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中国神经科学学会第十届全国学术会议

作者： Yu-Feng SHAO Peng ZHAO Chao-Yu DONG Jing LI xiang-pan kong Can WANG Jun-Fan XIE Hai-Liang WANG Li-Rong DAI Yi-Ping HOU Department of Neuroscience AnatomyHistology and EmbryologyKey Laboratory of Preclinical Study for New Drugs of Gansu ProvinceSchool of Basic Medical SciencesLanzhou University

Objective Neuropeptide S(NPS) is a newly identified neuromodulator located in the brainstem and regulates various biological functions by selectively activating the NPS receptors(NPSR).High level expression of NPSR mRNA in the olfactory cortex suggests that NPS-NPSR system might be involved in the regulation of olfactory *** present study was undertaken to investigate the effects of intracerebroventricular(i.c.v.) injection of NPS or co-injection of NPSR antagonist on the olfactory behaviors,food intake,and c-Fos expression in olfactory cortex in *** Olfactory abilities in mice were gauged using the buried food test(to assess the ability of detecting volatile odors) and olfactory habituation/dishabituation test(to assess the ability of detecting and distinguishing the same and different odors).Food intake test was used to clarify the relationship between olfaction and ingestion in fasted32 h mice after *** of *** further identify potential neuronal targets of NPS in the olfactory cortex,NPS-induced Fos immunereactive(-ir) neurons were analyzed using ex vivo immunohistochemistry,and the presence of NPSR in these neurons was examined using dual-immunofluorescence ***(1) In comparison with vehicle-treated mice,*** of 0.1,0.5,and 1 nmol of NPS significantly reduced the latency to find the buried food from 73.43 ± 11.77 s to 35.74 ± 5.37 s(P < 0.001),12.72 ± 1.34 s(P < 0.001) and 24.61 ± 5.04 s(P < 0.001),*** the three doses,0.5 nmol NPS reduced the latency most(P < 0.001 and P < 0.05 compared with vehicle and 0.1 nmol NPS,respectively).In fact,high dose(1 nmol) of NPS insignificantly reduced the latency as compared to 0.1 nmol NPS(35.74± 5.37 s vs.24.61 ± 5.04 s,P = 0.24).(2) Central administration of vehicle induced a habituation to water(P < 0.05) and vanilla(P < 0.001),and a dishabituation to vanilla(P < 0.001).Mice administered NPS at 0.1 nmol were able to distinguish almond and vanilla

关键词： neuropeptide S neuropeptide S receptor olfactory function olfactory cortex [D-Val~5]NPS c-Fos

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Neuropeptide S reduced propofol- and ketamine-induced slow wave sleep through activation of its receptor in rats

Neuropeptide S reduced propofol- and ketamine-induced slow w...

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中国神经科学学会第十届全国学术会议

作者： xiang-pan kong Peng ZHAO Can WANG Jun-Fan XIE Yu-Feng SHAO Li-Rong DAI Jin LI Chao-Yu DONG Hai-Liang WANG Yi-Ping HOU Departments of Neuroscience AnatomyHistologyand EmbryologyKey Laboratory of Preclinical Study for New Drugs of Gansu ProvinceSchool of Basic Medical SciencesLanzhou University

Objective General anesthesia and natural sleep involve many obvious *** stimulatory neuropeptides such as orexin and neuropeptide S were reported to reduce anesthesia time,but relating EEG profile changes were *** present study aims to evaluate the effects of neuropeptide S and its cognate receptor(NPS-NPSR) system on sleep-waking profiles and cortical EEG power spectra in anesthetic-treated *** NPS with or without NPSR antagonist were administered intracerebroventricularly,then GABA and NMDA receptor types anesthetics propofol(50 mg/kg) and ketamine(100 mg/kg) were injected *** change of sleep-wake profiles was assessed by sleep recordings and cortical EEG power spectra were analyzed by fast Fourier *** NPS(1-2 nmol) significantly reduced the amount of propofol-induced slow wave sleep(SWS) and EEG delta ***(1-5 nmol) reduced the duration of ketamine-induced SWS characterized by EEG delta activities and significantly increased EEG theta *** inhibitory effect of NPS on anesthetics-induced SWS was through reduction of the duration of SWS episodes,but not the total number of SWS episodes and the prolonged latency of sleep.[D-Val]NPS dosing at 20 nmol significantly antagonized the arousal promoting effect of 1 nmol NPS,but injected alone did not prolong *** NPS significantly reduced anesmetics-induced SWS and EEG slow activities through selective stimulation of the NPSR and subsequent arousal pathways.

关键词： neuropetide S neuropetide S receptors anesthetics slow wave sleep rats

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Neuropeptide S Counteracts Paradoxical Sleep Deprivation-Induced Anxiety-Like Behavior and Sleep Disturbances

Neuropeptide S Counteracts Paradoxical Sleep Deprivation-Ind...

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中国睡眠研究会第十届全国学术年会

作者： Jun-Fan Xie Yu-Feng Shao Hai-Liang Wang Can Wang Guang-Fu Cui xiang-pan kong Lin-Xin Wang Yu-Nong Chen Chao-Yu Cong Hai-Lin Chen Yi-Ping Hou Departments of Neuroscience AnatomyHistologyand EmbryologyKey Laboratory of Preclinical Study for New Drugs of Gansu ProvinceSchool of Basic Medical SciencesLanzhou University Department of Human Anatomy School of MedicineHunan Normal University

Disturbed sleep is a common subjective complaint among individuals with anxiety *** deprivation increases general and specific anxiety symptoms among healthy *** amygdala is critical for regulating anxiety and also involved in mediating the effects of emotions on *** S（NPS） and NPS receptors（NPSR） are reported as a novel endogenous arousal and anxiolytic system,but it is unclear yet whether this system is involved in anxiety-like behavior and sleep caused by sleep deprivation,and how it plays anxiolytic effect underlying the comorbid *** the present study,we demonstrate that paradoxical sleep deprivation（PSD） induced by modified multiple platform method（MMPM） for 24 h caused anxiety-like behavior,a prolonged sleep latency and subsequent paradoxical sleep（PS） rebound accompanied by an increase in electroencephalogram（EEG） theta（4.5–8.5 Hz） activities across light and dark phase in *** increase of PS after PSD was due to an increase of episode number during light phase and both episode number and duration during dark *** action of NPS（1 nmol） attenuated PSD-induced anxiety-like behavior,and altered PSD-induced sleep-wake disturbances through increasing wakefulness,and suppressing PS and EEG theta *** reduction in PS time following NPS administration during light phase was because of a decreased episode ***,sleep amount in 24 h in PSD rats given NPS was lesser than that given *** significantly enhanced NPSR mRNA expression level in the *** remarkably increased the number of Fos-ir neurons in the basolateral amygdala（BLA）,the central amygdala（CeA） and medial amygdala（MeA）.The majority of Fos-ir neurons induced by NPS also expressed *** results suggest that NPSR upregulation in the amygdala is presumably related to the PSD-induced anxiety-like behavior and sleep disturbances,and that NPS counteracts PSD-induced anxiety-like behavior and sleep disturbances possi

关键词： neuropeptide S neuropeptide S receptor paradoxical sleep deprivation anxiety-like behavior sleep-wake states amygdala

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Neuropeptide S promotes active waking through activation of histaminergic and orexinergic neurons

Neuropeptide S promotes active waking through activation of ...

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Peng ZHAO,Yu-Feng SHAO,Min ZHANG,Kun FAN,xiang-pan kong,Yi-Ping HOU~* Department of Neuroscience,Anatomy,Histology and Embryology,Key Laboratory of Preclinical Study for New Drugs of Gansu Province,School of Basic Medical Sciences,Lanzhou University,Lanzhou 730000,China

＜正＞Objective The effect of neuropeptide S（NPS） on characteristics and mechanisms in the regulation of sleep-waking cycle are unclear,though the initial findings that NPS promotes arousal associated to locomotor activity and a genuine anxiolytic-like action have recently been *** hypothesized that NPS activated posterior hypothalamic arousal systems involved histaminergic（HAergic） neurons in tuberomammilary nucleus（TMN） and orexinergic（OXergic） neurons in perifornical（PeF） and lateral hypothalamic（LH） area,where NPSR mRNA has been reported to be highly expressed,to regulate sleep-wakefulness *** present studies were designed to provide more information regarding the NPS-induced sleep-waking characteristics and potential targets in posterior hypothalamus by analyzing each state across sleep-waking cycle,especially focusing on active waking（aW） and quiet waking（qW） states since the rather unique behavioral profile challenges the common idea that anxiolytics are also sedative or that stimulants are also anxiogenic,during the 24 h period after a range dose of NPS intracerebroventricular （ICV） injection and by observing earlier genes c-fos *** Four stainless-steel screws as electroencephalogram（EEG） electrodes were screwed into the animals skull and three silver wire as electromyogram （EMG） electrodes were inserted into the dorsal cervical neck muscles to monitor the sleep-wake cycle,and the EEG and EMG signals were amplified and digitalized with a resolution of 256 and 128 Hz respectively using a CED 1401 MKⅡ.After sleep-waking recording,c-fos immunereactive（Fos-IR）,histidine decarboxylase（HDC）-IR,orexin-A （OX-A）-IR,and dual labeling of Fos-IR+HDC-IR and Fos-IR+OX-A-IR neurons were respectively labeled to identify the active sites in the brain for the NPS effects on *** The sleep-wake profile after an ICV injection of NPS at 10:00 h was examined,when rats with saline injection spend most of their time in *** given at 0.1 a

关键词： neuropeptide S sleep-wake states EEG power spectra immunohistochemistry histamine orexin

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Effect of inhibition of GABA uptake in the tuberomamillary nucleus on sleep-waking states following the ventrolateral preoptic area lesion in rats

Effect of inhibition of GABA uptake in the tuberomamillary n...

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Min ZHANG,Kun FAN,Peng ZHAO,Yu-Feng SHAO,xiang-pan kong,Qiong YAN,Yi-Ping HOU~* Department of Neuroscience,Anatomy,Histology and Embryology,Key Laboratory of Preclinical Study for New Drugs of Gansu Province,School of Basic Medical Sciences,Lanzhou University,Lanzhou 730000,China

＜正＞Objective Neurons containing gamma-aminobutyric acid（GABA） play an important role in the regulation from wakefulness to *** evidences have demonstrated that the interaction between GABAergic neurons in ventrolateral preoptic area（VLPO） and histaminergic（HA） neurons in the tuberomammillary nucleus（TMN） play a key role for regulating sleep-wakefulness *** neurons also in the TMN have been recently documented. However,GABAergic neurons in TMN have not received much attention for their role in sleep-wakefulness cycle. The present study was to investigate the effect of inhibition of GABA uptake in the TMN nucleus on sleep-waking states following the VLPO lesion and further understand the role of the GABAergic neurons in the TMN in sleep-wakefulness *** Thirty adult and male SD rats were used in this *** anesthesia,4 EEG screw electrodes were implanted into the skull and 3 flexible EMG wire electrodes were placed into the nuchal muscles. After 24 h basal record of sleep-wake cycle,microliter syringes containing ibotenic acid（IA,Sigma,***,USA） solution（10 nmol/0.5μL/side） were bilaterally injected into the VLPO for specific cells *** VLPO lesion, nipecotic acid（NPA,Sigma,***,USA）,a GABA uptake inhibitor（1 mmol/0.5μL/side）,was bilaterally injected into the TMN at 10:00 or 22:00 h for observing the changes of sleep-waking states in light period（8:00-20:00 h） and dark period（20:00-8:00 h） on day 8,10,12 and 14,*** recording,histological Nissl staining and immunohistochemical Fos and glutamate decarboxylase（GAD） staining were respectively performed for determining the loss number of cell and GABA neurons in the *** immunoreactivity（IR） and histidine decarboxylase （HDC）-IR were performed determining the distributions and morphologic characteristics of GABAergic and HAergic neurons in the ***（1） Effect of VLPO lesion on sleep-waking cycle and cortical EEG *** VLPO lesion,the sleep-wa

关键词： GABAergic neuron GABA uptake inhibitor histaminergic neuron lesion glutamate decarboxylaseimmunoreactivity histidine decarboxylase-immunoreactivity tuberomammillary nucles ventrolateral preoptic area

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circT P63-N suppresses the proliferation and metastasis of nasopharyngeal carcinoma via engaging with HSP90AB1 to modulate the YAP1/Hippo signaling pathway

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Science China Life Sciences 2025年

作者： Dan Wang Sicheng Zuo Junshang Ge Hongke Qu Jie Wu Na Yi Lei Shi Yumin Wang Yongzhen Mo Chunmei Fan Yi He pan Chen Ming Zhou Bo xiang Wei Xiong Wenjia Guo Zhaoyang Zeng Can Guo NHC Key Laboratory of Carcinogenesis and Hunan Key Laboratory of Cancer Metabolism Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine Central South University Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education Cancer Research Institute and School of Basic Medicine Sciences Central South University Furong Laboratory Cancer Research Institute Affiliated Cancer Hospital of Xinjiang Medical University Xinjiang Key Laboratory of Translational Biomedical Engineering

Circular RNAs(circRNAs) play pivotal roles in the development and progression of various diseases, including malignant tumors. However,the biological functions and the underlying mechanisms of many circRNAs remain elusive. In this study, we identified a novel circRNA,circTP63-N, generated through the splicing of exons 2–4 of the TP63 gene in nasopharyngeal carcinoma(NPC). circTP63-N was found to be downregulated in clinical samples of NPC. Both in vitro and in vivo experiments unequivocally demonstrated that circTP63-N inhibits the proliferation and metastasis of NPC cells. Further investigations revealed that circTP63-N interacted with the HSP90AB1 protein, leading to the recruitment of LATS/YAP1 proteins. This, in turn, induced phosphorylation and ubiquitination-dependent degradation of YAP1, resulting in reduced nuclear translocation of YAP1 and inhibition of the transcriptional activation of downstream oncogenic genes, including INHBA, MMP3, and CCNE2. Our findings highlight the identification of circTP63-N, a novel circRNA encoded by an important tumor-relevant gene TP63 and elucidate its molecular mechanism as a tumor suppressor in NPC. These insights offer novel potential molecular markers and therapeutic targets for the clinical diagnosis and treatment of NPC.

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压应力激活SOST/Wnt/β-catenin 通路诱导软骨终板细胞退变

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中国组织工程研究 2025年第5期29卷 951-957页

作者：项攀车艳军罗宗平苏州大学附属第一医院骨科研究所江苏省苏州市215006 南京医科大学附属苏州医院骨科与运动医学中心江苏省苏州市215000

背景:在许多可以导致椎间盘退变的因素中(衰老、营养匮乏、机械因素等),力学负荷被认为是极其重要的因素,但其机制尚不清楚。目的:探讨硬骨素和Wnt/β-catenin信号通路在压应力诱导终板软骨退变中的作用。方法:提取4周龄雄性SD大鼠软骨... 详细信息

背景:在许多可以导致椎间盘退变的因素中(衰老、营养匮乏、机械因素等),力学负荷被认为是极其重要的因素,但其机制尚不清楚。目的:探讨硬骨素和Wnt/β-catenin信号通路在压应力诱导终板软骨退变中的作用。方法:提取4周龄雄性SD大鼠软骨终板细胞,体外利用力学加载仪器对终板软骨细胞施加压应力,于压缩细胞1,3,5,7 d,采用CCK-8法测定细胞活力;Western blot、RT-qPCR及细胞免疫荧光等检查终板软骨细胞内软骨标记物(聚集蛋白聚糖、Ⅱ型胶原)、钙化相关因子(Runx2、骨钙素)、细胞外基质降解酶及信号通路基因(硬骨素、β-catenin)等表达。结果与结论:①压应力作用下,终板软骨细胞活力会随着压应力时间、强度增加而降低;②终板软骨细胞的软骨标记物(聚集蛋白聚糖、Ⅱ型胶原)表达下降,而钙化相关因子(Runx2、骨钙素)表达上升;③压应力能促进终板软骨细胞的细胞外基质降解,基质金属蛋白酶3、基质金属蛋白酶13表达量增加;④细胞内Wnt/β-catenin信号通路表达出现异常,其特异性抑制因子硬骨素伴随着β-catenin的异常积累而表达下降。结果说明:在压应力作用下,终板软骨细胞内硬骨素的表达下降,导致Wnt/β-catenin信号通路激活,促进软骨终板的钙化、退变与细胞外基质的降解,进而促进椎间盘退变。

关键词：软骨终板软骨细胞 SOST Wnt/β-catenin 压应力椎间盘退变

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Gut microbiota in cancer initiation,development and therapy

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Science China Life Sciences 2025年

作者： Ruyi Zhang xiang Zhang Harry Cheuk Hay Lau Jun Yu Institute of Digestive Disease and Department of Medicine and Therapeutics State Key Laboratory of Digestive DiseaseLi Ka Shing Institute of Health SciencesCUHK Shenzhen Research InstituteThe Chinese University of Hong Kong

Cancer has long been associated with genetic and environmental factors,but recent studies reveal the important role of gut microbiota in its initiation and *** 13%of cancers are linked to infectious agents,highlighting the need to identify the specific microorganisms *** microbiota can either promote or inhibit cancer growth by influencing oncogenic signaling pathways and altering immune *** can lead to cancer,while certain probiotics and their metabolites may help reestablish microecological balance and improve anti-tumor immune *** into targeted approaches that enhance therapy with probiotics is ***,the effects of probiotics in humans are complex and not yet fully ***,methods to counteract harmful bacteria are still in *** clinical trials also indicate that modifying gut microbiota may help manage side effects of cancer *** research is crucial to understand better how gut microbiota can be used to improve cancer prevention and treatment outcomes.

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Controlled Radical Polymerization:from Oxygen Inhibition and Tolerance to Oxygen Initiation

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Chinese Journal of Polymer Science 2021年第9期39卷 1084-1092页

作者： Ning Li xiang-Cheng pan State Key Laboratory of Molecular Engineering of Polymers Department of Macromolecular ScienceFudan UniversityShanghai 200438China

Molecular oxygen is a radical scavenger in both conventional and controlled radical polymerization(CRP),resulting in many time-consuming methods for physically removing oxygen before the *** approaches have been developed to have oxygen toleranee by chemically consuming or converting molecular oxygen into non-initiating species to address this ***,we propose another approach called oxygen initiation that directly transforms molecular oxygen into the initiating carbon radical in *** feature article summarizes our rece nt developments in this *** n-initiated reversible additi on-fragmentation transfer(RAFT)polymerization has been successfully conducted using oxygen and trialkylborane as co-initiators under the ambient conditions and atmosphere without any prior degassed *** gas-triggered initiation provides the opportunity for spatiotemporal control of the polymerization by molecular oxygen or *** synthesized alkylborane compounds could derive the predesigned structure of the initiating alkyl radical to minimize the side reacti ons and free polymer chai ns,achievi ng the synthesis of ultra-high molecular weight *** challe nges and perspectives are also discussed in the end.

关键词： Oxygen Radical polymerization Controlled Alkylborane RAFT

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GSM-MRF based classification approach for real-time moving object detection

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2008年第2期9卷 250-255页

作者： xiang pan Yi-jun WU Institute of Information and Communication Engineering Zhejiang University Hangzhou 310027 China

Statistical and contextual information are typically used to detect moving regions in image sequences for a fixed *** this paper,we propose a fast and stable linear discriminant approach based on Gaussian Single Model(GSM)and Markov Random Field(MRF).The performance of GSM is analyzed first,and then two main improvements corresponding to the drawbacks of GSM are proposed:the latest filtered data based update scheme of the background model and the linear classification judgment rule based on spatial-temporal feature specified by *** results show that the proposed method runs more rapidly and accurately when compared with other methods.

关键词： Moving object detection Markov Random Field (MRF) Gaussian Single Model (GSM) Fisher Linear Discriminant Analysis (FLDA)

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