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Comparative transcriptomic analysis reveals the molecular changes of acute pancreatitis in experimental models

World Journal of Gastroenterology 2024年第14期30卷 2038-2058页

作者： pan Zheng Xue-Yang Li xiao-Yu Yang huan Wang Ling Ding Cong He Jian-hua Wan hua-Jing Ke Nong-hua Lu Nian-Shuang Li Yin Zhu Department of Gastroenterology Digestive Disease HospitalThe First Affiliated HospitalJiangxi Medical CollegeNanchang UniversityNanchang 330006Jiangxi ProvinceChina

BACKGROUND Acute pancreatitis(Ap)encompasses a spectrum of pancreatic inflammatory conditions,ranging from mild inflammation to severe pancreatic necrosis and multisystem organ *** the challenges associated with obtaining human pancreatic samples,research on Ap predominantly relies on animal *** this study,we aimed to elucidate the fundamental molecular mechanisms underlying Ap using various Ap *** To investigate the shared molecular changes underlying the development of Ap across varying severity *** Ap was induced in animal models through treatment with caerulein alone or in combination with lipopolysaccharide(LpS).Additionally,using ptf1αto drive the specific expression of the hM3 promoter in pancreatic acinar cells transgenic C57BL/6J-hM3/ptf1α(cre)mice were administered Clozapine N-oxide to induce ***,we conducted RNA sequencing of pancreatic tissues and validated the expression of significantly different genes using the Gene Expression Omnibus(GEO)*** Caerulein-induced Ap showed severe inflammation and edema,which were exacerbated when combined with LpS and accompanied by partial pancreatic tissue *** with the control group,RNA sequencing analysis revealed 880 significantly differentially expressed genes in the caerulein model and 885 in the caerulein combined with the LpS *** Encyclopedia of Genes and Genomes enrichment analysis and Gene Set Enrichment Analysis indicated substantial enrichment of the TLR and NOD-like receptor signaling pathway,TLR signaling pathway,and NF-κB signaling pathway,alongside elevated levels of apoptosis-related pathways,such as apoptosis,p53 pathway,and phagosome *** significantly elevated genes in the TLR and NOD-like receptor signaling pathways,as well as in the apoptosis pathway,were validated through quantitative real-time pCR experiments in animal *** from the GEO database revealed that only MYD88 concurred in both mouse panc

关键词： Acute pancreatitis RNA-sequencing Experimental acute pancreatitis models inflammatory Apoptosis TLR and NOD-like signaling pathways

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Mechanism of high-energy pulsed current-assisted rolling of 08AL carbon steel ultra-thin strip

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Journal of iron and Steel Research international 2024年第2期31卷 416-427页

作者： Zhong-kai Ren Ya-nan Xu Jia-zhao Chen He Li Rui-lin Yuan Zhen-hua Wang Tao Wang xiao Liu College of Mechanical and Vehicle Engineering Taiyuan University of TechnologyTaiyuan030024ShanxiChina Engineering Research Center of Advanced Metal Composites Forming Technology and Equipment(Ministry of Education) Taiyuan University of TechnologyTaiyuan030024ShanxiChina National Key Laboratory of Metal Forming Technology and Heavy Equipment China National Heavy Machinery Research Institute Co.Ltd.Xi’an710016ShaanxiChina

To improve the plastic deformation performance of a 08AL carbon steel ultra-thin strip,a pulsed electric field was integrated into the plastic processing of the ultra-thin strip,and the effects of high-energy current on its deformation ability were ***-assisted tensile tests were employed,and the results clarified that the pulsed current could reduce the activation energy of faults and promoted dislocation slip within grains and at grain boundaries,leading to a decrease in the deformation resistance of the metal and an increase in its plastic *** the current density of 2.0 A/mm2,the yield strength,tensile strength,and elongation of the rolled sample reached 425 Mpa,467 Mpa,and 12.5%,*** the rolling process,it was found that the pulsed current promoted the dynamic recrystallization of the ultra-thin strip,reduced its dislocation density and deformation resistance,and promoted the coordinated deformation of the metal.

关键词： 08AL ultra-thin strip High-energy pulsed current Mechanical property Dislocation density Dynamic recrystallization

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Necroptosis contributes to non-alcoholic fatty liver disease pathoetiology with promising diagnostic and therapeutic functions

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World Journal of Gastroenterology 2024年第14期30卷 1968-1981页

作者： Hong-Ju Sun Bo Jiao Yan Wang Yue-hua Zhang Ge Chen Zi-Xuan Wang Hong Zhao Qing Xie xiao-hua Song Department of General Medicine Qingdao Central HospitalUniversity of Health and Rehabilitation Sciences(Qingdao Central Medical Group)Qingdao 266042Shandong ProvinceChina Department of Gastroenterology Qingdao Central HospitalUniversity of Health and Rehabilitation Sciences(Qingdao Central Medical Group)Qingdao 266042Shandong ProvinceChina Department of Medical Administration Qingdao Central HospitalUniversity of Health and Rehabilitation Sciences(Qingdao Central Medical Group)Qingdao 266042Shandong ProvinceChina Qingdao Medical College Qingdao UniversityQingdao 266042Shandong ProvinceChina Department of infectious Diseases Ruijin HospitalShanghai Jiao Tong University School of MedicineShanghai 200025China

Nonalcoholic fatty liver disease(NAFLD)is the most prevalent type of chronic liver ***,the disease is underappreciated as a remarkable chronic disorder as there are rare managing *** studies have focused on determining NAFLD-caused hepatocyte death to elucidate the disease pathoe-tiology and suggest functional therapeutic and diagnostic ***,ferroptosis,and necroptosis are the main subtypes of non-apoptotic regulated cell deaths(RCDs),each of which represents particular *** the complexity of the findings,the present study aimed to review these types of RCDs and their contribution to NAFLD progression,and subsequently discuss in detail the role of necroptosis in the pathoetiology,diagnosis,and treatment of the *** study revealed that necroptosis is involved in the occurrence of NAFLD and its progression towards steatohepatitis and cancer,hence it has potential in diagnostic and therapeutic ***,further studies are necessary.

关键词： Nonalcoholic fatty liver disease Apoptosis Necroptosis Cell death Diagnosis Treatment

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Fluoro-functionalized plant biomass adsorbent:preparation and app.ication in extraction of trace perfluorinated compounds from environmental water samples

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Journal of Environmental Sciences 2024年第3期137卷 703-715页

作者： Xi Tan Yan Shi Chun-Feng Ma Quan Chi Yu-Hang Yang Wen-Xiang Zhang hua-Ming xiao Xian Wang Key Laboratory of Analytical Chemistry of the State Ethnic Affairs Commission School of Chemistry and Materials ScienceSouth-Central Minzu UniversityWuhan 430074China School of pharmaceutical Sciences South-Central Minzu UniversityWuhan 430074China Key Laboratory of Oilseeds processing of Ministry of Agriculture Hubei Key Laboratory of Lipid Chemistry and NutritionOil Crops Research Institute of the Chinese Academy of Agricultural SciencesWuhan 430062China

perfuorinated compounds(pFCs)are toxic and widely present in the environment,and therefore effective adsorbents are required to remove pFCs from environmental *** the present study,a new type of fuorinated biomass materials was synthesized via an ingenious fuorosilanization *** adsorbents were app.ied for the adsorp.ion of 13typical pFCs,including perfuorocarboxylic acids(pFCAs)and perfuorosulfonic acids(pFSAs).By comparing their adsorp.ion performance,Fluorinated cedar slag(FCS)was discovered to have the best absorp.ion efficiency and enabled highly efficient enrichment of *** adsorp.ion recovery of FCS with the investigated pFCs is greater than 90%under the op.imal adsorp.ion *** to the high affinity of F-F sorbent-sorbate interaction,FCS had good adsorp.ion capacities of pFCs from aqueous solution,with the maximum adsorp.ion capacity of 15.80 mg/g for pFOS and 10.71 mg/g for pFOA,***,the adsorp.ion time could be achieved in a short time(8 min).Using the FCS absorbent,an innovative FCS-solid phase extraction assisted with high performance liquid chromatography-electrospray-tandem mass spectrometry(FCS-SpE-HpLC-ESi-MS/MS)method was first developed to sensitively detect pFCs in the environmental water *** intra-day and inter-day recovery rates of the 13 compounds ranged from 90.7%-104.3%,with the RSD of 2.1%-4.7%(intra-day)and 2.5%-8.5%(inter-day),*** research demonstrates the potential of the newly fuoro-functionalized plant biomass to adsorb pFCs from environmental water,with the advantages of high adsorp.ion efficiencies,high antiinterference,easy operation and low economic cost.

关键词： Fluoro-functionalized biomass perfuorinated compounds(pFCs) Adsorp.ion efficiency Mass spectrometry(MS) Fluorinated cedar slag(FCS)

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Brazilin Actuates Ferroptosis in Breast Cancer Cells via p53/SLC7A11/GpX4 Signaling pathway

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Chinese Journal of integrative Medicine 2024年第11期30卷 1001-1006页

作者： HE Dan TAN xiao-ning Li Lin-p.i GAO Wen-hui TiAN Xue-fei ZENG pu-hua Hunan Academy of Chinese Medicine School of integrated Chinese and Western Medicine Hunan University of Chinese Medicine Scientific Research Department The Affiliated Hospital of Hunan Academy of Traditional Chinese Medicine Oncology Department The Affiliated Hospital of Hunan Academy of Traditional Chinese Medicine School of Traditional Chinese Medicine Hunan University of Chinese Medicine

Objective:To investigate the mechanism of induction of ferroptosis by brazilin in breast cancer ***:Breast cancer 4T1 cells were divided into 6 groups:control,brazilin 1/2 half maximal inhibitory concentration(iC50),iC50,2×iC50,erastin（10 μg/mL） and capecitabine（10 μg/mL） *** effect of brazilin on the proliferation of 4T1 cells was detected by cell counting kit-8 assay,and the treatment dose of brazilin was *** effect of brazilin on the mitochondrial morphology of 4T1 cells,and the mitochondrial damage was evaluated under electron *** levels of Fe2+,reactive oxygen species（ROS）,malondialdehyde（MDA）,glutathione（GSH） and glutathione peroxidase 4（GpX4） were estimated using various detection *** invasion and migration abilities of 4T1 cells were detected by scratch assay and transwell *** expressions levels of tumor protein p53, solute carrier family 7 member 11（SLC7A11）,GpX4 and acyl-CoA synthetase long-chain family member 4（ACSL4） proteins were quantified by Western blot ***:Compared to the control group,the 10(1/2 iC50),20(iC50) and 40(2×iC50) μg/mL brazilin,erastin,and capecitabine groups showed a significant decrease in the cell survival rate,invasion and migration abilities,GSH,SLC7A11 and GpX4 protein expression levels,and mitochondrial volume and ridge（pignificant increase in the mitochondria membrane density,Fe2+,ROS and MDA levels,and p53 and ACSL4protein expression levels（pions:Brazilin actuated ferroptosis in breast cancer cells,and the underlying mechanism is mainly associated with the p53/SLC7A11/GpX4 signaling pathway.

关键词： Chinese medicine brazilin breast cancer ferroptosis p53/SLC7A11/GpX4 axis

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Neomicrocoleus ***.(Microcoleaceae,Oscillatoriales),a novel cyanobacterial genus from benthic mats in a water channel

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Journal of Oceanology and Limnology 2024年第1期42卷 263-276页

作者： Ruozhen GENG Zhongshi HE Kaihui GAO peng xiao He ZHANG Si CHEN hua Li Gongliang YU Renhui Li College of Life and Environmental Sciences Wenzhou UniversityWenzhou 325035China Key Laboratory of Algal Biology Institute of HydrobiologyChinese Academy of SciencesWuhan 430072China University of Chinese Academy of Sciences Beijing 100049China Hubei institution of Land Surveying and Map.ing Wuhan 430072China

A new filamentous cyanobacterial strain(CHAB 4127)was successfully isolated from the algal mat during the field investigation of the open channel from Luanhe River to Tianjin,*** polyphasic approach combining morphological,ultrastructural,ecological,and molecular features was used to characterize this studied *** strain is morphologically similar to the Microcoleus-like cyanobacterial taxa under light microscopy,and the radial arrangement of thylakoids is also consistent with that of Microcoleus-like *** phylogenetic position of CHAB 4127 based on 16S rRNA gene sequences,is shown to be clearly clustered into an independent clade with the newly established genus *** maximum similarity of 16S r RNA gene of the studied strain with other existing related cyanobacterial taxa is 93.97%,and the iTS secondary structures is also obviously different from other members of *** on all the above evidence,we proposed the establishment of this novel cyanobacterial genus as Neomicrocoleus,with its type species,Neomicrocoleus tianjinensis.

关键词： Neomicrocoleus tianjinensis morphology 16S rRNA taxonomy polyphasic approach

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Barley protein LFBEp-C1 from Lactiplantibacillus plantarum dy-1 Fermented Barley Extracts by inhibiting Lipid Accumulation in a Caenorhabditis elegans Model

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Biomedical and Environmental Sciences 2024年第4期37卷 377-386页

作者： ZHANG Jia Yan LiU Meng Ting LiU Yu Hao DENG huan BAi Juan XiE Jian hua xiao Xiang School of Food and Biological Engineering Jiangsu UniversityZhenjiang 212013JiangsuChina State Key Laboratory of Food Science and Technology Nanchang UniversityNanchang 330047JiangxiChina

Objective This study aimed to investigate the lipid-lowering activity of LFBEp-C1 in high glucose-fed Caenorhabditis elegans(***).Methods in this study,the fermented barley protein LFBEp-C1 was prepared and tested for its potential anti-obesity effects on *** worms were fed Escherichia coli Op50(*** Op50),glucose,and different concentrations of *** size,lifespan,movement,triglyceride content,and gene expression were *** results were analyzed using ANOVA and Tukey's multiple comparison *** Compared with the model group,the head-swing frequency of *** in the group of LFBEp-C1 at 20μg/mL increased by 33.88%,and the body-bending frequency increased by 27.09%.This indicated that LFBEp-C1 improved the locomotive ability of *** average lifespan of *** reached 13.55 days,and the body length and width of the *** decreased after LFBEp-C1 ***,LFBEp-C1 reduced the content of lipid accumulation and triglyceride *** expression levels of sbp-1,daf-2,and mdt-15 significantly decreased,while those of daf-16,tph-1,mod-1,and ser-4 significantly increased after LFBEp-C1 *** in these genes explain the signaling pathways that regulate lipid *** LFBEp-C1 significantly reduced lipid deposition in *** fed a high-glucose diet and alleviated the adverse effects of a high-glucose diet on the development,lifespan,and exercise behavior of *** addition,LFBEp-C1 regulated lipid metabolism mainly by mediating the expression of genes in the sterol regulatory element-binding protein,insulin,and 5-hydroxytryptamine signaling pathways.

关键词： LFBEp-C1 Fermentation protein Caenorhabditis elegans Lipid accumulation Signaling pathway

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proteomic profiling of cerebrospinal fluid in pediatric myelin oligodendrocyte glycoprotein antibody-associated disease

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World Journal of pediatrics 2024年第3期20卷 259-271页

作者： Yi-Long Wang Meng-Ying Zhu Zhe-Feng Yuan xiao-Yan Ren xiao-Tong Guo Yi hua Lu Xu Cong-Ying Zhao Li-hua Jiang Xin Zhang Guo-Xia Sheng p.i-Fang Jiang Zheng-Yan Zhao Feng Gao Department of Neurology Children's HospitalZhejiang University School of MedicineHangzhou 310052China Children's Hospital Zhejiang University School of MedicineHangzhou 310052China Children's Hospital National Clinical Research Center for Child HealthZhejiang University School of MedicineHangzhou 310052China

Background Myelin oligodendrocyte glycoprotein(MOG)antibody-associated disease(MOGAD)is an autoimmune demy-elinating disorder of the central nervous *** Extracted proteins from 34 cerebrospinal fluid(CSF)samples[patients with MOGAD(MOG group,n=12);healthy controls(HC group,n=12);patients with MOG seronegative and metagenomics next-generation sequencing-negative inflammatory neurological diseases(iND group,n=10)]were processed and subjected to label-free quantitative *** partial least squares-discriminant analysis(pLS-DA)and orthogonal pLS-DA(O-pLS-DA)models were also performed based on proteomics *** analysis of differentially expressed proteins(DEps)was performed using Gene Ontology,interpro,and Kyoto Encyclopedia Genes and *** enzyme-linked immunosorbent assay was used to determine the complement levels in serum from patients with *** Four hundred and twenty-nine DEps(149 upregulated and 280 downregulated proteins)were identified in the MOG group compared to the HC group according to the p value and fold change(FC).Using the O-pLS-DA model,872 differentially abundant proteins were identified with variable importance projection(Vip)scores>*** proteins(gamma-glutamyl hydrolase,cathep.in F,interalpha-tryp.in inhibitor heavy chain 5,latent transforming growth factor beta-binding protein 4 and leukocyte-associated immunoglobulin-like receptor 1)overlap.ing between the top 30 DEps with top-ranked p value and FC and top 30 proteins in pLS-DA Vip lists were *** analysis revealed that the dysregulated proteins in the MOG group were p.imarily involved in complement and coagulation cascades,cell adhesion,axon guidance,and glycosp.ingolipid biosynthesis compared to the HC *** The proteomic alterations in CSF samples from children with MOGAD identified in the current study might provide opportunities for developing novel biomarker candidates.

关键词： Acute disseminated encephalomyelitis Cerebrospinal fluid Complement cascades Myelin oligodendrocyte glycoprotein-associated disease proteomics

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Comparison of biliary protein spectrum in gallstone patients with obesity and those with normal body weight

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Hepatobiliary & pancreatic Diseases international 2024年第4期23卷 385-392页

作者： Min-Zhi Chen ping Xie xiao-Chang Wu Zhen-hua Tan Hai Qian Zhi-Hong Ma Xing Yao Department of Hepatopancreatobiliary Surgery Huzhou Central HospitalAffiliated Huzhou HospitalZhejiang University School of MedicineHuzhou 313000China Huzhou Key Laboratory of Molecular Medicine Huzhou Central HospitalAffiliated Huzhou HospitalZhejiang University School of MedicineHuzhou 313000China

Background: Obesity is a common public health issue and is currently deemed a disease. Research has shown that the risk of gallstones in individuals with obesity is elevated. This study aimed to explore the bile proteomics differences between cholelithiasis patients with obesity and normal body weight. Methods: Bile samples from 20 patients(10 with obesity and 10 with normal body weight) who underwent laparoscopic cholecystectomy at our center were subjected to tandem mass tag labeling(TMT) and liquid chromatography-tandem mass spectrometry(LC-MS/MS), followed by further bioinformatic analysis. Results: Among the differentially expressed proteins, 23 were upregulated and 67 were downregulated. Bioinformatic analysis indicated that these differentially expressed proteins were mainly involved in cell development, inflammatory responses, glycerolipid metabolic processes, and protein activation cascades. in addition, the activity of the peroxisome proliferator-activated receptor(ppAR, a subfamily of nuclear receptors) signaling pathway was decreased in the Kyoto Encyclopedia of Genes and Genomes(KEGG) enrichment analysis. Two downregulated proteins in the ppAR signaling pathway, ApO A-Ⅰ and ApO A-Ⅱ, were confirmed using enzyme-linked immunosorbent assay. Conclusions: The ppAR signaling pathway may play a crucial role in the development of cholelithiasis among patients with obesity. Furthermore, biliary proteomics profiling of gallstones patients with obesity is revealed, providing a reference for future research.

关键词： proteome profiling Gallstones Obesity-associated gallstones Tandem mass tag labeling ppAR signaling

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investigating the Mechanism of Qu Du Qiang Fei 1 Hao Fang Formula against Coronavirus Disease 2019 Based on Network pharmacology Method

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World Journal of Traditional Chinese Medicine 2024年第1期10卷 93-103页

作者： Yuan-hua Wang He-Yang Zhou Jin-Yun Ma Gui-Qing Ding hua Yu Yong-Sheng Jin xiao-Dong Cheng institute of Clinical immunology Yue-Yang Hospital of Integrative MedicineShanghai University of Traditional Chinese MedicineShanghaiChina School of pharmacy Naval Medical UniversityShanghaiChina

Objective:Qu Du Qiang Fei 1 Hao Fang(QDQF1)is a novel Chinese herbal medicine formula used to treat coronavirus disease 2019(COViD-19).However,the pharmacological mechanisms of action of QDQF1 remain *** objective of this study was to identify the effective ingredients and biological targets of QDQF1 for COViD-19 *** and Methods:The effective ingredients and mechanisms of action of QDQF1 were analyzed by using network pharmacology methods,which included an analysis of the effective ingredients and corresponding targets,COViD-19-related target acquisition,compound-target network analyses,protein-protein interaction network analysis,Kyoto Encyclopedia of Genes and Genomes(KEGG)and Gene Ontology(GO)enrichment analyses,and molecular docking ***:in total,288 effective QDQF1 ingredients were *** identified 51 core targets from the 148 targets through an overlap between putative QDQF1 targets and COViD-19-related *** key components,including formononetin,kaempferol,luteolin,naringenin,quercetin,and wogonin were identified through component-target network *** functional enrichment analysis of the core targets revealed 1296 items,while KEGG pathway enrichment analysis identified 148 signaling *** central targets(CCL2,CXCL8,iL1B,iL6,MApK1,MApK3,MApK8,STAT3,and TNF)related to the COViD-19 pathway were identified in the KEGG pathway enrichment ***,molecular docking analysis suggested that the docking scores of the six key components to the nine central targets were better than those to ***:QDQF1 may regulate multiple immune-and inflammation-related targets to inhibit the progression of severe acute respiratory syndrome coronavirus 2,and thus,may be suitable for the treatment of COViD-19.

关键词： Coronavirus disease 2019 molecular docking molecular mechanism network pharmacology Qu Du Qiang Fei 1 Hao Fang formula

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