检索结果-南通市图书馆

Enriched endoplasmic reticulum-mitochondria interactions result in mitochondrial dysfunction and apoptosis in oocytes from obese mice

引用

Journal of Animal Science and Biotechnology 2018年第1期9卷 91-98页

作者： Lihong Zhao Tengfei Lu Lei Gao xiangwei fu Shien Zhu Yunpeng Hou State Key Laboratory of Agrobiotechnolocjy College of Biolocjical Sciences China Agricultural University Yuanmincjyuan west Rd 2 Bejing100193 China Key Laboratory of Animal Genetics Breeding and Reproduction College of Animal Science and Technology China Agricultural University Beijing China

Background： Maternal obesity alters oocytes and subsequent fetal metabolism. An increasing number of studies have shown that the endoplasmic reticulums（ER） or mitochondria have important effects on oocyte quality, but there has been no study of the effect of mitochondria-associated ER membranes（MAMs） on oocyte quality. The present study was designed to assess whether the level of MAM and MAM-related proteins were different in oocytes from obese and control ***： First, oocytes from mice with high-fat-diet（HFD）-induced obesity had higher levels（either greater numbers or a higher proportion for the same numbers） of MAM than oocytes from control mice. The abundance of MAM-related proteins in oocytes from obese mice was significantly greater at both the messenger RNA and protein levels, including inositol 1,4,5-trisphosphate receptor, type 1（IP3R1）, inositol 1,4,5-trisphosphate receptor,type 2（IP3 R2） and phosphofurin acidic cluster sorting protein 2（PACS-2）. further, there was an increase in mitochondrial Ca^（2＋）（[Ca^（2＋）]_m） which was associated with increased apoptosis and compromised cytoplasmic maturation in oocytes from obese mice. Down-regulation of MAM-related protein IP3R1 in oocytes from obese mice decreased [Ca^2＋]_mand apoptosis and improved cytoplasmic maturation but did not reduce the overal MAM level. However, down-regulating MAM-related protein PACS-2 in oocytes from obese mice did reduce the level of MAM and [Ca^（2＋）]_m, which decreased the rate of apoptosis and improved cytoplasmic maturation of oocytes from obese ***： It is possible that enriched MAM could increase [Ca^（2＋）]_m, and this increase has been found to be associated with increased apoptosis and compromised cytoplasmic maturation in oocytes from obese mice. This finding suggests a novel therapeutic target for obesity-induced oocyte defects.

关键词： Apoptosis IP3R1 MAM Mitochondrial Ca2+ Obesity Oocyte PACS-2

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

An efficient method for the sanitary vitrification of bovine oocytes in straws

引用

Journal of Animal Science and Biotechnology 2014年第4期5卷 399-405页

作者： Yanhua Zhou xiangwei fu Guangbin Zhou Baoyu Jia Yi Fang Yunpeng Hou Shien Zhu National Engineering Laboratory for Animal Breeding Key Laboratory of Animal geneticsBreeding and ReproductionMinistry of AgricultureCollege of Animal Science and TechnologyChina Agricultural University Institute of Animal Genetics and Breeding College of Animal Science and TechnologySichuan Agricultural University(Chengdu Campus) State Key Laboratory for Agrobiotechnology College of Biological SciencesChina Agricultural University

Background：At present,vitrification has been widely applied to humans,mice and farm *** improve the efficiency of vitrification in straw,bovine oocytes were used to test a new two-step vitrification method in this ***：When in vitro matured oocytes were exposed to 20%ethylene glycol（EG20） for 5 min and 40%ethylene glycol（EG40） for 30 s,followed by treatment with 30%glycerol（Gly30）,Gly40 or Gly50,a volume expansion was observed in Gly30 and Gly40 but not *** indicates that the intracellular osmotic pressure after a 30 s differs between EG40 and ranged between Gly40（approximately 5.6 mol/L） and Gly50（approximately 7.0 mol/L）.Since oocytes are in EG40 just for only a short period of time（30 s） and at a lower temperature（4℃）,we hypothesize that the main function of this step in to induce *** on these results,we omitted the EG40 step,before oocytes were pretreated in EG20 for 5 min,exposed to pre-cooled（4℃） Gly50,for 30 s,and then dipped into liquid *** warming,81.1%of the oocytes survived,and the surviving oocytes developed into cleavage stage embryos（63.5%） or blastocysts（20.0%） after parthenogenetic ***：These results demonstrate that in a two-step vitrification procedure,the permeability effect in the second step is not *** is possible that the second step is only required to provide adequate osmotic pressure to condense the intracellular concentration of CPAs to a level required for successful vitrification.

关键词： Bovine Cryopreservation Oocytes Straw Vitrification

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Cryopreservation of farm animal gametes and embryos:recent updates and progress

引用

Frontiers of Agricultural Science and Engineering 2019年第1期6卷 42-53页

作者： Zhengyuan HUANG Lei GAO Yunpeng HOU Shien ZHU xiangwei fu National Engineering Laboratory for Animal Breeding/Key Laboratory of Animal Genetics Breeding and Reproduction of Ministry of Agriculture/College of Animal Science and TechnologyChina Agricultural University State Key Laboratory of Agrobiotechnology/College of Biological Sciences China Agricultural University

Cryopreservation has undergone tremendous advances and is widely used in animal production based on decades of study of cellular permeability, freezability and empirical generalization. Several improvement are particularly important: the cryopreservation protocol has been continuously re?ned over the years to achieve greater reproductive performance; cryoprotective agents are more effective and less toxic than previously; there has been signi?cant innovation in advanced cryopreservation systems and carriers. Despite this, there are still problems that urgently require practical solutions, such as remedies for cryodamage and encouraging the use of frozen–thawed porcine sperm in pig production.

关键词： vitrification gametes embryo animal production cryoprotective agent freezability

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Antioxidant procyanidin B2 protects oocytes against cryoinjuries via mitochondria regulated cortical tension

引用

Journal of Animal Science and Biotechnology 2023年第1期14卷 166-187页

作者： Qingrui Zhuan Jun Li Xingzhu Du Luyao Zhang Lin Meng Yuwen Luo Dan Zhou Hongyu Liu Pengcheng Wan Yunpeng Hou xiangwei fu Key Laboratory of Animal Genetics Breeding and Reproduction of the Ministry of Agriculture and Rural AffairsKey Laboratory for Animal Genetic ImprovementCollege of Animal Science and TechnologyNational Engineering Laboratory for Animal BreedingChina Agricultural UniversityBeijingChina. Department of Reproductive Medicine Reproductive Medical CenterThe First Hospital of Hebei Medical UniversityShijiazhuangHebeiChina. State Key Laboratories of Agrobiotechnology College of Biological SciencesChina Agricultural UniversityBeijingChina. State Key Laboratory of Sheep Genetic Improvement and Healthy Breeding Institute of Animal Husbandry and Veterinary SciencesXinjiang Academy of Agricultural and Reclamation SciencesShihhotzeChina.

Background:Irreversible cryodamage caused by oocyte vitrification limited its wild application in female fertility *** were always used to antagonist the oxidative stress caused by ***,the comprehensive mechanism underlying the protective role of antioxidants has not been *** B2(PCB2)is a potent natural antioxidant and its functions in response to vitrification are still *** this study,the effects of PCB2 on vitrified-thawed oocytes and subsequent embryo development were explored,and the mechanisms underlying the protective role of PCB2 were systematically ***:Vitrification induced a marked decline in oocyte quality,while PCB2 could improve oocyte viability and further development after parthenogenetic activation.A subsequent study indicated that PCB2 effectively attenuated vitrification-induced oxidative stress,rescued mitochondrial dysfunction,and improved cell ***,PCB2 also acts as a cortical tension regulator apart from strong antioxidant *** cortical tension caused by PCB2 would maintain normal spindle morphology and promote migration,ensure correct meiosis progression and finally reduce the aneuploidy rate in vitrified *** study reveals that ATP biosynthesis plays a crucial role in cortical tension regulation,and PCB2 effectively increased the cortical tension through the electron transfer chain ***,PCB2 would elevate the cortical tension in embryo cells at morula and blastocyst stages and further improve blastocyst ***'s more,targeted metabolomics shows that PCB2 has a beneficial effect on blastocyst formation by mediating saccharides and amino acids ***:Antioxidant PCB2 exhibits multi-protective roles in response to vitrification stimuli through mitochondria-mediated cortical tension regulation.

关键词： Cortical tension Meiosis Mitochondria Oocytes Procyanidin B2 Vitrification

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effects of the TLR4 transgene on reproductive traits and DNA methylation pattern of oocytes in ewes

引用

Frontiers of Agricultural Science and Engineering 2014年第4期1卷 314-320页

作者： Yi FANG xiangwei fu Junjie LI Ming DU Baoyu JIA Jinlong ZHANG Xiaosheng ZHANG Shien ZHU National Engineering Laboratory for Animal Breeding and Key Laboratory of Animal Genetics Breeding and Reproduction of Ministry of AgricultureCollege of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China College of Animal Science and Technology Agricultural University of HebeiBaoding 071000China Animal Husbandry and Veterinary Research Institute of Tianjin Tianjin 300412China

This study was conducted to systematically assess the reproductive performance of transgenic TLR4 *** the TLR4 transgenic founders(F_(0))and their positive offspring(F_(1)),hematological and reproductive parameters and the global DNA methylation level in oocytes at various stages were *** values of the physiological and biochemical parameters determined from the blood samples did not differ significantly between the transgenic and wild-type ***,the transgenic ewes showed reproductive traits similar to the wildtype *** traits included characteristics of puberty,the estrus cycle,estrus duration,gestation,the pregnancy rate and the superovulation ***,no significant differences were found between transgenic and wild-type ewes in the DNA methylation level of the oocytes at various *** summary,the preliminary evidence presented in this paper demonstrates that the presence of the TLR4 transgene did not affect the reproductive performance in sheep.

关键词： TLR4 transgenic ewe safety assessment reproductive trait oocyte DNA methylation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

DNA Methylation Pattern in Pronuclear-Stage Mouse Embryos:Effect of Oocyte Vitrification

引用

Journal of Animal Science and Biotechnology 2011年第4期2卷 192-198页

作者： Ying Liang xiangwei fu Junjie Li Dianshuai Yuan Shien Zhu Key Laboratory of Animal Genetics Breeding and Reproduction Ministry of Agriculture and National Engineering Laboratory for Animal Breeding College of Animal Science and Technology China Agricultural University Beijing 100193 China

This study was conducted to investi- gate the pattern of DNA methylation in pronuclearstage mouse embryos derived from vitrified-warmed oocytes. Mouse oocytes at metaphase II （MII） stage of meiosis were allocated randomly into three groups： 1） untreated （control）; 2 ） exposed to vitrification solution without being plunged into liquid nitrogen （toxicity）; and 3 ） vitrified by open-pulled straw （OPS） method （vitrification）. Oocytes were fertilized in vitro （IVF）. The level of DNA methylation was examined at 8 hpf （hours post-fertilization） by immunofluorescence using an anti-5-methylcytosine （5-MeC） monoclonal antibody and fluorescein isothiocyanate （FITC）-conjugated goat anti-mouse IgG. After IVF, rates of 2-cell embryos （ 51.39% ） and blastocysts （35.82%） for vitrified-warmed oocytes were lower （P〈0.01） than that for control （70.83%, 47.82% ） or vitrification solution treated （64.80%, 46.29% ） oocytes. At 8 hpf, there were more （P 〈 0.05） pronuclear-stage oocytes in which syngamy of pronuclei had not occurred in the vitrifica- tion group. In addition, 5-MeC fluorescent intensities for the female pronucleus and zygote were lower in the vitrification group （P 〈0.01 ） compared to pronuclear-stage embryos in the control and toxicity groups. In conclusion, oocyte vitrification causes a reduction in global genomic methylation in the female pronucleus and zygote, resulting in delayed fusion of pronuclei and compromising the developmental potential of mouse zygotes and embryos.

关键词： DNA methylation mouse oocyte open-pulled straw vitrification pronucleus

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effect of maternal aging on the Nampt/FoxO3a axis of mice oocytes:the potential mechanism and related phenomena

Effect of maternal aging on the Nampt/FoxO3a axis of mice oo...

引用

中国畜牧兽医学会动物繁殖学分会第二十次学术研讨会

作者： Qingrui Zhuan Xingzhu Du Luyao Zhang Yunpeng Hou Jun Li Shien Zhu xiangwei fu China Agricultural University The First Hospital of Hebei Medical University

Introduction Maternal aging has been reported to impair the quality and decrease the developmental competence of ovulated *** phosphoribosyltransferase (Nampt) is a rate-limiting enzyme that catalyzes the production of nicotinamide mononucleotide (NMN) from *** subsequently converted to NAD,which is a critical component for cell energy metabolism and survival.

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Mitochondrial Ca2+ overload leads to mitochondrial oxidative stress and delayed meiotic resumption in mouse oocytes

Mitochondrial Ca2+ overload leads to mitochondrial oxidative...

引用

中国畜牧兽医学会动物繁殖学分会第二十次学术研讨会

作者： Luyao Zhang Zichuan Wang Lin Meng Qingrui Zhuan Yan Luo Shien Zhu xiangwei fu Yunpeng Hou China Agricultural University

Introduction Overweight or obese women seeking pregnancy is becoming increasingly *** maternal obesity gives rise to detrimental effects during *** evidence has shown that these abnormities are likely attributed to oocyte *** stress induces poor oocyte conditions,but whether mitochondrial calcium homeostasis plays a key role in oocyte status remains unresolved.

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

基于深度学习的合成孔径雷达图像去噪算法

引用

光学学报 2023年第6期43卷 188-198页

作者：付相为单慧琳吕宗奎王兴涛南京信息工程大学电子与信息工程学院江苏南京210044 无锡学院电子信息工程学院江苏无锡214105

针对合成孔径雷达在生成图像时产生的固有斑点噪声问题,提出了一种基于多尺度注意力级联卷积神经网络的去噪算法,用多尺度卷积网络与注意力机制来实现图像的特征提取,将级联网络结构作为主网络中的特征增强部分,并加入批量归一化来防止... 详细信息

针对合成孔径雷达在生成图像时产生的固有斑点噪声问题,提出了一种基于多尺度注意力级联卷积神经网络的去噪算法,用多尺度卷积网络与注意力机制来实现图像的特征提取,将级联网络结构作为主网络中的特征增强部分,并加入批量归一化来防止模型中出现过拟合。实验结果表明,所提算法相较于其他传统图像去噪算法,峰值信噪比、结构相似性分别平均提高了0.75 dB~14.45 dB和0.01~0.16,在图像熵上也优于其他算法,并且能较好地恢复图像中的细节信息。

关键词：图像处理合成孔径雷达卷积神经网络图像去噪多尺度注意力

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

性激素调控大脑性别分化的研究进展

引用

中国畜牧杂志 2024年第10期60卷 7-13页

作者：陈晶李俊侯云鹏傅祥伟福建农林大学动物科学学院福建福州350002 中国农业大学动物科学技术学院北京100193 河北医科大学第一医院生殖中心河北石家庄050000 中国农业大学生物学院北京100193

大脑结构的性别差异控制着不同性别的哺乳动物在成年时期表现出不同的性行为、社会行为以及内分泌调节等,雄性动物出生前后由睾丸产生的高水平雄激素是引起大脑性别分化的关键因素之一。然而,雄激素峰的具体产生和作用机制未完全阐明。... 详细信息

大脑结构的性别差异控制着不同性别的哺乳动物在成年时期表现出不同的性行为、社会行为以及内分泌调节等,雄性动物出生前后由睾丸产生的高水平雄激素是引起大脑性别分化的关键因素之一。然而,雄激素峰的具体产生和作用机制未完全阐明。近年来许多研究利用啮齿动物为模型,证明了雄激素峰上游被Kisspeptin所调控,下游通过转录水平、蛋白修饰以及免疫细胞等途径影响不同性别的大脑神经元和神经环路的发育。本文围绕雄激素峰上下游调控机制的研究进展进行综述,为家畜和人类逐年上升的繁殖疾病的可能机理研究提供参考。

关键词：大脑性别分化性激素表观修饰免疫细胞 Kisspeptin

来源：

维普期刊数据库博看期刊

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：