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TGFβ signaling hyperactivation-induced tumorigenicity during the derivation of neural progenitors from mouse ESCs

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Journal of Molecular Cell Biology 2018年第3期10卷 216-228页

作者： xianfa yang Ran Wang Xiongjun Wang Guoqing Cai Yun Qian Su Feng Fangzhi Tan Kun Chen Ke Tang Xingxu Huang Naihe Jing Yunbo Qiao State Key Laboratory of Cell Biology CAS Center for ExceUence in Molecular Cell Science Shanghai Institute of Biochemistry and Cell Biology Chinese Academy of Sciences University of Chinese Academy of Sciences Shanghai 200031 China School of Life Science and Technology ShanghaiTech University Shanghai 201210 China Precise Genome Engineering Center School of Life Sciences Guangzhou University Guangzhou 510006 China

Clinical therapies of pluripotent stem cells （PSCs）-based transplantation have been hindered by frequent development of terato- mas or tumors in animal models and clinical patients. Therefore, clarifying the mechanism of carcinogenesis in stem cell therapy is of great importance for reducing the risk of tumorigenicity. Here we differentiate Oct4-GFP mouse embryonic stem cells （mESCs） into neural progenitor cells （NPCs） and find that a minority of Oct4＋ cells are continuously sustained at Oct4＋ state. These cells can be enriched and proliferated in a standard ESC medium. Interestingly, the differentiation potential of these enriched cells is tightly restricted with much higher tumorigenic activity, which are thus defined as differentiation-resistant ESCs （DR-ESCs）. Transcriptomic and epigenomic analyses show that DR-ESCs are characterized by primordial germ cell-like gene sig- natures （Dazl, Rec8, Stro8, BUmp1, etc.） and specific epigenetic patterns distinct from mESCs. Moreover, the DR-ESCs possess germ cell potential to generate Sycp3＋ haploid cells and are able to reside in sperm-free spermaduct induced by busulfan. Finally, we find that TGFβ signaling is overactivated in DR-ESCs, and inhibition of TGFβ signaling eliminates the tumorigenicity of mESC-derived NPCs by inducing the full differentiation of DR-ESCs. These data demonstrate that these TGFβ-hyperactivated germ ceU-like DR-ESCs are the main contributor for the tumorigenicity of ESCs-derived target cell therapy and that inhibition of TGFβ signaling in ESC-derived NPC transplantation could drastically reduce the risk of tumor development. Keywords： embryonic stem cells, differentiation-resistant ESCs, tumorigenicity, germ cell, TGFβ signaling

关键词： embryonic stem cells differentiation-resistant ESCs tumorigenicity germ cell TGFβ signaling

Synthesis of photostable near-infrared sulfone-rhodamines for photoacoustic imaging-guided photothermal therapy

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Chinese Chemical Letters 2024年第3期35卷 369-372页

作者： Huiyu Si Dongjuan Wang xianfa Du Xin Zhou Department of Chemistry College of Chemistry and Chemical EngineeringQingdao UniversityQingdao 266071China Department of Orthopedics the Affiliated Hospital of Qingdao UniversityQingdao 266071China

Photothermal therapy(PTT)is a cutting-edge cancer treatment that can kill cancer cells in hypoxic environments without relying on *** of the ideal photothermal agents with a high absorption coefficient in the near-infrared region,and a high excellent photothermal conversion efficiency is of great ***-Rhodanmine dye has showed an impressive absorption wavelength over 700 nm,but suffered from a stability *** this study,we synthesized five sulfone rhodamines and investigated the substitution effects on ***_(2)R2 showed high stability and strong absorbance at 714 nm with an excellent photothermal conversion efficiency of 53.06%,making it suitable for accurate photoacoustic imaging-guided photothermal therapy in vivo.

关键词： Photothermal therapy Sulfone rhodamine Near-Infrared Photoacoustic Imaging

Generation of functional posterior spinal motor neurons from hPSCs-derived human spinal cord neural progenitor cells

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Cell Regeneration 2023年第1期12卷 317-337页

作者： He Jax Xu Yao Yao Fenyong Yao Jiehui Chen Meishi Li xianfa yang Sheng Li Fangru Lu Ping Hu Shuijin He Guangdun Peng Naihe Jing State Key Laboratory of Cell Biology CAS Center for Excellence in Molecular Cell ScienceShanghai Institute of Biochemistry and Cell BiologyChinese Academy of SciencesShanghai 200031China University of Chinese Academy of Sciences BeijingChina School of Life Science and Technology ShanghaiTech UniversityShanghai 201210China Center for Cell Lineage and Development CAS Key Laboratory of Regenerative BiologyGuangdong Provincial Key Laboratory of Stem Cell and Regenerative MedicineGIBH‑HKU Guangdong‑Hong Kong Stem Cell and Regenerative Medicine Research CentreGuangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou 510530China Guangzhou Laboratory/Bioland Laboratory Guangzhou 510005China Xinhua Hospital affiliated to Shanghai Jiao Tong University School of Medicine Shanghai 20023China Institute for Stem Cell and Regeneration Chinese Academy of SciencesBeijing 100101China Center for Cell Lineage and Atlas Bioland LaboratoryGuangzhou 510005China

Spinal motor neurons deficiency results in a series of devastating disorders such as amyotrophic lateral sclerosis(ALS),spinal muscular atrophy(SMA)and spinal cord injury(SCI).These disorders are currently incurable,while human pluripotent stem cells(hPSCs)-derived spinal motor neurons are promising but suffered from inappropriate regional identity and functional immaturity for the study and treatment of posterior spinal cord related *** this study,we have established human spinal cord neural progenitor cells(hSCNPCs)via hPSCs differentiated neuromesodermal progenitors(NMPs)and demonstrated the hSCNPCs can be continuously expanded up to 40 *** can be rapidly differentiated into posterior spinal motor neurons with high *** functional maturity has been examined in ***,a co-culture scheme which is compatible for both neural and muscular differentiation is developed to mimic the neuromuscular junction(NMJ)formation in ***,these studies highlight the potential avenues for generating clinically relevant spinal motor neurons and modeling neuromuscular diseases through our defined hSCNPCs.

关键词： Motor neuron Motor neuron differentiation Neuromesodermal progenitors Neuromuscular junction

Progress in the application of graphene material in oilfield chemistry:A review

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Petroleum 2024年第2期10卷 175-190页

作者： Jinsheng Sun Yuanwei Sun Yong Lai Li Li Gang yang Kaihe Lv Taifeng Zhang xianfa Zhang Zonglun Wang Zhe Xu Zhiwen Dai Jingping Liu School of Petroleum Engineering China University of Petroleum(East China)Qingdao266580China CNPC Engineering Technology R&D Company Limited Changping10206China Nanchong Vocational and Technical College Nanchong637131China Oil&Gas Technology Research Institute of Changqing Oilfield Company Xi'an710000China CNPC Greatwall Drilling Fluids Company Panjin124000China

Graphene is a single atom thick crystal composed of carbon *** is the lightest,thinnest,strongest material that conducts heat and electricity well *** terms of application,by introducing oxygen-containing groups,graphene can be well dispersed in solvents,can be chemically modified and functionalized,or connected with other electroactive substances through covalent bond or non-covalent bond to form composite materials,which is conducive to further processing and *** application of graphene in oilfield chemistry started late,but developed *** has played an active role in drilling fluid,cementing fluid,fracturing fluid,displacement fluid and other oilfield working *** can enhance the temperature and salt resistance of working fluid and improve the effect of working *** this paper,several directions of graphene applications in oilfield chemistry,such as modified graphene,graphene copolymers and graphene nanoparticles,are reviewed in detail from the synthesis methods,action mechanisms and effects of graphene and its derivatives,and the frontier cases at this stage are *** the basis of the existing research,suggestions for the development direction of graphene materials in oilfield chemistry are given for a variety of graphene materials,aiming to provide guidance for the application of graphene in oilfield chemistry.

关键词： Oilfield chemistry Graphene Action mechanism Application Prospect

新型节能精密铸造粘接剂——ZF-801型快干硅溶胶

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特种铸造及有色合金 2009年第1期29卷 53-54页

作者：李毓飞杨先发吴仁智武汉市智发科技开发有限公司

通过对现行精密铸造制壳工艺优缺点进行比较,提出一种新型节能精密铸造粘接剂——ZF-801型快干硅溶胶。分析了ZF-801型快干硅溶胶型壳的制壳时间、铸件尺寸精度、表面质量、常温强度、高温强度、残留强度等方面的状况,发现ZF-801型快干... 详细信息

通过对现行精密铸造制壳工艺优缺点进行比较,提出一种新型节能精密铸造粘接剂——ZF-801型快干硅溶胶。分析了ZF-801型快干硅溶胶型壳的制壳时间、铸件尺寸精度、表面质量、常温强度、高温强度、残留强度等方面的状况,发现ZF-801型快干硅溶胶是符合较少污染物排放要求,且成本适中的精密铸造制壳专用粘结剂。

关键词：快干硅溶胶节能精密铸造制壳工艺

Application status and prospect of ionic liquids in oilfield chemistry

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Petroleum 2024年第1期10卷 11-18页

作者： Jinsheng Sun Zhuoyang Xiu Li Li Kaihe Lv xianfa Zhang Zonglun Wang Zhiwen Dai Zhe Xu Ning Huang Jingping Liu School of Petroleum Engineering China University of Petroleum(East China)Qingdao266580China CNPC Engineering Technology R&D Company Limited Changping10206China Oil&Gas Technology Research Institute of Changqing Oilfield Company Xi'an710000China

The ionic liquid,as a new treatment agent,has been increasingly applied in oil fields due to its strong temperature resistance,good solubility and high surface *** this paper,we systematically discuss the action mechanism and application effect of ionic liquids in oilfield *** liquids can inhibit shale hydration expansion and reduce fluid loss through adsorption and intercalation,inhibit the formation of natural gas hydrate through imidazole five-membered ring structure as a space barrier,reduce viscosity of heavy oil by breaking chemical bonds of heavy oil macromolecules and charge transfer,improve oil displacement efficiency by forming ions pairs with carboxyl groups in crude oil,demulsify by forming channels between dispersed water droplets,acidify the formation by reacting with water to produce acid,interacts with organic material through weak hydrogen bonds and extracts it from oilfield wastewater,desulphurize by inserting sulfide molecules into the“stack”structure and form liquid inclusion complex,inhibit corrosion by forming a protective film on the metal *** on the above aspects,the development direction of ionic liquids is *** application of ionic liquids in oilfield chemistry is still in its *** is urgent to fully explore the application performance of ionic liquids in oilfield chemistry,which also provides theoretical and technical supports for efficient reservoir development.

关键词： Ionic liquids Oilfield chemistry Shale inhibitor Drilling fluid

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山东省邹平县玉米黄斑病发生因素探析

作物杂志 2008年第4期 77-80页

作者：杨先法于传策山东省邹平县农业局山东省邹平县气象局

黄斑病是近年来玉米生产的重要病害,其危害程度超过大斑病和小斑病。本研究从品种、气象、栽培、环境等多方面探析了其发病规律。结果表明:年际间、品种间发病程度差别较大,不同播种基础、不同种植方式、不同地域也存在较大差异,应重点... 详细信息

黄斑病是近年来玉米生产的重要病害,其危害程度超过大斑病和小斑病。本研究从品种、气象、栽培、环境等多方面探析了其发病规律。结果表明:年际间、品种间发病程度差别较大,不同播种基础、不同种植方式、不同地域也存在较大差异,应重点采用农艺措施进行预防。

关键词：玉米黄斑病发生因素

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烧结钕铁硼磁体溅射渗镝工艺与磁性能研究

材料导报 2017年第4期31卷 17-20,35页

作者：李家节郭诚君周头军饶先发周慧杰杨斌江西理工大学工程研究院赣州341000 江西理工大学材料科学与工程学院赣州341000 虔东稀土集团股份有限公司赣州341000

采用直流磁控溅射的方法,在烧结Nd-Fe-B磁体表面制备了Dy薄膜,对比研究了N35烧结态与回火态磁体晶界扩散后组织形貌与性能的变化。N35烧结态与回火态磁体经溅射渗Dy处理后,在剩磁仅降低0.009T和0.03T的情况下,矫顽力大幅度提高,分别提高... 详细信息

采用直流磁控溅射的方法,在烧结Nd-Fe-B磁体表面制备了Dy薄膜,对比研究了N35烧结态与回火态磁体晶界扩散后组织形貌与性能的变化。N35烧结态与回火态磁体经溅射渗Dy处理后,在剩磁仅降低0.009T和0.03T的情况下,矫顽力大幅度提高,分别提高了708.44kA/m和665.46kA/m,渗Dy处理后磁体中的Dy元素平均质量分数增加不超过0.4%。SEM和EDS能谱的分析结果表明,晶界组织形貌的改善和(Nd,Dy)2Fe14B外延层的形成是矫顽力提升的主要原因。EPMA元素面分布结果显示,Dy主要富集在富Nd相处,三叉型富Nd相处Dy含量最高,而Dy没有扩散到主相晶粒内部,不会导致剩磁大幅度降低,从而有效提高了磁体的综合磁性能。

关键词：磁控溅射晶界扩散 Dy元素烧结钕铁硼

大鼠脊髓横断后不同时期膀胱顺应性和形态学变化

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中华实验外科杂志 2016年第6期33卷 1458-1460页

作者：赵耀瑞郜海涛刘奔杨宇明杨福江杨宪法李航武装警察部队后勤学院附属医院泌尿外科天津300162 天津医科大学第二医院泌尿外科天津市泌尿外科研究所 300211 天津医科大学 300070

目的观察大鼠脊髓横断致神经源性膀胱不同时间点膀胱顺应性和形态学变化。方法将72只雌性Wistar大鼠随机分为假手术6周组（A组）、脊髓横断6周组（B组）、假手术12周组（C组）和脊髓横断12周组（D组）。两脊髓横断组行脊髓胸10水平完全... 详细信息

目的观察大鼠脊髓横断致神经源性膀胱不同时间点膀胱顺应性和形态学变化。方法将72只雌性Wistar大鼠随机分为假手术6周组（A组）、脊髓横断6周组（B组）、假手术12周组（C组）和脊髓横断12周组（D组）。两脊髓横断组行脊髓胸10水平完全横断，假手术组只暴露不横断脊髓。分别于术后6周和12周行尿动力学检查，膀胱标本行苏木素一伊红（HE）染色、Verhoeff-VanGieson染色，分别观察膀胱壁平滑肌、胶原纤维及弹性纤维比例的变化。结果与对照组比较，脊髓横断组大鼠出现运动和排尿功能障碍，B组膀胱顺应性较对照组升高，D组顺应性较对照组降低，差异均有统计学意义（P〈0．05），脊髓横断组肌纤维走行紊乱与胶原纤维比例增加均随损伤时间延长逐渐加重；D组与B组比较，膀胱顺应性降低[（0．046±0．023）ml／cmH2O比（0．146±0．107）ml／cmH2O（1cmH2O=0．098kPa），P〈0．01]，膀胱壁肌纤维横截面积比例降低[（46．137±4．085）％比（55．260±6．873）％，P〈0．01]而胶原纤维比例增加[（19．757±2．071）％比（11．104±1．125）％，P〈0．01]。结论大鼠脊髓横断后，随着时间的延长，膀胱顺应性呈先升高后降低的趋势，膀胱壁纤维化尤其是胶原纤维的比例增加可能是造成膀胱顺应性减低的病理基础。

关键词：脊髓横断神经源性膀胱膀胱顺应性胶原纤维