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GreenPhos,a universal method for in-depth measurement of plant phosphoproteomes with high quantitative reproducibility

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Molecular Plant 2024年第1期17卷 199-213页

作者： xiaoxiao Duan Yuanya Zhang Xiahe huang xiao Ma hui Gao Yan Wang Zhen xiao Chengcheng huang Zhongshu Wang Bolong Li Wenqiang Yang Yingchun Wang State Key Laboratory of Molecular Developmental Biology Institute of Genetics and Developmental BiologyChinese Academy of SciencesBejing 100101China University of Chinese Academy of Sciences Bejing 100049China Key Laboratory of Photobiology Instute of BotanyChinese Academy of SciencesBejing 100093China

Protein phosphorylation regulates a variety of important cellular and physiological processes in ***-depth profiling of plant phosphoproteomes has been more technically challenging than that of animal *** is largely due to the need to improve protein extraction efficiency from plant cells,which have a dense cell wall,and to minimize sample loss resulting from the stringent sample clean-up steps required for the removal of a large amount of biomolecules interfering with phosphopeptide purification and mass spectrometry *** this end,we developed a method with a streamlined workflow for highly efficient purification of phosphopeptides from tissues of various green organisms including Arabidopsis,rice,tomato,and Chlamydomonas reinhardtii,enabling in-depth identification with high quantitative reproducibility of about 11000 phosphosites,the greatest depth achieved so far with single liquid chromatography-mass spectrometry(LC-MS)runs operated in a data-dependent acquisition(DDA)*** mainstay features of the method are the minimal sample loss achieved through elimination of sample clean-up before protease digestion and of desalting before phosphopeptide enrichment and hence the dramatic increases of time-and *** method,named GreenPhos,combined with single-shot LC-MS,enabled in-depth quantitative identification of Arabidopsis phosphoproteins,including differentially phosphorylated spliceosomal proteins,at multiple time points during salt stress and a number of kinase substrate *** is expected to serve as a universal method for purification of plant phosphopeptides,which,if samples are further fractionated and analyzed by multiple LC-MS runs,could enable measurement of plant phosphoproteomes with an unprecedented depth using a given mass spectrometry technology.

关键词： plant phosphoproteomics phosphopeptides LC–MS Arabidopsis salt stress

Preparation of Seeding Type Immobilized Microorganisms and Their Degradation Characteristics on Di-n-Butyl Phthalate

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Biomedical and Environmental Sciences 2006年第2期19卷 147-152页

作者： LIN WANG QI-FANG LUO JIN-hui ZHAO xiao-HE ZHANG LI-JING huang Institute of Environmental Medicine School of Public Health Tongji Medical College HuaTj~ong University of Science ＆ Technology Wuhan 430030 Hubei China

Objective To study the preparation of seeding type immobilized microorganisms and their degradation characteristics on di-n-butyl phthalate （DBP）. Methods Diatomite, clinoptilolite, silk zeolite, and coal fly ash were chosen as reserved materials and modified. Their adsorption capacity and intensity in the bacteria were determined and the best carrier was picked out. The seeding type immobilized microorganisms were prepared by the best carrier and then it degraded DBP under different primary concentration, vibration rate, pH, temperature in the presence of metal compounds. Results The adsorption capacity of the modified coal fly ash, silk zeolite, clinoptilolite and zeolite was 44.2%, 71.6%, 84.0%, and 94.4%, respectively, which was 1.66, 1.49, 1.37, and 1.16 times as high as that of their natural state. Their adsorption intensity was 72.1%, 90.5%, 90.1%, and 91.1% in turn. The modified diatomite was selected to prepare the seeding type immobilized microorganisms. When the primary DBP concentration was 100 to 500 mg/L, the DBP-degraded rate of the immobilized microorganisms could be above 80%. The degradation activity of both the dissociative and immobilized microorganisms was higher in vibration than in stillness. When pH was 6.0 to 9.0, the DBP-degraded rate of the immobilized microorganisms was above 82%, which was higher than the dissociative microorganisms. When the temperature was between 20~C and 40~C, the DBP-degraded rate could reach 84.5% in 24 h. The metal compounds could inhibit the degradation activity of both the dissociative and immobilized microorganisms. The degradation process of the immobilized microorganisms could be described by the first-order model. Conclusion The adsorption capacity of the diatomite, clinoptilolite, silk zeolite and coal fly ash on DBP-degrading bacteria can be improved obviously after they are modified. The modified diatomite is best in terms of its adsorption capacity and intensity. Its seeding type immobilized microorganis

关键词： Carrier Modify Seeding type immobilized microorganism Biodegradation DBP

Permineralized Calamitean Axes from the Upper Permian of Xinjiang, Northwest China and Its Paleoecological Implication

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Journal of Earth Science 2018年第2期29卷 237-244页

作者： Fayao Chen xiao Shi Jianxin Yu Hongfei Chi Jun Zhu hui Li Cheng huang State Key Laboratory of Biogeology and Environmental Geology School of Earth Sciences China University of Geosciences College of Earth Sciences Jilin University Key-Lab for Evolution of Past Life and Environment in Northeast Asia Ministry of Education Jilin University

Two anatomically preserved calamitean axes are reported for the first time from the Late Permian Wutonggou Formation in the southern Bogda Mountains, Xinjiang Uygur Autonomous Re- gion, Northwest China. Based on the anatomical features, these axes are assigned to Arthropitys. A new species Arthropitys taoshuyuanensis sp. nov. is established. A. taoshuyuanensis sp. nov. possesses a large pith and comprises large pith cavity and a narrow perimedullary zone at the nodes and diaphragms at the internodes. Carinal canals are circular and surrounded by a single layer of metaxylem tracheids. Secondary xylem is divided into interfascicular rays and fascicular wedges. Interfascicular rays are ini- tially four to five cells wide and taper abruptly centrifugally. Fascicular wedge consists of thick-walled tracheids and thin-walled fascicular ray cells. Radial tracheid walls have uniseriate or biseriate circular pits, or scalariform pits. The absence of growth rings in the Arthropitys specimens indicates that they probably lived in the wetland area under stable annual temperature and water sufficient conditions.

关键词： Arthropitys calamitean horsetail Late Permian Xinjiang.

Chiral Recognition and Photoreaction of β-Amino Acids with Iridium(III)Complexes

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Chinese Journal of Chemistry 2022年第24期40卷 2909-2918页

作者： Ming-Feng Xiong Hai-Yun Zhou xiao-Kang huang Jing-Yan Fan Bao-hui Ye MOE Key Laboratory of Bioinorganic and Synthetic Chemistry School of ChemistrySun Yat-sen UniversityGuangzhouGuangdong510275 China Instrumental Analysis and Research Center Sun Yat-sen UniversityGuangzhouGuangdong510275 China

β-Amino acids(AAs),homologs ofα-AAs,are important building blocks of biological ***,chiral recognitions ofβ-AAs with Ir(III)complexes are reported,in favor of formation of the thermodynamically stableΛ-[Ir(pq)_(2)(D-β-AAs)]andΔ-[Ir(pq)_(2)(L-β-AAs)](pq is 2-phenylquinoline)*** photoreactions of[Ir(pq)_(2)(β-AA)]complexes are observed in an EtOH solution in the presence of O_(2) at room *** primaryβ-AAs complexes,such as rac-[Ir(pq)_(2)(β-ala)](β-ala isβ-alanine),Δ-[Ir(pq)_(2)(D-β-ma)]andΛ-[Ir(pq)_(2)(D-β-ma)](β-ma isβ-methylalanine),Δ-[Ir(pq)_(2)(D-β-pa)]andΛ-[Ir(pq)_(2)(D-β-pa)](β-pa isβ-phenylalanine),and rac-[Ir(pq)_(2)(β-dma)](β-dma is 3,3-dimethyl-β-alanine),are interligand C—N cross-coupling in situ between pq andβ-AAs *** secondaryβ-AA complexesΔ-[Ir(pq)_(2)(L-β-pro)]andΛ-[Ir(pq)_(2)(L-β-pro)](β-pro isβ-proline,2-(pyrrolidin-2-yl)acetic acid)are dehydrogenatively oxidized into imino acid complexesΔ-[Ir(pq)_(2)(L-β-pro-2H^(β)’)]andΛ-[Ir(pq)_(2)(L-β-pro-2H^(β)’)](L-β-pro-2H^(β)’=2-(3,4-dihydro-2H-pyrrol-2-yl)acetic acid),***,the dehydrogenative reaction inΔ-[Ir(pq)_(2)(L-β-pro)]diastereomer is regioselective depending on the reaction temperature,affordingΔ-[Ir(pq)_(2)(L-β-pro-2H^(β)’)]andΔ-[Ir(pq)_(2)(L-β-pro-2H^(β))](β-pro-2H^(β)=2-(3,4-dihydro-2H-pyrrol-5-yl)acetic acid)at low *** chiral recognitions and photoreactions of Ir(III)-β-AAs complexes are much different from the previous observations in Ir(III)-α-AAs complexes.

关键词： Photooxidation Chiral recognition Regioselectivity Enantioselectivity β-Amino acid

Positron emission tomography imaging for the assessment of mild traumatic brain injury and chronic traumatic encephalopathy:recent advances in radiotracers

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Neural Regeneration Research 2022年第1期17卷 74-81页

作者： Chu-Xin huang Yan-hui Li Wei Lu Si-Hong huang Meng-Jun Li Li-Zhi xiao Jun Liu Department of Radiology the Second Xiangya Hospital of Central South UniversityChangshaHunan ProvinceChina Department of Neurology the Second Xiangya Hospital of Central South UniversityChangshaHunan ProvinceChina PET-CT Center the Second Xiangya Hospital of Central South UniversityChangshaHunan ProvinceChina

A chronic phase following repetitive mild traumatic brain injury can present as chronic traumatic encephalopathy in some cases,which requires a neuropathological examination to make a definitive *** emission tomography(PET)is a molecular imaging modality that has high sensitivity for detecting even very small molecular changes,and can be used to quantitatively measure a range of molecular biological processes in the brain using different radioactive *** changes have also been reported in patients with different forms of traumatic brain injury,especially mild traumatic brain injury and subsequent chronic traumatic ***,PET provides a novel approach for the further evaluation of mild traumatic brain injury at molecular *** this review,we discuss the recent advances in PET imaging with different radiotracers,including radioligands for PET imaging of glucose metabolism,tau,amyloid-beta,γ-aminobutyric acid type A receptors,and neuroinflammation,in the identification of altered neurological *** novel radiolabeled ligands are likely to have widespread clinical application,and may be helpful for the treatment of mild traumatic brain ***,PET functional imaging with different ligands can be used in the future to perform largescale and sequential studies exploring the time-dependent changes that occur in mild traumatic brain injury.

关键词： amyloid-β chronic traumatic encephalopathy flumazenil fluorodeoxyglucose mild traumatic brain injury positron emission tomography tau protein traumatic brain injury

Multi-terminal pectin/chitosan hybrid electrolyte gated oxide neuromorphic transistor with multi-mode cognitive activities

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Frontiers of physics 2024年第5期19卷 117-127页

作者： Yan Li You Jie huang Xin Li Chen Wei Sheng Wang Xin huang hui xiao Li Qiang Zhu School of Physical Science and Technology Ningbo UniversityNingbo 315211China Ningbo Institute of Materials Technology and Engineering Chinese Academy of SciencesNingbo 315201China

In order to fulfill the urgent requirements of functional products,circuit integration of different functional devices are commonly ***,issues including production cycle,cost,and circuit crosstalk will get *** computing aims to break through the bottle neck of von Neumann *** devices with multi-operation modes,especially neuromorphic devices with multi-mode cognitive activities,would provide interesting ***,pectin/chitosan hybrid electrolyte gated oxide neuromorphic transistor was *** extremely strong proton related interfacial electric-double-layer coupling,the device can operate at low voltage of below 1 *** device can also operate at multi-operation mode,including bottom gate mode,coplanar gate and pseudo-diode ***,the artificial synapse can work at low voltage of only 1 mV,exhibiting extremely low energy consumption of~7.8 fJ,good signal-to-noise ratio of~229.6 and sensitivity of~23.6 *** inhibitory and excitatory synaptic responses were mimicked on the pseudo-diode,demonstrating spike rate dependent plasticity ***,a linear classifier is proposed on the oxide neuromorphic transistor under synaptic metaplasticity *** results suggest great potentials of the oxide neuromorphic devices with multi-mode cognitive activities in neuromorphic platform.

关键词： pectin/chitosan hybrid electrolyte pseudo-diode function multi-mode cognitive activities ultrasensitive oxide neuromorphic device linear data classifier

Insider Trading with a Random Deadline under Partial Observations:Maximal Principle Method

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Acta Mathematicae Applicatae Sinica 2022年第4期38卷 753-762页

作者： Kai xiao Yong-hui ZHOU School of Mathematical Sciences Guizhou Normal UniversityGuiyang 550001China School of Big Data and Computer Science Guizhou Normal UniversityGuiyang 550001China

For a revised model of Caldentey and Stacchetti(Econometrica,2010)in continuous-time insider trading with a random deadline which allows market makers to observe some information on a risky asset,a closed form of its market equilibrium consisting of optimal insider trading intensity and market liquidity is obtained by maximum principle *** shows that in the equilibrium,(i)as time goes by,the optimal insider trading intensity is exponentially increasing even up to infinity while both the market liquidity and the residual information are exponentially decreasing even down to zero;(ii)the more accurate information observed by market makers,the stronger optimal insider trading intensity is such that the total expect profit of the insider is decreasing even go to zero while both the market liquidity and the residual information are decreasing;(iii)the longer the mean of random time,the weaker the optimal insider trading intensity is while the more both the residual information and the expected profit are,but there is a threshold of trading time,half of the mean of the random time,such that if and only if after it the market liquidity is increasing with the mean of random time increasing.

关键词： continuous-time insider trading random deadline partial observations filtering theory maximal principle

Changes in vegetation and soil properties during recovery of a subtropical forest in South China

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Journal of Mountain Science 2018年第1期15卷 46-58页

作者： huang Fang-fang ZHANG Wei-qiang GAN Xian-hua huang Yu-hui GUO Yue-dong WEN xiao-ying Guangdong Provincial Key Laboratory of Silviculture Protection and Utilization Guangdong Academy of Forestry

Secondary forests account for a large amount of subtropical forest due to persistent anthropogenic disturbance in *** interaction between vegetation and soil during recovery process is rather complex and dependent on forest *** how vegetation and soil properties changes and how their relationship develops in secondary forests is key to effective forest restoration and *** we explored the patterns of vegetation and soil properties as well as their correlations during forest recovery process in a subtropical forest in south *** of three forest types,i.e.,broadleaf-conifermixedforest,broadleaved forest and old growth stand,were established to represent the recovery *** results showed that diversity patterns in the tree,shrub and herb layers were different:in the tree layer the species diversity peaked at the intermediate stage,while in the understory layers it decreased *** of the soil factors showed an increasing trend,and different effects of soil factors were found for the three layers as well as for the two spatial ***,our results suggested that vegetation and soil might be interdependent during the recovery *** studies are needed on exploring how vegetation interplays with soil at different scales and how nutrient limitations affects the vegetation development in a chronosequence.

关键词： Forest recovery Vegetation change Soil property Subtropical Succession

Low-temperature synthesis of SiC nanowires with Ni catalyst

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Rare Metals 2019年第3期38卷 206-209页

作者： Wei-Li Xie xiao-Dong Zhang Wen-hui Liu Qi Xie Guang-Wu Wen xiao-xiao huang Jian-Dong Zhu Fei-Xiang Ma School of Material Science and Engineering Harbin Institute of Technology Department of Prosthodontics School of StomatologyHarbin Medical University School of Material Science and Engineering Harbin Institute of Technology at Weihai

SiC nano wires were fabricated on the silicon substrate dipped with a layer of Ni catalyst at 900 ℃ by gas pressure annealing processing. The morphologies and crystal structures were determined by scanning electron microscopy(SEM), transmission electron microscopy(TEM)and X-ray diffraction(XRD). The results show that the assynthesized nanowires are β-SiC single crystalline with diameter range of 50-100 nm, and length of tens of micron by directly annealing at 900 ℃. The SiC nano wires grow along the [111] direction with highly uniform morphology. And the possible growth mechanism of SiC nano wires is *** present work provides an efficient strategy for the production of high-quality SiC nano wires.

关键词： SiC nanowires Single crystalline silicon Ni catalyst Growth mechanism