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外源性骨髓间充质干细胞在糖尿病模型大鼠体内向胰腺迁移并分化为胰岛细胞的趋势

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中国组织工程研究与临床康复 2007年第15期11卷 2870-2873页

作者：肖红珍张慧芹刘阁玲河北医科大学唐山临床医学院内分泌科河北省唐山市063000

目的:观察尾静脉注射骨髓间充质干细胞在糖尿病模型大鼠体内定向迁移至胰腺组织的规律。方法:实验于2004-08/2006-03在唐山工人医院中心实验室,华北煤炭医学院动物中心完成。实验材料:青年SD大鼠购自华北煤炭医学院动物中心(动物级别:SP... 详细信息

目的:观察尾静脉注射骨髓间充质干细胞在糖尿病模型大鼠体内定向迁移至胰腺组织的规律。方法:实验于2004-08/2006-03在唐山工人医院中心实验室,华北煤炭医学院动物中心完成。实验材料:青年SD大鼠购自华北煤炭医学院动物中心(动物级别:SPF/VAF;许可证号:SCXK2002-0003)。实验方法:①采用密度梯度离心结合贴壁培养方法分离、纯化雄性大鼠骨髓间充质干细胞,并采用免疫组织荧光法鉴定。②将30只雌性大鼠完全随机分为3组:生理盐水组、骨髓间充质干细胞组、糖尿病模型组,每组10只。骨髓间充质干细胞组大鼠经尾静脉注射1×106L-1骨髓间充质干细胞0.2mL;生理盐水组大鼠尾静脉注射0.2mL生理盐水;糖尿病模型组检测大鼠各基础血糖值及体质量,按65mg/kg计算所需链脲佐菌素剂量,将其溶于柠檬酸钠缓冲液中,立即腹腔注射。用血糖仪及血糖试纸监测大鼠血糖,当血糖≥16.7mmol/L且稳定2d后经尾静脉注射1×106L-1骨髓间充质干细胞0.2mL。③应用原位杂交法检测雌性大鼠胰腺组织内Y染色体标志物及放射免疫法测定C-肽值。结果:30只大鼠均进入结果分析。①雌性大鼠腹腔注射链脲佐菌素后血糖检测:注射后2d血糖水平开始迅速升高,4d后血糖值大于16.7mmol/L且稳定在21mmol/L左右,30只大鼠均造模成功。②免疫荧光法检测骨髓间充质干细胞表型:呈抗Laminin染色阴性、抗CD44染色阳性。③放射免疫法检测雌性大鼠血清中C-肽值:与注射前比较,骨髓间充质干细胞组及糖尿病模型组注射骨髓间充质干细胞后,大鼠血清中C-肽值呈明显升高的趋势[注射前:(0.398±0.094),(0.263±0.011)μg/L;注射后:(0.443±0.109),(0.374±0.961)μg/L,P<0.05或P<0.01],生理盐水组无明显变化。④鼠胰腺组织石蜡切片中Y染色体标记物表达:生理盐水组大鼠胰腺组织中未见黄绿荧光Y染色体标记物存在,骨髓间充质干细胞组和糖尿病模型组大鼠胰腺组织中均可见黄绿荧光Y染色体标记物存在,糖尿病模型组大鼠胰腺组织中黄绿荧光强度较大、数量较多。结论:体外分离培养的骨髓间充质干细胞经尾静脉注射后,在糖尿病模型大鼠体内可迁移至胰腺组织中,并具有分化为胰岛素分泌细胞的可能性。

关键词：骨髓间充质干细胞迁移 Y染色体标志物糖尿病模型

肾包膜内注射骨髓间充质干细胞在链脲佐菌素糖尿病模型大鼠体内的定位及功能测评

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中国组织工程研究与临床康复 2007年第20期11卷 3924-3927页

作者：肖红珍李伟娟俞芳项岫秀张慧芹刘秀玲刘阁玲河北医科大学唐山临床学院内分泌一科河北省唐山市063000

目的:观察骨髓间充质干细胞在体外不诱导条件下,移植于链脲佐菌素所致糖尿病大鼠后在胰腺的定位及胰岛素分泌情况。方法:实验于2004-08/2006-03在2级实验室唐山工人医院中心实验室和具有国家认证的屏障动物环境实验室华北煤炭医学院动... 详细信息

目的:观察骨髓间充质干细胞在体外不诱导条件下,移植于链脲佐菌素所致糖尿病大鼠后在胰腺的定位及胰岛素分泌情况。方法:实验于2004-08/2006-03在2级实验室唐山工人医院中心实验室和具有国家认证的屏障动物环境实验室华北煤炭医学院动物中心完成。①实验材料:青年清洁级SD大鼠购自华北煤炭医学院动物中心(SCXK2002-0003),4周龄,体质量180～250g,动物级别:SPF/VAF。②实验方法:体外培养雄性SD大鼠骨髓间充质干细胞,并采用流式细胞仪鉴定。选取雌性SD大鼠,按65mg/kg剂量腹腔注射链脲佐菌素建立糖尿病大鼠模型。给药后二三天,大鼠血糖升高,当血糖≥16.7mmol/L且稳定2d,认为糖尿病模型建立成功。将建模成功的40只大鼠完全随机分为对照组、实验组,每组20只。对照组大鼠右侧肾包囊注射磷酸缓冲液0.2mL,实验组大鼠右侧肾包囊下注射骨髓间充质干细胞0.2mL,细胞数为4×105L-1。于移植前及移植后第3,5,7天采大鼠尾静脉血检测血糖。③实验评估:于移植前及注射后7d采大鼠心脏血检测C-肽值,移植后7d取大鼠胰腺组织作石蜡切片,应用原位杂交技术检测Y染色体细胞。结果:40只大鼠全进入结果分析。①骨髓间充质干细胞鉴定:骨髓间充质干细胞呈梭形、放射状,形成数个细胞克隆时排列整齐,原代培养7～10d即可长满,按1∶3传代,生长四五天达融合。流式细胞术表面标志鉴定显示,培养的原代骨髓间充质干细胞表面标志CD90阳性,基质细胞表面标志CD44阳性,造血细胞表面标志CD45及内皮细胞表面标志CD31阴性。②大鼠尾静脉血血糖值、心脏血C-肽值分析:血糖结果显示,对照组治疗后第3,5,7天血糖值及C-肽值与治疗前相比,无明显变化(P>0.05);实验组治疗后第3,5,7天血糖值较治疗前明显下降,治疗后第7天C-肽值明显上升,与对照组对应时间比较,差异均显著[实验组治疗前:(21.75±2.44)mmol/L,(0.21±0.01)μg/L;实验组治疗后:(12.23±1.95)mmol/L,(14.00±2.85)mmol/L,(12.75±2.86)mmol/L,(0.26±0.01)μg/L;对照组治疗后:(21.63±1.34)mmol/L,(23.03±1.11)mmol/L,(22.48±1.92)mmol/L,(0.21±0.01)μg/L,P均<0.01]。③原位杂交技术检测Y染色体细胞:对照组大鼠胰腺组织切片中未见黄绿荧光Y染色体,实验组大鼠胰腺组织切片中可见黄绿荧光Y染色体,存在于胰腺腺泡间隙,或胰腺组织中。结论:雄性大鼠骨髓间充质干细胞在肾包囊注射下可到达雌性糖尿病大鼠胰腺组织,使糖尿病大鼠血糖下降,C-肽值升高,具有分化为胰岛素分泌细胞的可能性。

关键词：骨髓间充质干细胞糖尿病 Y染色体移植

自体骨髓单个核细胞移植治疗下肢缺血性疾病中细胞因子的作用

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中国组织工程研究与临床康复 2007年第20期11卷 3928-3931页

作者：张文军肖红珍李伟娟张慧芹俞芳项岫秀刘阁玲河北医科大学唐山临床学院核医学科河北省唐山市063000 河北医科大学唐山临床学院内分泌一科河北省唐山市063000

目的:评估自体骨髓单个核细胞移植治疗下肢动脉缺血性疾病中几种细胞因子的作用。方法:实验于2006-09/12在河北医科大学唐山临床学院中心实验室完成。实验材料:日本大耳白兔40只,清洁级,5月龄,体质量2.5～3.0kg,由华北煤炭医学院动物中... 详细信息

目的:评估自体骨髓单个核细胞移植治疗下肢动脉缺血性疾病中几种细胞因子的作用。方法:实验于2006-09/12在河北医科大学唐山临床学院中心实验室完成。实验材料:日本大耳白兔40只,清洁级,5月龄,体质量2.5～3.0kg,由华北煤炭医学院动物中心提供(合格证为SCXK-2005-0002),采用完全随机设计方法将日本大耳白兔分为4组:对照组、缺血组、磷酸盐缓冲液组、骨髓单个核细胞治疗组,10只/组。实验方法:对照组不作任何干预措施,其他3组建立日本大耳白兔右下肢缺血模型。取右下肢缺血模型制备2周后的兔,分离获取骨髓单个核细胞。缺血组制备右下肢缺血模型后不作其他干预措施。磷酸盐缓冲液组模型制备2周后,用1mL注射器将0.01mol/L磷酸盐缓冲液分15点注射于右下肢股骨中点股内收肌群内。骨髓单个核细胞治疗组模型制备2周后,用1mL注射器将1×109L-1骨髓单个核细胞悬液分15点注射于右下肢股内收肌群内。实验评估:造模后3d切取内收肌组织标本,测量其组织匀浆中血管内皮生长因子、碱性成纤维细胞生长因子、白细胞介素1β的含量;4周后行数字减影血管造影术,术后取右下肢内收肌标本,采用CD34免疫组织化学法检测毛细血管密度,观察下肢血管再生情况。结果:40只日本大耳白兔均进入结果分析。①移植后4周腹主动脉造影检测:骨髓单个核细胞治疗组结扎股动脉处的远端毛细血管生成较明显,血管成网状。缺血组及磷酸盐缓冲液组结扎处远端毛细血管生成不明显。②CD34免疫组织化学方法检测毛细血管密度:对照组平均为16个/×400高倍镜,缺血组和磷酸盐缓冲液组平均为5个/×400高倍镜,治疗组毛平均为20.5个/×400高倍镜。骨髓单个核细胞治疗组肌肉标本毛细血管密度明显高于缺血组和磷酸盐缓冲液组(P0.05)。结论:自体骨髓单个核细胞移植治疗缺血性疾病过程中,白细胞介素1β对毛细血管的生成起主要作用。

关键词：骨髓干细胞自体移植下肢缺血

自体骨髓干细胞移植配合针灸治疗糖尿病周围神经病变患者24例双下肢神经电生理的变化

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中国组织工程研究与临床康复 2007年第11期11卷 2065-2067页

作者：张慧芹刘阁玲俞芳项岫秀李伟娟肖红珍刘秀玲河北医科大学唐山临床医学院内分泌科河北省唐山市063000

目的:观察2型糖尿病并发周围神经病变患者自体骨髓干细胞移植配合针灸治疗后,双下肢胫神经与腓总神经的电生理变化。方法:①选取河北医科大学唐山临床医学院2005-01/2006-12收治住院的确诊为糖尿病足或糖尿病下肢动脉闭塞症同时并发糖... 详细信息

目的:观察2型糖尿病并发周围神经病变患者自体骨髓干细胞移植配合针灸治疗后,双下肢胫神经与腓总神经的电生理变化。方法:①选取河北医科大学唐山临床医学院2005-01/2006-12收治住院的确诊为糖尿病足或糖尿病下肢动脉闭塞症同时并发糖尿病周围神经病变的24例患者,男16例,女8例,平均年龄(56.8±6.1)岁,平均病程(12.6±3.6)年,均无严重的心、肾、肝、肺病变,对本实验知情同意。随机数字表法分为干细胞组、针灸组、干细胞+针灸组,8例/组。②局麻下由双侧髂后上棘采集每位患者的骨髓血200～300mL,应用密度梯度离心分离骨髓血中单个核细胞,镜下计数单个核细胞总数约为1.1×1012L-1,用生理盐水稀释成30mL干细胞悬液。③干细胞组给予单纯自体骨髓干细胞移植治疗,在硬膜外麻醉下将干细胞混悬液沿闭塞血管两侧多点肌肉注射到患者下肢,进针深度约1.4cm,相邻两点间的距离及与闭塞血管间距离均为1.5cm,每点注射0.8～1.0mL,注射时避开血管与神经。针灸组给予单纯针灸治疗,用普通手法针刺足三里、环跳、阳陵泉、三阴交。针刺15min/次,1次/d,持续治疗30d。干细胞+针灸组行自体骨髓干细胞移植配合针灸治疗,过程同上。④使用肌电图仪检测治疗前及治疗后3个月各组患者双下肢胫神经与腓总神经的感觉神经传导速度、运动神经传导速度。结果:24例2型糖尿病并发周围神经病变患者均进入结果分析。①各组患者治疗后一般状况:干细胞+针灸组治疗24h,足趾疼痛感和麻木感明显减轻;1个月后疼痛消失,足趾可轻微活动;3个月后足趾活动力量可达3级,除2例患者有跛行外,其余6例患者均可正常行走。干细胞组、针灸组患者预后情况较干细胞+针灸组差。②各组治疗前后双下肢神经传导速度的变化:与治疗前比较,各组患者胫神经、腓总神经的运动神经传导速度均显著增加(P0.05)。组间比较,各组改善运动神经传导速度的效果差异无显著性意义(P>0.05);与干细胞组改善胫神经、腓总神经的感觉神经传导速度比较,针灸组治疗效果较差[(25.2±5.5),(20.1±4.9);(30.3±5.2),(20.3±5.4)m/s;P均<0.05],而干细胞+针灸组改善效果显著[(25.2±5.5),(29.4±8.5);(30.3±5.2),(31.7±7.2)m/s;P均<0.01]。结论:自体骨髓干细胞移植配合针灸治疗可显著改善糖尿病并发周围神经病变的电生理指标。

关键词：自体骨髓干细胞移植糖尿病周围神经病变针灸治疗神经电生理双下肢感觉神经传导速度运动神经传导速度下肢动脉闭塞症

Treadmill exercise in combination with acousto-optic and olfactory stimulation improves cognitive function in APP/PS1 mice through the brain-derived neurotrophic factor-and Cygb-associated signaling pathways

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Neural Regeneration Research 2025年第9期20卷 2706-2726页

作者： Biao xiao Chaoyang Chu Zhicheng Lin Tianyuan Fang Yuyu Zhou Chuxia Zhang Jianghui Shan Shiyu Chen Liping Li Department of Physiology and Pharmacology Health Science CenterNingbo UniversityNingboZhejiang ProvinceChina Ningbo Key Laboratory of Behavioral Neuroscience Health Science CenterNingbo UniversityNingboZhejiang ProvinceChina Key Laboratory of Addiction Research of Zhejiang Province NingboZhejiang ProvinceChina

A reduction in adult neurogenesis is associated with behavioral abnormalities in patients with Alzheimer's ***,enhancing adult neurogenesis represents a promising therapeutic approach for mitigating disease symptoms and ***,nonpharmacological interventions aimed at inducing adult neurogenesis are currently *** individual non-pharmacological interventions,such as aerobic exercise,acousto-optic stimulation,and olfactory stimulation,have shown limited capacity to improve neurogenesis and cognitive function in patients with Alzheimer's disease,the therapeutic effect of a strategy that combines these interventions has not been fully *** this study,we observed an age-dependent decrease in adult neurogenesis and a concurrent increase in amyloid-beta accumulation in the hippocampus of amyloid precursor protein/presenilin 1 mice aged 2-8 *** deposition became evident at 4 months,while neurogenesis declined by 6 months,further deteriorating as the disease ***,following a 4-week multifactor stimulation protocol,which encompassed treadmill running(46 min/d,10 m/min,6 days per week),40 hz acousto-optic stimulation(1 hour/day,6 days/week),and olfactory stimulation(1 hour/day,6 days/week),we found a significant increase in the number of newborn cells(5'-bromo-2'-deoxyuridine-positive cells),immature neurons(doublecortin-positive cells),newborn immature neurons(5'-bromo-2'-deoxyuridine-positive/doublecortin-positive cells),and newborn astrocytes(5'-bromo-2'-deoxyuridine-positive/glial fibrillary acidic protein-positive cells).Additionally,the amyloid-beta load in the hippocampus *** findings suggest that multifactor stimulation can enhance adult hippocampal neurogenesis and mitigate amyloid-beta neuropathology in amyloid precursor protein/presenilin 1 ***,cognitive abilities were improved,and depressive symptoms were alleviated in amyloid precursor protein/presenilin 1 mice

关键词： acousto-optic stimulation adult neurogenesis Alzheimer's disease amyloid precursor protein/presenilin 1 mice amyloid-beta deposition brain cell apoptosis cognitive impairment depression-like behavior involuntary treadmill exercise olfactory stimulation serum metabolites

Cloning and identification of an angiostatic molecule IP-10/crg-2

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World Journal of Gastroenterology 1999年第3期5卷 241-244页

作者： Liu ZG Yang JH An hz Wang HY He FT Han ZY Han Y Wu HP xiao B Fan DM Fourth Mil Med Univ Dept Gastroenterol Xijing Hosp Xian 710032 Shaanxi Prov Peoples R China Harvard Univ Dept Cellular & Mol Biol Cambridge MA 02138 USA

AIM To obtain human and murine cDNAsencoding IFN--y inducible protein 10 (lP--10) andcytokine responsive gene--2 (Crg-2).METHODS The encoding genes of lP-10 and Crg2 were amplified by RT--PCR from Cultured humanfibroblast cells and Balb/ c mouse liver treatedby IFN-y and TNF-a, respectively, and clonedinto plasmids of PUC19 and PGEM3Zf(+ ).RESULTS The nucleotide sequences ot theamplified DNA were confirmed by endonucleasesdigestion and *** Recombinant lP-10/ crg--2 geneclones with 306 hp and 314 hp inserts wereestablished for further research on biologicalactivities and ligands of hiP-10/mCrg--2.

关键词： IP Nature Figure Cloning and identification of an angiostatic molecule IP-10/crg-2

Characteristics and efficiency of a new vibrating screen with a swing trace

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Particuology 2013年第5期11卷 601-606页

作者： Jianzhang xiao Xin Tong School of Mechanical Engineering and Automation Huaqiao University

A new vibration type and motion characteristics were proposed according to the principle of manual sie- ving. A mechanical model of the new motion was established and the characteristics of the new vibrating screen were analyzed to establish its equation of motion by using the vibration theory, to include as tech- nological parameters, amplitude, movement velocity, and throwing index through theoretical calculation. The efficiency of particle screening was studied at different values of frequency and swing declination angle. The discrete element method （DEM） was used to simulate the screening with the swing trace. The functional relationship between screening efficiency and the parameters was fitted with the least square method. The results show that high frequency and large swing angle are suitable for small particles, while small values are suitable for particles close to the aperture size. Compared to the linear vibration trace, both screening efficiency and processing capacity were effectively improved.

关键词： Motion characteristicSwing traceScreening efficiencySimulation

Methods to Improve the Long Distance Time-Varying Channel Transmission Performance of Expendable Profiler

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China Ocean Engineering 2019年第6期33卷 753-761页

作者： ZHENG Yu ZHANG xiao-yang WANG xiao-rui GUO Xing-xin LI Hong-zhi School of Life Sciences Tianjin Polytechnic UniversityTianjin 300387China Tianjin Key Laboratory of Photoelectric Detection Technology and System Tianjin Polytechnic UniversityTianjin 300387China National Marine Data and Information Service Tianjin 300171China National Ocean Technology Center Tianjin 300112China

To improve the transmission performance of XCTD channel, this paper proposes a method to measure directly and fit the channel transmission characteristics by using frequency sweeping method. Sinusoidal signals with a frequency range of 100 hz to 10 k hz and an interval of 100 hz are used to measure transmission characteristics of channels with lengths of 300 m, 800 m, 1300 m, and 1800 m. The correctness of the fitted channel characteristics by transmitting square wave, composite waves of different frequencies, and ASK modulation are verified. The results show that when the frequency of the signal is below 1500 hz, the channel has very little effect on the signal. The signal compensated for amplitude and phase at the receiver is not as good as the uncompensated ***, when the signal frequency is above 1500 hz, the channel distorts the signal. The quality of signal compensated for amplitude and phase at receiver is better than that of the uncompensated signal. Thus, we can select the appropriate frequency for XCTD system and the appropriate way to process the received signals. Signals below1500 hz can be directly used at the receiving end. Signals above 1500 hz are used after amplitude and phase compensation at the receiving end.

关键词： expandable conductivity temperature depth(XCTD)profiler time-varying channel modeling frequency sweeping method amplitude and phase compensation transmission performance

Combustion instability of pilot flame in a pilot bluff body stabilized combustor

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Chinese Journal of Aeronautics 2015年第6期28卷 1606-1615页

作者： Fu xiao Yang Fujiang Guo Zhihui National Key Laboratory of Science and Technology on Aero-Engine Aero-Thermodynamics School of Energy and Power Engineering Beihang University

Combustion instability of pilot flame has been investigated in a model pilot bluff body stabilized combustor by running the pilot flame only. The primary objectives are to investigate the pilot flame dynamics and to provide bases for the study of the interaction mechanisms between the pilot flame and the main flame. Dynamic pressures are measured by dynamic pressure transduc- ers. A high speed camera with CH＊ bandpass filter is used to capture the pilot flame dynamics. The proper orthogonal decomposition （POD） is used to further analyze the high speed images. With the increase of the pilot fuel mass flow rate, the pilot flame changes from stable to unstable state grad- ually. The combustion instability frequency is 136 hz when the pilot flame is unstable. Numerical simulation results show that the equivalence ratios in both the shear layer and the recirculation zone increase as the pilot fuel mass flow rate increases. The mechanism of the instability of the pilot flame can be attributed to the coupling between the second order acoustic mode and the unsteady heat release due to symmetric vortex shedding. These results illustrate that the pilot fuel mass flow rate has significant influences on the dynamic stability of the pilot flame.

关键词： Combustion instability High speed image Pilot flame Proper orthogonal decom-position (POD) Vortex shedding