Evaluation of strategy for analyzing mouse liver plasma membrane proteome

作     者：CHEN Ping ZHANG LiJun LI XuanWen WANG XiE CAO Rui LIU Zhen XIONG JiXian PENG Xia WEI YingJuan YING XingFeng WANG XianChun LIANG SongPing 

作者机构：College of Life SciencesHunan Normal UniversityChangsha 410081China 

出 版 物：《Science China(Life Sciences)》 (中国科学(生命科学英文版))

年 卷 期：2007年第50卷第6期

页      面：731-738页

核心收录：

学科分类：07[理学] 08[工学] 

基　　金：Supported by China National Haman Liver Proteome Project(Grant No.2004 BA711A18) Hunan Provincial Education Department and Hunan Science and Technology Project(Grant Nos.05FJ2002 and 05FJ4018) 

主　　题：liver plasma membrane proteomics mass spectrometry 2DE shotgun 1DE 

摘      要：Plasma membrane(PM)proteome is one of the major subproteomes present in the cell,and is very important in liver *** the present work,C57 mouse liver PM was purified by density-gradient *** purified PM was verified by electron microscope analysis and Western *** results showed that the PM was enriched by more than 20-fold and the contamination of mitochondria was reduced by 2-fold compared with the homogenization *** were separated by 2DE and 1DE,trypsin-digested and submitted to ESI-Q-TOF and MALDI-TOF-TOF mass spectrometry or directly digested in solution and analyzed by LC-ESI ion trap mass *** all,547 non-redundant mouse liver PM proteins were identified,of which 34%contributed to plasma membrane or plasma membrane-related *** study optimized and evaluated the HLPP plasma membrane proteome analysis method and made a systematic analysis on PM proteome.