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Acupuncture promotes functional recovery after cerebral hemorrhage by upregulating neurotrophic factor expression

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Neural Regeneration Research 2020年第8期15卷 1510-1517页

作者： Dan Li Qiu-Xin Chen Wei Zou xiao-Wei Sun Xue-Ping Yu xiao-hong dai Wei Teng Third Department of Acupuncture First Affiliated HospitalHeilongjiang University of Chinese MedicineHarbinHeilongjiang ProvinceChina Department of Acupuncture Third Affiliated HospitalBeijing University of Chinese MedicineBeijingChina

Acupuncture is widely used in the treatment of cerebral hemorrhage,and it improves outcomes in experimental animal models and ***,the mechanisms underlying the effectiveness of acupuncture treatment for cerebral hemorrhage are still *** this study,a model of intracerebral hemorrhage was produced by injecting 50μL autologous blood into the caudate nucleus in Wistar *** at Baihui(DU20)and Qubin(GB7)acupoints was performed at a depth of 1.0 inch,12 hours after blood injection,once every 24 *** needle was rotated at 200 r/min for 5 minutes,For each 30-minute session,needling at 200 r/min was performed for three sessions,each lasting 5 *** the positive control group,at 6 hours,and 1,2,3 and 7 days after induction of hemorrhage,the rats were intraperitoneally injected with 1 mL aniracetam(0.75 mg/mL),three times a *** Bederson behavioral test was used to assess palsy in the contralateral *** blot assay was used to examine the expression levels of Nestin and basic fibroblast growth factor in the basal *** was performed to count the number of Nestin-and glial cell line-derived neurotrophic factor-positive cells in the basal *** effectively reduced hemorrhage and brain edema,elevated the expression levels of Nestin and basic fibroblast growth factor in the basal ganglia,and increased the number of Nestin-and glial cell line-derived neurotrophic factor-positive cells in the basal ***,these findings suggest that acupuncture promotes functional recovery after cerebral hemorrhage by increasing the expression of neurotrophic *** study was approved by the Committee for Experimental Animals of Heilongjiang Medical Laboratory Animal Center(approval No.2017061001)on June 10,2017.

关键词： acupuncture basic fibroblast growth factor brain cell protection cerebral hemorrhage electron microscope glial cell line-derived neurotrophic factor immunohistochemistry Nestin western blot assay

Property(ω_1) and Single Valued Extension Property

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Journal of Mathematical Research and Exposition 2010年第6期30卷 1009-1014页

作者： Chen Hui SUN xiao hong CAO Lei dai College of Mathematics and Information Science Shaanxi Normal UniversityShaanxi 710062P.R.China

In this note we study the property（ω1）,a variant of Weyl＇s theorem by means of the single valued extension property,and establish for a bounded linear operator defined on a Banach space the necessary and sufficient condition for which property（ω1） *** a consequence of the main result,the stability of property（ω1） is discussed.

关键词： property(ω1) single valued extension property.

Baihui(DU20)-penetrating-Qubin(GB7) acupuncture inhibits apoptosis in the perihemorrhagic penumbra

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Neural Regeneration Research 2018年第9期13卷 1602-1608页

作者： Beng Zhang xiao-hong dai Xue-Ping Yu Wei Zou Wei Teng xiao-Wei Sun Wei-Wei Yu Hao Liu Hui Wang Meng-Juan Sun Meng Li Heilongjiang University of Chinese Medicine Harbin Heilongjiang Province China First Affiliated Hospital Heilongjiang University of Chinese Medicine Harbin Heilongjiang Province China Clinical Key Laboratory of Integrated Traditional Chinese and Western Medicine Heilongjiang University of Chinese Medicine HarbinHeilongjiang Province China Department of Acupuncture and Moxibustion Tongde Hospital of Zhejiang Province Hangzhou Zhejiang Province China

Baihui（DU20）-penetrating-Qubin（GB7） acupuncture can inhibit inflammatory reactions and activate signaling pathways related to proliferation after intracerebral ***,there is no research showing the relationship between this treatment and cell *** models of intracerebral hemorrhage were established by injecting 60 μL of autologous blood into the right side of the *** hours later,the needle traveled subcutaneously from the Baihui acupoint to Qubin *** needle was alternately rotated（180 ± 10 turns/min） manually along clockwise and counter-clockwise *** lasted for 7 days,and was performed three times each for 6 minutes with 6-minute intervals between *** intraperitoneally receiving Sonic hedgehog pathway activator,purmorphamine（1 mg/kg per day）,served as positive *** and sensory function were assessed using the Ludmila Belayev *** of pathological changes were measured in the perihemorrhagic penumbra using hematoxylin-eosin *** was examined by terminal deoxynucleotidyl transferase（Td T）-mediated d UTP nick end labeling *** of smoothened（Smo） and glioma-associated homolog 1（Gli1） was determined by western blot *** results showed that Baihui-penetrating-Qubin acupuncture promoted recovery of motor and sensory function,reduced the apoptotic cell percentage in the perihemorrhagic penumbra,and up-regulated Smo and Gli1 *** conclude that Baihui-penetrating-Qubin acupuncture can mitigate hemorrhage and promote functional recovery of the brain in a rat model of intracerebral hemorrhage,possibly by activating the Sonic hedgehog pathway.

关键词： nerve regeneration acupuncture treatment intracerebral hemorrhage neurological function Ludmila Belayev test TUNEL assay purmorphamine Smo Ptchl Glil neural regeneration

钨对铸态Ti-45Al-8Cr-2Nb合金的高温性能影响

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铸造 2007年第10期56卷 1082-1085页

作者：肖代红中南大学粉末冶金国家重点实验室湖南长沙410083

采用高温拉伸测试及显微组织观察,研究了微量钨对铸态Ti-45Al-8Cr-2Nb(原子百分比)合金的高温性能影响。结果表明,在基体合金中添加2%W,能使铸态合金的最大伸长率降低,而最大伸长率相应的温度向高温方向移动;高温变形过程中,晶粒滑动的... 详细信息

采用高温拉伸测试及显微组织观察,研究了微量钨对铸态Ti-45Al-8Cr-2Nb(原子百分比)合金的高温性能影响。结果表明,在基体合金中添加2%W,能使铸态合金的最大伸长率降低,而最大伸长率相应的温度向高温方向移动;高温变形过程中,晶粒滑动的协调过程由晶界扩散转化为体扩散;含钨的合金在800℃时的伸长率从0.62%提高到90%。

关键词： Ti-45Al-8Cr-2Nb合金钨高温性能显微组织

Acupuncture through Baihui (DU20) to Qubin (GB7) mitigates neurological impairment after intracerebral hemorrhage

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Neural Regeneration Research 2018年第8期13卷 1425-1432页

作者： xiao-Ying Liu xiao-hong dai Wei Zou Xue-Ping Yu Wei Teng Ying Wang Wei-Wei Yu Hui-Hui Ma Qiu-Xin Chen Peng Liu Rui-Qiao Guan Shan-Shan Dong Heilongjiang University of Chinese Medicine Harbin Heilongjiang Province China The First Affiliated Hospital of Heilongjiang University of Chinese Medicine Harbin Heilongjiang Province China Clinical Key Laboratory of Integrated Traditional Chinese and Western Medicine of Heilongjiang University of Chinese Medicine HarbinHeilongjiang Province China Department of Pharmacology of Dali University Dali Yunnan Province China

Inflammation plays an important role in nerve defects caused by intracerebral hemorrhage. Repairing brain damage by inhibiting the macrophage-inducible C-type lectin/spleen tyrosine kinase （Mincle/Syk） signaling pathway is a potential new target for treating cerebral hemorrhage. In this study, we aimed to determine whether acupuncture through Baihui （DU20） to Qubin （GBT） is an effective treatment for intracerebral hemorrhage through the Mincle/Syk signaling pathway. An intracerebral hemorrhage rat model was established by autol- ogous blood infusion into the caudate nucleus. Acupuncture through Baihui to Qubin was performed for 30 minutes, once every 12 hours, for a total of three times. Piceatannol （34.62 mg/kg）, a Syk inhibitor, was intraperitoneally injected as a control. Modified neurological severity score was used to assess neurological function. Brain water content was measured. Immunohistochemistry and western blot assay were used to detect immunoreactivity and protein expression levels of Minde, Syk, and CARD9. Real-time polymerase chain reaction was used to determine interleukin-1[~ mRNA levels. Hematoxylin-eosin staining was performed to observe histopathological changes. Our re- suits showed that acupuncture through Baihui to Qubin remarkably improved neurological function and brain water content, and inhibited immunoreactivity and expression of Mincle, Syk, CARDg, and interkeukin-1β Moreover, this effect was similar to piceatannol. These find- ings suggest that acupuncture through Baihui to Qubin can improve neurological impairment after cerebral hemorrhage by inhibiting the Mincle/Syk signaling pathway.

关键词： nerve regeneration intracerebral hemorrhage acupuncture Baihui (DU20) Qubin (GBT) inflammation Mincle/Syk signaling pathway nerve protection neural regeneration

添加W对Ti-Al-Cr-Nb铸态合金高温变形的影响

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中南大学学报（自然科学版） 2007年第6期38卷 1039-1044页

作者：肖代红宋旼中南大学粉末冶金国家重点实验室湖南长沙410083

通过非自耗电弧熔炼及氩气保护浇铸方法,合成不同W含量的Ti-Al-Cr-Nb合金。采用高温拉伸测试及显微组织观察,研究添加W对Ti-Al-Cr-Nb铸态合金的显微组织及其高温变形影响。研究结果表明:W的添加使Ti-Al-Cr-Nb合金的铸态组织得到细化;在... 详细信息

通过非自耗电弧熔炼及氩气保护浇铸方法,合成不同W含量的Ti-Al-Cr-Nb合金。采用高温拉伸测试及显微组织观察,研究添加W对Ti-Al-Cr-Nb铸态合金的显微组织及其高温变形影响。研究结果表明:W的添加使Ti-Al-Cr-Nb合金的铸态组织得到细化;在800℃时基体合金的伸长率从0.62%提高到90%;在高温变形过程中,添加W使Ti-Al-Cr-Nb铸态合金的最大伸长率从620%降低到200%,而最大伸长率相应的温度从850℃增大到1 050℃。不同W含量的Ti-Al-Cr-Nb铸态合金在800～1100℃的高温变形机制主要是晶粒滑动,而添加W使晶粒滑动的协调过程由晶界扩散转化为体扩散。

关键词： Ti-Al-Cr-Nb合金 W 高温变形显微组织

Cu和Mg含量对Al-Cu-Mg-Ag合金组织与性能的影响

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金属热处理 2007年第12期32卷 64-66页

作者：宋旼肖代红中南大学粉末冶金国家重点实验室湖南长沙410083

通过力学试验及透射电镜研究了不同Cu和Mg含量对Al-Cu-Mg-Ag合金室温、高温力学性能以及显微组织的影响。结果表明,提高Cu和Mg的含量可以提高合金在室温及高温条件下的屈服强度和抗拉强度,但伸长率下降。透射电子显微分析表明,增加Cu和M... 详细信息

通过力学试验及透射电镜研究了不同Cu和Mg含量对Al-Cu-Mg-Ag合金室温、高温力学性能以及显微组织的影响。结果表明,提高Cu和Mg的含量可以提高合金在室温及高温条件下的屈服强度和抗拉强度,但伸长率下降。透射电子显微分析表明,增加Cu和Mg的含量将提高时效过程中强化相的形核密度与体积分数,从而提高合金的强度。

关键词： Al-Cu-Mg-Ag合金力学性能显微组织

铸造Ti-47Al-8Cr-2Nb合金的低温超塑性及其组织演变

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中国有色金属学报 2008年第10期18卷 1749-1755页

作者：肖代红黄伯云中南大学粉末冶金国家重点试验室长沙410083

通过非自耗电弧熔炼及氩气保护浇铸,制备高Cr含量的Ti-47Al-8Cr-2Nb合金。采用金相观察、扫描电镜、透射电镜及高温拉伸测试等实验方法,研究铸态合金在800～1000℃的超塑性变形能力及其变形机制。结果表明,高Cr合金化的铸造Ti-47Al-8Cr-... 详细信息

通过非自耗电弧熔炼及氩气保护浇铸,制备高Cr含量的Ti-47Al-8Cr-2Nb合金。采用金相观察、扫描电镜、透射电镜及高温拉伸测试等实验方法,研究铸态合金在800～1000℃的超塑性变形能力及其变形机制。结果表明,高Cr合金化的铸造Ti-47Al-8Cr-2Nb合金显示出低温超塑性。在850℃的低温及应变速率1×10^-4/s时的最大伸长率为630%,应变速率敏感系数m达到0.51。在800～1000℃的变形激活能为245kJ/mol。合金的低温超塑性变形机制是由晶界扩散控制和β→γ相转变而协调的晶界滑动,β相在变形过程中起到了重要的作用。

关键词：钛铝合金铸造超塑性显微组织

SiC_p/AlCuMgAgCe复合材料的显微组织与力学性能

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铸造 2007年第12期56卷 1277-1280页

作者：肖代红黄伯云中南大学粉末冶金国家重点实验室湖南长沙410083

采用压铸浸渗法制备了53%SiCp增强的AlCuMgAgCe基复合材料。通过X射线衍射、金相观察、扫描电镜与透射电镜分析以及力学性能测试,研究了SiCp/AlCuMgAgCe复合材料的显微组织与力学性能。结果显示,在AlCuMgAgCe合金中加入53%体积分数的Si... 详细信息

采用压铸浸渗法制备了53%SiCp增强的AlCuMgAgCe基复合材料。通过X射线衍射、金相观察、扫描电镜与透射电镜分析以及力学性能测试,研究了SiCp/AlCuMgAgCe复合材料的显微组织与力学性能。结果显示,在AlCuMgAgCe合金中加入53%体积分数的SiC颗粒后,复合材料的组织致密,分布均匀,其断裂方式包括界面脱开、基体韧断和增强体开裂,SiC颗粒与基体之间并没有发生明显的界面反应。在185℃的等温时效析出过程中,复合材料的析出相主要由Ω相和少量θ′相组成。

关键词：铝基复合材料 SiC颗粒显微组织时效力学性能