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Supplementing daidzein in diets improves the reproductive performance, endocrine hormones and antioxidant capacity of multiparous sows

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Animal Nutrition 2021年第4期7卷 1052-1060页

作者： Yan Li Guoru He Daiwen Chen Bing Yu Jie Yu Ping Zheng Zhiqing Huang Yuheng Luo Junqiu Luo xiangbing Mao Hui Yan Jun He Institute of Animal Nutrition Sichuan Agricultural UniversityKey Laboratory of Animal Disease-Resistance NutritionMinistry of EducationChengdu 611130China New Hope Liuhe Co.Ltd. China

Certain hormones play important roles in modulating mammalian reproductive *** is a well-known isoflavonic phytoestrogen that possesses oestrogenic *** study was conducted to probe the effects of daidzein supplementation in gestation diets on the reproductive performance in sows.A total of 120 multiparous sows(Landrace x Yorkshire)were randomly assigned to 2 groups(n=60)and fed either a base diet(control)or one containing 200 mg/kg daidzein during *** discovered that daidzein supplementation significantly increased the total number of piglets born per litter and number of piglets born alive per litter(P<0.05),decreased the farrowing time(P<0.05)and increased the serum oestrogen and progesterone concentrations(P<0.05)at 35 d of ***,serum immunoglobulin G(IgG)concentration and superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)activities were higher in the daidzein-treated group than in the control group at35 d of gestation(P<0.05).Daidzein increased the serum SOD activity and total anti-oxidative capacity(T-AOC)at 85 d of gestation(P<0.05).Interestingly,daidzein elevated the expression levels of the sodium-coupled neutral amino acid transporter 1(SLC38 A1)and insulin-like growth factor 1(IGF-1)genes in the placenta(P<0.05).These results suggest that daidzein ingestion could improve sow reproductive performance by changing serum hormones,elevating anti-oxidative capacity and upregulating critical functional genes in the placenta.

关键词： Daidzein Immunity Anti-oxidative Reproductive performance Sow

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Magnetic resonance-guided focused ultrasound for essential tremor:a prospective,single center,single-arm study

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Neural Regeneration Research 2024年第9期19卷 2075-2080页

作者： Rui Zong Xuemei Li Chunyu Yin Jianfeng He Dekang Zhang xiangbing Bian Lichao Huang Jiayou Zhou Zhipei Ling Lin Ma Xin Lou Longsheng Pan Xinguang Yu Seniro Department of Neurosurgery The First Medical Center of the PLA General HospitalBeijingChina Clinics of Cadre Department of OutpatientThe First Medical Center of the PLA General HospitalBeijingChina Department of Radiology The First Medical Center of the PLA General HospitalBeijingChina

The safety and effectiveness of magnetic resonance-guided focused ultrasound thalamotomy has been broadly established and validated for the treatment of essential *** 2018,the first magnetic resonance-guided focused ultrasound system in Chinese mainland was installed at the First Medical Center of the PLA General *** prospective,single center,open-label,single-arm study was part of a worldwide prospective multicenter clinical trial(*** Identifier:NCT03253991)conducted to confirm the safety and efficacy of magnetic resonance-guided focused ultrasound for treating essential tremor in the local *** 2019 to 2020,10 patients with medication refractory essential tremor were recruited into this open-label,single arm *** treatment efficacy was determined using the Clinical Rating Scale for *** was evaluated according to the incidence and severity of adverse *** of the subjects underwent a unilateral thalamotomy targeting the ventral intermediate *** the baseline assessment,the estimated marginal mean of the Clinical Rating Scale for Tremor total score was 58.3±3.6,and this improved after treatment to 23.1±6.4 at a 12-month follow-up assessment.A total of 50 adverse events were recorded,and 2 were defined as *** most common intraoperative adverse events were nausea and *** most frequent postoperative adverse events were paresthesia and equilibrium *** of the adverse events were mild and usually disappeared within a few *** findings suggest that magnetic resonance-guided focused ultrasound for the treatment of essential tremor is effective,with a good safety profile,for patients in Chinese mainland.

关键词： efficacy essential tremor magnetic resonance-guided focused ultrasound(MRgFUS) movement disorder safety stereotactic therapy thalamotomy ventral intermediate nucleus

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Tannic acid prevents post-weaning diarrhea by improving intestinal barrier integrity and function in weaned piglets

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Journal of Animal Science and Biotechnology 2021年第2期12卷 700-710页

作者： Jie Yu Yanyan Song Bing Yu Jun He Ping Zheng xiangbing Mao Zhiqing Huang Yuheng Luo Junqiu Luo Hui Yan Quyuan Wang Huifen Wang Daiwen Chen Animal Nutrition Institute Sichuan Agricultural UniversityChengdu 611130People’s Republic of China Key Laboratory for Animal Disease-Resistance Nutrition Ministry of Education of ChinaChengdu 611130People’s Republic of China

Background:Tannic acid(TA)is potential to reduce diarrhea in weaning pigs,but knowledge about the influence of TA on intestinal barrier integrity and function is still *** experiment was conducted to investigate the effects of dietary TA supplementation on growth performance,diarrhea rate,intestinal barrier integrity and function of weaned ***:A total of 108 crossbred(Duroc×Landrace×Yorkshire)piglets,with an initial average body weight of 6.60±0.27 kg,were allotted to 3 groups(6 pigs/pen and 6 replicates/group)in a randomized complete block design according to their gender and body *** were fed the basal diet with 0(control,CON),0.2%and 1.0%TA,*** trial lasted for 28 ***:Compared with the CON group,dietary 0.2%and 1.0%TA supplementation didn’t affect ADFI,ADG and F:G(P>0.05),but reduced diarrhea rate,diarrhea index and diarrhea score of piglets(P<0.05),reduced diamine oxidase(DAO)activity and D-lactic acid concentration in serum(P<0.01).The higher occludin expression and localization were observed in the duodenum,jejunum and ileum after supplementation with 0.2%or 1.0%TA(P<0.05).Adding 0.2%TA to diet significantly decreased crypt depth,increased villus height/crypt depth ratio in the duodenum(P<0.05),and dietary 1.0%TA tended to decrease crypt depth(P<0.10)and significantly decreased villus height(P<0.05)of the ***,lower malondialdehyde content in the ileum was detected in the pigs fed 1.0%TA(P<0.05).In the duodenum,both 0.2%and 1.0%TA groups had higher occludin(OCLN)mRNA and 0.2%TA group had higher zonula occludens-2(ZO-2)level(P<0.05).Meanwhile,dietary 1.0%TA supplementation tended to up-regulate OCLN mRNA levels in the jejunum(P<0.10)and 0.2%TA supplementation tended to up-regulate zonula occludens-1(ZO-1)mRNA levels in the ileum(P<0.10).Conclusion:In conclusion,dietary supplementation of 0.2%or 1.0%TA could effectively alleviate postweaning diarrhea without altering growth performance in weaned piglets,wh

关键词： Intestine barrier Post-weaning diarrhea Tannic acid Weaned piglets

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The effect of Laves phase on heavy-ion radiation response of Nb-containing FeCrAl alloy for accident-tolerant fuel cladding

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Fundamental Research 2022年第3期2卷 437-446页

作者： Hang Xu Shuyao Si Yipeng Li xiangbing Liu Wenqing Li Changzhong Jiang Shijun Zhao Hui Wang Xiangheng Xiao Department of Physics and Hubei Nuclear Solid Physics Key Laboratory Wuhan UniversityWuhan 430072China Institute of Science and Technology of The 404 Company Limited China National Nuclear CorporationJiayuguan 735100China College of Energy Xiamen UniversityXiamen 361102China Suzhou Nuclear Power Research Institute Suzhou 215004China Department of Mechanical Engineering City University of Hong KongHong Kong 999077China Science and Technology Reactor Fuel and Material Laboratory Nuclear Power Institute ChinaChengdu 610041China

As a promising candidate material for the accident tolerant fuel cladding in light water reactors,the Nb-containing FeCrAl alloy has shown outstanding out-of-pile service performance due to the Laves phase *** this work,the radiation response in FeCrAl alloys with gradient Nb content under heavy ion radiation has been *** focus is on the effect of the Laves phase on irradiation-induced defects and *** found that the phase boundary between the matrix and Laves phase can play a critical role in capturing radiation defects,as verified by in-situ heavy-ion radiation experiments and molecular dynamic ***,the evolution of Laves phase under radiation is ***-induced amorphization and segregations observed at high radiation doses will deepen the fundamental understanding of the stability of Laves phases in the radiation environment.

关键词： Accident-tolerant fuel cladding FeCrAl Laves phase In-situ radiation Segregation

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Effects of benzoic acid,Bacillus coagulans and oregano oil combined supplementation on growth performance,immune status and intestinal barrier integrity of weaned piglets

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Animal Nutrition 2020年第2期6卷 152-159页

作者： Junning Pu Daiwen Chen Gang Tian Jun He Ping Zheng xiangbing Mao Jie Yu Zhiqing Huang Junqiu Luo Yuheng Luo Bing Yu Key Laboratory for Animal Disease-Resistance Nutrition Ministry of EducationInstitute of Animal NutritionSichuan Agricultural UniversityChengdu611130China

This experiment was conducted to investigate the effects of benzoic acid,Bacillus coagulans and oregano oil combined supplementation on growth performance,immune status and intestinal barrier integrity of *** a 26-d experiment,25 piglets were randomly assigned to 5 treatments:1)a basal diet,negative control(NC),2)NC added with antibiotics,positive control(PC);3)NC added with benzoic acid at 3,000 g/t and Bacillus coagulans at 400 g/t(AB);4)NC added with benzoic acid at 3,000 g/t and o regano oil at 400 g/t(AO);5)NC added with 3,000 g/t benzoic acid and Bacillus coagulans at 400 g/t and oregano oil at 400 g/t(ABO);On d 27,all piglets were euthanized to obtain jejunal mucosa to measure immune status and intestinal barrier *** showed that pigs fed AB diet increased the final body weight and average daily body weight gain and decreased the ratio of feed to gain co mpared with NC group(P<0.05).Co mpared with NC group,AB,AO and ABO decreased serum tumor necrosis factor-a concentration and ABO decreased interleukin-1βconcentration in serum and jejunal mucosa(P<0.05).Compared with NC group,AB upregulated mRNA expressions of sodium-glucose cotransportel,claudin-1,occludin and mucin2 in jejunal mucosa and the populations of Bifidobacterium and Bacillus in cecal digesta(P<0.05).Compared with NC group,ABO increased jejunal mucosal occludin mRNA abundance and Bifidobacterium population in cecal digesta,and decreased Escherichia coli population in cecal digesta(P<0.05).Furthermore,AB and ABO increased Bacillus population in cecal digesta compared with PC group(P<0.05).These results indicated that dietary AB supplementation could improve growth performance and intestinal barrier integrity of piglets when fed antibiotic-free diets,which was possibly associated with the improvement of immune status and intestinal *** ABO supplementation is also beneficial to improve immune status and intestinal barrier integrity and micro flora of piglets.

关键词： Benzoic acid Bacillus coagulans Oregano oil Immune status Intestinal barrier integrity Piglets

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Association of fluid-attenuated inversion recovery vascular hyperintensity with ischaemic events in internal carotid artery or middle cerebral artery occlusion

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Stroke & Vascular Neurology 2023年第1期8卷 69-76页

作者： Jinhao Lyu Jianxing Hu Xinrui Wang xiangbing Bian mengting Wei Liuxian Wang Qi Duan Yina Lan Dekang Zhang Xueyang Wang Tingyang Zhang Chenglin Tian Xin Lou Radiology Chinese PLA General Hospital/Chinese PLA Medical SchoolBeijingChina Radiology Chinese PLA General HospitalBeijingChina Neurology Chinese PLA General HospitalBeijingChina

Background and purpose Individuals with intracranial artery occlusion have high rates of ischaemic events and *** has been challenging to identify patients who had high-risk stroke using a simple,valid and non-invasive screening *** study aimed to investigate whether fluid-attenuated inversion recovery(FLAIR)vascular hyperintensity(FVH),a specific imaging sign on the FLAIR sequence,could be a predictor of ischaemic events in a population with internal carotid artery(ICA)or middle cerebral artery(MCA)*** We retrospectively analysed 147 patients(mean 60.43±12.83 years)with 149 lesions,including 37 asymptomatic and 112 symptomatic cases of ICA or MCA *** occlusion was considered if ischaemic events were present in the relevant territory within 90 *** Alberta Stroke Program Early Computed Tomography Score(FVH-ASPECTS:0-7,with 0 indicating absence of FVH and 7 suggesting prominent FVH)and collateral circulation grade were assessed for each *** logistic regression analysis was performed to detect independent markers associated with symptomatic *** A lower FVH-ASPECTS was associated with a more favourable collateral circulation grade(rho=−0.464,p<0.0001).The FVH-ASPECTS was significantly lower in the asymptomatic occlusion group than in the symptomatic occlusion group(p<0.0001).FVH-ASPECTS(Odd ratio,2.973;95%confidence interval,1.849 to 4.781;p<0.0001)was independently associated with symptomatic status after adjustment for age,sex,lesion location and collateral circulation grade in the multivariate logistic *** area under the curve was 0.861 for the use of FVH-ASPECTS to identify symptomatic *** The ability to discriminate symptomatic from asymptomatic occlusion suggests that FVH may be a predictor of *** a simple imaging sign,FVH may serve as a surrogate for haemodynamic impairments and can be used to identify high-risk stroke cases early in ICA or

关键词： occlusion fluid cerebral

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All-trans retinoic acid alleviates transmissible gastroenteritis virus-induced intestinal inflammation and barrier dysfunction in weaned piglets

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Journal of Animal Science and Biotechnology 2024年第3期15卷 1131-1144页

作者： Junning Pu Daiwen Chen Gang Tian Jun He Ping Zheng Zhiqing Huang xiangbing Mao Jie Yu Yuheng Luo Junqiu Luo Hui Yan Aimin Wu Bing Yu Institute of Animal Nutrition Sichuan Agricultural UniversityChengdu 611130SichuanPeople’s Republic of China Key Laboratory of Animal Disease-Resistance Nutrition Ministry of EducationMinistry of Agriculture and Rural AffairsKey Laboratory of Sichuan Province611130 ChengduSichuanPeople’s Republic of China

Background Transmissible gastroenteritis virus(TGEV)is one of the main pathogens causing severe diarrhea of *** pathogenesis of TGEV is closely related to intestinal ***-trans retinoic acid(ATRA)is the main active metabolite of vitamin A,which has immunomodulatory and anti-inflammatory ***,it is unclear whether ATRA can alleviate TGEV-induced intestinal inflammation and barrier dysfunction in *** study aimed to investigate the effects of ATRA on growth performance,diarrhea,intestinal inflammation and intesti-nal barrier integrity of TGEV-challenged *** In a 19-d study,32 weaned piglets were randomly divided into 4 treatments:Control group(basal diet),TGEV group(basal diet+TGEV challenge),TGEV+ATRA5 group(basal diet+5 mg/d ATRA+TGEV challenge)and TGEV+ATRA15 group(basal diet+15 mg/d ATRA+TGEV challenge).On d 14,piglets were orally administered TGEV or the sterile *** Feeding piglets with 5 and 15 mg/d ATRA alleviated the growth inhibition and diarrhea induced by TGEV(P<0.05).Feeding piglets with 5 and 15 mg/d ATRA also inhibited the increase of serum diamine oxidase(DAO)activ-ity and the decrease of occludin and claudin-1 protein levels in jejunal mucosa induced by TGEV,and maintained intestinal barrier integrity(P<0.05).Meanwhile,5 mg/d ATRA feeding increased the sucrase activity and the expres-sions of nutrient transporter related genes(GLUT2 and SLC7A1)in jejunal mucosa of TGEV-challenged piglets(P<0.05).Furthermore,5 mg/d ATRA feeding attenuated TGEV-induced intestinal inflammatory response by inhibit-ing the release of interleukin(IL)-1β,IL-8 and tumor necrosis factor-α(TNF-α),and promoting the secretion of IL-10 and secretory immunoglobulin A(sIgA)(P<0.05).Feeding 5 mg/d ATRA also down-regulated the expressions of Toll-like receptors and RIG-I like receptors signaling pathway related genes(TLR3,TLR4,RIG-I,MyD88,TRIF and MAVS)and the phosphorylation level of nuclear factor-κB-p65(NF-κB p65),and

关键词： All-trans retinoic acid Inflammation Intestinal barrier Piglets Transmissible gastroenteritis virus

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带斜楼盖建筑结构抗震性能研究

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建筑结构学报 2005年第6期26卷 20-26页

作者：杨想兵傅学怡周颖中建国家游泳中心设计联合体 100029

国家游泳中心“水立方”现浇钢筋混凝土斜看台楼盖跨越两个结构层,形成一个连续、复杂的空间结构。现浇斜看台楼盖建筑尺度及刚度较大,其对整体建筑结构工作性能尤其对地震作用下各类结构构件受力影响较大,不容忽视。我国《建筑抗震设... 详细信息

国家游泳中心“水立方”现浇钢筋混凝土斜看台楼盖跨越两个结构层,形成一个连续、复杂的空间结构。现浇斜看台楼盖建筑尺度及刚度较大,其对整体建筑结构工作性能尤其对地震作用下各类结构构件受力影响较大,不容忽视。我国《建筑抗震设计规范》(GB 50011—2001)对此类带现浇斜楼盖结构抗震设计尚没有明确的规定。本文结合国家游泳中心“水立方”斜看台楼盖结构设计,通过对带现浇斜看台楼盖和不带现浇斜看台楼盖两类结构在水平地震作用下的计算分析,揭示了现浇斜楼盖对整体结构工作性能及对各类结构构件地震作用下受力的影响规律,提出了此类结构的抗震设计建议。

关键词：现浇斜楼盖抗震性能

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Tannic acid extracted from gallnut prevents post-weaning diarrhea and improves intestinal health of weaned piglets

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Animal Nutrition 2021年第4期7卷 1078-1086页

作者： Yanyan Song Yong Luo Bing Yu Jun He Ping Zheng xiangbing Mao Zhiqing Huang Junqiu Luo Yuheng Luo Hui Yan Quyuan Wang Huifen Wang Daiwen Chen Jie Yu Animal Nutrition Institute Sichuan Agricultural UniversityKey Laboratory of Animal Disease-Resistance NutritionSichuan ProvinceChengdu611130China Departments of Traditional Chinese Medicine Sichuan Academy of Medical SciencesSichuan Provincial People's HospitalChengdu610031China

This experiment was conducted to evaluate the effects of different levels of tannic acid(TA)on growth performance,diarrhea rate,nutrient digestibility and intestinal health in weaned piglets.A total of 180 weaned piglets(Duroc×Landrace×Yorkshire,24 d of age,initial average BW=7.77±0.17 kg)were allotted to 5 groups(6 pigs/pen and 6 replicates/group)in a randomized complete block design according to their gender and body *** were fed a basal diet,or the basal diet supplemented with 0.05%,0.1%,0.2%or 0.4%TA for 28 *** supplementary levels of TA in the diets were obtained by adding tannalbin containing 51%TA and 40.17%*** results showed that,compared with the CON group,dietary TA did not affect ADFI,ADG or F:G,and linearly reduced(P<0.01)the diarrhea rate and diarrhea index of *** were no significant effects on apparent total tract digestibility(ATTD)in the 0.05%,0.1%and 0.2%TA groups,while negative effects(P<0.05)on apparent digestibility of crude protein and gross energy were observed in the 0.4%TA *** addition,the nutrient digestibility of dry matter,crude protein and gross energy linearly decreased(P<0.01)with the increase of TA *** of TA increased(P<0.05)the villus height of the duodenum and jejunum,as well as increased(P<0.05)catalase(CAT)activity in *** TA improved(P<0.05)the Bacillus counts in cecal ***,TA significantly improved(P<0.05)Bacillus counts and reduced(P<0.05)the Escherichia coli counts in colonic *** concentration of acetic acid,pro pionic acid,butyric acid and isovaleric acid in cecal digesta were significantly increased(P<0.05).The mRNA expression level of zonula occludens-1(Z0-1),zonula occludens-2(ZI-2),and claudin-2(CLDN-2)in the jejunum were greater(P<0.05)in TA supplemented *** study showed that,compared to the control,TA prevented post-weaning diarrhea and improved intestinal health of weaned piglets,and the appropriate level of TA supplementation woul

关键词： Tannic acid Weaned piglet Post-weaning diarrhea Intestinal health

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Gut microbiota absence and transplantation affect growth and intestinal functions:An investigation in a germ-free pig model

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Animal Nutrition 2021年第2期7卷 295-304页

作者： Hua Zhou Jing Sun Bing Yu Zuohua Liu Hong Chen Jun He xiangbing Mao Ping Zheng Jie Yu Junqiu Luo Yuheng Luo Hui Yan Liangpeng Ge Daiwen Chen Key Laboratory of Animal Disease-Resistance Nutrition ChengduSichuan 611130China Animal Nutrition Institute Sichuan Agricultural UniversityChengduSichuan 611130China Key Laboratory of Pig Industry Sciences RongchangChongqing 402460China Chongqing Academy of Animal Sciences RongchangChongqing 402460China College of Food Science Sichuan Agricultural UniversityYa'anSichuan 625014China

This study was conducted to investigate host-microbiota interactions and explore the effects of maternal gut microbiota transplantation on the growth and intestinal functions of newborns in a germ-free(GF)pig *** hysterectomy-derived GF Bama piglets were reared in 6 sterile *** them,6 were considered as the GF group,and the other 6 were orally inoculated with healthy sow fecal suspension as fecal microbiota transplanted(FMT)*** 6 piglets from natural birth were regarded as the conventional(CV)*** GF and FMT groups were hand-fed with Co60-y-irradiated sterile milk powder,while the CV group was reared by lactating Bama *** groups were fed for 21 ***,all piglets and then were switched to sterile feed for another 21 *** showed that the growth performance,nutrient digestibility,and concentrations of short-chain fatty acids in the GF group decreased(P<0.05).Meanwhile,the serum urea nitrogen concentration and digesta pH values in the GF group increased compared with those in the FMT and CV groups(P<0.05).Compared with the CV group,the GF group demonstrated upregulation in the mRNA expression levels of intestinal barrier function-related genes in the small intestine(P<0.05).In addition,the mRNA abundances of intestinal development and absorption-related genes in the small intestine and colon were higher in the GF group than in the CV and FMT groups(P<0.05).The FMT group exhibited greater growth performance,lipase activity,and nutrient digestibility(P<0.05),higher mRNA expression levels of intestinal development and barrier-related genes in the small intestine(P<0.05),and lower mRNA abundances of pro-inflammatory factor in the colon and jejunum(P<0.05)than the CV *** conclusion,the absence of gut microbes impaired the growth and nutrient digestibility,and healthy sow gut microbiota transplantation increased the growth and nutrient digestibility and improved the intestinal development and barrier function of newbor

关键词： Germ-free Host microbiota interaction Maternal gut microbiota transplantation Growth performance Intestinal function Pig model

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