Neuropeptide S facilitates mice olfactory function through activation of cognate receptor-expressing neurons in the olfactory cortex
作者单位：Department of NeuroscienceAnatomyHistology and EmbryologyKey Laboratory of Preclinical Study for New Drugs of Gansu ProvinceSchool of Basic Medical SciencesLanzhou University
会议名称：《中国神经科学学会第十届全国学术会议》
会议日期：2013年
学科分类：0710[理学-生物学] 07[理学] 071006[理学-神经生物学]
基 金：supported by the National Natural Science Foundation of China(30670677,81071076 and81171254) grant from the Key National S&T Program "Major New Drug Development" of the Ministry of Science and Technology of China(2009ZX09503-017)
关 键 词：neuropeptide S neuropeptide S receptor olfactory function olfactory cortex [D-Val~5]NPS c-Fos
摘 要：Objective Neuropeptide S(NPS) is a newly identified neuromodulator located in the brainstem and regulates various biological functions by selectively activating the NPS receptors(NPSR).High level expression of NPSR mRNA in the olfactory cortex suggests that NPS-NPSR system might be involved in the regulation of olfactory *** present study was undertaken to investigate the effects of intracerebroventricular(i.c.v.) injection of NPS or co-injection of NPSR antagonist on the olfactory behaviors,food intake,and c-Fos expression in olfactory cortex in *** Olfactory abilities in mice were gauged using the buried food test(to assess the ability of detecting volatile odors) and olfactory habituation/dishabituation test(to assess the ability of detecting and distinguishing the same and different odors).Food intake test was used to clarify the relationship between olfaction and ingestion in fasted32 h mice after *** of *** further identify potential neuronal targets of NPS in the olfactory cortex,NPS-induced Fos immunereactive(-ir) neurons were analyzed using ex vivo immunohistochemistry,and the presence of NPSR in these neurons was examined using dual-immunofluorescence ***(1) In comparison with vehicle-treated mice,*** of 0.1,0.5,and 1 nmol of NPS significantly reduced the latency to find the buried food from 73.43 ± 11.77 s to 35.74 ± 5.37 s(P 0.001),12.72 ± 1.34 s(P 0.001) and 24.61 ± 5.04 s(P 0.001),*** the three doses,0.5 nmol NPS reduced the latency most(P 0.001 and P 0.05 compared with vehicle and 0.1 nmol NPS,respectively).In fact,high dose(1 nmol) of NPS insignificantly reduced the latency as compared to 0.1 nmol NPS(35.74± 5.37 s vs.24.61 ± 5.04 s,P = 0.24).(2) Central administration of vehicle induced a habituation to water(P 0.05) and vanilla(P 0.001),and a dishabituation to vanilla(P 0.001).Mice administered NPS at 0.1 nmol were able to distinguish almond and vanilla