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Development of wet adhesion of honeybee arolium incorporated polygonal structure with three-phase composite interfaces

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Friction 2024年第2期12卷 215-230页

作者： Lulu LIANG Jieliang ZHAO Qun NIU Li YU xiangbing wu Wenzhong WANG Shaoze YAN Zhenglei YU School of Mechanical Engineering Beijing Institute of TechnologyBeijing 100081China Division of Intelligent and Biomechanical Systems State Key Laboratory of TribologyDepartment of Mechanical EngineeringTsinghua UniversityBeijing 100084China Key of Bionic Engineering Ministry of EducationJilin UniversityChangchun 130022China

Inspired by the dynamic wet adhesive systems in nature,various artificial adhesive surfaces have been developed but still face different ***,the theoretical mechanics of wet adhesives has never been sufficiently ***,we develop a novel adhesive mechanism for governing wet adhesion and investigate the biological models of honeybee arolium for reproducing the natural wet adhesive ***-nano structures of honeybee arolium and arolium-prints were observed by Cryogenic scanning electron microscopy(Cryo-SEM),and the air pockets were found in the contact interface ***,the adhesive models with a three-phase composite interface(including air pockets,liquid secretion,and hexagonal frames of arolium),were formed to analyze the wet adhesion of honeybee *** results of theoretical calculations and experiments indicated an enhanced adhesive mechanism of the honeybee by liquid self-sucking effects and air-embolism *** these effects,normal and shear adhesion can be adjusted by controlling the proportion of liquid secretion and air pockets in the contact ***,the air-embolism effects contribute to the optimal coupling of smaller normal adhesion with greater shear adhesion,which is beneficial for the high stride frequency of *** works can provide a fresh perspective on the development of bio-inspired wet adhesive surfaces.

关键词： honeybee arolium air pockets wet adhesion a three-phase interface self-sucking effects air-embolism effects

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Chondroitinase ABC combined with Schwann cell transplantation enhances restoration of neural connection and functional recovery following acute and chronic spinal cord injury

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Neural Regeneration Research 2025年第5期20卷 1467-1482页

作者： Wenrui Qu xiangbing wu Wei wu Ying Wang Yan Sun Lingxiao Deng Melissa Walker Chen Chen Heqiao Dai Qi Han Ying Ding Yongzhi Xia George Smith Rui Li Nai-Kui Liu Xiao-Ming Xu Spinal Cord and Brain Injury Research Group Stark Neurosciences Research InstituteDepartment of Neurological SurgeryIndiana University School of MedicineIndianapolisINUSA Department of Hand Surgery the Second Hospital of Jilin UniversityChangchunJilin ProvinceChina Shriners Hospitals Pediatric Research Center Temple University School of MedicinePhiladelphiaPAUSA Department of Anatomy&Cell Biology Indiana University School of MedicineIndianapolisINUSA

Schwann cell transplantation is considered one of the most promising cell-based therapy to repair injured spinal cord due to its unique growth-promoting and myelin-forming properties.A the Food and Drug Administration-approved Phase I clinical trial has been conducted to evaluate the safety of transplanted human autologous Schwann cells to treat patients with spinal cord injury.A major challenge for Schwann cell transplantation is that grafted Schwann cells are confined within the lesion cavity,and they do not migrate into the host environment due to the inhibitory barrier formed by injury-induced glial scar,thus limiting axonal reentry into the host spinal *** we introduce a combinatorial strategy by suppressing the inhibitory extracellular environment with injection of lentivirus-mediated transfection of chondroitinase ABC gene at the rostral and caudal borders of the lesion site and simultaneously leveraging the repair capacity of transplanted Schwann cells in adult rats following a mid-thoracic contusive spinal cord *** report that when the glial scar was degraded by chondroitinase ABC at the rostral and caudal lesion borders,Schwann cells migrated for considerable distances in both rostral and caudal *** Schwann cell migration led to enhanced axonal regrowth,including the serotonergic and dopaminergic axons originating from supraspinal regions,and promoted recovery of locomotor and urinary bladder ***,the Schwann cell survival and axonal regrowth persisted up to 6 months after the injury,even when treatment was delayed for 3 months to mimic chronic spinal cord *** findings collectively show promising evidence for a combinatorial strategy with chondroitinase ABC and Schwann cells in promoting remodeling and recovery of function following spinal cord injury.

关键词： axonal regrowth bladder function chondroitinase ABC functional recovery glial scar lentivirus migration Schwann cell spinal cord injury transplantation

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Rho A/Rho kinase in spinal cord injury

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Neural Regeneration Research 2016年第1期11卷 23-27页

作者： xiangbing wu Xiao-ming Xu Spinal Cord and Brain Injury Research Group Stark Neurosciences Research Institute Indiana University School of Medicine Department of Neurological Surgery Indiana University School of Medicine Goodman Campbell Brain and Spine Indiana University School of Medicine Department of Anatomy & Cell Biology Indiana University School of Medicine

A spinal cord injury refers to an injury to the spinal cord that is caused by a trauma instead of diseases. Spinal cord injury includes a primary mechanical injury and a much more complex secondary injury process involving inflammation, oxidation, excitotoxicity, and cell death. During the secondary injury, many signal pathways are activated and play important roles in mediating the pathogenesis of spinal cord injury. Among them, the Rho A/Rho kinase pathway plays a particular role in mediating spinal degeneration and regeneration. In this review, we will discuss the role and mechanism of Rho A/Rho kinase-mediated spinal cord pathogenesis, as well as the potential of targeting Rho A/Rho kinase as a strategy for promoting both neuroprotection and axonal regeneration.

关键词： Rho A Rho kinase inflammation cell death degeneration regeneration spinal cord injury

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口腔颌面部损伤患者自我认同感与病耻感及美学修复倾向的相关性分析

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中华全科医学 2024年第1期22卷 46-49页

作者：庄燕阚卫娟吴祥冰南京医科大学附属无锡人民医院口腔科江苏无锡214000

目的探讨口腔颌面损伤患者自我认同感与病耻感及美学修复倾向的关系,旨在使口腔颌面损伤患者对康复效果了解更深入,并为制定更有效的临床干预和支持措施提供依据。方法选取2020年1月—2022年12月南京医科大学附属无锡人民医院收治的16... 详细信息

目的探讨口腔颌面损伤患者自我认同感与病耻感及美学修复倾向的关系,旨在使口腔颌面损伤患者对康复效果了解更深入,并为制定更有效的临床干预和支持措施提供依据。方法选取2020年1月—2022年12月南京医科大学附属无锡人民医院收治的160例口腔颌面部损伤患者,采用自我认同感量表、社会影响量表(评价病耻感)、美学修复倾向量表对患者进行调查,采用Pearson相关系数法分析自我认同感、病耻感、美容修复倾向之间的关系,通过构建结构方程模型分析自我认同感在病耻感与美学修复倾向之间的中介作用。结果口腔颌面部损伤患者自我认同感评分为(50.47±6.80)分,病耻感评分为(71.33±5.96)分,美学修复倾向评分为(45.24±5.38)分。自我认同感评分与病耻感评分呈负相关关系(r=-0.472,P=0.016),自我认同感评分与美学修复倾向评分呈正相关关系(r=0.517,P=0.008),病耻感评分与美学修复倾向评分呈正相关关系(r=0.490,P=0.012)。病耻感对美学修复倾向存在明显预测力(t=5.817,P<0.001),病耻感对自我认同感存在明显的预测力(t=-4.263,P<0.001),自我认同感在病耻感与美学修复倾向之间有部分中介作用(t=3.317,P<0.001)。结论口腔颌面损伤患者自我认同感、病耻感、美学修复倾向之间存在密切关系,自我认同感在病耻感与美学修复倾向之间有一定的中介作用。

关键词：口腔颌面损伤自我认同感病耻感美学修复倾向中介效应

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All-trans retinoic acid alleviates transmissible gastroenteritis virus-induced intestinal inflammation and barrier dysfunction in weaned piglets

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Journal of Animal Science and Biotechnology 2024年第3期15卷 1131-1144页

作者： Junning Pu Daiwen Chen Gang Tian Jun He Ping Zheng Zhiqing Huang xiangbing Mao Jie Yu Yuheng Luo Junqiu Luo Hui Yan Aimin wu Bing Yu Institute of Animal Nutrition Sichuan Agricultural UniversityChengdu 611130SichuanPeople’s Republic of China Key Laboratory of Animal Disease-Resistance Nutrition Ministry of EducationMinistry of Agriculture and Rural AffairsKey Laboratory of Sichuan Province611130 ChengduSichuanPeople’s Republic of China

Background Transmissible gastroenteritis virus(TGEV)is one of the main pathogens causing severe diarrhea of *** pathogenesis of TGEV is closely related to intestinal ***-trans retinoic acid(ATRA)is the main active metabolite of vitamin A,which has immunomodulatory and anti-inflammatory ***,it is unclear whether ATRA can alleviate TGEV-induced intestinal inflammation and barrier dysfunction in *** study aimed to investigate the effects of ATRA on growth performance,diarrhea,intestinal inflammation and intesti-nal barrier integrity of TGEV-challenged *** In a 19-d study,32 weaned piglets were randomly divided into 4 treatments:Control group(basal diet),TGEV group(basal diet+TGEV challenge),TGEV+ATRA5 group(basal diet+5 mg/d ATRA+TGEV challenge)and TGEV+ATRA15 group(basal diet+15 mg/d ATRA+TGEV challenge).On d 14,piglets were orally administered TGEV or the sterile *** Feeding piglets with 5 and 15 mg/d ATRA alleviated the growth inhibition and diarrhea induced by TGEV(P<0.05).Feeding piglets with 5 and 15 mg/d ATRA also inhibited the increase of serum diamine oxidase(DAO)activ-ity and the decrease of occludin and claudin-1 protein levels in jejunal mucosa induced by TGEV,and maintained intestinal barrier integrity(P<0.05).Meanwhile,5 mg/d ATRA feeding increased the sucrase activity and the expres-sions of nutrient transporter related genes(GLUT2 and SLC7A1)in jejunal mucosa of TGEV-challenged piglets(P<0.05).Furthermore,5 mg/d ATRA feeding attenuated TGEV-induced intestinal inflammatory response by inhibit-ing the release of interleukin(IL)-1β,IL-8 and tumor necrosis factor-α(TNF-α),and promoting the secretion of IL-10 and secretory immunoglobulin A(sIgA)(P<0.05).Feeding 5 mg/d ATRA also down-regulated the expressions of Toll-like receptors and RIG-I like receptors signaling pathway related genes(TLR3,TLR4,RIG-I,MyD88,TRIF and MAVS)and the phosphorylation level of nuclear factor-κB-p65(NF-κB p65),and

关键词： All-trans retinoic acid Inflammation Intestinal barrier Piglets Transmissible gastroenteritis virus

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Effect of Exogenous bFGF on the Proliferation of Human Adenoid Cystic Carcinoma ACC-2 Cells

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2008年第2期28卷 227-229页

作者：丁蕾朱声荣谢三祥吴祥冰 Department of Stomatology Tongji HospitalTongji Medical CollegeHuazhong University of Science and Technology Wuhan 430030 China

To observe the effects of basic fibroblast growth factor （bFGF） on human adenoid cystic carcinoma ACC-2 cell line proliferation and ERK, cyclin D1/p21^waf/cip1 signaling pathways, human adenoid cystic carcinoma cells （ACC-2） were cultured and the influence of bFGF of different concentrations on cell proliferation was determined by MTT. Protein was detected by immuno-precipitation and ERK activity by using ERK agent kit. p-ERK1/2 and down-stream cyclin D1, p21^waf/cip1 expression were detected by Western blotting and the interfering role of mitogen protein-activated kinase （MEK） suppressor U0126 in the afore-mentioned indicators was examined. MTT demonstrated ACC-2 cell proliferation was substantially enhanced by bFGF, immuo-precipitation displayed ERK activity was up-regulated by bFGF, and immuno-imprinting also showed p-ERK1/2, cyclin D1 expression was greatly enhanced and p21^waf/cip1 expression was inhibited by bFGE U0126 suppressed the effect of bFGF. It is concluded that bFGF can promote the proliferation of human adenoid cystic carcinoma ACC-2 cells, and its pathways are associated with the up-regulated activity and expression of p-ERK1/2, inhibited p21waf/cip1 expression and enhanced cyclin D1 expression.

关键词： basic fibroblast growth factor adenoid cystic carcinoma extracellular signal-regulated kinase cell cycle phase protein D1

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Dysregulated miR-363 affects head and neck cancer invasion and metastasis by targeting podoplanin

Dysregulated miR-363 affects head and neck cancer invasion a...

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第八届全国医学生物化学与分子生物学第五届全国临床应用生物化学与分子生物学2013华东六省一市生物化学与分子生物学联合学术研讨会

作者： Qiang Sun Jianjun Zhang Wei Cao Xu Wang Qin Xu Ming Yan xiangbing wu Wantao Chen Department of Oral and Maxillofacial-Head & Neck Oncology Ninth People's HospitalShanghai Jiao Tong University School of Medicine Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology

Head and neck squamous cell carcinoma(HNSCC) is characterised by an elevated capacity for tumour invasion and lymph node metastasis and the cause remains to be *** studies suggest that microRNAs can regulate the evolution of malignant behaviours by regulating multiple target *** this study,we have first confirmed that miR-363 is down-regulated in HNSCC tissues with lymph node metastasis and cell lines with highly invasive *** used bioinformatics,cellular and molecular methods to predict and prove that miR-363 directly targeted to podoplanin(PDPN) and caused up-regulation of PDPN in *** assay showed that DNA promoter methylation was involved in silencing the miR-363 in ***,we provided evidence to demonstrate that PDPN dysregulation caused by down-regulation of miR-363 contributes to HNSCC invasion and *** data reveal a key role of miR-363-PDPN in HNSCC metastasis and support biological and clinical links between miR-363-PDPN and HNSCC.

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Bmi-1基因在不同预后口腔白斑中的表达及其临床意义

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中华医学杂志 2022年第11期102卷 787-794页

作者：吴祥冰王荣艳龚中坚朱金晓朱声荣南京医科大学附属无锡人民医院口腔科无锡214023 南京医科大学附属无锡儿童医院口腔科无锡214023 华中科技大学同济医学院附属同济医院口腔医学中心武汉430030

目的:检测Bmi-1基因在口腔白斑细胞和组织中的表达,分析其在口腔白斑恶性转化中的作用及临床意义。方法:免疫组化法检测南京医科大学附属无锡人民医院口腔科和华中科技大学同济医学院附属同济医院口腔医学中心1996至2018年收治的109例... 详细信息

目的:检测Bmi-1基因在口腔白斑细胞和组织中的表达,分析其在口腔白斑恶性转化中的作用及临床意义。方法:免疫组化法检测南京医科大学附属无锡人民医院口腔科和华中科技大学同济医学院附属同济医院口腔医学中心1996至2018年收治的109例口腔白斑患者(其中男51例,女58例,年龄18~74岁)白斑组织样本中Bmi-1表达,分析其表达水平与口腔白斑患者临床病理参数、预后的关联;采用荧光定量逆转录聚合酶链反应(RT-qPCR)和Western蛋白印迹法检测正常口腔黏膜上皮细胞、口腔白斑细胞、口腔鳞癌细胞、口腔白斑组织和配对的邻近正常组织中Bmi-1基因mRNA和蛋白表达水平;在口腔白斑细胞系Leuk-1中沉默Bmi-1基因表达后分析其对Leuk-1细胞增殖、克隆形成和凋亡的影响。结果:Bmi-1在重度和轻度上皮异常增生的口腔白斑组织中的蛋白表达水平差异有统计学意义(6819±994比4713±372,P=0.017);Bmi-1高表达的口腔白斑患者的未癌变生存率为65.5%(36/55),低于Bmi-1低表达者[88.9%(48/54),P=0.003]。Cox回归分析显示Bmi-1的表达水平可作为预测口腔白斑恶性转化的预测指标(HR=2.522,95%CI:1.128~5.640,P=0.024)。Bmi-1在口腔白斑组织中的mRNA表达水平为0.455±0.120,高于其在邻近正常口腔黏膜组织(0.063±0.009,P=0.014);Bmi-1在随访期发生癌变的口腔白斑组织中的mRNA表达水平(1.405±0.397)高于未发生癌变的口腔白斑组织(0.145±0.017,P<0.001);在口腔白斑细胞系Leuk-1中转染Bmi-1-shNC和Bmi-1-shRNA2腺病毒后,二者的克隆形成数(824±40比414±38,P=0.002)和细胞凋亡率(17.7%±2.3%比36.0%±2.0%,P=0.004)差异均有统计学意义。结论:Bmi-1表达上调促进了口腔白斑细胞的恶性生物学行为,Bmi-1表达可作为预测口腔白斑恶性转化的分子标志物。

关键词：白斑,口腔 Bmi-1基因恶性转化预后预测

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乙酰肝素酶在不同转移潜能口腔鳞癌中的表达及临床意义

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中华医学杂志 2016年第38期96卷 3047-3052页

作者：吴祥冰王荣艳龚中坚於俊朱声荣南京医科大学附属无锡人民医院口腔科江苏省214023 无锡市儿童医院口腔科华中科技大学同济医学院附属同济医院口腔医学中心

目的探讨乙酰肝素酶（HPSE）在高、低转移潜能的口腔鳞癌中的表达以及临床意义。方法采用实时荧光定量逆转录PCR（RT－qPCR）和Western印迹检测高转移潜能口腔鳞癌细胞（HN12）和低转移潜能口腔鳞癌细胞（HN4）以及6对口腔鳞癌原发病灶... 详细信息

目的探讨乙酰肝素酶（HPSE）在高、低转移潜能的口腔鳞癌中的表达以及临床意义。方法采用实时荧光定量逆转录PCR（RT－qPCR）和Western印迹检测高转移潜能口腔鳞癌细胞（HN12）和低转移潜能口腔鳞癌细胞（HN4）以及6对口腔鳞癌原发病灶和转移病灶组织中HPSE的表达水平；免疫组织化学检测华中科技大学同济医学院附属同济医院口腔医学中心和南京医科大学附属无锡人民医院口腔科1999至2009年131例手术患者石蜡口腔鳞癌组织中HPSE的表达情况，分析其表达水平与口腔鳞癌患者临床病理参数、预后的相关性；细胞迁移和侵袭实验分析沉默HPSE基因表达后对HN12细胞迁移和侵袭能力的影响。结果HPSE在HN12细胞中的表达较HN4细胞上调约2倍（P〈0.000 1）；HPSE在口腔鳞癌转移病灶组织中的表达较配对的原发病灶组织上调约4.5倍（P〈0.000 1）；HPSE在口腔鳞癌中的表达水平与淋巴结转移（P〈0.000 1）和肿瘤TNM分期（P＝0.012）显著相关，HPSE低表达的口腔鳞癌患者总体生存率显著高于HPSE高表达患者（P＝0.000 3）；多因素Cox回归分析显示，HPSE表达水平与口腔鳞癌患者预后显著相关（HR＝2.203；95% CI：1.203～3.988，P＝0.009）；沉默HPSE基因表达能显著抑制HN12细胞的迁移和侵袭（P＝0.008）能力。结论HPSE表达上调参与了口腔鳞癌的侵袭和转移，HPSE可以作为预测口腔鳞癌患者预后的生物标志物。

关键词：口腔鳞状细胞癌乙酰肝素酶肿瘤转移

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乙酰肝素酶和核转录因子кB在涎腺肿瘤中的表达及其临床意义

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实用口腔医学杂志 2009年第5期25卷 698-701页

作者：吴祥冰朱声荣丁蕾谢三祥湖北省恩施土家族苗族自治州中心医院口腔科 445000 湖北武汉华中科技大学同济医学院附属同济医院口腔医学中心

目的:检测涎腺肿瘤中乙酰肝素酶(HPA)和核转录因子кB(NF-κB)的表达,探讨两者之间的关系及其临床意义。方法:采用免疫组化SP法检测42例涎腺良性肿瘤、44例涎腺恶性肿瘤中HPA和NF-κB表达。结果:HPA和NF-κB在涎腺恶性肿瘤组中阳性表达... 详细信息

目的:检测涎腺肿瘤中乙酰肝素酶(HPA)和核转录因子кB(NF-κB)的表达,探讨两者之间的关系及其临床意义。方法:采用免疫组化SP法检测42例涎腺良性肿瘤、44例涎腺恶性肿瘤中HPA和NF-κB表达。结果:HPA和NF-κB在涎腺恶性肿瘤组中阳性表达率分别为61.4%和65.9%,显著高于涎腺良性肿瘤组(P0.05)。HPA的表达与NF-κB的表达呈正相关(r=0.7602,P<0.05)。结论:NF-κB在体内可能参与了HPA的表达调控,并与HPA协同作用促进涎腺恶性肿瘤的侵袭和转移。

关键词：乙酰肝素酶核转录因子кB 涎腺肿瘤

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