Effects of benzoic acid,Bacillus coagulans and oregano oil combined supplementation on growth performance,immune status and intestinal barrier integrity of weaned piglets

作     者：Junning Pu Daiwen Chen Gang Tian Jun He Ping Zheng Xiangbing Mao Jie Yu Zhiqing Huang Junqiu Luo Yuheng Luo Bing Yu Junning Pu;Daiwen Chen;Gang Tian;Jun He;Ping Zheng;Xiangbing Mao;Jie Yu;Zhiqing Huang;Junqiu Luo;Yuheng Luo;Bing Yu

作者机构：Key Laboratory for Animal Disease-Resistance NutritionMinistry of EducationInstitute of Animal NutritionSichuan Agricultural UniversityChengdu611130China 

出 版 物：《Animal Nutrition》 (动物营养（英文版）)

年 卷 期：2020年第6卷第2期

页      面：152-159页

核心收录：

学科分类：0710[理学-生物学] 090502[农学-动物营养与饲料科学] 0905[农学-畜牧学] 0906[农学-兽医学] 09[农学] 

基　　金：supported by the National Key Research and Development Program of China(2018YFD0500605) the Science and Technology Support Program of Sichuan Province(2014NZ0043,2016NZ0006) 

主　　题：Benzoic acid Bacillus coagulans Oregano oil Immune status Intestinal barrier integrity Piglets 

摘      要：This experiment was conducted to investigate the effects of benzoic acid,Bacillus coagulans and oregano oil combined supplementation on growth performance,immune status and intestinal barrier integrity of *** a 26-d experiment,25 piglets were randomly assigned to 5 treatments:1)a basal diet,negative control(NC),2)NC added with antibiotics,positive control(PC);3)NC added with benzoic acid at 3,000 g/t and Bacillus coagulans at 400 g/t(AB);4)NC added with benzoic acid at 3,000 g/t and o regano oil at 400 g/t(AO);5)NC added with 3,000 g/t benzoic acid and Bacillus coagulans at 400 g/t and oregano oil at 400 g/t(ABO);On d 27,all piglets were euthanized to obtain jejunal mucosa to measure immune status and intestinal barrier *** showed that pigs fed AB diet increased the final body weight and average daily body weight gain and decreased the ratio of feed to gain co mpared with NC group(P0.05).Co mpared with NC group,AB,AO and ABO decreased serum tumor necrosis factor-a concentration and ABO decreased interleukin-1βconcentration in serum and jejunal mucosa(P0.05).Compared with NC group,AB upregulated mRNA expressions of sodium-glucose cotransportel,claudin-1,occludin and mucin2 in jejunal mucosa and the populations of Bifidobacterium and Bacillus in cecal digesta(P0.05).Compared with NC group,ABO increased jejunal mucosal occludin mRNA abundance and Bifidobacterium population in cecal digesta,and decreased Escherichia coli population in cecal digesta(P0.05).Furthermore,AB and ABO increased Bacillus population in cecal digesta compared with PC group(P0.05).These results indicated that dietary AB supplementation could improve growth performance and intestinal barrier integrity of piglets when fed antibiotic-free diets,which was possibly associated with the improvement of immune status and intestinal *** ABO supplementation is also beneficial to improve immune status and intestinal barrier integrity and micro flora of piglets.