An engineered xCas12i with high activity,high specificity,and broad PAM range

作     者：Hainan Zhang Xiangfeng Kong Mingxing Xue Jing Hu Zikang Wang Yinghui Wei Haoqiang Wang Jingxing Zhou Weihong Zhang Mengqiu Xu Xiaowen Shen Fengcai Yin Zhiyuan Ai Guangyan Huang Junhui Xia Xueqiong Song Hengbin Li Yuan Yuan Jinhui Li Na Zhong Meiling Zhang Yingsi Zhou Hui Yang Hainan Zhang;Xiangfeng Kong;Mingxing Xue;Jing Hu;Zikang Wang;Yinghui Wei;Haoqiang Wang;Jingxing Zhou;Weihong Zhang;Mengqiu Xu;Xiaowen Shen;Fengcai Yin;Zhiyuan Ai;Guangyan Huang;Junhui Xia;Xueqiong Song;Hengbin Li;Yuan Yuan;Jinhui Li;Na Zhong;Meiling Zhang;Yingsi Zhou;Hui Yang

作者机构：HuiEdit Therapeutics Co.Ltd.Shanghai 200120China HuiGene Therapeutics Co.Ltd.Shanghai 200120China Center for Reproductive MedicineInternational Peace Maternity and Child Health HospitalInnovative Research Team of High-level Local Universities in ShanghaiSchool of MedicineShanghai Jiao Tong UniversityShanghai 200030China 

出 版 物：《Protein & Cell》 (蛋白质与细胞（英文版）)

年 卷 期：2023年第14卷第7期

页      面：540-545页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071009[理学-细胞生物学] 09[农学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：HUIGENE Therapeutics Inc 

主　　题：high specificity 

摘      要：Dear Editor,The clustered regularly interspaced short palindromic repeats-Cas(CRISPR-Cas)systems,including type II Cas9 and type V Cas12 systems,which serve in the adaptive immunity of prokaryotes against viruses,have been developed into genome-editing tools(Anzalone et al.,2020;Doudna,2020).Compared with type II systems,the type V systems including V-A to V-K showed more functional diversity(Yan et al.,2019).Amongst them,Cas12i has a relatively smaller size(1,033-1,093 aa),compared to SpCas9 and Cas12a,and has a 5 -TTN protospacer adjacent motif(PAM)preference(Yan et al.,2019).