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Regulatory role of L-proline in fetal pig growth and intestinal epithelial cell proliferation

Animal Nutrition 2020年第4期6卷 438-446页

作者： Jing Wang Bi'e Tan Jianjun Li xiangfeng kong Minjie Tan Guoyao Wu Hunan International Joint Laboratory of Animal Intestinal Ecology and Health Laboratory of Animal Nutrition and Human HealthCollege of Life SciencesHunan Normal UniversityChangsha 410081China College of Animal Science and Technology Hunan Agricultural UniversityChangsha 410128China Laboratory of Animal Nutritional Physiology and Metabolic Process Key Laboratory of Agro-ecological Processes in Subtropical RegionNational Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry ProductionInstitute of Subtropical AgricultureChinese Academy of SciencesChangsha 410125China Department of Animal Science Texas A&M UniversityCollege StationTX 77843USA

L-proline(Pro)is a precursor of ornithine,which is converted into polyamines via ornithine decarboxylase(ODC).Polyamines plays a key role in the proliferation of intestinal epithelial *** study investigated the effect of Pro on polyamine metabolism and cell proliferation on porcine enterocytes in vivo and in ***-four Huanjiang mini-pigs were randomly assigned into 1 of 3 groups and fed a basal diet that contained 0.77%alanine(Ala,iso-nitrogenous control),1%Pro or 1%Pro+0.0167%α-difluoromethylornithine(DFMO)from d 15 to 70 of *** fetal body weight and number of fetuses per litter were determined,and the small and large intestines were obtained on d 70±1.78 of *** in vitro study was performed in intestinal porcine epithelial(IPEC-J2)cells cultured in Dulbecco’s modified Eagle medium-high glucose(DMEM-H)containing 0μmol/L Pro,400μmol/L Pro,or400μmol/L Pro+10 mmol/L DFMO for 4 *** results showed that maternal dietary supplementation with 1%Pro increased fetal weight;the protein and DNA concentrations of the fetal small intestine;and mRNA levels for potassium voltage-gated channel,shaker-related subfamily,member 1(Kv1.1)in the fetal small and large intestines(P<0.05).Supplementing Pro to either gilts or IPEC-J2 cells increased ODC protein abundances and polyamine concentrations in the fetal intestines and IPEC-J2 cells(P<0.05).In comparison with the Pro group,the combined administration of Pro and DFMO reduced the expression of ODC protein and spermine concentration in the fetal intestine,as well as the concentrations of putrescine,spermidine and spermine in IPEC-J2 cells(P<0.05).Meanwhile,the percentage of cells in the S-phase and the mRNA levels of proto-oncogenes c-fos and c-myc were increased in response to Pro supplementation,whereas depletion of cellular polyamines with DFMO increased tumor protein p53(p53)mRNA levels(P<0.05).Taken together,dietary supplementation with Pro improved fetal pig growth and intestinal epithelial cell

关键词： L-proline Polyamine metabolism Ornithine decarboxylase a-difluoromethylornithine Intestine Pig

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Dietary supplementation with betaine or glycine improves the carcass trait,meat quality and lipid metabolism of finishing mini-pigs

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Animal Nutrition 2021年第2期7卷 376-383页

作者： Yinzhao Zhong Zhaoming Yan Bo Song Changbing Zheng Yehui Duan xiangfeng kong JinPing Deng Fengna Li Guangdong Provincial Key Laboratory of Animal Nutrition Regulation South China Agricultural UniversityGuangzhou 510642China Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process Changsha 410125China Key Laboratory of Agro-ecological Processes in Subtropical Region Institute of Subtropical AgricultureChinese Academy of SciencesChangsha 410125China Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production Changsha 410125China National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production Changsha 410125China Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central Ministry of AgricultureChangsha 410125China University of Chinese Academy of Sciences Beijing 100039China College of Animal Science and Technology Hunan Agricultural UniversityChangsha 410128China

The objective of the study is to evaluate and compare the effects of betaine or glycine on carcass trait,meat quality and lipid metabolism of finishing Huan Jiang *** called trimethylglycine is a methyl derivative of glycine,but few researches were conducted to compare the impact of dietary betaine and glycine on *** hundred and forty-four Huan Jiang mini-pigs(body weight=10.55±0.15 kg;70 d)were randomly divided to 3 treatment groups(basal diet,glycine or betaine).Results indicated that dietary betaine increased the average daily gain(ADG)and final weight(P<0.05).Dietary glycine or betaine markedly reduced average backfat thickness(P<0.05)and heightened lean percentage(P<0.01)compared to the control ***,in comparison with the control group,betaine significantly improved the redness(a*)and tenderness(shear force)of the longissimus dorsi(LD)muscle(P<0.05),whereas glycine only raised the value of a*of the LD muscle(P<0.05).These results showed that diet supplemented with 0.25%betaine and equimolar amounts of glycine could regulate cascass trait and meat quality of finishing Huan Jiang mini-pigs,and the effect of betaine was superior to that of glycine.

关键词： Betaine Glycine Meat quality Lipid metabolism Huan jiang mini-pig

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SIZ1-Mediated SUMOylation of ROS1 Enhances Its Stability and Positively Regulates Active DNA Demethylation in Arabidopsis

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Molecular Plant 2020年第12期13卷 1816-1824页

作者： xiangfeng kong Yechun Hong Yi-Feng Hsu Huan Huang Xue Liu Zhe Song Jian-Kang Zhu Shanghai Center for Plant Stress Biology and Center for Excellence in Molecular Plant Sciences Chinese Academy of SciencesShanghai 200032People's Republic of China University of Chinese Academy of Sciences BeijingPeople's Republic of China Department of Horticulture and Landscape Architecture Purdue UniversityWest LafayetteIN 47907USA

The 5-methylcytosine DNA glycosylase/lyase REPRESSOR OF SILENCING 1(ROS1)-mediated active DNA demethylation is critical for shaping the genomic DNA methylation landscape in *** and how the stability of ROS1 may be regulated by post-translational modifications is *** a methylation-sensitive PCR(CHOP-PCR)-based forward genetic screen forArabidopsis DNA hyper-methyl-ation mutants,we identified the SUMO E3 ligase SIZ1 as a critical regulator of active DNA *** of SIZ1 leads to hyper-methylation at approximately 1000 genomic ***1 physically in-teracts with ROS1 and mediates the SUMOylation of *** SUMOylation of ROS1 is reduced in siz1 mutant *** with that in wild-type plants,the protein level of ROS1 is significantly decreased,whereas there is an increased level of ROS1 transcripts in siz1 mutant *** results suggest that SIZ1-mediated SUMOylation of ROS1 promotes its stability and positively regulates active DNA demethylation.

关键词： SIZ1 SUMOylatoin ROS1 active DNA demethylation Arabidopsis

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宫内发育迟缓对仔猪肠道微生物的影响及营养调控

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动物营养学报 2022年第10期34卷 6265-6270页

作者：孔祥峰余祖功刘洋中国科学院亚热带农业生态研究所亚热带农业生态过程重点实验室动物营养生理与代谢过程湖南省重点实验室长沙410125 南京农业大学动物医学院南京210095

肠道微生物的早期定植对动物机体免疫系统发育、营养物质代谢和正常生长发育具有重要作用。与正常出生体重仔猪相比,宫内发育迟缓(IUGR)仔猪的肠道微生物的早期定植、结构组成与代谢特征明显不同。本文总结了IUGR仔猪肠道微生物的定植... 详细信息

肠道微生物的早期定植对动物机体免疫系统发育、营养物质代谢和正常生长发育具有重要作用。与正常出生体重仔猪相比,宫内发育迟缓(IUGR)仔猪的肠道微生物的早期定植、结构组成与代谢特征明显不同。本文总结了IUGR仔猪肠道微生物的定植规律和代谢特征,以及饲粮干预(主要是益生菌、益生元、氨基酸、植物提取物等)对IUGR仔猪肠道微生态的调控作用及可能机制,为改善IUGR仔猪的肠道健康和生长发育提供依据。

关键词：宫内发育迟缓仔猪肠道微生物饲粮干预

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Precise genome editing without exogenous donor DNA via retron editing system in human cells

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Protein & Cell 2021年第11期12卷 899-902页

作者： xiangfeng kong Zikang Wang Renxia Zhang Xing Wang Yingsi Zhou Linyu Shi Hui Yang HUIGENE Therapeutics Inc. Shanghai 200131China Institute of Neuroscience State Key Laboratory of NeuroscienceKey Laboratory of Primate NeurobiologyCenter for Excellence in Brain Science and Intelligence TechnologyChinese Academy of SciencesShanghai 200031China College of Life Sciences University of Chinese Academy of SciencesBeijing 100049China Shanghai Center for Brain Science and Brain-Inspired Intelligence Technology Shanghai 201210China

Dear Editor,CRISPR-Cas9 mediated seamless genome editing can be achieved by incorporating donor DNA into the CRISPR-Cas9 target loci via homology-directed repair(HDR),albeit with relative low efficiency due to the inefficient delivery of exogenous DNA(Cox et al.,2015;Gao,2021).Retrons are bacterial phage-defense related operons composed of a specialized reverse transcriptase(RT)and a relevant non-coding RNA(ncRNA)which can be partially reverse tran-scribed by RT initiating at a conserved guanosine(G)residue to produce a multicopy single-stranded DNA(msDNA)(Yee et al.,1984;Millman et al.,2020).After being reverse transcribed,the msDNA is usually covalently teth-ered to the ncRNA through the 2',5'-phosphodiesterbond between the priming G in ncRNA and 5'end of msDNA(Dhundale et al.,1987).The reverse transcription process,of which the specialized RT recognizes the unique secondary structure of retron ncRNA,is highly specific(Hsu et al.,1989).Additionally,desired msDNA can be generated in vivo by replacing the dispensable region of retron ncRNA with desired sequences(Mirochnitchenko et al.,1994;Simon et al.,2019).Therefore,retrons are promising biological sources for in vivo generation of DNA donors for HDR-me-diated precise genome editing.

关键词： donor specialized editing

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宫内发育迟缓仔猪小肠形态和屏障功能相关基因的表达特征

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动物营养学报 2022年第2期34卷 1240-1246页

作者：唐武张旺宏孔祥峰中国科学院亚热带农业生态研究所动物营养生理与代谢过程湖南省重点实验室长沙410125 中国科学院大学北京100049

为了研究宫内发育迟缓(IUGR)仔猪小肠形态和屏障功能相关基因的表达特征,选取24窝"长白×大白"杂交新生仔猪,每窝选取1头IUGR仔猪和1头正常出生体重(NBW)仔猪,分别于7、21和28日龄屠宰8头IUGR仔猪和8头NBW仔猪,采集小肠样品进行分析。... 详细信息

为了研究宫内发育迟缓(IUGR)仔猪小肠形态和屏障功能相关基因的表达特征,选取24窝"长白×大白"杂交新生仔猪,每窝选取1头IUGR仔猪和1头正常出生体重(NBW)仔猪,分别于7、21和28日龄屠宰8头IUGR仔猪和8头NBW仔猪,采集小肠样品进行分析。结果表明:1)与NBW仔猪相比,IUGR仔猪7日龄时空肠绒毛高度、21日龄时空肠和回肠绒毛高度显著降低(P<0.05),28日龄时空肠隐窝深度显著提高(P<0.05)。2)与NBW仔猪相比,IUGR仔猪7日龄时空肠黏膜闭锁小带蛋白-1(ZO-1)、封闭蛋白-1(claudin-1)、闭合蛋白(occludin)、白细胞介素-4(IL-4)和白细胞介素-8以及21日龄时空肠黏膜ZO-1和occludin、回肠黏膜ZO-1的mRNA相对表达量显著下调(P<0.05);28日龄时空肠黏膜肿瘤坏死因子-α和回肠黏膜白细胞介素-1β的mRNA相对表达量显著上调(P<0.05),回肠黏膜IL-4的mRNA相对表达量显著下调(P<0.05)。综上所述,IUGR仔猪的空肠和回肠形态受损,紧密连接蛋白基因表达下调,免疫细胞因子表达异常,进而影响小肠屏障功能。

关键词：宫内发育迟缓仔猪肠道形态紧密连接蛋白免疫细胞因子

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An engineered xCas12i with high activity,high specificity,and broad PAM range

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Protein & Cell 2023年第7期14卷 540-545页

作者： Hainan Zhang xiangfeng kong Mingxing Xue Jing Hu Zikang Wang Yinghui Wei Haoqiang Wang Jingxing Zhou Weihong Zhang Mengqiu Xu Xiaowen Shen Fengcai Yin Zhiyuan Ai Guangyan Huang Junhui Xia Xueqiong Song Hengbin Li Yuan Yuan Jinhui Li Na Zhong Meiling Zhang Yingsi Zhou Hui Yang HuiEdit Therapeutics Co. Ltd.Shanghai 200120China HuiGene Therapeutics Co. Ltd.Shanghai 200120China Center for Reproductive Medicine International Peace Maternity and Child Health HospitalInnovative Research Team of High-level Local Universities in ShanghaiSchool of MedicineShanghai Jiao Tong UniversityShanghai 200030China

Dear Editor,The clustered regularly interspaced short palindromic repeats-Cas(CRISPR-Cas)systems,including type II Cas9 and type V Cas12 systems,which serve in the adaptive immunity of prokaryotes against viruses,have been developed into genome-editing tools(Anzalone et al.,2020;Doudna,2020).Compared with type II systems,the type V systems including V-A to V-K showed more functional diversity(Yan et al.,2019).Amongst them,Cas12i has a relatively smaller size(1,033-1,093 aa),compared to SpCas9 and Cas12a,and has a 5'-TTN protospacer adjacent motif(PAM)preference(Yan et al.,2019).

关键词： high specificity

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Effects of dietary protein/energy ratio on growth performance,carcass trait,meat quality,and plasma metabolites in pigs of different genotypes

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Journal of Animal Science and Biotechnology 2015年第4期6卷 435-444页

作者： Yingying Liu xiangfeng kong Guoli Jiang Bi'e Tan Jinping Deng Xiaojian Yang Fengna Li Xia Xiong Yulong Yin Key Laboratory of Agro-ecological Processes in Subtropical Region Hunan Provincial Engineering Research Center of Healthy Livestock and Poultryand Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-CentralMinistry of AgricultureInstitute of Subtropical AgricultureChinese Academy of Sciences Hunan Animal Science and Veterinary Medicine Research Institute University of Chinese Academy of Sciences College of Animal Science and Technology Hunan Agricultural University Southern Research and Outreach Center University of Minnesota

Background： The protein/energy ratio is important for the production performance and utilization of available feed resources by animals. Increased protein consumption by mammals leads to elevated feed costs and increased nitrogen release into the environment. This study aimed to evaluate the effects of dietary protein/energy ratio on the growth performance, carcass traits, meat quality, and plasma metabolites of pigs of different genotypes. Methods： Bama mini-pigs and Landrace pigs were randomly assigned to two dietary treatment groups （Chinese conventional diet with low protein/energy ratio or National Research Council diet with high protein/energy ratio; n = 24 per treatment） in a 2 x 2 factorial arrangement. Blood and muscle samples were collected at the end of the nursery, growing, and finishing phases. Results： We observed significant interactions （P 〈 0.05） between breed and diet for total fat percentage, intramuscular fat （IMF） content, protein content in biceps femoris （BF） muscle, and plasma urea nitrogen （UN） concentration in the nursery phase; for average daily gain （ADG）, average daily feed intake （ADFI）, dry matter, IMF content in psoas major （PM） muscle, and plasma total protein and albumin concentrations in the growing phase; and for drip loss and plasma UN concentration in the finishing phase. Breed influenced （P 〈 0.05） growth performance, carcass traits, and meat quality, but not plasma metabolites. Throughout the trial, Landrace pigs showed significantly higher （P 〈 0.0_5） ADG, ADFI, dressing percentage, lean mass rate, and loin-eye area than did Bama mini-pigs, but significantly lower （P 〈 0.0.5） feed/gain ratio, fat percentage, backfat thickness, and IMF content. Dietary protein/energy ratio influenced the pH value, chemical composition of BF and PM muscles, and plasma activities of glutamic-pyruvic transaminase and gamma-glutamyl transpeptidase, and plasma concentration of UN. Conclusions： Compared with Landrace pigs, Bama mini-pigs showed slow

关键词： Dietary protein/energy ratio Growth performance Meat quality Mini-pig Plasma metabolites

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Developmental changes in intercellular junctions and Kv channels in the intestine of piglets during the suckling and post-weaning periods

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Journal of Animal Science and Biotechnology 2016年第2期7卷 228-237页

作者： Jing Wang Liming Zeng Bie Tan Guangran Li Bo Huang Xia Xiong Fengna Li xiangfeng kong Gang Liu Yulong Yin Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production Key Laboratory of Agro-ecological Processes in Subtropical Region institute of Subtropical AgricultureChinese Academy of SciencesChangshaHunan 410125China University of the ChineseAcademy of Sciences Beijing 10008China. Science College of Jiangxi Agricultural University NanchangJiangxi 330045China. Hunan Collaborative Innovation Center for Utilization of Botanical Functional Ingredients Hunan Collaborative Innovation Center of Animal Production SafetyChangshaHunan 410000China.

Background： The intestinal epithelium is an important barrier that depends on a complex mixture of proteins and these proteins comprise different intercellular junctions. The purpose of this study was to investigate the postnatal and developmental changes in morphology, intercellular junctions and voltage-gated potassium（Kv） channels in the intestine of piglets during the suckling and post-weaning ***： Samples of the small intestine were obtained from 1-, 7-, 14-, and 21-d-old suckling piglets and piglets on d 1, 3, 5, and 7 after weaning at 14 d of age. The results showed that the percentage of proliferating cell nuclear antigen（PCNA）-positive cells and alkaline phosphatase（AKP） activity, as well as the abundances of E-cadherin,occludin, and Kv1.5 m RNA and claudin-1, claudin-3, and occludin protein in the jejunum were increased from d 1to d 21 during the suckling period（P 〈 0.05）. Weaning induced decreases in the percentage of PCNA-positive cells,AKP activity and the abundances of E-cadherin, occludin and zonula occludens（ZO）-1 m RNA or protein in the jejunum on d 1, 3 and 5 post-weaning（P 〈 0.05）. There were lower abundances of E-cadherin, occludin and ZO-1m RNA as well as claudin-1, claudin-3 and ZO-1 protein in the jejunum of weanling piglets than in 21-d-old suckling piglets（P 〈 0.05）. The abundances of E-cadherin, occludin, ZO-1 and integrin m RNA were positively related to the percentage of PCNA-positive ***： Weaning at 14 d of age induced damage to the intestinal morphology and barrier. While there was an adaptive restoration on d 7 post-weaning, the measured values did not return to the pre-weaning levels, which reflected the impairment of intercellular junctions and Kv channels.

关键词： Intercellular junctions Kv channels Small intestine Piglets Weaning

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Myokine interleukin-15 expression profile is different in suckling and weaning piglets

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Animal Nutrition 2015年第1期1卷 30-35页

作者： Yehui Duan Fengna Li Bie Tan Binbin Lin xiangfeng kong Yinghui Li Yulong Yin Key Laboratory of Agro-ecological Processes in Subtropical Region Hunan Provincial Engineering Research Center of Healthy Livestock and Poultryand Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central Ministry of Agriculture Institute of Subtropical Agriculture Chinese Academy of Sciences University of Chinese Academy of Sciences College of Animal Science and Technology Hunan Agricultural University

Interleukin-15(IL-15) is a cytokine highly expressed in skeletal muscle. The objective of the present study was to investigate the development of muscle IL-15 expression in suckling piglets and in early weaning piglets(day 14) at each level, that is, mRNA, protein, and secretion. Eight litters(eight piglets per litter) of newborn healthy piglets(Large × White × Landrace) with a similar initial weight(1618.0 ± 140.1 g) were chosen and divided into two groups, Group one used suckling piglets that were killed, respectively, at days 1,7,14,21, and group two used early(day 14) weaning piglets that were killed respectively, at days 15, 17, 19, 21. In group one, IL-15 gene expression levels increased significantly(P 0.05)among piglets at other ages. These findings indicated that increased IL-15 mRNA expression did not result in a corresponding increase of its protein expression. In group two, which used early weaning piglets from days 15-19, IL-15 mRNA and protein expression levels increased constantly(P 0.05) compared with suckling piglets at day 14 of age. However, IL-15 protein expression levels in early weaning piglets at day 21 of age dropped significantly(P < 0.05) to the levels as suckling piglets at day 21 of age, while body weight increased(P < 0.05) markedly to the levels as suckling piglets at day 21 of age. In both groups, the serum IL-15 levels of piglets decreased significantly(P < 0.01) over time. Taken together, our results indicate that IL-15 expression differs in suckling piglets and in weaning piglets. It is speculated that IL-15 may play an important role in counteracting the effects of early weaning stress.

关键词： Interleukin-15 Piglets Weaning stress Inflammation

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