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Chinese Chemical Letters 2020年第3期31卷 721-724页

作者： Wulin Yang Hao Wang Zirong Pan zhong Li Weiping deng Shanghai Key Laboratory of Chemical Biology School of PharmacyEast China University of Science and TechnologyShanghai 200237China Shanghai Key Laboratory of New Drug Design School of PharmacyEast China University of Science and TechnologyShanghai 200237China Shanghai Collaborative Innovation Center for Biomanufacturing Technology Shanghai 200237China

The chiral phosphoric acid catalyzed asymmetric[3+2]annulation of substituted 2-vinylindoles with azlactones has been established.This reaction represented a practical approach for the synthesis of structurally diverse pyrrolo[1,2-a]indoles with two vicinal stereocenters including one tetrasubstituted stereocenter in good yields and good stereoselectivities under mild conditions.

关键词： [3+2]Annulation Azlactones 2-Vinylindoles Asymmetric organocatalysis Pyrrolo[1,2-a]indoles Chiral phosphoric acid

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井冈山自然保护区木本植物区系分析

福建林业科技 2007年第4期34卷 113-117页

作者：邓贤兰钟金梅曾建忠井冈山学院生命科学学院江西吉安343009

井冈山国家级自然保护区位于江西省南部,木本植物区系丰富,经调查有木本植物991种,隶属于95科305属,其中裸子植物8科13属15种,被子植物87科292属976种;科属组成中以寡种科、寡种属和单种属所占比例较大。植物区系地理成分复杂,科属组成... 详细信息

井冈山国家级自然保护区位于江西省南部,木本植物区系丰富,经调查有木本植物991种,隶属于95科305属,其中裸子植物8科13属15种,被子植物87科292属976种;科属组成中以寡种科、寡种属和单种属所占比例较大。植物区系地理成分复杂,科属组成以热带性成分为主,温带成分占有较大的比例;区系起源古老,保留有大量第四纪冰前期区系成分;国家重点保护植物和特有植物丰富。

关键词：木本植物区系分析井冈山自然保护区

长期给予睾酮致大鼠生精小管伴有生精细胞排列疏松

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中华男科学杂志 2011年第6期17卷 507-510页

作者：邓显忠廖波孔令淑杨正伟川北医学院附属医院泌尿外科四川南充637000 川北医学院形态定量研究室四川南充637000

目的:确定睾酮致大鼠睾丸内睾酮抑制因而精子发生障碍是否伴有生精细胞排列疏松。方法:成年雄性SD大鼠肌注十一酸睾酮[19 mg/(kg.15 d)]130 d后取睾丸组织块,作甲基丙烯酸树脂包埋切片,观察睾丸的组织学变化。结果:除精子形成、精子释... 详细信息

目的:确定睾酮致大鼠睾丸内睾酮抑制因而精子发生障碍是否伴有生精细胞排列疏松。方法:成年雄性SD大鼠肌注十一酸睾酮[19 mg/(kg.15 d)]130 d后取睾丸组织块,作甲基丙烯酸树脂包埋切片,观察睾丸的组织学变化。结果:除精子形成、精子释放障碍等改变以外,11.5%的生精小管轮廓内生精细胞的排列明显较疏松,成串、成束或成团的生精细胞(主要是精母细胞和精子细胞)之间出现朝向小管腔走行的放射状裂隙。结论:生精细胞排列疏松是大鼠睾丸内睾酮抑制所致重要组织学改变之一。

关键词：十一酸睾酮睾丸生精小管生精细胞大鼠

RNA干扰Survivin基因沉默对卵巢癌SKOV3及SKOV3/ADM细胞凋亡的影响

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癌症 2005年第8期24卷 945-950页

作者：邓凯贤钟玲姜梅贤陈维贤陈莹何华重庆医科大学附属二院妇产科重庆400010 四川省中医药研究院附院急诊科四川成都610041

背景与目的:RNA干扰技术被广泛应用于肿瘤基因治疗、抗病毒感染、基因药物筛选等许多领域。Survivin基因在卵巢癌细胞株SKOV3及其耐药株SKOV3/ADM中高表达,是进行卵巢癌基因治疗理想的靶点。本研究探讨Survivin基因沉默后对卵巢癌细胞SK... 详细信息

背景与目的:RNA干扰技术被广泛应用于肿瘤基因治疗、抗病毒感染、基因药物筛选等许多领域。Survivin基因在卵巢癌细胞株SKOV3及其耐药株SKOV3/ADM中高表达,是进行卵巢癌基因治疗理想的靶点。本研究探讨Survivin基因沉默后对卵巢癌细胞SKOV3及其耐药株SKOV3/ADM凋亡的影响。方法:将重组质粒pshRNA鄄Survivin以脂质体转染至SKOV3、SKOV3/ADM细胞。分别用半定量RT鄄PCR、Westernblot检测细胞内SurvivinmRNA和蛋白表达的变化,吖啶橙/溴乙锭(AO/EB)染色法、TUNEL及流式细胞仪检测重组质粒诱导细胞凋亡的情况。结果:转染pshRNA鄄Survivin后,SKOV3、SKOV3/ADM细胞中SurvivinmRNA拷贝数明显减少,抑制率分别为83.79%和91.56%,蛋白质的表达水平显著降低;干扰组细胞凋亡率显著高于对照组(P<0.01)。流式细胞仪检测结果显示,Survivin沉默后48h,SKOV3、SKOV3/ADM的细胞凋亡率分别为14.05%和21.02%,而在对照组未检测到明显凋亡。结论:重组质粒pshRNA鄄Survivin可明显抑制SKOV3、SKOV3/ADM细胞内SurvivinmRNA和蛋白的表达,介导卵巢癌细胞凋亡。

关键词： RNA干扰基因 Survivin 细胞株 SKOV3 卵巢肿瘤凋亡

Effects of synthesis methods on catalytic activities of CoOx–TiO2 for low-temperature NH3-SCR of NO

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Journal of Environmental Sciences 2017年第4期29卷 277-287页

作者： Li Zhu Yiqing Zeng Shule Zhang Jinli deng Qin zhong School of Chemical Engineering Nanjing University of Science and Technology

A series of cobalt doped TiO2（Co-TiO2） and Co Oxloaded TiO2（Co/TiO2） catalysts prepared by sol–gel and impregnation methods respectively were investigated on selective catalytic reduction with NH3（NH3-SCR） of NO. It was found that Co-TiO2 catalyst showed more preferable catalytic activity at low temperature range. From characterization results of XRD,TEM, Raman and FT-IR, Co species were proved to be doped into TiO2 lattice by replaced Ti atoms. After being characterized and analyzed by NH3-TPD, PL, XPS, EPR and DRIFTS, it was found that the better NH3-SCR activities of Co-TiO2 catalysts, compared with Co/TiO2 catalyst, were ascribed to the formation of more oxygen vacancies which further promoted the production of more superoxide ions（O-2）. The superoxide ions were crucial for the formation of low temperature SCR reaction intermediates（NO-3） by reacting with adsorbed NO molecule. Therefore, these aspects were responsible for the higher low temperature NH3-SCR activity of Co-TiO2 catalysts.

关键词： NH3-SCR CoOx–TiO2 catalysts Oxygen vacancies Superoxide ions

稳定干扰AGR2乳腺癌细胞系的建立及其功能

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南昌大学学报（理科版） 2011年第4期35卷 389-392页

作者：邓鸣涛李飞飞吕忠显厦门大学生物医学研究院福建厦门361005

将AGR2基因的shRNA表达序列连接到pSUPER质粒中,获得pSUPER-shAGR2质粒,经测序鉴定后,将pSUPER-shAGR2和pSUPER质粒通过Lipofectamine2000转染MCF7细胞,经流式细胞仪和600μg.mL-1G418筛选获得稳定干扰AGR2的MCF7细胞系和对照细胞系,Western blot检测其RNA干扰效果,MTT检测细胞生长情况;用无血清酚红培养基饥饿12 h后,25 ng.mL-1EGF处理15 min,Western blot检测ERK活化和PARP水平。测序结果证实AGR2shRNA核苷酸链序列插入正确,RNA干扰能有效降低MCF7细胞中AGR2的蛋白表达水平;稳定干扰AGR2的MCF7细胞系,细胞生长缓慢,ERK活化水平显著降低,而凋亡核心蛋白PARP也明显上调。稳定干扰AGR2的MCF7细胞系成功建立,发现RNA干扰AGR2抑制其生长和促进其凋亡,为AGR2作为乳腺癌预后与治疗研究靶点提供更充分的理论依据。

关键词： AGR2 乳腺癌凋亡

The protective effect of Ligustrazin on the Bid and Caspase-3,8,9 of endothelial cells

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The protective effect of Ligustrazin on the Bid and Caspase-...

From trash to treasure: Chemical recycling and upcycling of commodity plastic waste to fuels, high-valued chemicals and advanced materials

中国食品科学技术学会第八届年会暨第六届东西方食品业高层...

作者： xian-feng zhong Ting Luo Ze-yuan deng~* (State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047,China)

＜正＞Objective:The present study was designed to test the hypothesis that Ligustrazin can inhibit hydrogen peroxide（H2O2）-induced injury in human umbilical vein endothelial cells（HUVECs）,and Ligustrazin has a preventive effect on atherosclerosis at the genetic level.Methods:The oxidative injury model was established with H2O2.HUVECs were treated with different concentrations of Ligustrazin in the presence of H2O2.The protective effects of Ligustrazin against H2O2-injury were investigated in the present study.Results:HUVECs incubated with 200?mol/L H2O2 had significantly decreased in cell viability,which was accompanied with apparent cells apoptosis and the up-regulative expression level of Bid,Caspase-3,8,9 mRNA.In addition,the results of quantitative real-time polymerase chain reaction （qRT-PCR）indicated that pretreatment with Ligustrazine down-regulated the expression of Bid and Caspase-3,8,9 mRNA,which had a key role in H2O2-induced cell apoptosis.Conclusion:The study shows that Ligustrazine can protect endothelial cells against oxidative injury induced by H2O2,suggesting that Ligustrazine is a potential compound for the prevention and treatment of atherosclerosis.

关键词： Ligustrazine oxidative damage endothelial cells protective effects qRT-PCR

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Journal of Energy Chemistry 2022年第6期31卷 369-388,I0011页

作者： Fan Zhang Fang Wang xiangyue Wei Yang Yang Shimei Xu Dehui deng Yu-zhong Wang The Collaborative Innovation Center for Eco-Friendly and Fire-Safety Polymeric Materials(MoE) National Engineering Laboratory of Eco-Friendly Polymeric Materials(Sichuan)College of ChemistrySichuan UniversityChengdu 610064SichuanChina College of Polymer Science and Engineering State Key Laboratory of Polymer Materials EngineeringSichuan UniversityChengdu 610064SichuanChina State Key Laboratory of Catalysis Collaborative Innovation Center of Chemistry for Energy MaterialsDalian Institute of Chemical PhysicsChinese Academy of SciencesDalian 116023LiaoningChina

Of all the existing materials, plastics are no doubt among the most versatile ones. However, the extreme increases in plastic production as well as the difficulty of the material for degradation have led to a huge number of plastic wastes. Their recycling rate after disposal is less than 10%, resulting in a series of serious environmental and ecological problems as well as a significant waste of resources. Current recycling methods generally suffer from large energy consumption, the low utilization rate of recycled products with low added value, and produce other waste during the process. Here, we summarized recentlydeveloped chemical recycling ways on commodity plastics, especially new catalytic paths in production of fuels, high-valued chemicals and advanced materials from a single virgin or a mixture of plastic waste,which have emerged as promising ways to valorize waste plastics more economically and environmentally friendly. The new catalyst design criteria as well as innovative catalytic paths and technologies for plastic upcycling are highlighted. Beyond energy recovery by incineration, these approaches demonstrate how waste plastics can be a viable feedstock for energy use with the generation of clean H_(2), high-quality liquid fuels and materials for energy storage, and help inspiring more catalytic process on plastic upcycling to overcome the economical hurdle and building a circular plastic economy.

关键词： Chemical recycling Upcycling Commodity plastic Sustainable development Catalytic path

Comparative Study of 3D-Printed Porous Titanium Alloy with Rod Designs of Three Different Geometric Structures for Orthopaedic Implantation

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Acta Metallurgica Sinica(English Letters) 2024年第1期37卷 54-66页

作者： Jiaxin Li Haozhang zhong Bojun Cao Zhaoyang Ran Jia Tan Liang deng Yongqiang Hao Jinglong Yan Department of Orthopedics The Second Affiliated Hospital of Harbin Medical UniversityHarbin150001China Shanghai Engineering Research Center of Innovative Orthopaedic Instruments and Personalized Medicine Shanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghai200011China Institute of Materials Modification and Modeling Shanghai Jiao Tong UniversityShanghai200240China

Porous titanium alloy is currently widely used in clinical treatment of orthopaedic diseases for its lower elastic modulus and ability to integrate with bone tissue.At the micro-level,cells can respond to different geometries,and at the macro-level,the geometric design of implants will also affect the biological function of cells.In this study,three kinds of porous scaffolds with square,triangular and circle rod shapes were designed and 3D printed.This study observed the proliferation and differentiation of MC3T3-E1 cells during surface culture of the three types of scaffolds.It also evaluated the characteristics of the three scaffolds by means of compression tests and scanning electron microscopy to provide a reference for the design of porous titanium alloy implants for clinical applications.The trends of cell proliferation and gene expression between the three types of scaffolds were observed after treatment with two inhibitors.The results show that the square rod porous scaffolds have the best proliferative and osteogenic activities,and these findings may be due to differences in piezo-type mechanosensitive ion channel component 1(Piezo1)and Yes-associated protein(YAP)expression caused by the macro-geometric topography.

关键词： Porous titanium scaffolds Geometric morphology Cell proliferation Structural design

春季热带大西洋北部海温异常与我国盛夏降水异常的联系

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热带气象学报 2019年第6期35卷 756-766页

作者：李忠贤陈晨曾刚邓伟涛吴玲玲南京信息工程大学气象灾害教育部重点实验室/气候与环境变化国际联合实验室/气象灾害预报预警与评估协同创新中心江苏南京210044 南京信息工程大学大气科学学院江苏南京210044 南京铁道职业技术学院社会科学教学部江苏南京210031

利用Hadley中心的海表温度资料、全国160站降水资料以及NCEP-DOE AMIP-II再分析等资料,运用多种统计分析方法,分析了春季(3—5月)热带大西洋北部海温异常变化特征及其对我国盛夏(7、8月)降水异常的影响。结果表明:春季热带大西洋北部模... 详细信息

利用Hadley中心的海表温度资料、全国160站降水资料以及NCEP-DOE AMIP-II再分析等资料,运用多种统计分析方法,分析了春季(3—5月)热带大西洋北部海温异常变化特征及其对我国盛夏(7、8月)降水异常的影响。结果表明:春季热带大西洋北部模态是热带大西洋海温异常REOF分解的第一模态,方差贡献率为34.5%。热带大西洋北部海温异常年际变率具有明显的季节差异,其中春季最为显著。春季热带大西洋北部海温异常与我国盛夏华中地区降水异常有显著的正相关关系。进一步分析表明,春季热带大西洋北部的海温正异常可以激发出Rossby波,在热带大西洋西北部和热带东太平洋北部产生异常的气旋式环流,引起上述区域的对流层低层(上层)大气出现异常辐合(辐散),并通过热带大西洋北部地区和太平洋之间的垂直环流异常,在中太平洋地区对流层低层大气出现异常辐散,有利于西北太平洋地区产生异常反气旋式环流,异常反气旋西北侧的西南气流有利于水汽输送至我国华中地区,使该地区降水偏多。且这种影响可以通过热带大西洋北部海温异常的持续性,从春季一直持续到盛夏。

关键词：热带大西洋北部海温盛夏降水大气环流