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A Microfluidic System with Active Mixing for Improved Real-Time Isothermal Amplification

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Journal of Beijing Institute of Technology 2022年第3期31卷 275-284页

作者： Dianlong Yang Xiaodan Jiang Yijie Zhou Xiaobin Dong Luyao Liu Lulu Zhang xianbo qiu College of Information Science and Technology Beijing University of Chemical TechnologyBeijing 100029China

To improve the performance of real-time recombinase polymerase amplification(RPA),a microfluidic system with active mixing is developed to optimize the reaction *** of adopting a single typical reaction chamber,a specific reactor including a relatively large chamber in center with two adjacent zig-zag channels at two sides is integrated into the microfluidic *** mixing is achieved by driving the viscous reagent between the chamber and the channel back and forth periodically with an outside compact peristaltic *** avoid reagent evapora-tion,one end of the reactor is sealed with paraffin oil.A hand-held companion device is developed to facilitate real-time RPA amplification within 20 *** whole area of the reactor is heated with a resistance heater to provide uniform reaction *** achieve real-time monitoring,a compact fluorescence detection module is integrated into the hand-held device.A smartphone with custom application software is adopted to control the hand-held device and display the real-time fluorescence *** performances of two cases with and without active on-chip mixing are compared between each other by detecting African swine fever *** has been demonstrated that,with active on-chip mixing,the amplification efficiency and detection sensitivity can be signifi-cantly improved.

关键词： recombinase polymerase amplification(RPA) microfluidic chip active mixing optical detection smartphone

一株长牡蛎致病性盐单胞菌快速检测方法的建立

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农业技术与装备 2024年第5期 115-117,120页

作者：王博谢晓晨邱显博李佳希赵欣妍李晨徐爽大连海洋大学海洋科技与环境学院辽宁大连116023 大连海洋大学水产与生命学院辽宁大连116023

水产动物病原的快速定量检出有助于实现水产病害的提前预警和准确诊断。以分离自北黄海长牡蛎幼虫养殖池塘的一株致病性盐单胞菌7T为研究对象,基于其分子伴侣蛋白Dnak编码基因序列设计特异性引物,能从环境样本中检测出该菌株。通过TA克... 详细信息

水产动物病原的快速定量检出有助于实现水产病害的提前预警和准确诊断。以分离自北黄海长牡蛎幼虫养殖池塘的一株致病性盐单胞菌7T为研究对象,基于其分子伴侣蛋白Dnak编码基因序列设计特异性引物,能从环境样本中检测出该菌株。通过TA克隆构建质粒,利用实时定量PCR方法实现了对该菌株的定量检测。

关键词：长牡蛎盐单胞菌引物实时定量PCR 快速检测

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带前馈补偿的基因扩增反应高精度温控系统

仪器仪表学报 2005年第z1期26卷 831-832,844页

作者：邱宪波袁景淇上海交通大学自动化系上海200030

介绍了基于半导体制冷器的基因扩增反应高精度温度控制系统。系统设计中,针对基因扩增反应热循环过程的自身特点和系统模型的惯性特征,采用了带前馈补偿的复合控制算法来改善控制性能。试验结果表明,包括前馈、PID及Bang-Bang控制在内... 详细信息

介绍了基于半导体制冷器的基因扩增反应高精度温度控制系统。系统设计中,针对基因扩增反应热循环过程的自身特点和系统模型的惯性特征,采用了带前馈补偿的复合控制算法来改善控制性能。试验结果表明,包括前馈、PID及Bang-Bang控制在内的复合控制策略提高了热循环升降温速率和稳态控制精度,性能指标明显优于传统PID控制。

关键词：基因扩增热循环温度控制前馈控制

Feedforward Variable Structural Proportional-Integral-Derivative for Temperature Control of Polymerase Chain Reaction

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Chinese Journal of Chemical Engineering 2006年第2期14卷 200-206页

作者：邱宪波袁景淇汪志锋 Department of Automation Shanghai Jiao Tong University Shanghai 200030 China State Key Laboratory of Bioreactor Engineering East China University of Science and Technology Shanghai 200237 China Department of Automation Shanghai Second Polytech University Shanghai 201209 China

To track the rapidly changing temperature profiles of thermal cycling of polymerase chain reaction （PCR） accurately, an innovative feedforward variable structural proportional-integral-derivative （FVSPID） controller was developed. Based on the step response test data of the heat block, a reduced first order model was estabfished at different operating points. Based on the reduced model, the FVSPID controller combined a feedforward path with the variable structural proportional-integral-derivative （PID） control. The modified feedforward action provided directly the optimal predictive power for the desired setpoint to speed up the dynamic response. To cooperate with the feedforward action, a variable structural PID was applied, where the P mode was used in the case of the largest errors to speed up response, whereas the PD mode was used in the case of larger errors to suppress overshoot, and finally the PID mode was applied for small error conditions to eliminate the steady state offset. Experimental results illustrated that compared to the conventional PID controller, the FVSPID controller can not only reduce the time taken to complete a standard PCR protocol, but also improve the accuracy of gene amplification.

关键词： feedforward variable structural PID controller polymerase chain reaction thermal cycling temperature tracking

Challenges and prospects for room temperature solid-state sodiumsulfur batteries

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Nano Research 2024年第3期17卷 1402-1426页

作者： Yashuang qiu Jing Xu Research Center of Grid Energy Storage and Battery Application School of Electrical and Information EngineeringZhengzhou UniversityZhengzhou 450001China

Room temperature sodium-sulfur(Na-S)batteries,known for their high energy density and low cost,are one of the most promising next-generation energy storage ***,the polysulfide shuttling and uncontrollable Na dendrite growth as well as safety issues caused by the use of organic liquid electrolytes in Na-S cells,have severely hindered their ***-state electrolytes instead of liquid electrolytes are considered to be the most direct and effective solution to solve the above ***,its practical application is still greatly challenged due to the poor interfacial compatibility between the all-solid-state electrolytes and the anode/cathode,ionic conductivity,and the shuttle effect caused by the presence of liquid phase in the quasi-solid-state *** paper presents a comprehensive review of solid-state Na-S batteries from the perspective of regulating interfacial compatibility and improving ionic conductivity as well as suppressing polysulfide *** to different components,solid-state electrolytes were divided into five categories:solid inorganic electrolytes,solid polymer electrolytes,polymer/inorganic solid hybrid electrolytes,gel polymer electrolytes,and liquid–solid inorganic hybrid ***,the prospect of developing high performance solid-state electrolytes to improve the cycling stability of room temperature Na-S cells is envisaged.

关键词： sodium-sulfur batteries solid-state electrolytes ionic conductivity polysulfide shuttling interfacial compatibility

Electrochemical conversion of organic compounds and inorganic small molecules

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Science China Chemistry 2024年第10期67卷 3223-3246页

作者： Weimei Zeng Youai qiu Department of Chemistry Nankai UniversityTianjin 300071China

Electrochemical synthesis presents a facile and sustainable strategy to produce valuable *** review summarizes the most recent advances in electrosynthesizing value-added compounds with inorganic small ***“small molecules”refers to a broad spectrum of chemicals,we mainly focus on the electrochemical conversion of CO_(2),H_(2)O/D_(2)O,NH_(3)and SO_(2)until March *** review intends to compare the results and display general trends throughout the field,with an emphasis on the substrate scope and mechanical ***,we propose several techniques and protocols that can enable a more fundamental understanding of the electrochemical reaction involving inorganic small molecules and help drive this methodology toward commercialization.

关键词： electrochemical synthesis inorganic small molecule organic synthesis carbon dioxide water

Enhanced service stability and thermoelectric performance in Cu_(2)−δSebased composites

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Science China Materials 2024年第7期67卷 2369-2370页

作者： Pengfei qiu Xun Shi State Key Laboratory of High Performance Ceramics and Superfine Microstructure Shanghai Institute of CeramicsChinese Academy of SciencesShanghai 200050China

Thermoelectric(TE)materials can directly convert the industrial waste heat into useful electricity based on the Seebeck effect,providing a promising way to tackle the environmental pollution and global energy crisis[1].In the past several decades,many new high-performance TE materials have been discovered to enrich the TE *** them,Cu_(2)−δSe-based TE materials,which are in line with the“phonon-liquid electron-crystal”concept,have sparked widespread interest due to the high TE figure-of-merit(ZT)and the combination of elements that are nontoxic and earth-abundant[2].In 2012,Liu et al.[3]reported a ZT of 1.5 at 1000 K in the stoichiometric Cu_(2)*** alloying S at the Se-site,the ZT was enhanced to around 2.0 at 1000 K[4].Via incorporating carbon nanotubes,the ZT was further enhanced to 2.4 at 1000 K[5].Moreover,Yang et al.[6]and Zhou et al.[7]reported that the ZT of Cu_(2)Se/BiCuSeO composites can reach 2.7–2.8 around 1000 K.

关键词： composites BiCuSeO performance

y-Tuning: an efficient tuning paradigm for large-scale pre-trained models via label representation learning

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Frontiers of Computer Science 2024年第4期18卷 107-116页

作者： Yitao LIU Chenxin AN Xipeng qiu School of Computer Science Fudan UniversityShanghai 200433China

With current success of large-scale pre-trained models(PTMs),how efficiently adapting PTMs to downstream tasks has attracted tremendous attention,especially for PTMs with billions of *** work focuses on designing parameter-efficient tuning paradigms but needs to save and compute the gradient of the whole computational *** this paper,we propose y-Tuning,an efficient yet effective paradigm to adapt frozen large-scale PTMs to specific downstream tasks.y-Tuning learns dense representations for labels y defined in a given task and aligns them to fixed feature *** computing the gradients of text encoder at training phrase,y-Tuning is not only parameterefficient but also *** results show that for DeBERTaxxL with 1.6 billion parameters,y-Tuning achieves performance more than 96%of full fine-tuning on GLUE Benchmark with only 2%tunable parameters and much fewer training costs.

关键词： pre-trained model lightweight fine-tuning paradigms label representation

The First Five Years of a Phase Theory for Complex Systems and Networks

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IEEE/CAA Journal of Automatica Sinica 2024年第8期11卷 1728-1743页

作者： Dan Wang Wei Chen Li qiu Division of Decision and Control Systems KTH Royal Institute of TechnologyStockholm 10044Sweden Department of Mechanics and Engineering Science&the State Key Laboratory for Turbulence and Complex Systems Peking UniversityBeijing 100871China Hong Kong University of Science and Technology Hong KongChinaand also with the Southern University of Science and TechnologyShenzhen 518055China

In this paper,we review the development of a phase theory for systems and networks in its first five years,represented by a trilogy:Matrix phases and their properties;The MIMO LTI system phase response,its physical interpretations,the small phase theorem,and the sectored real lemma;The synchronization of a multi-agent network using phase *** the end,we also summarize a list of ongoing research on the phase theory and speculate what will happen in the next five years.

关键词： Consensus and synchronization matrix phases multi-agent systems phase theory sectored real lemma small phase theorem.