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Various infection status and molecular evidence for horizontal transmission and recombination of Wolbachia and Cardinium among rice planthoppers and related species

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Insect Science 2013年第3期20卷 329-344页

作者： Kai-Jun Zhang xiao han xiao-Yue Hong Department of Entomology Nanjing Agricultural University Nanjing China

Wolbachia and Cardinium are widely distributed and are considered important for their ability to disturb reproduction and affect other fitness-related traits of their hosts. By using multilocus sequence typing （MLST）, RFLP （restriction fragment length poly- morphism） and 16S ribosomal DNA gene sequencing methods, we extensively surveyed Wolbachia and Cardinium infection status of four predominant rice planthoppers and one kind of leafhopper in different rice fields. The results demonstrated that Sogatellafurcifera （Horv^ith） and Laodelphax striatellus （Fallrn） were infected with the same Wolbachia strain （wStri）, while Nilaparvata lugens （St^l） and its closely related species Nilaparvata muiri China were infected with two phylogeneticlly distant strains, wLug and wMui, respectively. Three new Wolbachia strains （provisionally named wMfasl, wMfas2 and wMfas3） were detected in the leafhopper Macrostelesfascifrons （Stal）. Only S. furcifera was co-infected with Cardinium, which indicated that the distribution of Cardinium in these rice planthop- pers was narrower than that of Wolbachia. Unambiguous intragenic recombination events among these Wolbachia strains and incongruent phylogenetic relationships show that the connections between different Wolbachia strains and hosts were more complex than we expected. These results suggest that horizontal transmission and host associated special- ization are two factors affecting Wolbachia and Cardinium infections among planthoppers and their related species.

关键词： Cardinium infection status intragenic recombination rice planthopper,Wolbachia

Fluorescence microscopic morphology and inhibition ratestudies on apoptosis of osteosarcoma cells induced by ^(153)Sm

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Nuclear Science and Techniques 2000年第4期11卷 263-266页

作者： ZHU Shou-Peng, xiao Dong, han xiao-Feng (Suzhou Medical College, Sizhou 215007) 苏州医学院江苏苏州 215007

The apoptosis of osteosarcoma cells treated with irradiation by 153Sm-EDTMP was studied. The morphological changes in osteosarcoma cells were observed by fluorescence microscopy. It was found that osteosarcoma cells exposed with 153Sm-EDTMP displayed significant nuclear fragmentation and marked pyknosis. With the prolongation of observing period, the membrane bound apoptotic bodies formation was observed. It should be noted, that with the lengthening of irradiation time by 153Sm-EDTMP, the inhibition rate of proliferation of osteosarcoma cells increased progressively.

关键词：钐153 ^153Sm-EDTMP 放射性核素标记荧光显微镜胃肉瘤肿瘤细胞细胞凋亡

Electron microscopic observations and DNA chain fragmentation studies on apoptosis in bone tumor cells induced by ^(153)Sm-EDTMP

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Nuclear Science and Techniques 1997年第3期8卷 163-165页

作者： Zhu Shou Peng, xiao Dong and han xiao Feng ( Suzhou Medical College, Suzhou 215007)

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关键词： DNA 胃癌细胞 153Sm-EDTMP

Profile of the RNA in exosomes from astrocytes and microglia using deep sequencing:implications for neurodegeneration mechanisms

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Neural Regeneration Research 2022年第3期17卷 608-617页

作者： Hui-Min Xie Xing Su Feng-Yuan Zhang Chao-Lun Dai Rong-Hua Wu Yan Li xiao-xiao han Xing-Mei Feng Bin Yu Shun-Xing Zhu Song-Lin Zhou Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education Co-Innovation Center of NeuroregenerationNantong UniversityNantongJiangsu ProvinceChina Department of Stomatology Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina Department of Neurosurgery Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina Medical College of Nantong University NantongJiangsu ProvinceChina Laboratory Animals Center Nantong UniversityNantongJiangsu ProvinceChina

Glial cells play an important role in signal transduction,energy metabolism,extracellular ion homeostasis and neuroprotection of the central nervous ***,few studies have explained the potential effects of exosomes from glial cells on central nervous system health and *** this study,the genes expressed in exosomes from astrocytes and microglia were identified by deep RNA *** Encyclopedia of Genes and Genomes analysis indicated that several pathways in these exosomes are responsible for promoting neurodegenerative diseases,including Alzheimer's disease,Parkinson's disease and Huntington's *** ontology analysis showed that extracellular exosome,mitochondrion and growth factor activity were enriched in exosomes from the unique astrocyte group,while extracellular exosome and mitochondrion were enriched in exosomes from the unique microglia ***,combined with the screening of hub genes,the protein-protein interaction network analysis showed that exosomes from astrocytes influence neurodegenerative diseases through metabolic balance and ubiquitin-dependent protein balance,whereas exosomes from microglia influence neurodegenerative diseases through immune inflammation and oxidative *** there were differences in RNA expression between exosomes from astrocytes and microglia,the groups were related by the hub genes,ubiquitin B and heat shock protein family A(Hsp70) member *** B appeared to be involved in pleiotropic regulatory functions,including immune regulation,inflammation inhibition,protein catabolism,intracellular protein transport,exosomes and oxidative *** results revealed the clinical significance of exosomes from glia in neurodegenerative *** study was approved by the Animal Ethics Committee of Nantong University,China(approval No.S20180102-152) on January 2,2018.

关键词： astrocyte bioinformatics analysis central nervous system exosomes microglia neurodegenerative disease RNA transcriptomics UBB

Extraction and Characterization of Humic Acids and Humin Fractions from a Black Soil of China

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Pedosphere 2005年第1期15卷 1-8页

作者： XING Bao-Shan, LIU Ju-Dong, LIU xiao-Bing and han xiao-ZengNortheast Institute of Geography and Agro-ecology, Chinese Academy of Sciences, Harbin 150040 (China). Department of Plant, Soil and Insect Sciences, University of Massachusetts, MA 01003 (USA) NE Inst. of Geogr. and Agro-Ecology Chinese Academy of Sciences Harbin 150040 China Dept. of Plant Soil and Insect Sci. University of Massachusetts MA 01003 United States

Twenty-three progressive extractions were performed to study individual humic acids (HAs) and humin fractions from a typical black soil (Mollisol) in Heilongjiang Province, China using elemental analysis and spectroscopic techniques. After 23 HA extractions the residue was separated into high and low organic carbon humin fractions. HA yield was the highest for the first extraction and then gradually decreased with further extractions. Organic carbon (OC) of the humin fractions accounted for 58% of total OC even after 23 successive HA extractions. In addition, the atomic C/H ratio decreased during the course of extraction while C/O increased; the E4/E6 ratio from the UV analysis decreased with further extraction while E2/E3 increased; the band assigned to aliphatic carbon (2930 cm-1) in the diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS) spectra gradually increased with progressive extraction; the calculated ratio of the sum of aromatic carbon peak heights to that of aliphatic carbon

关键词： elemental composition humic acid humin soil organic matter spectroscopic analysis

Pulmonary Multiple Nodules： Benign or Malignant？

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Chinese Medical Journal 2018年第16期131卷 1999-2001页

作者： Jing Liu xiao-Qiu Liu Bing-Di Yan Yan-Jun Xue xiao-xiao han han Li Li Ma Jie Zhang Jun-Ling Yang Department of Respiratory and Critical Care Medicine Second Hospital of Jilin University Changchun Jilin 130041 China

To the Editor： A case of primary thyroid fibrosarcoma with pulmonary metastasis is very rare. It is difficult to be diagnosed especially when pathological manifestation is atypical and early pulmonary metastasis coexists with some benign lesions. Here, we report that a case of thyroid fibrosarcoma with pulmonary and subcutaneous fat metastasis coexisting with solitary pulmonary sclerosing hemangioma （PSH） in lung. This report may improve better understanding of differential diagnosis of benign and malignant lesions primary thyroid fibrosarcoma in the lung.

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Impacts of land cover change on plant and bird species diversity in Hainan Island,China

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Journal of Environmental Sciences 1999年第2期11卷 100-103页

作者： Ouyang Zhi yun, Wang Ru song, Wang xiao ke, xiao han Research Center for Eco Environmental Sciences,Chinese Academy of Sciences,Beijing 100085, China

Hainan Island, located at the southern end of China, has less than 0.4% of land area but contains 13% of plant and animal species in China. During the last four decades many primary forests have been converted to shrub land, grassland, and tree planation (e.g., eucalyptus forest). As a result, area of primary tropical forest has been reduced from 25.8% in the 1950s to 4% in the 1990s. To assess impacts of land conversion on plant and bird species diversity, a series of samples in primary forest and four types of converted lands were took. The land conversion had tremendously reduced both plant and bird species diversity. Specifically, plant species richness per site was 83.7 in primary forest, 28.3 in shrub land, 12.5 in grassland, 14.4 in eucalyptus forest, and 21.4 in Acacia forest. Bird species richness showed a similar trend: 22.0 in primary forest, 14.5 in shrub land, 2.5 in grassland, 4.9 in eucalyptus forest, and 9.0 in Acacia forest. The Shannon species diversity indices for plants in the five types of land cover were 3.69, 1.99, 0.97, 1.47 and 2.07, respectively. Similarly, the Shannon indices for bird species diversity were the highest in primary forest, and lowest in grassland, and intermediate in shrub land and eucalyptus forest.

关键词： land cover change species diversity Hainan Island.

Study on localization of implanted electrode in brain tissue

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Study on localization of implanted electrode in brain tissue

Revolutionizing photothermal CO_(2)hydrogenation with ceria-based catalysts

中国神经科学学会第十二届全国学术会议

作者： Yong PENG Yang LIU Pei-hua SU xiao-xiao han Ting-ting WANG Li-jiao WANG Department of Biomedical Engineering Yanshan University

Objective In brain science research, the method of implantable electrode stimulation has been widely used in experiments to reveal the relationship between nervous system activity and specific behavior or cognitive function. In order to investigate the localization of implanted electrodes in brain tissues, this study was conducted with carp as the research object. Methods Craniotomy was carried out. The stainless-steel electrodes were implanted in carp cerebellums by stereotaxic instrument. The locus of brain electrode implantation was labeled with blue dots using the pre-fixation method of brain samples combined with Prussian blue color reaction. The paraffin sections were prepared, and then the brain electrode implantation sites were observed in histologically. Results（1） The brain tissue specimens is intact and the structure is neat.（2） The labeled points of stainless electrodes were clearly displayed by Prussian blue reaction. Conclusion The stainless-steel electrodes can be labeled by the color labeling method of Prussian blue in carp brain tissue sections.

关键词： Brain stereotaxic localization implantable brain electrodes anterior fixation of brain specimens blue dot marker

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Nano Research 2025年第1期18卷 30-49页

作者： Zhaorui Zhang xiao han Junhong Zhang Yunyun Dong Jinsheng Zhao Qian Xu ingqiang Zhang School of Chemistry and Chemical Engineering Liaocheng UniversityLiaocheng 252059China Institute for Catalysis Hokkaido UniversityN-21W-10Sapporo 001-0021Japan National Synchrotron Radiation Laboratory University of Science and Technology of ChinaHefei 230029China

In the context of achieving carbon neutrality,CO_(2)catalytic conversion technologies are effective in reducing atmospheric CO_(2)concentrations while simultaneously producing renewable *** approach is seen as a viable method for constructing a new carbon cycle,thereby effectively addressing the issue of global *** CO_(2)conversion has recently emerged as a promising research focus due to its high energy utilization efficiency,superior CO_(2)conversion,excellent product selectivity,mild operating conditions,low energy consumption and minimal operating *** oxide(CeO_(2))has been widely used in photothermal catalysis due to its unique chemical properties,particularly when used as a support material with supported metals,creating distinctive interfacial sites for catalytic *** an emerging star support in photothermal CO_(2)conversion,its contributions are equally significant and should not be ***,to our knowledge,there has been no comprehensive review of CeO_(2)applications in catalytic hydrogenation of CO_(2).In this paper,we summarize and discuss CeO_(2)-based materials for photothermal CO_(2)conversion to value-added *** research particularly focuses on the synthesis methods of CeO_(2)-supported catalysts,the history of CeO_(2)in photothermal catalysis,types of photothermal catalytic reactors,unique characterization methods for the photothermal catalysts and the photothermal CO_(2)hydrogenation reactions by CeO_(2)-based *** related to further challenges and future directions for photothermal CO_(2)hydrogenation are also provided.

关键词： CO_(2)neutrality hydrogenation photothermal catalysis ceria C1 chemistry