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New 3-(4-arylpiperazin-1-yl)-1-(benzo[b]thiophen-3-yl)-2-methylpropanol derivatives:Synthesis and evaluation for dual 5-HT1A/SSRI activities

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Chinese Chemical Letters 2008年第4期19卷 412-414页

作者： Ai Jun Li xiao hua zhang Xue Qin Zhou Dong Zhi Liu School of Chemical Engineering and Technology Tianjin UniversityTianjin 300072China College of Chemical and Pharmaceutical Engineering Hebei University of Science and TechnologyShijiazhuang 050018China

A series of 3-（4-arylpiperazin-1-yl）-1-（benzo[b]thiophen-3-yl）-2-methylpropanol derivatives were designed and synthesized based on 5-HT1A/SSRI drugs design *** synthesized compounds were evaluated for their dual 5... 详细信息

关键词： Antidepressants Arylpiperazines 5-HT1A/SSRI 5-HT transporter 5-HT1A receptor

Cultivation of uncultured marine microorganisms

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Marine Life Science & Technology 2021年第2期3卷 117-120页

作者： Fengping Wang Meng Li Li huang xiao-hua zhang State Key Laboratory of Microbial Metabolism School of OceanographySchool of Life Sciences and BiotechnologyShanghai Jiao Tong UniversityShanghai200240China Shenzhen Key Laboratory of Marine Microbiome Engineering Institute for Advanced StudyShenzhen UniversityShenzhen518060China Key Laboratory of Microbial Resources Institute of Microbiology Chinese Academy of SciencesBeijing100101China College of Marine Life Sciences and Institute of Evolution and Marine Biodiversity Ocean University of ChinaQingdao266003China

Microorganisms(both pro-and eukaryotes)occur in all marine environments with an estimated abundance of 10^(3)–10^(10)cells/cm^(3)in sediments and 10^(4)–10^(7)cells/ml in seawater;in these habitats they play key roles in marine biogeochemical cycles(Overmann and Lepleux 2016).Surveys employing molecular marker-based high-throughput sequencing and sophisticated metagenomic approaches have revealed the vast diversity of microorganisms in marine environments and have substantially expanded the tree of life(Castelle and Banfield 2018;Hug et al.2016;Nayfach et al.2020).However,many of the novel lineages have no cultured *** fact,most(>99%)of the marine microorganisms have not been cultured under laboratory conditions(Hofer 2018;Jiao et al.2020)and are seen as“microbial dark matter”(Lok 2015).Although culture-independent approaches have enabled elucidation of the marine microbial assemblies and their metabolic potentials,pure cultures remain needed for microbiological *** example,pure cultures are critical for an understanding of the morphology,ecophysiology,and biochemistry of these organisms,which are key to recognizing their functions in complex environmental ***,cultures are needed for discovering new bioactive substances,such as anti-tumor bioactive substances,new antibiotics and other secondary metabolites(Ding et al.2020).

关键词： marine environments expanded

Effect of siRNA on Wisp-1 gene expression,proliferation,migration and adhesion of mouse hepatocellular carcinoma cells

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Asian Pacific Journal of Tropical Medicine 2015年第10期8卷 803-809页

作者： Jian Ge xiao-hua zhang Fang Wang Yu Wang Qing-Yan Li Wei Tao Guo-hua Ren Department of Gastroenterology Affiliated Shandong Provincial Hospital of Shandong University School of Nursing Binzhou Vocational College Department of Gastroenterology Zhangqiu People's Hospital Department of Gastroenterology General Hospital of Ningxia Medical University Department of Medical Oncology Shandong Cancer Hospital and Institute

Objective:To study the inhibition effect of siRNA on the expression of Wisp-1 gene in Hca-F of mouse hepatocellular carcinoma cells strain and also its effect on the proliferation,migration and adhesion of hepatocellular carcinoma ***:Three expression vectors of siRNA were ***2000 was employed to transfect Hca-F cells and Western blot was used to detect the inhibition effect of siRNA on the expression of Wisp-1 ***,CCK8 was adopted to detect the effect of Wisp-1 siRNA on the proliferation of Hca-F cells;Annexin V-FTTC/PI double staining flow cytometry was used to detect the effect of Wisp-1 siRNA on the apoptosis of Hca-F cells;Transwell was used to detect the effect of Wisp-1 siRNA on the migration of Hca-F *** in vitro cell adhesion kit was used to detect of Wisp-1 siRNA on the change in the components of extracellular matrix to which Hca-F cells *** blot was used to detect the activation of protein kinase B(AKT)/glycogen synthase kinase-3(3pathway and the expression of downstream target protein p53 and matrix metal!***:The siRNA showed interference effect on the expression of Wisp-1 *** with the control group,after being transfected to cells,Wisp-1 siRNA could significantly inhibit the proliferation,migration and adhesion of Hca-F cells and also promote the cell apoptosis,which was related to the down-regulated phosphorylation of AKT and glycogen synthase kinase-3β and the expression of p53 and matrix metalIoproteinases-2(P<0.05).Conclusions:The inhibition of Wisp-1 expression can reduce the proliferation,migration and adhesion of mouse hepatocellular carcinoma cells,which is related to the AKT/ glycogen synthase kinase-3 β ***-1 gene may be the potential target to cure the hepatocellular carcinoma.

关键词： Hepatocellular carcinoma cells Wisp-1 RNA interfer

Adipsin inhibits Irak2 mitochondrial translocation and improves fatty acid β-oxidation to alleviate diabetic cardiomyopathy

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Military Medical Research 2024年第5期11卷 625-642页

作者： Meng-Yuan Jiang Wan-Rong Man Xue-Bin zhang xiao-hua zhang Yu Duan Jie Lin Yan zhang Yang Cao De-Xi Wu xiao-Fei Shu Lei Xin Hao Wang xiao zhang Cong-Ye Li xiao-Ming Gu Xuan zhang Dong-Dong Sun Department of Cardiology Xijing HospitalAir Force Medical UniversityXi’an710032China Department of Basic Medicine Air Force Medical UniversityXi’an710032China Department of Physiology and Pathophysiology Air Force Medical UniversityXi’an710032China Institute for Hospital Management Research Chinese PLA General HospitalBeijing100853China

Background Diabetic cardiomyopathy (DCM) causes the myocardium to rely on fatty acid β-oxidation for energy. The accumulation of intracellular lipids and fatty acids in the myocardium usually results in lipotoxicity, which impairs myocardial function. Adipsin may play an important protective role in the pathogenesis of DCM. The aim of this study is to investigate the regulatory effect of Adipsin on DCM lipotoxicity and its molecular *** high-fat diet (HFD)-induced type 2 diabetes mellitus model was constructed in mice with adipose tissue-specific overexpression of Adipsin (Adipsin-Tg). Liquid chromatography-tandem mass spectrometry (LC–MS/MS), glutathione-S-transferase (GST) pull-down technique, Co-immunoprecipitation (Co-IP) and immunofluorescence colocalization analyses were used to investigate the molecules which can directly interact with Adipsin. The immunocolloidal gold method was also used to detect the interaction between Adipsin and its downstream *** expression of Adipsin was significantly downregulated in the HFD-induced DCM model (P < 0.05). Adipose tissue-specific overexpression of Adipsin significantly improved cardiac function and alleviated cardiac remodeling in DCM (P < 0.05). Adipsin overexpression also alleviated mitochondrial oxidative phosphorylation function in diabetic stress (P < 0.05). LC–MS/MS analysis, GST pull-down technique and Co-IP studies revealed that interleukin-1 receptor-associated kinase-like 2 (Irak2) was a downstream regulator of Adipsin. Immunofluorescence analysis also revealed that Adipsin was co-localized with Irak2 in cardiomyocytes. Immunocolloidal gold electron microscopy and Western blotting analysis indicated that Adipsin inhibited the mitochondrial translocation of Irak2 in DCM, thus dampening the interaction between Irak2 and prohibitin (Phb)-optic atrophy protein 1 (Opa1) on mitochondria and improving the structural integrity and function of mitochondria (P < 0.05). Interestingly, in

关键词： Diabetic cardiomyopathy Mitochondrial translocation Mitochondrial function Fatty acidβ-oxidation

Simulation and Experimentation for Low Density Drain AlGaN/GaN HEMT

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Chinese Physics Letters 2014年第3期31卷 177-180页

作者： WANG Chong HE Yun-Long DING Ning ZHENG Xue-Feng zhang Peng MA xiao-hua zhang Jin-Cheng HAO Yue Key Lab of Wide Band Gap Semiconductor Materials and Devices and Institute of Microelectronics Xidian University Xi'an 710071

In order to improve the breakdown voltage of AlGaN/GaN high electron mobility transistors （HEMTs）, we report a feasible method of low density drain （LDD） HEMT. The fluoride-based plasma treatment using CF4 gas is performed on the drain-side of the gate edge. The channel two-dimensional electron gas （2DEG） concentrations are modulated by fluoride plasma treatment, and the peak electric field at the gate edge is effectively reduced, so the breakdown voltage is improved. The electric field distributions of the LDD-HEMTs are simulated using the Silvaco software, and the peak of the electric field on the gate edge is effectively reduced. Experimental results show that, compared with the conventional HEMT, LDD-HEMTs have a lower reverse leakage current of the gate, and the breakdown voltage is increased by 36%. The current collapse characteristics of the LDD-HEMTs are confirmed by dual-pulse measurement, and an obvious pulse current reduction is due to the surface states by implanting F ions between the gate and the drain.

关键词： HIGH electron mobility transistors RESEARCH GALLIUM nitride PLASMA (Ionized gases) -- Research CHEMICAL vapor deposition PHYSICS research

Influence of Trace Amount of Calponin on Smooth Muscle Myosin in Different States

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Acta Biochimica et Biophysica Sinica 2004年第8期36卷 529-536页

作者： Jing-Xian YANG, xiao-hua FENG, Ying zhang, and Yuan LIN Department of Pharmacology, Dalian Medical University, Dalian 116027, China Department of Pharmacology Dalian Medical UniversityDalian 116027China

Calponin (CaP), a thin filament-associated protein, is thought to be involved in modulating smooth muscle contractile activity, but the role and mechanism keep unknown. In this study, trace amount of calponin (TAC) was found to obviously influence myosin in different states in Ca2+-independent manner, suggesting a high efficient interaction between TAC and myosin. In this assay, the lowest ratio of CaP vs. myosin was 1:10,000, with the concentration of CaP 10,000-fold lower than that used previously. Myosin phosphorylation, myosin Mg2+-ATPase activity and protein binding activity were detected to determine the effects of TAC on the myosin in different states. The amount of precipitated myosin that bound to TAC was used as the index to determine the interaction between myosin and TAC in binding assay. Our data showed that in the absence of actin, TAC significantly increased the precipitation of unphosphorylated myosin, Ca2+- dependently or independently phosphorylated myosin by MLCK, and stimulated the Mg2+-ATPase activities of these myosins slightly but significantly. However, no obvious change of precipitation of myosin phospho- rylated by PKA was observed, indicating the relatively selective effect of TAC. In the presence of actin, the increase of myosin precipitations was abolished, and no obvious change of actin precipitations and actin- activated myosin Mg2+-ATPase activities were observed implicating the high efficiency of TAC on myosin being present in the absence of actin. Although we can not give conclusive comments to our results, we propose that the high efficiency of TAC-myosin interaction is present when actin is dissociated from myosin, even if CaP/myosin ratio is very low; this high efficient interaction can be abolished by actin. However, why and how TAC can possess such a high efficiency to influence myosin and how the physiological significance of the high efficiency of TAC is in regulating the interaction between myosin and actin remain to be inve

关键词： trace amount of calponin protein binding phosphorylation myosin Mg2+-ATPase

Effect of multiple parameters on the supersonic gas-jet target characteristics for laser wakefield acceleration

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Nuclear Science and Techniques 2021年第7期32卷 90-98页

作者： Qiu-Shi Liu Ming-Jiang Ma Bao-Zhen Zhao xiao-hua zhang Chong Lv Xiang-Hao Meng Ji zhang xiao-Na Ban Zhao Wang xiao-Feng Xi Bao-Xian Tian Chuang-Ye He Bing Guo Department of Nuclear Physics China Institute of Atomic EnergyBeijing 102413China

The supersonic gas-jet target is an important experimental target for laser wakefield acceleration(LWFA),which has great potential for driving novel radiation sources such as betatron radiation and Compton scattering gamma *** to different electron acceleration requirements,it is necessary to provide specific supersonic gas jets with different density profiles to generate electron beams with high quality and high repetition *** this study,the interference images and density profiles of different gas-jet targets were obtained through a modified Nomarski interference diagnosis *** relationships between the gas density and back pressure,nozzle structure,and other key parameters were *** with different characteristics are conducive to meeting the various requirements of LWFA.

关键词： Supersonic gas-jet target Laser wakefield acceleration Electron acceleration Optical interference diagnosis

microRNA-455-5p alleviates neuroinflammation in cerebral ischemia/reperfusion injury

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Neural Regeneration Research 2022年第8期17卷 1769-1775页

作者： Jian-Song zhang Pin-Pin Hou Shuai Shao Anatol Manaenko Zhi-Peng xiao Yan Chen Bing Zhao Feng Jia xiao-hua zhang Qi-Yong Mei Qin Hu Central Laboratory Renji HospitalShanghai Jiao Tong University School of MedicineShanghaiChina Department of Neurosurgery Renji HospitalShanghai Jiao Tong University School of MedicineShanghaiChina Department of Neurology The First Affiliated Hospital of Chongqing Medical UniversityChongqingChina Department of Medical Genetics Shanghai Jiao Tong University School of MedicineShanghaiChina Department of Neurosurgery Changzheng Hospitalthe Second Military Medical UniversityShanghaiChina

Neuroinflammation is a major pathophysiological factor that results in the development of brain injury after cerebral ischemia/*** of microRNA(miR)-455-5p after ischemic stroke has been considered a potential biomarker and therapeutic target for neuronal injury after ***,the role of miR-455-5p in the post-ischemia/reperfusion inflammatory response and the underlying mechanism have not been *** this study,mouse models of cerebral ischemia/reperfusion injury were established by transient occlusion of the middle cerebral artery for 1 hour followed by ***-455-5p,antagomir-455-5p,and their negative controls were injected intracerebroventricularly 2 hours before or 0 and 1 hour after middle cerebral artery occlusion(MCAO).The results showed that cerebral ischemia/reperfusion decreased miR-455-5p expression in the brain tissue and the peripheral ***-455-5p pretreatment increased miR-455-5p expression in the brain tissue,reduced the cerebral infarct volume,and improved neurological ***,primary cultured microglia were exposed to oxygen-glucose deprivation for 3 hours followed by 21 hours of reoxygenation to mimic cerebral ischemia/***-455-5p reduced C-C chemokine receptor type 5 mRNA and protein levels,inhibited microglia activation,and reduced the production of the inflammatory factors tumor necrosis factor-αand interleukin-1β.These results suggest that miR-455-5p is a potential biomarker and therapeutic target for the treatment of cerebral ischemia/reperfusion injury and that it alleviates cerebral ischemia/reperfusion injury by inhibiting C-C chemokine receptor type 5 expression and reducing the neuroinflammatory response.

关键词： agomiR-455-5p biomarker blood-brain barrier C-C chemokine receptor type 5 ischemia/reperfusion injury ischemic stroke microglia microRNA-455-5p neuroinflammation pretreatment

Induction of Protection against Paraquat-induced Oxidative Damage by Abscisic Acid in Maize Leaves is Mediated through Mitogen-activated Protein Kinase

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Journal of Integrative Plant Biology 2009年第10期51卷 961-972页

作者： Hai-Dong Ding xiao-hua zhang Shu-Cheng XU Li-Li Sun Ming-Yi Jiang A-Ying zhang Yin-Gen Jin College of Life Sciences Nanjing Agricultural University Nanjing 210095 China College of Biosciences and Biotechnology Yangzhou University Yangzhou 225009 China College of Life Sciences Fuyang Teachers College Fuyang 236032 China National Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural University Nanjing 210095 China

Mitogen-activated protein kinase （MAPK） cascade has been shown to be important components in stress signal transduction pathway. In the present study, protection of maize seedlings （Zea mays L.） against paraquat-generated oxidative toxicity by abscisic acid （ABA）, its association with MAPK and ZmMPK5, a candidate for MAPK were investigated. Treatment of maize leaves with exogenous ABA led to significant decreases in the content of malondialdehyde, the percentage of ion leakage and the level of protein oxidation （in terms of carbonyl groups） under paraquat （PQ） stress. However, such decreases were blocked by the pretreatment with two MAPK kinase inhibitors PD98059 and U0126. The damage caused by PQ was further aggravated by inhibitors. Two inhibitors also suppressed the total activities of the antioxidant enzymes superoxide dismutase （SOD, EC 1.***.1.1）, catalase （CAT, EC 1.***.1.6）, ascorbate peroxidase （APX, EC ***）, and glutathione reductase （GR, EC 1.***.4.2）. Besides, treatment with PQ stimulated the activation of a 46 kDa MAPK, which was identified as ZmMPK5 by in-gel kinase assay with immunoprecipitation. These results reveal that ABA-induced protection against PQ-generated oxidative damage is mediated through MAPK cascade in maize leaves, in which ZmMPK5, a candidate for MAPK, is demonstrated to be involved.

关键词： abscisic acid mitogen-activated protein kinase oxidative damage paraquat Zea mays