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Protective effect of ginsenoside Rg1 on glutamate-induced lung injury

Acta Pharmacologica Sinica 2007年第3期28卷 392-397页

作者： Li SHEN~2 Jian-zhong HAS~2 Chen LI~2 Shao-jie YUE~3 Yong LIU~2 Xiao-qun QIN~2 Hui-jun LIU~2 Zi-qiang LUO~(3,4)2 Departments of Physiology,xiangya medical school and3 Neonatology,xiangya Hospital,Central South University,Changsha 410078,China Departments of Physiology Xiangya medical school Xiangya Hospital Central South University

Aim:To examine the possible protective effect of ginsenoside Rgl,an activecomponent of ginseng,on lung injury caused by glutamate in ***:Thelungs of mice receiving glutamate(0.5 g/kg)and/or ginsenoside Rg1(0.03 g/kg)via intraperitoneal administration were *** indexes of lung wet weight/body weight ratios(LW/BW),lung wet/dry weight ratios(W/D),heart rate(HR),and breathing rate(BR)were *** activity of nitric oxide synthase(NOS),xanthine oxidase(XOD),superoxide dismutase(SOD),catalase(CAT),thecontent of NO,and malondialdehyde in the lung homogenate were ***:Treatment with glutamate for 2 h increased LW/BW,W/D,HR,and *** changes were nearly abolished by pretreatment with ginsenoside Rgl for30 min before glutamate *** analysis of the lung homogenate demon-strated the protective effect as evidenced by the inhibition of NOS(12%)andXOD(50%)inactivity,the enhanced activity of SOD(20%)and CAT(25%).Conclusion:Ginsenoside Rgl has a potential protective role in lung diseasesassociated with glutamate toxicity.

关键词： ginsenoside Rgl glutamate acute lung injury nitrogen monoxide reactive oxygen species

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Effect of Apolipoprotein A-I on ATP Binding Cassette Transporter A1 Degradation and Cholesterol Efflux in THP-1 Macrophage-derived Foam Cells

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Acta Biochimica et Biophysica Sinica 2004年第3期36卷 218-226页

作者： Chao-Ke TANG1,2, Guo-Hua TANG1, Guang-Hui YI1, Zuo WANG1, Lu-Shan LIU1, Shuang WAN1, Zhong-Hua YUAN1, Xiu-Sheng HE1, Jun-Hao YANG1, Chang-Geng RUAN1, and Yong-Zong YANG1* 1Institute of Cardiovascular Disease of Nanhua University, Hengyang 421001, China 2xiangya medical school, Central Southern University, Changsha 410078, China Institute of Cardiovascular Disease of Nanhua University Hengyang 421001 China xiangya medical school Central Southern University Changsha 410078 China

Cholesterol-loaded macrophage foam cells are a central componentof atherosclerotic lesions. ATP binding cassette transporter A1 (ABCA1), the defective molecule inTangier disease, mediates the efflux of phospholipid and cholesterolfrom cells to apolipoprotein A-I (apoA-I), reversing foam cell formation. This study investigated the effect of apoA-I on ABCA1 degradation and cholesterol efflux in THP-1 macrophage- derived foam cells. After exposure of the cultured THP-1 macrophage-derived foam cells to apoA-I for different time, cholesterol efflux, ABCA1 mRNA and protein levels were determined by FJ-2107P type liquid scintillator, RT-PCR and Western blot, respectively. The mean ABCA1 fluorescence intensity on THP-1 macrophage-derived foam cells was detected by flow cytometry. Results showed that apoA-I markedly increased ABCA1-mediated cholesterol efflux from THP-1 macrophage-derived foam cells. This was accompanied by an increase in the content of ABCA1. ApoA-I did not alter ABCA1 mRNA abundance. Significantly, thiol protease inhibitors increased the level of ABCA1 protein and slowed its decay in THP-1 macrophage-derived foam cells, whereas none of the proteosome-specific inhibitor lactacystin, other protease inhibitors, or the lysosomal inhibitor NH4Cl showed such effects. The apoA-I-mediated cellular cholesterol efflux was enhanced by thiol protease inhibitors. Our results suggested that thiol protease inhibitors mightprovide an alternative way to upregulate ABCA1 protein. Thisstrategy is especially appealing since it may mimic the stabilizingeffect of the natural ligands apoA-I.

关键词： ATP binding cassette transporter A1 apoA-I flow cytometry cholesterol lipoproteins

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Genetic and epigenetic etiology of Parkinson diseases

Genetic and epigenetic etiology of Parkinson diseases

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2012年海内外华人神经科学会议

作者： Zhuo-Hua Zhang~* The State Key Laboratory of medical Genetics xiangya medical school Central South University Changsha Hunan China.

＜正＞Parkinson’s disease（PD） is the most common neurodegenerative movement *** pathological hallmarks of PD are loss of dopaminergic neurons in substantia nigra compacta（SNc） of midbrain and formation of intracellular aggregate Lowy bodies. Majority of PD patients are ***,about 10%PD cases are *** genetic studies of familial cases result in identification of causative mutations in multiple *** analysis of these PD linked genes has provided important insight into the disease pathogenic ***,the etiology of sporadic PD remains largely *** long-term research goal is to reveal the molecular mechanism of *** hypothesize that both genetic and environmental factors play important roles in PD pathogenesis and that the environmental factors regulate PD genesis and progression via at least partially epigenetic mechanism. Based on the hypothesis,our research focus includes to elucidate the pathophysiological function of PD linked genes and their pathogenic mutants and to identify the epigenetic changes associated with *** results suggest that PD-linked parkin,PINK1,and DJ-1 form a functional E3 ligase complex to promote ubiquitination and proteasomal degradation ofun/misfolded *** of the E3 ligase complex is regulated by nedd8 modification of both parkin and *** identify PD-associated epigenetic modification,we have performed genome-wide methylation sequencing of brain tissue DNAs from 5 PD patients and their matched *** reveal hypermethy lation of 234 genes in PD brain tissues over their *** 30%of these genes are related to *** vitro studies show that cells treated with sub-lethal dosage of PD neurotoxin MPP+ results in reduced expression of genes in neurogenesis pathway in addition to increased expression of immune related genes and cell death related genes. The study provides to our knowledge the first evidence of linking epigenetic

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Epigenetic regulation in Parkinson disease etiology

Epigenetic regulation in Parkinson disease etiology

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Cheng-Yuan TANG,Jie-Qiong TAN,Dan-Ling WANG,Zhuo-Hua ZHANG State Key Laboratory of medical Genetics,xiangya medical school,Central South University,Changsha,China

＜正＞It is well documented that both genetic and environmental factors play important roles in etiology of Parkinson disease（PD）.Genetic studies of familial cases identify mutations in more than 10 genes causing *** pathophysiological function of these genes remains largely ***,a number of pesticides and herbicides induce parkinsonism in both human and animal *** of them are shown to regulate PD associated genes. During the past decade,we have interested in roles of genes-environmental factors interaction in PD *** results suggest that PD-related environmental factors contribute to PD etiology likely via epigenetically regulation of PD associated *** this presentation,we will discuss in detail PD associated epigenetic modification.

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Mutation screening of the LINGO4 gene in Chinese Han patients with essential tremor

Mutation screening of the LINGO4 gene in Chinese Han patient...

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中国的遗传学研究——遗传学进步推动中国西部经济与社会发展——2011年中国遗传学会大会

作者： Hui Liang~1,Wen Zheng~1,Hongbo Xu~1,Jing Lei~1,Zhi Song~2,Xuhong Jiang~2,Zeshuai Zeng~(1,3),and Hao Deng~(1,2) 1 Center for Experimental Medicine,the Third xiangya Hospital,Central South University,Changsha,China 2 Department of Neurology,the Third xiangya Hospital,Central South University,Changsha,China 3 medical Technology,xiangya medical school,Central South University,Changsha,China.

Essential tremor(ET) is one of the most common neurological disorder with no disease-causing gene has been ***,genetic variants in two members of the leucine-rich repeat and lg domain containing nogo receptor-interacting protein genes(LINGO1 and LINGO2) were reported to be associated with ET susceptibility in certain *** investigate whether the LINGO4 gene(a homologous gene of the LINGO1 and the LINGO2 genes plays a role in ET susceptibility in Chinese Han population,we performed genetic analysis of coding region and flanking sequence of LINGO4 in 100 Chinese Han patients with essential *** variants were identified including an A>T transition(rs61746299),predicted to lead to the amino acid change at residue 444 from Thr to Ser,and a C>T transition(rs1521179),located 12 bp downstream from the coding region and did not affect splicing *** analysis was conducted with a total of 150 ET patients and 300 sex,age and ethnicity matched healthy controls. No significant differences in genotypic and allele distribution between patients and controls for rs61746299 and rs1521179(p = 0.531 and p = 0.867 for genotype distribution;p=1.000 and p= 0.844 for allele distribution) were observed,suggesting variants in the LINGO4 gene may play litter or no role in ET susceptibility.

关键词： essential tremor the LINGO4 gene variant susceptibility

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Downregulation of integrin β4 inhibit the antigen presentation ability on airway epithelial cells

Downregulation of integrin β4 inhibit the antigen presentat...

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中国生理学会第十一届张锡钧基金全国青年优秀生理学学术论文交流及评奖会议

作者： Chi Liu~1,Xiao-qun Qin~(1§),Hui-jun Liu~1,Yang Xiang~1,Ge Gao~2,Xiao-lin Zhu~1, (1.Physiology Department,xiangya medical school,Central South University, Changsha,Hunan,P.R.China. 2.Inspection Department,xiangya medical school,Central South University, Changsha,Hunan,P.R.China.)

The airway epithelial cells have been demonstrated as a kind of accessory antigen presentation cells(APC) to activate T cells which may play an important role in the development of allergic airway inflammation of *** the asthmatic airways,loss of expression of the adhesion molecule integrinβ4 and typical Th2 inflammation bias has been *** integrinβ4 also engaged in multiple signaling pathways,we studied whether disruption of integrinβ4 mediated cell adhesion may contribute to the adaptive immune response of epithelial cells:e.g.,antigen presentation,the activation and derivation of T *** silenced integrinβ4 expression in bronchial epithelial cells by effective small interference RNA(siRNA) and studied the effects of integrinβ4 silence on antigen presentation ability of airway epithelial cells.T cells proliferation and cytokine production were also investigated after co-culturing with integrinβ4 silence epithelial *** silencing of integrinβ4 resulted in impaired antigen presentation ***,our results demonstrated that down expression of integrinβ4 on asthma airway epithelial cells result in suppression of antigen presentation ability,subsequent inhibited Th1 cells proliferation and cytokine *** the Th1/Th2 ratio was also down ***,down regulation of integrinβ4 expression on airway epithelial cells could impair the antigen presentation ability of airway epithelial cell which further inhibits the Th1 inflammation differentiation in allergic asthma.

关键词： integrin β4 airway epithelial cell antigen presentation T cells

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Screening, Identification and Function Research on the Candidate Genes of Vaccine against Schistosomiasis

Screening, Identification and Function Research on the Candi...

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2005年寄生虫学国际学术研讨会暨中国动物学会寄生虫专业委员会第十次学术研讨会

作者： Liv Zhiyue Wang Shiping Zeng Shaohua Xu Shaorui Zhou Songhua Yu Junlong Dai Gan Xiao Xiaoqin Jiang Xiaoxin (Central South University, xiangya medical school Changsha 410078)

Aim Schistosomiasis is a severe world public-health problem, with more than 600 million people exposed to infection and nearly 200 million people infected. At the end of 2003, there were 110 counties from 7 provinces in China involved in the epidemic areas of schistosomiasis with a sharp increase of the patient number and the area of Oncomelania hupensis habitat recently. It’s necessary to find out effective vaccine molecules against this plague. A number of different schistosome antigens have been identified, about 15% of which are strongly protective and deemed promising, but they do not reach the level close to sterile immunity after vaccination with irradiated cercariae. The national schistosome vaccine unitive test carried out in 2000 showed the natural molecular vaccine (Vaccine No.1) constructed by Wang SP et al. was the best vaccine of 16 vaccines, with a protective immunity level close to that of the irradiated cercariae vaccine. Based on the results above-mentioned, we prepared the sera from rabbits vaccinated with irradiated cercariae (VRS) and the sera from pigs vaccinated with the natural molecular vaccine. Schistosoma japonicum (Sj) cercaria cDNA library was screened by the two sera and 5 novel genes were found. Further functional studies on the protective effects of the 2 from these novel genes were performed to value the potentiality of being the effective component of vaccine against schistosomiasis, and to lay a solid foundation for the construction and field-test of the vaccine in the future.

关键词： Schistosomajaponicum Vaccine Candidate gene cDNA library Function research

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Novel ATP7B mutations and found effects in Chinese Han families with Wilson's disease

Novel ATP7B mutations and found effects in Chinese Han famil...

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中国的遗传学研究——遗传学进步推动中国西部经济与社会发展——2011年中国遗传学会大会

作者： Hua-rong Yang~(1,2),Shao-juan Gu~(1,2),Yong Qi~1,Xiong Deng~1,Jing Lei~(1,2),Jing Li~3,Le Zhang~3,Hui Liang~1,Yi Guo~4,Xiao-liu Shi~5,Hao Deng~(1,2,*) 1 Center for Experimental Medicine,the Third xiangya Hospital,Central South University,Changsha,China 2 Department of Neurology,the Third xiangya Hospital,Central South University,Changsha,China 3 Department of Neurology,xiangya Hospital,Central South University,Changsha,China 4 Department of Physiology,xiangya medical school,Central South University,Changsha,China 5 Department of Gastroenterology,the Second xiangya Hospital,Central South University,Changsha,China

Wilson’s disease(WD) is an autosomal recessive disorder of copper metabolism and it is caused by mutations in the ATP7B *** investigate the ATP7B mutation in nine Chinese Han families with WD,direct genomic DNA sequencing was performed to identify the ATP7B *** different mutations,including 4 novel mutations(Vall45Phe,Gln388X, Thr498Ser and Gly837X) were identified in all 9 Chinese Han *** patients harbor homozygous or compound heterozygous ATP7B mutations,whereas heterozygous ATP7B mutation carriers were all free of any clinical *** analysis of different families with the same mutations revealed that founder effects for Arg778Leu,Pro992Leu and Ala1295Val mutations exist in these *** results may provide new insights into the cause and diagnosis of WD.

关键词： Wilson’s disease the mutation the ATP7B gene haplotype analysis

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Identification of four novel ATP7B mutations and found effects in Chinese Han families with Wilson's disease

Identification of four novel ATP7B mutations and found effec...

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中国的遗传学研究——遗传学进步推动中国西部经济与社会发展——2011年中国遗传学会大会

作者： Hua-rong Yang~(1,2),Shao-juan Gu~(1,2),Yong Qi~1,Xiong Deng~1,Jing Lei~(1,2),Jing Li~3,Le Zhang~3,Hui Liang~1,Yi Guo~4,Xiao-liu Shi~5,Hao Deng~(1,2,*) 1 Center for Experimental Medicine,the Third xiangya Hospital.Central South University,Changsha,China 2 Department of Neurology,the Third xiangya Hospital,Central South University,Changsha,China 3 Department of Neurology.xiangya Hospital.Central South University.Changsha.China 4 Department of Physiology,xiangya medical school,Central South University,Changsha.China 5 Department of Gastroenterology,the Second xiangya Hospital,Central South University,Changsha.China

Background/Aims:Wilson’s disease(WD) is an autosomal recessive disorder of copper metabolism and it is caused by mutations in the ATP7B gene. Methods:Direct genomic DNA sequencing was performed to identify the ATP7B mutation and haplotype analysis was conducted to evaluate founder effects. Results:Nine Chinese Han families with WD were screened and 11 different mutations,including 4 novel mutations(Val145Phe,Glu388X,Thr498Ser and Gly837X) were *** patients harbor homozygous or compound heterozygous ATP7B mutations,whereas heterozygous ATP7B mutation carriers were all free of any clinical *** analysis of different families with the same mutations revealed that founder effects for Arg778Leu,Pro992Leu and Ala1295Val mutations exist in these families. Conclusions:Eleven mutations including 4 novel mutations in the ATP7B gene were found to cause autosomal recessive WD in 9 Chinese Han *** effects for Arg778Leu. Pro992Leu and Ala1295Val mutations exist in different families with the same *** results may provide new insights into the cause and diagnosis of WD.

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降调节与口服避孕药联用的意义

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生殖医学杂志 2009年第A1期18卷 24-25页

作者：肖红梅中南大学湘雅医学院生殖与干细胞研究所中信湘雅生殖与遗传专科医院生殖中心长沙410078

将口服避孕药用于体外受精、胚胎移植（IVF—ET）助孕治疗中最初乃至今日仍作为调节月经周期，便于控制促性腺激素（gonadotrophin，Gn）的启动和取卵时间，达到有计划安排治疗工作的需要的目的之一。近十多年来，围绕降调节联合口服避... 详细信息

将口服避孕药用于体外受精、胚胎移植（IVF—ET）助孕治疗中最初乃至今日仍作为调节月经周期，便于控制促性腺激素（gonadotrophin，Gn）的启动和取卵时间，达到有计划安排治疗工作的需要的目的之一。近十多年来，围绕降调节联合口服避孕药（oral contraceptivepill，OCP）预处理用于避免使用促性腺激素释放激动剂（GnRH—a）后的卵泡囊肿形成、改善卵巢低反应患者卵巢反应性和降低卵巢高反应患者卵巢反应性以减少卵巢过度刺激综合征（OHSS）的发生开展多方面研究。

关键词：口服避孕药垂体降调节体外受精-胚胎移植

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