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Pterygium epithelium abnormal differentiation related to activation of extracellular signal-regulated kinase signaling pathway in vitro

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International Journal of Ophthalmology(English edition) 2015年第6期8卷 1118-1125页

作者： Juan Peng xiang-yin sha Yi Liu Rui-Ming Yang Ye Wen Department of Ophthalmology the Second Affiliated Hospital of Guangzhou Medical University

AIMTo investigate whether the abnormal differentiation of the pterygium epithelium is related to the extracellular signal-regulated kinase (ERK) signaling pathway in *** expression levels of phosphorylated ERK (P-ERK), keratin family members including K19 and K10 and the ocular master control gene Pax-6 were measured in 16 surgically excised pterygium tissues and 12 eye bank conjunctiva. In colony-forming cell assays, the differences in clone morphology and in K10, K19, P-ERK and Pax-6 expression between the head and body were investigated. When cocultured with the ERK signaling pathway inhibitor PD98059, the changes in clone morphology, colony-forming efficiency, differentiated marker K10, K19 and Pax-6 expression and P-ERK protein expression level were examined by immunoreactivity and Western blot *** expression of K19 and Pax-6 decreased in the pterygium, especially in the head. No staining of K10 was found in the normal conjunctiva epithelium, but it was found to be expressed in the superficial cells in the head of the pterygium. Characteristic upregulation of P-ERK was observed by immunohistochemistry. The clone from the head with more differentiated cells in the center expressed more K10, and the clone from the body expressed more K19. The P-ERK protein level increased in the pterygium epithelium compared with conjunctiva and decreased when cocultured with PD98059. The same medium with the ERK inhibitor PD98059 was more effective in promoting clonal growth than conventional medium with 3T3 murine feeder layers. It was observed that the epithelium clone co-cultured with the inhibitor had decreased K10 expression and increased K19 and Pax-6 *** suggest ERK signaling pathway activation might play a role in the pterygium epithelium abnormal differentiation.

关键词： abnormal differentiation epithelial cells pterygium extracellular signal-regulated kinase signaling pathway in vitro

Avastin和地塞米松在大鼠角膜新生血管中的作用

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眼科研究 2010年第7期28卷 591-595页

作者：沙翔垠宋莉广州医学院第二附属医院眼科 510260

目的探讨眼表应用avastin、地塞米松及联合用药对SD大鼠角膜新生血管(CNV)的作用及机制。方法建立碱烧伤诱导大鼠CNV模型,分为4组:地塞米松组给予质量分数0.1%地塞米松局部点眼;avastin组给予5.0g/Lavastin;联合组给予5.0g/Lavastin+0.1... 详细信息

目的探讨眼表应用avastin、地塞米松及联合用药对SD大鼠角膜新生血管(CNV)的作用及机制。方法建立碱烧伤诱导大鼠CNV模型,分为4组:地塞米松组给予质量分数0.1%地塞米松局部点眼;avastin组给予5.0g/Lavastin;联合组给予5.0g/Lavastin+0.1%地塞米松;对照组给予生理盐水。采用裂隙灯观察、免疫组织化学染色等方法观察各组CNV的面积和血管内皮生长因子(VEGF)的表达。结果第6天时,对照组CNV生长接近高峰,血管粗大、密集,交织成网状;地塞米松组和avastin组CNV较短,血管稀疏、细长;联合组CNV较短,血管较稀疏、细小。实验后3、6、12d,3个组CNV面积比较差异均有统计学意义(F=145.659,P=0.000;F=296.370,P=0.000;F=148.008,P=0.000),组间多重比较显示avastin组、地塞米松组及联合组CNV面积均小于对照组,差异均有统计学意义(P0.05)。第7天时,组织病理学检测显示对照组角膜可见大量炎性细胞浸润,基质层大量CNV,胶原纤维排列紊乱;地塞米松组、avastin组角膜基质排列趋于整齐,CNV明显减少,腔小且分布稀疏,未见大量炎性细胞浸润;联合组基质层胶原纤维排列规则,CNV显著减少,炎性细胞浸润较少。免疫组织化学检测提示对照组角膜全层VEGF表达明显增强,其表达部位主要分布在CNV区域和上皮全层。地塞米松组和avastin组CNV密度减低,角膜基质层CNV区域的VEGF表达减少;联合组CNV密度明显减低,VEGF表达微弱。结论 Avastin和地塞米松均能抑制CNV,二者联合用药具有协同作用,可降低CNV和炎性细胞浸润。

关键词：角膜新生血管 avastin 地塞米松血管内皮生长因子

翼状胬肉上皮细胞表达的研究中β-catenin和Cyclin D1表达的研究

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中国实用眼科杂志 2012年第8期30卷 962-965页

作者：彭娟沙翔垠广州医学院第二附属医院眼科广东广州510260

目的探讨翼状胬肉上皮细胞的病理改变和β-catenin／Wnt信号通路及其下游靶基因cyclinD1的表达。并比较其与复发性翼状胬肉的表达差异。方法收集翼状胬肉组织26例，其中复发翼状胬肉8例。正常结膜组织6例。冰冻包埋后行HE和免疫组织化... 详细信息

目的探讨翼状胬肉上皮细胞的病理改变和β-catenin／Wnt信号通路及其下游靶基因cyclinD1的表达。并比较其与复发性翼状胬肉的表达差异。方法收集翼状胬肉组织26例，其中复发翼状胬肉8例。正常结膜组织6例。冰冻包埋后行HE和免疫组织化学和免疫荧光染色，检测上皮细胞中β-catenin和CyclinD1的表达。并作相关分析。结果翼状胬肉上皮细胞增生，上皮细胞中β-catenin和CyclinD1表达水平明显高于正常结膜。β-catenin表达平均阳性细胞数124．85±9．25，积分光密度826．56±10．25。CyclinD1表达平均阳性细胞数90．40±9．63，积分光密度531．85±12．36。β-catenin和Cv—clinD1表达水平具有正相关。在复发性翼状胬肉上皮细胞中β-catenin和CyclinD1表达水平较初发翼状胬肉有增加。其中β-catenin表达平均阳性细胞数295．12±11．26，积分光密度1145．25±16．58。Cyclin D1表达平均阳性细胞数162．54±9．24，积分光密度856．26±13．85，差异有统计学意义。结论Wnt／β-catenin通路可能通过CvclinD1参与翼状胬肉上皮细胞的发病过程。

关键词：翼状胬肉上皮细胞 β-catenin CyclinD1

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Bevacizumab在眼表疾病中的应用

眼科新进展 2010年第5期30卷 497-500页

作者：宋莉沙翔垠广州医学院第二附属医院眼科广东省广州市510260

眼表疾病是临床常见的致盲眼病,特别是角膜新生血管性眼病因其发病机制非常复杂,治疗棘手而成为眼科新研究领域一大难点和热点。Bevacizumab给药靶向阻断血管内皮生长因子及其受体,有效抑制新生血管生长,其抗淋巴血管性、抗纤维性有望... 详细信息

眼表疾病是临床常见的致盲眼病,特别是角膜新生血管性眼病因其发病机制非常复杂,治疗棘手而成为眼科新研究领域一大难点和热点。Bevacizumab给药靶向阻断血管内皮生长因子及其受体,有效抑制新生血管生长,其抗淋巴血管性、抗纤维性有望成为角膜移植术后免疫排斥反应、LASIK术前常规及预防用药,现综述如下。

关键词： Bevacizumab 眼表疾病血管内皮生长因子

Investigation of bubbles escape behavior from low basicity mold flux for high-Mn high-Al steels using 3D X-ray microscope

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 102-110页

作者： Qiang Liu xiang Li Shen Du Ming Gao Yanbin yin Jiongming Zhang State Key Laboratory of Advanced Metallurgy University of Science and Technology BeijingBeijing 100083China

During the continuous casting process of high-Mn high-Al steels,various types of gases such as Ar need to escape through the top of the *** which,the behavior of bubbles traversing the liquid slag serves as a restrictive link,closely associated with viscosity and the thickness of liquid *** contrast to two-dimensional surface observation,three-dimensional(3D)analysis method can offer a more intuitive,accurate,and comprehensive ***,this study employs a 3D X-ray microscope(3D-XRM)to obtained spatial distribution and 3D morphological characteristics of residual bubbles in mold flux under different basicity of liquid slag,different temperatures,and different holding *** results indicate that as basicity of slag increases from 0.52 to 1.03,temperature increases from 1423 to 1573 K,the viscosity of slag decreases,the floating rate of bubbles *** addition,when holding time increases from 10 to 30 s,the bubbles floating distance increases,and the volume fraction and average equivalent sphere diameter of the bubbles solidified in the mold flux gradually *** one word,increasing the basicity,temperature,and holding time leading to an increase in the removal rate of bubbles especially for the *** findings of bubbles escape behavior provide valuable insights into optimizing low basicity mold flux for high-Mn high-Al steels.

关键词： mold flux low basicity bubbles three-dimensional X-ray microscope viscosity

单眼双侧翼状胬肉临床特征和病理组织变化的研究

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国际眼科杂志 2017年第9期17卷 1746-1749页

作者：彭娟毛雁沙翔垠广州医科大学附属第二医院眼科中国广东省广州市510260

目的:探讨单眼双侧翼状胬肉与单眼鼻侧翼状胬肉临床特征及病理组织结构的差异。方法:回顾性研究。选取单眼双侧翼状胬肉及单眼鼻侧翼状胬肉各11例11眼患者纳入研究,观察其生长时间、组织面积大小、血管分级、透明度及泪膜破裂时间(BUT)... 详细信息

目的:探讨单眼双侧翼状胬肉与单眼鼻侧翼状胬肉临床特征及病理组织结构的差异。方法:回顾性研究。选取单眼双侧翼状胬肉及单眼鼻侧翼状胬肉各11例11眼患者纳入研究,观察其生长时间、组织面积大小、血管分级、透明度及泪膜破裂时间(BUT)。并对6例正常结膜组织及11例单眼双侧翼状胬肉组织标本采用40g/L多聚甲醛固定处理,予石蜡包埋切片后行HE染色,在光镜下观察其病理组织差异。结果:单眼双侧翼状胬肉泪液BUT较单眼鼻侧翼状胬肉显著缩短,差异有统计学意义(t=3.410,P=0.003)。单眼双侧翼状胬肉的BUT与其生长时间(r=-0.661,P<0.05)、组织大小(r=-0.775,P<0.01)、透明度(r=-0.671,P<0.05)均呈显著负相关;单眼鼻侧翼状胬肉的BUT与其组织大小(r=-0.927,P<0.01)、透明度(r=-0.764,P<0.01)均呈显著负相关。单眼双侧翼状胬肉中鼻侧上皮细胞层数较颞侧增多(t=-7.351,P<0.05)、血管数量增加(t=-7.400,P<0.05)、炎症细胞数量增多(t=-7.481,P<0.05),差异有统计学意义。结论:单眼双侧翼状胬肉较单眼鼻侧翼状胬肉泪膜稳定性差,且单眼双侧翼状胬肉中鼻侧较颞侧鳞状上皮增生明显,血管化及炎症反应显著。

关键词：单眼双侧翼状胬肉单眼鼻侧胬肉临床特征病理组织

Decentralized fault diagnosis of discrete-event systems with unreliable sensors using linear temporal logic

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Science China(Information Sciences) 2025年第1期68卷 322-341页

作者： Weijie DONG shaoyuan LI xiang yin Key Laboratory of System Control and Information Processing Ministry of EducationDepartment of Automation Shanghai Jiao Tong University

In this paper,we investigate a decentralized diagnosis problem of a discrete-evnt system(DES) subject to unreliable sensors,where the sensor observations of local diagnosers may be non-deterministic as a result of possible *** studies on decentralized robust diagnosis can only deal with different types of sensor failures separately,e.g.,all sensors suffer from the same type of sensor failures such as intermittent sensor failures or permanent sensor ***,since sensors of different local diagnosers may face different external environments and have different internal characteristics,sensors corresponding to different local diagnosers may also have their own fault *** this paper,we propose a flexible framework of decentralized diagnosis for DES subject to unreliable sensors such that sensors of different local diagnosers are permitted to have different types of sensor *** this end,we extend the existing decentralized diagnosis framework to the case where there exist common sensors broadcasting their observations to all local *** apply linear temporal logic(LTL) to constrain infinite behaviors of private sensors of local diagnosers and common ***,a new notion of φ-codiagnosability is proposed as the necessary and sufficient condition for the existence of a decentralized diagnoser that works correctly under sensors,satisfying LTL-based sensor ***,we provide an effective approach to verify the φ-codiagnosability.

关键词： discrete event systems decentralized diagnosis codiagnosability automata

玻璃体切割联合内界膜剥离术治疗黄斑裂孔性视网膜脱离

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国际眼科杂志 2012年第5期12卷 896-898页

作者：彭娟沙翔垠杨瑞明广州医学院第二附属医院眼科中国广东省广州市510260

目的:探讨玻璃体切割联合内界膜剥离术治疗黄斑裂孔性视网膜脱离的疗效和并发症。方法:收集我院2009-09/2011-12黄斑裂孔性视网膜脱离住院患者22例22眼。均采用玻璃体切割联合内界膜剥离手术方式。随访观察术后1,2,3d;1wk;1mo的患者最... 详细信息

目的:探讨玻璃体切割联合内界膜剥离术治疗黄斑裂孔性视网膜脱离的疗效和并发症。方法:收集我院2009-09/2011-12黄斑裂孔性视网膜脱离住院患者22例22眼。均采用玻璃体切割联合内界膜剥离手术方式。随访观察术后1,2,3d;1wk;1mo的患者最佳矫正视力、眼压。并对患者年龄和患病病程与术后1mo最佳矫正视力进行统计学分析。结果:行气体填充13例,行硅油填充9例。患者术后黄斑裂孔封闭率100%,视网膜均解剖复位。术后最佳矫正视力随术后恢复时间逐渐提高。眼压在术后1,2,3d有升高,7d后下降逐步恢复正常。患病病程6mo的患者术后视力恢复好。黄斑裂孔患者的术后1mo最佳矫正视力与患者年龄无相关性。结论:玻璃体切割联合内界膜剥离术是治疗黄斑裂孔视网膜脱离的有效手术方式,可提高术后视力。

关键词：玻璃体手术视网膜内界膜黄斑裂孔视网膜脱离

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盐酸万古霉素综合方案治疗眼内炎

国际眼科杂志 2012年第7期12卷 1308-1310页

作者：彭娟沙翔垠杨瑞明广州医学院第二附属医院眼科中国广东省广州市510260

目的:探讨盐酸万古霉素综合方案治疗眼内炎的临床疗效和并发症。方法:收集我院2009-07/2011-08眼内炎住院患者27例27眼,采用盐酸万古霉素综合治疗方案进行治疗。所有患者取玻璃体做细菌培养和药敏试验。采用玻璃体腔内注射盐酸万古霉素... 详细信息

目的:探讨盐酸万古霉素综合方案治疗眼内炎的临床疗效和并发症。方法:收集我院2009-07/2011-08眼内炎住院患者27例27眼,采用盐酸万古霉素综合治疗方案进行治疗。所有患者取玻璃体做细菌培养和药敏试验。采用玻璃体腔内注射盐酸万古霉素,全身使用盐酸万古霉素联合左旋氧氟沙星。炎症无明显好转者,2～4d后行玻璃体切割术,术中使用含盐酸万古霉素的灌注液。术后观察视力、眼压、前房和玻璃体混浊情况。对术后最佳矫正视力和眼压进行统计学分析。结果:患者27例眼内炎均治愈,视力均有提高。细菌培养检出率56%。革兰氏阳性菌占67%,对盐酸万古霉素敏感。革兰氏阴性菌对氧氟沙星较敏感。8例患者经过静脉滴注及玻璃体腔内注药后眼内炎症控制,19例行玻璃体切割手术。术后最佳矫正视力提高。临床症状体征好转。结论:早期细菌培养对眼内炎的治疗有重要意义。盐酸万古霉素综合治疗方案能有效治疗眼内炎。

关键词：眼内炎盐酸万古霉素玻璃体腔注射玻璃体手术