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Activation of autophagy by Citri Reticulatae Semen extract ameliorates amyloid-beta-induced cell death and cognition deficits in Alzheimer’s disease

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Neural Regeneration Research 2024年第11期19卷 2467-2479页

作者： Yong Tang Jing Wei xiao-fang wang Tao Long xiaohong Xiang Liqun Qu Xingxia wang Chonglin Yu Xingli xiao Xueyuan Hu Jing Zeng Qin Xu Anguo Wu Jianming Wu Dalian Qin xiaogang Zhou Betty Yuen-Kwan Law Dr.Neher’s Biophysics Laboratory for Innovative Drug Discovery State Key Laboratory of Quality Research in Chinese MedicineMacao University of Science and TechnologyMacao Special Administrative RegionChina Sichuan Key Medical Laboratory of New Drug Discovery and Druggability Evaluation Luzhou Key Laboratory of Activity Screening and Druggability Evaluation for Chinese Materia Medica Southwest Medical UniversityLuzhouSichuan ProvinceChina Eye School and Key Laboratory of Sichuan Province Ophthalmopathy Prevention&Cure and Visual Function Protection of Chengdu University of TCM ChengduSichuan ProvinceChina School of Basic Medical Sciences Southwest Medical UniversityLuzhouSichuan ProvinceChina Department of Ophthalmology The Affiliated Hospital of Southwest Medical UniversityLuzhouSichuan ProvinceChina Department of Neurology The First People’s Hospital of NeijiangNeijiangSichuan ProvinceChina

Amyloid-beta-induced neuronal cell death contributes to cognitive decline in Alzheimer’s *** Reticulatae Semen has diverse beneficial effects on neurodegenerative diseases,including Parkinson’s and Huntington’s diseases,however,the effect of Citri Reticulatae Semen on Alzheimer’s disease remains *** the current study,the anti-apoptotic and autophagic roles of Citri Reticulatae Semen extract on amyloid-beta-induced apoptosis in PC12 cells were first *** Reticulatae Semen extract protected PC12 cells from amyloid-beta-induced apoptosis by attenuating the Bax/Bcl-2 ratio via activation of *** addition,Citri Reticulatae Semen extract was confirmed to bind amyloid-beta as revealed by biolayer interferometry in vitro,and suppress amyloid-beta-induced pathology such as paralysis,in a transgenic Caenorhabditis elegans in vivo ***,genetically defective Caenorhabditis elegans further confirmed that the neuroprotective effect of Citri Reticulatae Semen extract was *** importantly,Citri Reticulatae Semen extract was confirmed to improve cognitive impairment,neuronal injury and amyloid-beta burden in 3×Tg Alzheimer’s disease *** revealed by both in vitro and in vivo models,these results suggest that Citri Reticulatae Semen extract is a potential natural therapeutic agent for Alzheimer’s disease via its neuroprotective autophagic effects.

关键词： Alzheimer’s disease amyloid-beta apoptosis autophagy Caenorhabditis elegans Citri Reticulatae Semen

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Recombinant Human IgG antibodies against Human Cytomegalovirus

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Biomedical and Environmental Sciences 2008年第5期21卷 372-380页

作者： TAO DUAN xiao-fang wang SHU-YUAN xiao SHU-YAN GU AND MI-fang LIANG Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 100052 China Department of Pathology and Center for Biodefence and Emerging Infectious Diseases University of Texas Medical Branch Galveston Texas 77555-0609. USA

Objective To study the passive immunization with human monoclonal antibodies as for prophylaxis of human cytomegalovirus （HCMV） infection. Methods Fab monoclonal antibodies to HCMV were recovered by repertoire cloning of mRNA from a HCMV infected individual. Antigen binding specificity, CDR sequence of VH and VL and neutralizing activity on HCMV AD169 stain were analyzed in vitro. The light and heavy chain Fd fragment genes of Fab antibodies were further cloned into a recombinant baculovirus expression vector pAC-K-Fc to express intact IgG. Secreted products were purified with affinity chromatography using protein G. Results SDS-PAGE and Western blot confirmed the expression of the intact IgG. Immuno-blotting and -precipitation were used to identify HCMV proteins. One Fab monoclonal antibody recognized a conformational HCMV protein. Conclusion IgG antibodies can neutralize the HCMV AD169 strain efficiently at a titer of 2.5 μg/mL and may prove valuable for passive immunoprophylaxis against HCMV infection in humans.

关键词： Human cytomegalovirus Human engineering antibody Phage display Recombinant baculovirus expression

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Thermo-reversible MWCNTs/Epoxy Polymer for Use in Self-healing and Recyclable Epoxy Adhesive

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Chinese Journal of Polymer Science 2017年第6期35卷 728-738页

作者： Ya-kun Guo Han Li Peng-xiang Zhao xiao-fang wang Didier Astruc 帅茂兵 Institute of Materials China Academy of Engineering Physics Department of Materials Southwest University of Science and Technology Institut des Sciences Moleculaires University Bordeaux

A self-healing and recyclable carbon tube/epoxy adhesive was prepared by epoxy monomer with Diels-Alder （DA） bonds, diethylenetriamine and polyethyleneimine modified multi-wall carbon nanotubes （MWCNTs）. The self-healing and recyclable ability was attained by thermally reversible Diels-Alder reaction between furan and maleimide in the epoxy monomer. By controlling the molar ratio of furfuryl glycidyl ether and 4,4＇-methylenebis（N-phenylmaleimide）, the glass transition temperature and mechanical properties of MWCNTs/epoxy adhesives were varied. The self-healing properties of MWCNTs/epoxy polymers were evaluated by lap shear experiment and the results showed that the MWCNTs/epoxy adhesives exhibited enhanced mechanical properties and excellent self-healing ability under heat stimulus. The healing efficiency was related to the molecule mobility and the conversion of DA reaction between furan and maleimide. The MWCNTs/epoxy adhesives also displayed excellent recyclable ability by transforming into soluble polymer under heating. These materials offer a wide range of possibilities to produce materials with healing and recyclable ability and have the potential to bring great benefits to our daily lives by enhancing the safety, performance, and lifetime of products.

关键词： Self-healing Recyclable Epoxy adhesives MWCNTs Diels-Alder reaction

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Plastic limit load analysis for pressure pipe with incomplete welding defects based on the extended Net Section Collapse Criteria

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2010年第6期11卷 440-448页

作者： Zhi-jiang JIN Cheng-hang JIANG Xian-ping WAN Po CHEN xiao-fang wang Institute of Chemical Process Equipment Zhejiang University Hangzhou 310027 China

With von Mises yield criterion,the loading range of Net Section Collapse(NSC) Criteria is extended from combined tension and bending loadings to combined bending,torsion and internal pressure loadings.A new theoretical analyzing method of plastic limit load for pressure pipe with incomplete welding defects based on the extended NSC Criteria is presented and the correlative formulas are deduced,the influences of pipe curvature,circumferential length and depth of incomplete welding defects on the plastic limit load of pressure pipe are considered as well in this ***,according to the orthogonal experimental design method,the plastic limit loads are calculated by the finite element method and compared with the theoretical *** results show that the expressions of plastic limit load of pressure pipe with incomplete welding defects under bending,torsion and internal pressure based on extended NSC criteria are *** study provides an important theoretical basis for the establishment of safety assessment measure towards pressure pipe with incomplete welding defects.

关键词： Net Section Collapse(NSC) Criteria Incomplete welding defects Pressure pipe Plastic limit load Finite element

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A new active piggyBac-like element in Aphis gossypii

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Insect Science 2011年第6期18卷 652-662页

作者： Guang-Hua Luo Min Wu xiao-fang wang Wei Zhang Zhao-Jun Han Key Laboratory of Integrated Management of Crop Diseases and Pest Ministry of Education College of Plant Protection Department of Biochemistry and Molecular Biology College of Life Science Nanjing Agricultural University Nanjing China

Nine piggyBac-like elements （PLEs） were identified from the cotton aphid Aphis gossypii Glover. All the PLEs shared high sequence similarity with each other. However, eight of the nine PLEs were unlikely to encode functional transposase due to the existence of disruptive mutations within the coding regions. The other one PLE contained major characteristics of members in the piggyBac family, including TTAA target site duplications, inverted terminal repeats （ITRs）, and an open reading frame （ORF） coding for a transposase with a putative DDD domain. This one with an intact transposase ORF was named AgoPLE1.1. The predicted transposase shared 47% similarity with that of Trichoplusia ni piggyBac IFP2. Phylogenetic analyses showed that AgoPLE1.1 was most related to the Heliothis virescens PLE1.1 （HvPLE1.1） element, with 45% and 60% similarity at the nucleotide and amino acid levels, respectively. A functional assay demonstrated that AgoPLE1.1 encoded a functional transposase and was able to cause precise excision in cell cultures. On the other hand, few genomic insertion polymorphisms of AgoPLEI were observed in the genome of the cotton aphid. These observations suggested that AgoPLE1.1 was a PLE that invaded the cotton aphid genome in recent periods and retained its activity.

关键词： Aphis gossypii piggyBac-like element (PLE) transposon

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Application of the Modified nverse Design Method in the Optimization of the Runner Blade of a Mixed-Flow Pump

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Chinese Journal of Mechanical Engineering 2018年第6期31卷 137-153页

作者： Ye-Ming Lu xiao-fang wang Wei wang fang-Ming Zhou School of Energy and Power Engineering Dalian University of TechnologyDalian 116000China Key Laboratory of Ocean Energy Utilization and Energy Conservation of Ministry of Education Dalian University of TechnologyDalian 116000China Collaborative Innovation Center of Major Machine Manufacturing in Liaoning Province Dalian 116000China

To improve the design speed and reduce the design cost for the previous blade design method, a modified inverse design method is presented. In the new method, after a series of physical and mathematical simplifications, a sail?like constrained area is proposed, which can be used to configure di erent runner blade shapes. Then, the new method is applied to redesign and optimize the runner blade of the scale core component of the 1400?MW canned nuclear coolant pump in an established multi?optimization system compromising the Computational Fluid Dynamics(CFD) analysis, the Response Surface Methodology(RSM) and the Non?dominated Sorting Genetic Algorithm?II(NSGA?II). After the execution of the optimization procedure, three optimal samples were ultimately obtained. Then, through comparative analysis using the target runner blade, it was found that the maximum e ciency improvement reached 1.6%, while the head improvement was about 10%. Overall, a promising runner blade inverse design method which will benefit the hydraulic design of the mixed?flow pump has been proposed.

关键词： Optimization Mixed?flow pump Inverse design method Runner blade Nuclear coolant

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The importance of a potential phosphorylation site in enamelin on enamel formation

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International Journal of Oral Science 2017年第4期9卷 210-215页

作者： Wen-Juan Yan Pan Ma Ye Tian Jing-Ya wang Chun-Lin Qin Jian Q Feng xiao-fang wang Department of Biomedical Sciences and Center for Craniofacial Research and Diagnosis Texas A&M University College of DentistryDallasUSA Department of Endodontics Nanfang HospitalSouthern Medical UniversityGuangzhouChina

Enamelin （ENAM） has three putative phosphoserines （pSers） phosphorylated by a Golgi-associated secretory pathway kinase （FAM20C） based on their distinctive Ser-x-Glu （S-x-E） motifs. Fam2OC-knockout mice show severe enamel defects similar to those in the Enam-knockout mice, implying an important role of the pSers in ENAM. To determine the role of pSer5s in ENAM, we characterized ENAMRgsc514 mice, in which Sers5 cannot be phosphorylated by FAM20C due to an E57〉Gs7 mutation in the S-x-E motif, The enamel microstructure of 4-week-old mice was examined by scanning electron microscopy. The teeth of 6-day-old mice were characterized by histology and immunohistochemistry. The protein lysates of the first lower molars of 4-day-old mice were analyzed by Western immunoblotting using antibodies against ENAM, ameloblastin and amelogenin. ENAMRgsc514 heterozygotes showed a disorganized enamel microstructure, while the homozygotes had no enamel on the dentin surface. The N-terminal fragments of ENAM in the heterozygotes were detained in the ameloblasts and localized in the mineralization front of enamel matrix, while those in the WT mice were secreted out of ameloblasts and distributed evenly in the outer 1/2 of enamel matrix. Surprisingly, the 15 kDa C-terminal fragments of ameloblastin were not detected in the molar lysates of the homozygotes. These results suggest that the phosphorylation of SerSS may be an essential posttranslational modification of ENAM and is required for the interaction with other enamel matrix molecules such as ameloblastin in mediating the structural organization of enamel matrix and protein-mineral interactions during enamel formation.

关键词： ameloblastin enamel enamelin FAM20C phosphorylation phosphoserine

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Buckling Properties of Water-Drop-Shaped Pressure Hulls with Various Shape Indices Under Hydrostatic External Pressure

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China Ocean Engineering 2024年第1期38卷 1-17页

作者： DING xiao-de ZHANG Jian wang fang JIAO Hui-feng wang Ming-lu School of Mechanical Engineering Jiangsu University of Science and TechnologyZhenjiang 212003China Shanghai Engineering Research Center of Marine Renewable Energy College of Engineering Science and Technol-ogyShanghai Ocean UniversityShanghai 201306China China Ship Scientific Research Center Wuxi 214082China

The water-drop-shaped pressure hull has a good streamline,which has good application prospect in the underwater ***,this study conducted analytical,experimental and numerical investigation of the buckling properties of water-drop-shaped pressure hulls under hydrostatic pressure.A water-drop experiment was conducted to design water-drop-shaped pressure hulls with various shape *** critical loads for the water-drop-shaped pressure hulls were resolved by using Mushtari’s *** numerical simulations including linear buckling analysis and nonlinear buckling analysis including eigenmode imperfections were *** results indicated that the critical loads resolved by Mushtari's formula were in good agreement with the linear buckling loads from the numerical *** formula can be extended to estimate the buckling capacity of water-drop-shaped pressure *** addition,three groups of pressure hulls were fabricated by using stereolithography,a rapid prototyping ***,three groups of the pressure hulls were subjected to ultrasonic measurements,optical scanning,hydrostatic testing and numerical *** experimental results were consistent with the numerical *** results indicate that the sharp end of the water-drop-shaped pressure hulls exhibited instability compared with the blunt *** paper provides a new solution to the limitations of experimental studies on the water-drop-shaped pressure hulls as well as a new configuration and evaluation method for underwater observatories.

关键词： buckling pressure hull water drop underwater observatory

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Laser manufacturing of spatial resolution approaching quantum limit

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Light(Science & Applications) 2024年第3期13卷 421-428页

作者： xiao-Jie wang Hong-Hua fang Zhen-Ze Li Dan wang Hong-Bo Sun State Key Laboratory of Precision Measurement Technology and Instruments Department of Precision InstrumentTsinghua UniversityBeijing 100084China State Key Laboratory of Integrated Optoelectronics College of Electronic Science and EngineeringJilin University2699 Qianjin StreetChangchun 130012China

Atomic and close-to-atom scale manufacturing is a promising avenue toward single-photon emitters,single-electron transistors,single-atom memory,and quantum-bit devices for future communication,computation,and sensing *** manufacturing is outstanding to this end for ease of beam manipulation,batch production,and no requirement for *** is,however,suffering from optical diffraction ***,we report a spatial resolution improved to the quantum limit by exploiting a threshold tracing and lock-in method,whereby the two-order gap between atomic point defect complexes and optical diffraction limit is surpassed,and a feature size of<5 nm is *** underlying physics is that the uncertainty of local atom thermal motion dominates electron excitation,rather than the power density slope of the incident *** show that the colour centre yield in hexagonal boron nitride is transformed from stochastic to deterministic,and the emission from individual sites becomes polychromatic to *** a result,single colour centres in the regular array are deterministically created with a unity yield and high positional accuracy,serving as a step forward for integrated quantum technological applications.

关键词： quantum limit resolution

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Effect of electrical parameters and slag system on macrostructure of electroslag ingot

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China Foundry 2024年第1期21卷 44-50页

作者： Bing-jie wang Yu wang Meng-jun wang Lei Zhao Li-zhong Chang xiao-fang Shi School of Metallurgy engineering Anhui University of TechnologyMa'anshan 243002AnhuiChina

To investigate the influence of electric parameters and slag system on the solidification quality of electroslag ingot during electroslag remelting,different power supply modes,current strengths and remelting slag systems were used to conduct electroslag remelting experiments on 304L austenitic stainless steel,and the macrostructure of electroslag ingots was *** results indicate that the depth of the metal pool decreases with the reduction of remelting frequency in the low frequency power supply *** effects of different power supply modes,such as low-frequency,direct current straight polarity(DCSP),and direct current reverse polarity(DCRP),on reducing the depth of the metal pool increase in that *** reducing the remelting current strength in the same power supply mode,the depth of metal pool is *** compared to the binary slag system of 70%CaF2+30%Al2O3,the ternary slag system of 60%CaF2+20%Al2O3+20%CaO is more effective in reducing the depth of the metal pool during *** the 60%CaF2+20%Al2O3+20%CaO ternary slag system results in a shallower and flatter metal pool,with columnar crystal growth occurring closer to the axial *** effect is observed for both low frequency and direct current(DC)power supply modes.

关键词： electroslag remelting frequency slag system solidification quality

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