Cancer Research Institute Experimental Education/Administration CenterKey Laboratory of Functional Proteomics of Guangdong ProvinceSchool of Basic Medical SciencesSouthern Medical UniversityGuangzhou 510515China Department of Neurobiology School of Basic Medical SciencesSouthern Medical UniversityGuangzhou 510515China

Extracellular vesicles(EVs)have recently received much attention about the application of drug carriers due to their desirable properties such as nano-size,biocompatibility,and high ***,we demonstrate orange-derived extracellular vesicles(OEV)nanodrugs(DN@OEV)by modifying cRGD-targeted doxorubicin(DOX)nanoparticles(DN)onto the surface of OEV,enabling significantly enhancing tumor accumulation and penetration,thereby efficiently inhibiting the growth of ovarian *** obtained DN@OEV enabled to inducement of greater transcytosis capability in ovarian cancer cells,which presented the average above 10-fold transcytosis effect compared with individual *** was found that DN@OEV could trigger receptor-mediated endocytosis to promote early endosome/recycling endosomes pathway for exocytosis and simultaneously reduce degradation in the early endosomes-late endosomes-lysosome pathway,thereby inducing the enhanced *** particular,the zombie mouse model bearing orthotopic ovarian cancer further validated DN@OEV presented high accumulation and penetration in tumor tissue by the transcytosis *** study indicated the strategy in enhancing transcytosis has significant implications for improving the therapeutic efficacy of thedrugdelivery system.

关键词： Extracellular vesicles Transcytosis Cancer treatment Drug delivery system Targeted modification Receptor-mediated endocytosis Intracellular trafficking Recycling endosome

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Improvement of human embryonic stem cell-derived retinal pigment epithelium cell adhesion, maturation, and function through coating with truncated recombinant human vitronectin

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International Journal of Ophthalmology(English edition) 2021年第8期14卷 1160-1167页

作者： Xin-Yue Zhu Yu-Hong Chen Ting Zhang Su-jun Liu Xin-Yue Bai Xian-Yu Huang Mei Jiang xiao-Dong Sun Department of Ophthalmology Shanghai General HospitalShanghai Jiao Tong University School of MedicineShanghai 200080China National Clinical Research Center for Eye Diseases Shanghai 200080China Shanghai Key Laboratory of Fundus Diseases Shanghai 200080China Shanghai Engineering Center for Visual Science and Photomedicine Shanghai 200080China

AIM:To explore an xeno-free and defined coating substrate suitable for the culture of H9 human embryonic stem cell-derived retinal pigment epithelial(hES-RPE)cells in vitro,and compare the behaviors and functions of h ESRPE cells on two culture substrates,laminin521(LN-521)and truncated recombinant human vitronectin(VTN-N).METHODS:hES-RPE cells were used in the *** abilities of LN-521 and VTN-N at different concentrations to adhere to hES-RPE cells were compared with a high-content imaging *** real-time polymerase chain reaction was used to evaluate RPE-specific gene expression levels midway(day 10)and at the end(day 20)of the time *** polarity was observed by immunofluorescent staining for apical and basal markers of the *** phagocytic ability of hES-RPE cells was identified by flow cytometry and ***:The cell adhesion assay showed that the ability of LN-521 to adhere to hES-RPE cells was *** increasing coating concentration,an increasing number of cells attached to the surface of LN-521-coated *** contrast,VTN-N presented a strong adhesive ability even at a low *** optimal concentration of LN-521 and VTN-N required to coat and adhesion to hES-RPE cells were 2 and 0.25μg/cm^(2),***,both LN-521 and VTN-N could facilitate adoption of the desired cobblestone cellular morphology with tight junction and showed polarity by the hES-RPE ***,hES-RPE cells cultivated in VTN-N had a greater phagocytic ability,and it took less time for these hES-RPE cells to ***:VTN-N is a more suitable coating substrate for cultivating hES-RPE cells.

关键词： retinal pigment epithelial stem cell surface coating laminin vitronectin cell adhesion phagocytosis

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