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Real-time monitoring of DNAzyme cleavage process using fluorescent assay

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Chinese Chemical Letters 2009年第8期20卷 990-994页

作者： xiang xian meng Xiao Hai Yang Ke Min Wang Wei Hong Tan Qiu Ping Guo State Key Laboratory of Chemo/Biosensing and Chemometrics Engineering Center for BiomedicineKey Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan ProvinceCollege of Chemistry and Chemical Engineering Hunan University Changsha 410082 China

Detection of deoxyribozyme （DNAzyme） cleavage process usually needs complex and time-consuming radial labeling, gel electrophoresis and autoradiography. This paper reported an approach to detect DNAzyme cleavage process in real time using a fluorescence probe. The probe was employed as DNAzyme substrate to convert directly the cleavage information into fluorescence signal in real time. Compared with traditional approach, this non-isotope method not only brought a convenient means to monitor the DNAzyme cleavage reaction, but also offered abundant dynamic data for choosing potential gene therapeutic agents. It provides a new tool for DNAzyme research, as well as a new insight into research on human disease diagnosis. Based on this method, 8- 17deoxyribozyme （8-17DNAzyme） against hepatitis C virus RNA （HCV-RNA） was designed and the cleavage process was studied in real time. ？2009 Ke Min Wang. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All fights reserved.

关键词： DNAzyme Cleavage Real time HCV-RNA

DNA aptamer selected for specific recognition of prostate cancer cells and clinical tissues

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Chinese Chemical Letters 2017年第6期28卷 1252-1257页

作者： Zhi-xiang Huang Qin Xie Qiu-Ping Guo Ke-Min Wang xiang-xian meng Bao-Yin Yuan Jun Wan Yuan-Yuan Chen State Key Laboratory of Chemo/Biosensing and Chemometrics College of Biology College of Chemistry and Chemical Engineering Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province Hunan University Changsha 410082 China

Prostate cancer is the most common malignancy in men lack of efficient early diagnosis and therapeutics,calling for effective molecular ***,we performed cell-based systematic evolution of ligands by exponential enrichment（cell-SELEX） to obtain specific recognition of human prostate cancer cells *** aptamers were successfully obtained that can bind to target cells with high affinity and specificity.A 51-nt truncated sequence named Xq-2-C1 was identified after further elaborative analysis on the secondary *** importantly,the achieved aptamer Xq-2-C1 not only demonstrated excellent specific to target cells,but also revealed specific recognition to clinical prostate cancer *** tissue imaging results showed that Xq-2-C1 had better recognition ratio for clinical prostate cancer tissue samples（85%） compared to the random sequence（9%）.These results demonstrate that these newly generated aptamers would furnish potential applications in the early diagnosis and clinical treatment of prostate cancer.

关键词： Aptamer Cell-SELEX Prostate cancer PC-3M Clinical tissues

Ligase-based ultrasensitive detection of DNAzyme cleavage product using molecular beacon

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Chinese Chemical Letters 2012年第10期23卷 1177-1180页

作者： Hong Peng Xie xiang xian meng Hongyan Su Qing Yun Cai Yong Jun Tan Xiao Qin Huang School of Biology State Key Laboratory of Chemo/Biosensing and ChemometricsCollege of Chemistry and Chemical EngineeringHunan UniversityChangsha 410082China

Detection for deoxyribozyme （DNAzyme） cleavage usually needs complex and time-consuming radial labeling, gel electro- phoresis and autoradiography. A new approach was reported for detection DNAzyme cleavage product based on molecular beacon （MB）. Part of the loop of MB was designed to complementary to DNAzyme cleavage product. MB was employed to monitor ligation process of RNA/DNA complex and to convert directly cleavage product information into fluorescence signal. Detection limit of the assay is 0.02 nmol/L. The cleavage product of 8-17 DNAzyme against HCV-RNA was detected perfectly based on this assay. The method is fast, simple and ultrasensitive, which might hold great promise in DNAzyme reaction and DNAzyme gene therapy.

关键词： Molecular beacon Ligation DNAzyme cleavage HCV-RNA

Sensitive and enzyme-free detection for single nucleotide polymorphism using microbead-assisted toehold-mediated strand displacement reaction

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Chinese Chemical Letters 2015年第8期26卷 1031-1035页

作者： Jia-Bao Long Ying-Xin Liu Qing-Feng Cao Qiu-Ping Guo Shu-Ya Yan xiang-xian meng College of Biology State Key Laboratory of Chemo/Biosensing and Chemometrics Hunan University Department of Bioengineering and Environment Science Changsha University

This report described a free-enzyme, convenient and inexpensive genotyping biosensor capable of detecting single nucleotide polymorphism at normal temperature based on the combination of toeholdmediated strand displacement reaction（toehold-SDR） and microbead-capture technique. The biosensor consists of a pre-hybridized strand formed by a reporter probe and a capture probe. In the presence of a mutant sequence, there is no toehold-mediated strand displacement and the reporter probe cannot be released from the pre-hybridized strand. Microbeads capture the fluorescent pre-hybridized strand through biotin–streptavidin interaction, so microbeads give out significant fluorescence signal, while there is no fluorescence in the solution. However, in the presence of a matched target, the strand displacement is effectively initiated and the reporter probe is released from pre-hybridized strand. After adding microbeads, the solution produces bright fluorescence, while microbeads have no obvious *** are identified conveniently according to the fluorescence intensity of the solution. The method provides a simple and inexpensive strategy to detect point mutation. Moreover, this biosensor shows the linear relationship in the range of 1–40 nmol/L and reaches a detection limit of 0.3 nmol/L.？ 2015 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical *** by Elsevier B.V. All rights reserved.

关键词： strand capture biosensor convenient detecting capable inexpensive reporter bright matched

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PdTe2纳米薄膜的电和热输运特性研究

工程热物理学报 2020年第12期41卷 3041-3045页

作者：缪婷婷向梦贤李大炜中国石油大学(北京)机械与储运工程学院过程流体过滤与分离技术北京市重点实验室北京102249

作为第二类狄拉克半金属,PdTe2由于具有低温超导电性和特殊的能带结构,研究者们对该材料的探索集中在超导和拓扑特性,对室温下PdTe2纳米薄膜的电和热输运性质的研究尚未报道。本文采用直流通电加热法测量了厚度为136 nm的PdTe2薄膜在30... 详细信息

作为第二类狄拉克半金属,PdTe2由于具有低温超导电性和特殊的能带结构,研究者们对该材料的探索集中在超导和拓扑特性,对室温下PdTe2纳米薄膜的电和热输运性质的研究尚未报道。本文采用直流通电加热法测量了厚度为136 nm的PdTe2薄膜在300 K下的电导率、热导率和磁致电阻。薄膜在室温下的电导率为3.7×10^6 S.m^-1,在迄今已研究的二维材料中是非常高的,甚至与一些传统金属铁、锌、锡和铂相当。室温下的热导率为130 W.m^-1.K^-1,远高于黄铜、青铜和铅等传统金属。薄膜的磁致电阻随磁场强度增大而增大,且正比于磁场强度的二次方,14 T时最大值为0.94%。

关键词： PdTe2纳米薄膜电导率热导率磁致电阻

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论高校学习型党组织建设路径

广西师范大学学报（哲学社会科学版） 2011年第5期47卷 143-146页

作者：向先孟广西师范大学体育学院广西桂林541004

高校学习型党组织建设存在着党组织成员学习动机不强,学习内容缺乏更新,学习形式老套陈旧,学习管理机制不稳等问题。高校应在了解个人学习与团体学习相结合,理论学习与实际教学和科研工作相结合,文化建设与营造浓厚学习氛围相结合,理论... 详细信息

高校学习型党组织建设存在着党组织成员学习动机不强,学习内容缺乏更新,学习形式老套陈旧,学习管理机制不稳等问题。高校应在了解个人学习与团体学习相结合,理论学习与实际教学和科研工作相结合,文化建设与营造浓厚学习氛围相结合,理论研究和理论学习创新相结合等学习型党组织基本要求的基础上,明确学习目的、合理安排学习内容、创新学习方法、完善学习机制。

关键词：高校学习型党组织问题建设

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由武术引发的体育社会功能问题的探讨

唐山师范学院学报 2010年第2期32卷 89-91页

作者：姜国安张旭昌向先孟广西师范大学体育学院广西桂林541004

对武术在当今社会的价值进行分析,以促使人们更好地认识武术、了解武术,进而喜爱武术,并且愿意为武术的发展贡献自己的一份力量,把我们的民族传统文化精髓更好地传扬出去。

关键词：武术社会功能价值

New Algebraic Structures from Hermitian One-Matrix Model

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Acta Mathematica Sinica,English Series 2017年第9期33卷 1193-1205页

作者： xiang Mao DING Yu Ping LI Ling xian meng Institute of Applied Mathematics Academy of Mathematics and Systems ScienceChinese Academy of SciencesBeijing 100190P.R.China College of Mathematics and Information Science Zhengzhou University of Light IndustryZhengzhou 450002P.R.China

Virasoro constraint is the operator algebra version of one-loop equation for a Hermitian one-matrix model, and it plays an important role in solving the model. We construct the realization of the Virasoro constraint from the Conformal Field Theory （CFT） method. From multi-loop equations of the one-matrix model, we get a more general constraint. It can be expressed in terms of the operator algebras, which is the Virasoro subalgebra with extra parameters. In this sense, we named as generalized Virasoro constraint. We enlarge this algebra with central extension, this is a new kind of algebra, and the usual Virasoro algebra is its subalgebra. And we give a bosonic realization of its subalgebra.

关键词： Matrix model Virasoro constraint conformal symmetry

Synthesis of nano-TiO2 assisted by diethylene glycol for use in high efficiency dye-sensitized solar cells

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Chinese Chemical Letters 2017年第4期28卷 765-770页

作者： Lin Liu xiang-Mei Yu Bao Zhang Shu-xian meng Ya-Qing Feng School of Chemical Engineer and Technology Tianjin University Tianjin 300072 China Collaborative Innovation Center of Chemical Science and Engineering Tianjin 300072 China

The performance of dye-sensitized solar cells（DSSCs） consisting of anatase TiO_2 nanoparticles that were synthesized via a hydrothermal method was *** synthesized TiO_2 nanoparticles were characterized by X-ray diffraction（XRD）,nitrogen sorption analysis,scanning electron microscopy（SEM）,high resolution transmission electron microscopy（HRTEM）,and UV-vis *** the J-Vcurve,electrochemical impedance spectroscopy（EIS）,and open-circuit voltage decay（OCVD） measurement were applied to evaluate the photovoltaic performance of *** with the commercial TiO_2nanoparticles（P25）,the synthesized-TiO_2 nanoparticles showed better *** adding diethylene glycol（DEG） before the hydrothermal process,the synthesized TiO_2 nanoparticles（hereafter referred to as TiO_2-DEG particles） shows narrower size distribution,larger specific surface area,higher crystallinity,and less surface defects than TiO_2（DEG free） *** analysis of photovoltaic properties of DSSCs based on TiO_2-DEG particles showed that the recombination of electron-hole pairs was decreased and the trapping of carries in grain boundaries *** was believed that the photoelectrode fabricated with the as-prepared TiO_2 nanoparticles improved the loading amount of dye sensitizers（N719）.and enhanced the photocurrent of the *** a result,the TiO_2-DEG particle based cells achieved a photo-to-electricity conversion efficiency（η） of 7.90%,which is higher than 7.53%for the cell based on TiO2（DEG free） and 6.59%for the one fabricated with P25.

关键词： Hydrothermal TiO2 Diethylene glycol Photoelectrode Dye-sensitized solar cells