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微波等离子体刻蚀脱碳预处理对金刚石涂层刀具附着力和切削性能的影响

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机械工程材料 2000年第4期24卷 11-14页

作者：夏琦张志明沈荷生孙方宏何贤昶上海交通大学金属基复合材料国家重点实验室上海200030

用 EACVD法在硬质合金 ( YG6)刀具上沉积金刚石涂层 ;用氢微波等离子体刻蚀的方法对基底进行表面预处理 ,以期提高金刚石涂层的附着力和涂层刀具的切削性能 ;附着力采用压痕法计算测定 ,切削性能通过对碳化硅增强铝基复合材料的切削试... 详细信息

用 EACVD法在硬质合金 ( YG6)刀具上沉积金刚石涂层 ;用氢微波等离子体刻蚀的方法对基底进行表面预处理 ,以期提高金刚石涂层的附着力和涂层刀具的切削性能 ;附着力采用压痕法计算测定 ,切削性能通过对碳化硅增强铝基复合材料的切削试验测定 ;用 X射线应力仪测试了涂层残余应力 ,并就不同预处理对性能的影响进行了分析和讨论。

关键词：微波等离子体刻蚀附着力切削性能

超早熟小麦新品系“花特早”的诱导选育及特征特性分析

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华北农学报 1998年第1期13卷 18-23页

作者：黄冰艳海燕和现昌刘文轩申玉清河南省农业科学院小麦研究所

利用杂种Ｆ１花药培养，选育出了在北方冬麦区罕见的超早熟小麦新品系“花特早”。其突出特点是抽穗早，前期灌浆强度大，比一般小麦早熟７ｄ左右，高抗赤霉病；具有广泛的生态适应性，粒重稳定，丰产性好，为良好的早熟源及赤霉病抗源。

关键词：小麦花药培养早熟性育种花特早

利用CRISPR-Cas9基因编辑技术获得水稻OsYUCCA1基因突变体

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基因组学与应用生物学 2017年第11期36卷 4778-4784页

作者：何先畅黄国强王道洋常淑伟张大兵上海交通大学生命科学技术学院上海200240

为研究植物激素生长素在模式作物水稻中的功能,我们利用CRISPR-Cas9基因编辑技术,设计水稻生长素合成基因Os YUCCA1的两个靶位点,构建Os YUCCA1基因的敲除载体。通过对Os YUCCA1基因序列分析,将合成的靶点序列插入含hsp Cas9n的载体中,... 详细信息

为研究植物激素生长素在模式作物水稻中的功能,我们利用CRISPR-Cas9基因编辑技术,设计水稻生长素合成基因Os YUCCA1的两个靶位点,构建Os YUCCA1基因的敲除载体。通过对Os YUCCA1基因序列分析,将合成的靶点序列插入含hsp Cas9n的载体中,再与p CAMBIA1300重组,构建基因编辑载体,通过农杆菌介导的方法转化水稻品种9522。从78棵转基因苗中鉴定得到针对Os YUCCA1的3种突变类型,包括26棵在外显子第260号碱基处插入碱基A或T两种类型纯合突变和22棵在外显子第447号碱基处插入A、444号碱基C被T替换、445号碱基G被C替换的杂合突变类型。通过分析,发现3种突变株系都存在移码现象而使氨基酸提前终止致使基因突变。本研究成功利用CRISPR-Cas9基因编辑技术敲除了水稻Os YUCCA1基因,为进一步研究Os YUCCA1基因功能提供了理论参考依据。

关键词：水稻 OsYUCCA1 生长素 CRISPR/Cas9 基因编辑技术

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激素及光照对甘薯茎尖分生组织培养的影响

华北农学报 1999年第2期14卷 116-118页

作者：黄冰艳刘文轩刘新涛海燕罗鹏和现昌河南省农业科学院小麦研究所

根据不同基因型甘薯茎尖分生组织在不同激素水平的生长反应，将４个河南主栽甘薯品种分为３类：激素不敏感易成苗型、激素敏感易成苗型和激素不敏感难成苗型。光照时数不是甘薯茎尖培养成苗的限制因素，以不低于１４ｈ／ｄ为好。

关键词：甘薯茎尖分生组织脱毒培养激素光照时数

利用CRISPR-Cas9基因编辑技术获得水稻osarf8-1杂合突变体

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分子植物育种 2018年第12期16卷 3931-3938页

作者：王道洋黄国强陈明姣何先畅常淑伟张大兵上海交通大学生命科学技术学院上海200240

为了研究生长素是如何参与调控植物的生长发育过程,我们利用CRISPR-Cas9基因编辑技术,设计水稻生长素响应因子OsARF8的两个靶点,对水稻中的该基因进行敲除。构建基因编辑载体pBIN-sg R-Cas-Os,通过农杆菌介导转化水稻品种‘9522’。我... 详细信息

为了研究生长素是如何参与调控植物的生长发育过程,我们利用CRISPR-Cas9基因编辑技术,设计水稻生长素响应因子OsARF8的两个靶点,对水稻中的该基因进行敲除。构建基因编辑载体pBIN-sg R-Cas-Os,通过农杆菌介导转化水稻品种‘9522’。我们从潮霉素抗性筛选出的28株转基因植株中鉴定得到针对OsARF8的一种杂合突变类型,共计6棵在外显子第174号碱基处缺失一个C碱基的杂合突变类型osarf8-1,该处碱基突变所对应的是第7个氨基酸的变化,导致亮氨酸(Leu)变成色氨酸(Trp),自此处开始随后的氨基酸序列发生移码突变,并且提前终止于第123个氨基酸,正好位于Os ARF蛋白的DBD功能域的最始端(DBD为120~222个氨基酸),说明DBD功能域在该突变体中完全不能表达,ARF8蛋白的功能将受到极大的影响。本研究成功利用CRISPR-Cas9基因编辑技术精确地敲除了水稻OsARF8基因,为CRISPR技术在水稻中的应用提供了一个可供参考的范例,同时也为进一步研究OsARF8基因功能提供了重要的参考依据。

关键词：水稻 OsARF8 生长素 CRISPR/Cas9

Interference of CsGPA1,theα-submit of G protein,reduces drought tolerance in cucumber seedlings

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Horticultural Plant Journal 2021年第3期7卷 209-220页

作者： Yumei Liu Qinghua Du Longqiang Bai Mintao Sun Yansu Li Chaoxing he Jun Wang xianchang Yu Yan Yan The Institute of Vegetables and Flowers Chinese Academy of Agricultural SciencesBeijing 100081China College of Agricultural and Biological Engineering Heze UniversityHezeShandong 274000China Tianjin Agricultural University Tianjin 300384China School of Horticulture Shanxi Agricultural UniversityTaiguShanxi 030801China

The G proteinα-subunit,GPA1,is an integral component of several signaling pathways in plants,including response to abiotic ***,the molecular mechanism behind these processes remains largely unknown in the cucumber plant(Cucumis sativus L.).In order to further understand the role of CsGPA1 in cucumber under drought stress,changes in plant growth,physiological parameters,and gene expression of CsAQPs were all measured under water stress induced by polyethylene glycol(PEG)using wild type(WT)and CsGPA1-interference(RNAi)cucumber *** results demonstrated that the RNAi plants had lower drought tolerance,displaying seriously withered leaves,lower relative growth rate,lower root-shoot ratio,and lower root activity under drought stress compared to WT *** studies indicated that the suppression of CsGPA1 weakened drought stress tolerance due to higherwater loss rate in the leaves,higher levels of hydrogen peroxide(H2O2),increased malondialdehyde(MDA)content,lower free proline content,lower soluble sugar content,lower soluble protein content,and decreased antioxidant enzyme ***-PCR analysis demonstrated that the interference of CsGPA1 up-regulated the expression of most AQP genes(except for CsPIP2;3 in leaves)and down-regulated the expression of CsPIP1;2,CsPIP1;4,CsPIP2;1,and CsPIP2;4 in roots under drought stress when compared to WT *** results demonstrated that CsGPA1 could function as a positive regulator in drought stress response by decreasing the accumulation of reactive oxygen species(ROS),improving permeable potentials,and reducing water loss in cucumber plants.

关键词： Cucumis sativus CsGPA1 Drought tolerance ROS scavenging Water content

Multifaceted regulatory functions of CsBPC2 in cucumber under salt stress conditions

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Horticulture Research 2023年第5期10卷 126-138页

作者： Shuzhen Li Mintao Sun Li Miao Qinghua Di Lijun Lv xianchang Yu Yan Yan Chaoxing he Jun Wang Aokun Shi Yansu Li State Key Laboratory of Vegetable Biobreeding Institute of Vegetables and FlowersChinese Academy of Agricultural SciencesBeijing 100081China Ganzhou Key Laboratory of Greenhouse Vegetable College of Life ScienceGannan Normal UniversityGanzhou 341000China Collaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province College of Horticulture ScienceZhejiang Agriculture and Forestry UniversityHangzhou 311300ZhejiangChina

BASIC PENTACYSTEINE(BPC)transcription factors are essential regulators of plant growth and ***,BPC functions and the related molecular mechanisms during cucumber(Cucumis sativus L.)responses to abiotic stresses,especially salt stress,remain *** previously determined that salt stress induces CsBPC expression in *** this study,Csbpc2 transgene-free cucumber plants were created using a CRISPR/Cas9-mediated editing system to explore CsBPC functions associated with the salt stress *** Csbpc2 mutants had a hypersensitive phenotype,with increased leaf chlorosis,decreased biomass,and increased malondialdehyde and electrolytic leakage levels under salt stress ***,a mutated CsBPC2 resulted in decreased proline and soluble sugar contents and antioxidant enzyme activities,which led to the accumulation of hydrogen peroxide and superoxide ***,the mutation to CsBPC2 inhibited salinity-induced PM-H+-ATPase and V-H+-ATPase activities,resulting in decreased Na+efflux and increased K+*** findings suggest that CsBPC2 may mediate plant salt stress resistance through its effects on osmoregulation,reactive oxygen species scavenging,and ion homeostasis-related regulatory ***,CsBPC2 also affected ABA *** mutation to CsBPC2 adversely affected salt-induced ABA biosynthesis and the expression of ABA signaling-related *** results indicate that CsBPC2 may enhance the cucumber response to salt *** may also function as an important regulator of ABA biosynthesis and signal *** findings will enrich our understanding of the biological functions of BPCs,especially their roles in abiotic stress responses,thereby providing the theoretical basis for improving crop salt tolerance.

关键词： CRISPR/Cas9 stress functions

Fluoride passivation of ZnO electron transport layers for efcient PbSe colloidal quantum dot photovoltaics

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Frontiers of Optoelectronics 2023年第3期16卷 141-151页

作者： Jungang he You Ge Ya Wang Mohan Yuan Hang Xia Xingchen Zhang Xiao Chen Xia Wang xianchang Zhou Kanghua Li Chao Chen Jiang Tang Hubei Key Laboratory of Plasma Chemistry and Advanced Materials Hubei Engineering Technology Research Center of Optoelectronic and New Energy MaterialsSchool of Materials Science and EngineeringWuhan Institute of TechnologyWuhan 430205China Wuhan National Laboratory for Optoelectronics(WNLO) School of Optical and Electronic InformationSchool of Integrated CircuitsHuazhong University of Science and TechnologyWuhan 430074China

Lead selenide(PbSe)colloidal quantum dots(CQDs)are suitable for the development of the next-generation of photovoltaics(PVs)because of efcient multiple-exciton generation and strong charge coupling *** date,the reported high-efcient PbSe CQD PVs use spin-coated zinc oxide(ZnO)as the electron transport layer(ETL).However,it is found that the surface defects of ZnO present a difculty in completion of passivation,and this impedes the continuous progress of *** address this disadvantage,fuoride(F)anions are employed for the surface passivation of ZnO through a chemical bath deposition method(CBD).The F-passivated ZnO ETL possesses decreased densities of oxygen vacancy and a favorable band *** from these improvements,PbSe CQD PVs report an efciency of 10.04%,comparatively 9.4%higher than that of devices using sol-gel(SG)ZnO as *** are optimistic that this interface passivation strategy has great potential in the development of solution-processed CQD optoelectronic devices.

关键词： Zinc oxide Surface passivation Band alignment Quantum-dot solar cells

Dynamic Expression of mi RNAs and Their Targets in the Response to Drought Stress of Grafted Cucumber Seedlings

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Horticultural Plant Journal 2016年第1期2卷 41-49页

作者： LI Chaohan LI Yansu BAI Longqiang he Chaoxing YU xianchang Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences Horticultural Research Institute Shanghai Academy of Agricultural Sciences

Grafting of cucumber is widely used to improve growth and tolerance to biotic and abiotic stresses. Micro RNAs(miRNAs) regulate plant growth and development, and respond to various stresses through negative, post-transcriptional regulation of the expression of their target genes. We grafted cucumber(Cucumis sativus) scions onto pumpkin(Cucurbita moschata) rootstocks to study the molecular mechanisms of miRNA-mediated graftinginduced responses to drought stress. The relative expressions of 17 selected miRNAs and their predicted target m RNAs were detected by quantitative real-time PCR(q RT-PCR) in leaves and roots of hetero-grafted cucumber seedlings(cucumber as scion and pumpkin as rootstock) and autografted cucumber seedlings(cucumber as scion and rootstock) after 24 hours of 15% PEG6000 treatment. Compared with the expression in leaves of auto-grafted cucumber seedlings, the expressions of most miRNAs in the leaves of hetero-grafted seedlings changed dynamically: induced under normal conditions, and reduced after 3 h of drought stress, and then induced after 8 h and 24 h of drought stress. Similarly, compared with the expression in roots of auto-grafted cucumber seedlings, the expressions of most miRNAs in the roots of hetero-grafted cucumber seedlings changed dynamically: reduced under normal condition, and still reduced after 1 h, but induced after 3 h and 8 h, then reduced significant y after24 h of drought stress. These results are useful for the functional analysis of miRNAs in the mediation of grafting-dependent drought tolerance.

关键词： cucumber graft drought miRNA target