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Differentiation and tumorigenicity of neural stem cells from human cord blood mesenchymal stem cells

Neural Regeneration Research 2009年第10期4卷 769-774页

作者： jing xiang Changming wang jingzhou wang Department of Neurology Research Institute of Surgery Daping Hospital Third Military Medical University of Chinese PLA Chongqing 400042 China

BACKGROUND： Mesenchymal stem cells （MSCs） are capable of differentiating into a variety of tissues and exhibit low immunogenicity. OBJECTIVE： To investigate isolation and in vitro cultivation methods of human cord blood MSCs, to observe expression of neural stem cell （NSC） marker mRNA under induction, and to detect tumorigenicity in animals. DESIGN, TIME AND SETTING： A cell biological, in vitro trial and a randomized, controlled, in vivo experiment were performed at the Department of Neurology, Daping Hospital at the Third Military Medical University of Chinese PLA from August 2006 to May 2008. MATERIALS： Umbilical cord blood was collected from full-term-delivery fetus at the Department of Gynecology and Obstetrics of Daping Hospital, China. Eighteen BALB/C nu/nu nude mice were randomly assigned to three groups： back subcutaneous, cervical subcutaneous, and control, with 6 mice in each group. METHODS： Monocytes were isolated from heparinized human cord blood samples by density gradient centrifugation and then adherent cultivated in vitro to obtain MSC clones. After the cord blood MSCs were cultured for 7 days with nerve growth factor and retinoic acid to induce differentiation into NSCs, the cells （adjusted density of 1 × 10^7/mL） were prepared into cell suspension. In the back subcutaneous and cervical subcutaneous groups, nude mice were hypodermically injected with a 0.5-mL cell suspension into the back and cervical regions, respectively. In the control group, nude mice received a subcutaneous injection of 0.5 mL physiological saline into the back or cervical regions, respectively. MAIN OUTCOME MEASURES： Cellular morphology was observed by inverted microscopy, cultured cord blood MSCs were examined by flow cytometry, expression of nestin and musashi-1 mRNA was detected by reverse-transcriptase polymerase chain reaction prior to and after induction, and tumorigenicity following cord blood MSC transplantation was assayed by hematoxylin-eosin staining. RESULTS： Follo

关键词： cord blood mesenchymal stem cells neural stem cells induced differentiation nestin tumorigenicity

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A novel and efficient route for the preparation of atorvastatin

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Chinese Chemical Letters 2011年第10期22卷 1159-1162页

作者： Jian Gao Yang Hui Guo Ya Ping wang xiang jing wang Wen Sheng xiang Department of Biochemical Engineering Northeast Agricultural University Harbin 150030 China Zhejiang Hisun Pharmaceutical Co. Ltd. Taizhou 318000 China

A novel and efficient synthetic method of atorvastatin was *** key step of the synthesis was the construction of the olefin linkage between the chiral side chain and skeleton via a Horner-Wadsworth-Emmons reaction,resulting in the advanced intermediate of atorvastatin under hydrogenation of the olefin over Pd/*** novel method is more useful for the practical synthesis of atorvastatin than its document reported methods.

关键词： Atorvastatin Horner-Wadsworth-Emmons reaction Synthesis Intermediate

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Novel synthesis of 2-butyl-5-chloro-3H-imidazole-4-carbaldehyde:A key intermediate of Losartan

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Chinese Chemical Letters 2009年第3期20卷 269-270页

作者： Hai Bo Sun Guo Jun Zheng Ya Ping wang xiang jing wang Wen Sheng xiang College of Life Science Northeast Agricultural University Harbin 150030 China Zhejiang Hisun Pharmaceutical Co. Ltd. Taizhou 318000 China

A novel method for synthesis of 2-butyl-5 -chloro-3H-imidazole-4-carbaldehyde 2, a key intermediate of Losartan was reported. The compound 2 was synthesized from starting material dimethyl malonate 6 and n-valeronitrile 8 by six steps with an overall yield of 40%. The key step including the reaction of compound 5 with POCl3/DMF followed by hydrolysis to give compound 2 with the yield of 68%.

关键词： Losartan Antihypertensive Angiotensin II 2-Butyl-5-chloro-3H-imidazole-4-carbaldehyde

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Practical synthesis of methyl(E)-2-(3-(3-(2-(7-chloro-2- quinolinyl)ethenyl)phenyl)-3-oxopropyl)benzoate,a key intermediate of Montelukast

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Chinese Chemical Letters 2012年第5期23卷 518-520页

作者： Liang He Yang Hui Guo Ya Ping wang xiang jing wang Ji Zhang Wen Sheng xiang Department of Biochemical Engineering Northeast Agricultural UniversityHarbin 150030China Zhejiang Hisun Pharmaceutical Co. Ltd.Taizhou 318000China

A novel and practical synthetic route is presented for the preparation of methyl-（E）-2-（3-（3-（2-（7-chloro-2-quinolinyl）ethenyl） phenyl）-3-oxopropyl）benzoate,the key intermediate of Montelukast,a leukotriene *** main diarylpropane framework was prepared via a polarity conversation reaction resulting in an acyl anion equivalent followed by a nucleophilic substitution *** overall yield of this approach was 61%.This method is simple for operation and suitable for industrial production.

关键词： Montelukast Synthesis Intermediate Polarity conversion

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Improved and efficient synthesis of Maxacalcitol

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Chinese Chemical Letters 2014年第5期25卷 771-774页

作者： Shi Feng Li-Fei Cui He-Geng Wei Guo-Jun Zheng Ya-Ping wang xiang-jing wang Ji Zhang Wen-Sheng xiang College of Science Northeast Agricultural University School of Life Science Northeast Agricultural University Harbin FRP Institute Zhejiang Hisun Pharmaceutical Co. Ltd.

Maxacalcitol,the 22-oxa-derivative of 1a,25-dihydroxyvitamin D3,has been used as an antihyperparathyroidism and antipsoriatic *** this paper,an alternative synthetic route has been developed using commercially available vitamin D2as the starting *** addition,as a key intermediate,the asymmetric synthesis of 20（S）-alcohol 14 with the chiral auxiliary（R-CBS） was described for the first *** new synthetic route afforded Maxacalcitol with improved overall yield（13.9%） in eleven steps.

关键词： Vitamin D3 derivative Maxacalcitol Synthesis Asymmetric reduction

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Comparison of laparoscopic vs open liver lobectomy(segmentectomy) for hepatocellular carcinoma

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World Journal of Gastroenterology 2011年第42期17卷 4725-4728页

作者： Ben-Shun Hu Ke Chen Hua-Min Tan xiang-Ming Ding jing-wang Tan Hepato-bilio-pancreatic Surgery Department Northern Jiangsu People＇s Hospital Yangzhou 225001 Jiangsu Province China Hua-Min Tan General Surgery Department Fuzhou General Hospital of Nanjing Military Command Fuzhou 350000 Fujian Province China

AIM:To investigate the effects of laparoscopic hepatectomy for the treatment of hepatocellular carcinoma(HCC) .METHODS:From 2006 to January 2011,laparoscopic hepatectomies were performed on 30 cases of HCC at Northern Jiangsu People's Hospital. During this sametime period,30 patients elected to undergo conventional open hepatectomy over laparoscopic hepatectomy at the time of informed consent. The degree of invasiveness and outcomes of laparoscopic hepatectomy compared to open hepatectomy for HCC were ***:Both groups presented with similar bloodloss amounts,operating times and complications. Patients in the laparoscopic hepatectomy group started walking and eating significantly earlier than those inthe open hepatectomy group,and these more rapid recoveries allowed for shorter hospitalizations. There were no significant differences between procedures insurvival ***:Laparoscopic hepatectomy is beneficial for patient quality of life if the indications are appropriately based on preoperative liver function and the location and size of the HCC.

关键词： Laparoscopy Hepatocellular carcinoma Liver resection Liver Iobectomy

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Infrared spectra of soluble polytoluidines

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Acta Chimica Sinica English Edition 1989年第1期7卷 53-58页

作者： wang Li-xiang jing Xia-Bin wang Fo-Song Changchun Institute of Applied Chemistry,Academia Sinica Changchun Institute of Applied Chemistry Academia Sinica

IR spectra of soluble poly-o-toluidine(POT)and poly-m-toluidine(PMT)have been *** assignment of their IR spectra is given by comparing their spectra in HCI and I_2 doped states and in subsequently NH_3 dedoped state w... 详细信息

关键词： HCI

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SNR WALL AND COOPERATIVE SPECTRUM SENSING IN COGNITIVE RADIO UNDER NOISE UNCERTAINTY

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Journal of Electronics(China) 2010年第5期27卷 611-617页

作者： wang Haijun Su Xin Xu Yi Chen xiang wang jing Tsinghua National Laboratory for Information Science and Technology Tsinghua University Beijing 100084China

Cooperative energy spectrum sensing has been proved effective to detect the spectrum holes in Cognitive Radio(CR).However,its performance may suffer from the noise uncertainty,which is portrayed by the SNR wall in some *** this paper we analyze the spectrum sensing per-formance under noise uncertainty and find an alternative approach to obtain the SNR *** the average SNR wall is proposed to illustrate the statistically average impact of noise *** addition,the cooperative sensing performance under noise uncertainty with AND rule is *** and numerical results show that cooperative sensing can significantly improve the sensing performance under the condition of noise uncertainty.

关键词： Cooperative spectrum sensing Noise uncertainty SNR wall

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Er^(3+)掺杂调控NaY(WO_4)_2:Dy^(3+)的上转换发光性能

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无机材料学报 2018年第5期33卷 521-527页

作者：史忠祥王晶关昕大连交通大学辽宁省无机超细粉体制备及应用重点实验室大连116028

采用水热法合成出Dy^(3+),Er^(3+)共掺杂NaY(WO_4)_2荧光粉。利用XRD和SEM对样品的晶体结构及形貌进行表征。研究了上转换发光性能和Dy^(3+)的~4F_(9/2)→~6H_(13/2)跃迁的荧光衰减曲线,并讨论了Dy^(3+)→Er^(3+)的能量传递过程。结果表... 详细信息

采用水热法合成出Dy^(3+),Er^(3+)共掺杂NaY(WO_4)_2荧光粉。利用XRD和SEM对样品的晶体结构及形貌进行表征。研究了上转换发光性能和Dy^(3+)的~4F_(9/2)→~6H_(13/2)跃迁的荧光衰减曲线,并讨论了Dy^(3+)→Er^(3+)的能量传递过程。结果表明:所有合成的样品均具有NaY(WO_4)_2四方相结构;加入聚乙二醇(PEG-2000)作为表面活性剂,可得到分散性良好的微米针状球;在780 nm近红外光激发下,观察到了480 nm蓝光、576 nm黄光、531 nm及554 nm绿光发射峰,其中蓝光和黄光分别来自Dy^(3+)的~4F_(9/2)→~6H_(15/2)与~4F_(9/2)→~6H_(13/2)跃迁,绿光发射由Er^(3+)的~2H_(11/2)→~4I_(15/2)和~4S_(3/2)→~4I_(15/2)跃迁产生。通过研究荧光光谱和荧光衰减曲线,证实了Dy^(3+)→Er^(3+)的能量传递过程,且该能量传递的机制为电偶极–电偶极相互作用。通过调节NaY(WO_4)_2:1.0mol%Dy^(3+),xmol%Er^(3+)荧光粉中Er^(3+)的浓度,可实现由准白光到蓝光的转变。

关键词：上转换发光 NaY(WO4)2:Dy^(3+) 聚乙二醇能量传递

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县域视角下的贫困、金融发展与经济增长——基于系统GMM方法的再检验

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经济理论与经济管理 2017年第1期37卷 84-94页

作者：王仁祥王婧武汉理工大学经济学院

本文以内生区域金融发展理论为基础构建动态面板模型,同时引入贫困因素进一步识别区域金融与经济的非线性关系,将实证对象细化到县域层面,运用系统GMM方法检验贫困与非贫困县域金融对经济增长影响的差异。本文研究发现,信贷层面上的金... 详细信息

本文以内生区域金融发展理论为基础构建动态面板模型,同时引入贫困因素进一步识别区域金融与经济的非线性关系,将实证对象细化到县域层面,运用系统GMM方法检验贫困与非贫困县域金融对经济增长影响的差异。本文研究发现,信贷层面上的金融发展对经济增长的推动效应普遍存在,而储蓄层面金融发展的效应则存在地区差异,非贫困县的储蓄与经济无关,贫困县的储蓄与经济增长负相关。因此本文指出,区域信贷配给和区域信贷创造理论更适于解释贫困地区的金融发展问题,贫困县域储蓄的负效应是本地储蓄外流和转化效率低下的结果,与我国银行业跨区域经营机构占主导的现实有关。

关键词：区域金融发展贫困县域银行跨区域经营系统GMM

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