Lineal DNA logic gate for microRNA diagnostics with strand displacement and fluorescence resonance energy transfer

作     者：Tian-Tian Meng Ying-Xin Liu Meng-Tan Liu Jiao-Bao Long Qing-Feng Cao Shu-Ya Yan Xiang-Xian Meng 

作者机构：College of BiologyThe High School Attach to Hunan University Hunan University School of Life ScienceXiamen University 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2015年第26卷第9期

页      面：1179-1182页

核心收录：

学科分类：100208[医学-临床检验诊断学] 1002[医学-临床医学] 10[医学] 

基　　金：supported by National Natural Science Foundation of China(No.21275043) National Basic Research Program of China under Grants(No.2009CB421601) 

主　　题：Molecular logic gate Strand displacement Fluorescence resonance energy transfer 

摘      要：Designing molecular logic gates to operate programmably for molecular diagnostics in molecular computing still remains ***,we designed a novel linear DNA logic gates for microRNA analysis based on strand displacement and fluorescence resonance energy transfer（FRET）.Two labeled strands closed each other produce to FRET through hybridization with a complementary strand to form a basic work unit of logic *** indicators of heart failure（microRNA-195 and microRNA-21） were selected as the logic inputs and the fluorescence mode was used as the logic *** have demonstrated that the molecular logic gate mechanism worked well with the construction of YES and AND gates.