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Genetic analysis and gene mapping of a dominant presenescing leaf gene PSL3 in rice (Oryza sativa L.)

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Chinese Science Bulletin 2010年第23期55卷 2517-2521页

作者： FANG LiKui LI YunFeng GONG XiaoPing SANG xianchun LING YingHua wang XiaoWen CONG YunFei HE GuangHua Rice Research Institute Key Laboratory of Biotechnology and Crop Quality Improvement Ministry of Agriculture Southwest University Chongqing 400716 China

Leaf senescence as an active process is essential for plant survival and ***,premature senility is harmful to agricultural *** this study,a rice mutant,named as psl3 (presescing leaf 3) isolated from EMS-treated Jinhui 10,displays obvious premature senility features both in morphological and physiological *** analysis showed that mutant trait was controlled by a single dominant gene (PSL3),which was located on rice chromosome 7 between SSR marker c7sr1 and InDel marker ID10 with an interval of 53.5 *** result may be useful for the isolation of the PSL3 gene.

关键词：水稻叶片遗传分析基因定位早衰 SSR标记基因分离叶片衰老农业生产

Evaluation of strategy for analyzing mouse liver plasma membrane proteome

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Science China(Life Sciences) 2007年第6期50卷 731-738页

作者： CHEN Ping ZHANG LiJun LI XuanWen wang XiE CAO Rui LIU Zhen XIONG JiXian PENG Xia WEI YingJuan YING XingFeng wang xianchun LIANG SongPing College of Life Sciences Hunan Normal UniversityChangsha 410081China

Plasma membrane(PM)proteome is one of the major subproteomes present in the cell,and is very important in liver *** the present work,C57 mouse liver PM was purified by density-gradient *** purified PM was verified by electron microscope analysis and Western *** results showed that the PM was enriched by more than 20-fold and the contamination of mitochondria was reduced by 2-fold compared with the homogenization *** were separated by 2DE and 1DE,trypsin-digested and submitted to ESI-Q-TOF and MALDI-TOF-TOF mass spectrometry or directly digested in solution and analyzed by LC-ESI ion trap mass *** all,547 non-redundant mouse liver PM proteins were identified,of which 34%contributed to plasma membrane or plasma membrane-related *** study optimized and evaluated the HLPP plasma membrane proteome analysis method and made a systematic analysis on PM proteome.

关键词： liver plasma membrane proteomics mass spectrometry 2DE shotgun 1DE

How China＇s Fiscal Policy Contributes to Investment and Economic Growth： Experience and Lessons

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China Economist 2013年第6期8卷 4-17页

作者： Xu xianchun wang Baobin Xu Xiongfei National Bureau of Statistics Beijing China

This paper （1） discusses how the two indicators used in official statistics of China, namely total investment in fixed assets and the gross fixed capital formation, reflect changes infixed asset investment and their relationship, （2） analyzes the growth of fixed asset investment and its contribution to changes of economic growth rate since the reform and opening-up, and （3） explains how fiscal policy impacts the growth of fixed asset investment and analyzes in detail the impacts of the two rounds of contractionary fiscal policy, two rounds of expansionary fiscal policy and one round of neutral fiscal policy on China＇ s fixed asset investment growth since reform and opening-up using full and accurate data. Practice shows that the impact of fiscal policy on China＇ s fixed asset investment is direct and obvious, yet sometimes too drastic. In the future, fiscal policy should be used in alignment with other economic policies with appropriate intensity and timing so that it will help stabilize the growth of fixed asset investment.

关键词： total investment in fixed assets gross fixed capital formation fiscal policy growth performance

Genetic analysis and molecular mapping of a novel gene for zebra mutation in rice (Oryza sativa L.)

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Journal of Genetics and Genomics 2009年第11期36卷 679-684页

作者： Qiushi wang,xianchun Sang,Yinghua Ling,Fangming Zhao,Zhenglin Yang,Yunfeng Li,Guanghua He Key Laboratory of Biotechnology and Crop Quality Improvement,Ministry of Agriculture Rice Research Institute,Southwest University,Chongqing 400716,China [a]Key Laboratory of Biotechnology and Crop Quality Improvement Ministry of Agriculture Rice Research Institute Southwest University Chongqing 400716 China

A novel zebra mutant, zebra-15, derived from the restorer line JinhuilO （Oryza sativa L. ssp. indica） treated by EMS, displayed a distinctive zebra leaf from seedling stage to jointing stage. Its chlorophyll content decreased （55.4%） and the ratio of Chla/Chlb increased （90.2%） significantly in the yellow part of the zebra-15, compared with the wild type. Net photosynthetic rate and fluorescence kinetic parameters showed that the decrease of chlorophyll content significantly influenced the photosynthetic efficiency of the mutant. Genetic analysis of F2 segregation populations derived from the cross of XinonglA and zebra-15 indicated that the zebra leaf trait is controlled by a single recessive nuclear gene. Ninety-eight out of four hundred and eighty pairs of SSR markers showed the diversity between the XinonglA and the zebra-15, their F2 population was then used for gene mapping. Zebra-15 （Z-15） gene was primarily restricted on the short arm of chromosome 5 by 150 F2 recessive individuals, 19.6 cM from marker RM3322 and 6.0 cM from marker RM6082. Thirty-six SSR markers were newly designed in the restricted location, and the Z-15 was finally located between markers nSSR516 and nSSR502 with the physical region 258 kb by using 1,054 F2 recessive individuals.

关键词： molecular mapping rice (Oryza sativa L.) zebra mutant chlorophyll Zebra-15

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基于LMD-Teager变换的功率谱估计

中国激光 2015年第3期42卷 311-317页

作者：蔡剑华王先春湖南文理学院信息研究所湖南常德415000

局域均值分解(LMD)方法是一种新的自适应信号处理方法,Teager能量算子是求解信号瞬时能量的非线性操作算法,能有效提取信号的瞬时能量。结合这两种方法的优点,提出一种基于LMD-Teager变换的功率谱估计方法,给出了算法原理和步骤,讨论了... 详细信息

局域均值分解(LMD)方法是一种新的自适应信号处理方法,Teager能量算子是求解信号瞬时能量的非线性操作算法,能有效提取信号的瞬时能量。结合这两种方法的优点,提出一种基于LMD-Teager变换的功率谱估计方法,给出了算法原理和步骤,讨论了功率谱估计的物理意义,并在与快速傅里叶变换(FFT)方法、小波变换对比的基础上,用短数据序列和非平稳信号进行了仿真验证。结果表明:该方法突破了FFT方法中对所分析的信号必须平稳的要求,更适合于非平稳信号的处理;且对数据长度的要求较傅里叶方法低,而其分析精度和分辨率优于传统的傅氏方法和小波变换。

关键词：傅里叶光学局域均值分解 Teager能量算子功率谱估计时频谱

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研究生教育中导师的自身修养

学位与研究生教育 2020年第12期 1-4页

作者：王贤纯湖南师范大学生命科学学院长沙410081

研究生导师是决定研究生培养质量的关键因素。一名合格的导师应当具备较高的综合素质,其中最重要的素质是师德。不仅要从严规范自己的言行,而且要刻苦钻研,不断提高自己的专业能力,从而给研究生的成长树立学习的榜样,并在研究生培养的... 详细信息

研究生导师是决定研究生培养质量的关键因素。一名合格的导师应当具备较高的综合素质,其中最重要的素质是师德。不仅要从严规范自己的言行,而且要刻苦钻研,不断提高自己的专业能力,从而给研究生的成长树立学习的榜样,并在研究生培养的各个环节给研究生提供有效的指导和帮助。

关键词：研究生导师自身修养师德专业能力

间斑寇蛛毒素的蛋白质化学与蛋白质组学研究新进展

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生物工程学报 2014年第7期30卷 1036-1043页

作者：王贤纯梁宋平湖南师范大学生命科学学院蛋白质化学与发育生物学教育部重点实验室湖南长沙410081

间斑寇蛛Latrodectus tredecimguttatus俗称"黑寡妇"蜘蛛。其毒素不仅存在于毒腺中,而且存在于其身体的其他部分、卵粒甚至新生幼蛛体内。研究间斑寇蛛毒腺和毒腺外材料中的毒素成分,探明它们之间的异同、进化关系和生物学作用,具有重... 详细信息

间斑寇蛛Latrodectus tredecimguttatus俗称"黑寡妇"蜘蛛。其毒素不仅存在于毒腺中,而且存在于其身体的其他部分、卵粒甚至新生幼蛛体内。研究间斑寇蛛毒腺和毒腺外材料中的毒素成分,探明它们之间的异同、进化关系和生物学作用,具有重要的理论和实际意义。现代蛋白质化学和蛋白质组学技术的发展为间斑寇蛛蛋白质和多肽毒素的研究提供了有效手段,从而可以同时从单一纯化蛋白质和组学的层面探究毒素作用的分子基础和作用机制。到目前为止,间斑寇蛛毒素的蛋白质化学与蛋白质组学研究已取得一定的进展,但相关研究尤其是毒腺外材料来源的毒素研究还有待进一步深入。

关键词：毒素蛋白质多肽间斑寇蛛进展

基于组合滤波的鱼油二十碳五烯酸含量近红外光谱检测

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农业工程学报 2016年第1期32卷 312-317页

作者：蔡剑华胡惟文王先春湖南文理学院信息研究所常德415000

为了提高鱼油二十碳五烯酸(eicosapentaenoic acid,EPA)含量的测定精度,该研究将经验模态分解(empirical mode decomposition,EMD)和数学形态学滤波相结合的近红外光谱去噪方法应用于鱼油的一阶导数光谱预处理中,给出了方法的原理和步骤... 详细信息

为了提高鱼油二十碳五烯酸(eicosapentaenoic acid,EPA)含量的测定精度,该研究将经验模态分解(empirical mode decomposition,EMD)和数学形态学滤波相结合的近红外光谱去噪方法应用于鱼油的一阶导数光谱预处理中,给出了方法的原理和步骤,评估了该方法的去噪效果。运用偏最小二乘回归(partial least squares regression,PLSR)建立了鱼油EPA近红外光谱的预测模型,用处理后的光谱计算了鱼油中EPA的含量,并与九点平滑和小波变换方法的处理结果进行了对比分析。结果表明:与传统的九点平滑处理结果相比,信噪比(signal to noise ratio,SNR)从14 d B左右提高到35 d B左右,原始信号与消噪信号之间的标准差由0.005 71降到0.002 26;预测集的决定系数由0.959 3提高到0.987 9,预测均方根误差(root mean square error,RMSE)由0.060 1降为0.031 2。证明了组合的EMD和数学形态学滤波方法在光谱处理过程中的可靠性,提高了鱼油EPA含量近红外光谱的定量分析精度。

关键词：光谱测定模型经验模态分解数学形态滤波近红外光谱鱼油去噪

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基于EMD的土壤有机质含量近红外光谱检测

农业机械学报 2010年第9期41卷 182-186页

作者：蔡剑华王先春胡惟文湖南文理学院信息研究所常德415000

以检测土壤有机质含量为例,探讨经验模态分解在土壤近红外光谱检测中的应用,提出了应用的原理和步骤。用处理后的光谱计算了土壤中的有机质含量,并与九点平滑和小波变换方法的处理结果进行了对比分析。结果表明:与传统的九点平滑处理结... 详细信息

以检测土壤有机质含量为例,探讨经验模态分解在土壤近红外光谱检测中的应用,提出了应用的原理和步骤。用处理后的光谱计算了土壤中的有机质含量,并与九点平滑和小波变换方法的处理结果进行了对比分析。结果表明:与传统的九点平滑处理结果相比,SNR从3 dB左右提高到10 dB左右,原始信号与消噪信号之间的标准差由2.972降到0.901;预测集的决定系数r2由0.941 0提高到0.980 3,预测均方根误差RMSEP由0.670 2降为0.301 1。证明了经验模态分解方法在光谱处理过程中的可靠性,提高了土壤有机质含量近红外光谱的定量分析精度。

关键词：土壤有机质检测经验模态分解近红外光谱信噪比去噪