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miR-15b-5p targeting amyloid precursor protein is involved in the anti-amyloid eflect of curcumin in swAPP695-HEK293 cells

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Neural Regeneration Research 2019年第9期14卷 1603-1609页

作者： Hong-Ying liu xian Fu You-Fu li xian-liang li Zhen-Yu Ma Ying Zhang Qing-Chun Gao Department of Neurology the Second Affiliated Hospital of Guangzhou Medical University

Curcumin exerts a neuroprotective effect on Alzheimer’s disease;however,it is not known whether microRNAs are involved in this protective *** study was conducted using swAPP695-HEK293 cells as an Alzheimer’s disease cell ***695-HEK293 cells were treated with 0,0.5,1,2,5,and 10μM curcumin for 24 *** changes in miR-15b-5p,miR-19a-3p,miR-195-5p,miR-101-3p,miR-216b-5p,miR-16-5p and miR-185-5p expression were assessed by real-time quantitative polymerase chain *** mRNA and protein levels of amyloid precursor protein,amyloid-β40 and amyloid-β42 were evaluated by quantitative real-time polymerase chain reaction,western blot assays and enzyme-linked immunosorbent ***695-HEK293 cells were transfected with miR-15b-5p mimic,or treated with 1μM curcumin 24 hours before miR-15b-5p inhibitor *** effects of curcumin on amyloid precursor protein,amyloid-β40 and amyloid-β42 levels were evaluated by western blot assays and enzyme-linked immunosorbent *** assays were used to analyze the interaction between miR-15b-5p and the 3′-untranslated region of amyloid precursor *** results show that amyloid precursor protein and amyloid-βexpression were enhanced in swAPP695-HEK293 cells compared with HEK293 parental *** suppressed the expression of amyloid precursor protein and amyloid-βand up-regulated the expression of miR-15b-5p in swAPP695-HEK293 *** addition,we found a negative association of miR-15b-5p expression with amyloid precursor protein and amyloid-βlevels in the curcumin-treated *** assays revealed that miR-15b-5p impaired the luciferase activity of the plasmid harboring the 3′-untranslated region of amyloid precursor *** findings indicate that curcumin down-regulates the expression of amyloid precursor protein and amyloid-βin swAPP695-HEK293 cells,which was partially mediated by miR-15b-5p via targeting of the 3′-untranslated region of amyloid precursor protein.

关键词： nerve regeneration Alzheimer’s disease natural plant drug curcuminoids miRNAs amyloid precursor protein amyloid-β 3′-untranslated region Luciferase assays neurons neural regeneration

Deposition of crackless freestanding diamond films on Mo substrates with Zr interlayer

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International Journal of Minerals,Metallurgy and Materials 2010年第2期17卷 246-250页

作者： Zheng liu Cheng-ming li liang-xian Chen li-mei Wang li-fu Hei Fan-xiu Lu School of Materials Science and Engineering University of Science and Technology Beijing Beijing 100083 China

The Mo substrate with Zr interlayer,namely composite substrate,was employed to solve the problem of crack formation in the freestanding diamond film *** diamond films deposited on the composite substrates by the direct current arc plasma jet chemical vapor deposition（CVD） method were investigated with scanning electron microscopy（SEM）,X-ray photoelectron spectroscopy （XPS）,X-ray diffraction（XRD）,and Raman *** addition,the stress distribution during the large area freestanding diamond film deposition on the composite substrate was analyzed based on the finite element model *** results reveal that Zr interlayer can be easily destroyed during the post-deposition cooling process,which is helpful for stress release and crack avoiding in diamond films.

关键词： chemical vapor deposition diamond film interlayer thermal stress finite element analysis

Coil Ambient Temperature and Its Influence on the Formation of Blocking Layer in the Electromagnetic Induction-Controlled Automated Steel-Teeming System

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Acta Metallurgica Sinica(English Letters) 2019年第3期32卷 391-400页

作者： Ming He xian-liang li Xing-An liu Xiao-Wei Zhu Tie liu Qiang Wang Key Laboratory of Electromagnetic Processing of Materials(Ministry of Education) Northeastern University School of Metallurgy Northeastern University School of Materials Science and Engineering Northeastern University

Ambient temperature of induction coil is an important factor to influence the implementation of the electromagnetic induction-controlled automated steel-teeming(EICAST) technology. Meanwhile, it also affects the formation of Fe–C alloy blocking layer, which determines the length and installation position of induction coil. An experimental platform was designed to imitate actual working conditions in a ladle with the EICAST system. Ambient temperature of induction coil under high-temperature condition was measured to verify the accuracy of numerical result. After containing molten steel for 120 min and steel teeming for 40 min, the ambient temperature on the upper side of induction coil is 791 °C. In addition, the position of blocking layer in a 110 t ladle was measured by sand-collection and steel-pour methods, and the criterion temperatures of blocking layer in numerical simulation process were corrected. When the refining temperature is1600 °C and the containing time of molten steel is 120 min, the thickness of blocking layer is 130 mm, and the distance between the upper surface of blocking layer and the upper surface of nozzle brick is 154 mm. When the criterion temperatures are 919 °C and 428 °C, the numerical results can be used to confirm the position of blocking layer and the installation position of induction coil.

关键词： Clean steel EICAST technology Heat transfer Blocking layer Coil ambient temperature

Influence Factors Analysis of Fe-C Alloy Blocking Layer in the Electromagnetic Induction-Controlled Automated Steel Teeming Technology

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Acta Metallurgica Sinica(English Letters) 2020年第5期33卷 671-678页

作者： Ming He xian-liang li Qing-Wei Wang Qiang Wang Zhi-Yuan liu Chong-Jun Wang Key Laboratory of Electromagnetic Processing of Materials(Ministry of Education) Northeastern UniversityShenyang 110819China School of Metallurgy Northeastern UniversityShenyang 110819China School of Materials Science and Engineering Northeastern UniversityShenyang 110819China Tangshan Heavy Plate Company limited Tangshan 063610China

In the electromagnetic induction-controlled automated steel teeming(EICAST)technology of ladle,the height and location of the blocking layer are critical factors to determine the structure size and installation location of induction ***,they are also the key parameters affecting the successful implementation of this new *** this paper,the influence of the liquid steel temperature,the holding time and the alloy composition on the height and location of the blocking layer were studied by numerical *** simulation results were verified by 40 t ladle industrial ***,the regulation approach of the blocking layer was determined,and the determination process of coil size and its installation location were also *** results show that the location of the blocking layer moves down with the increase in the liquid steel temperature and the holding *** height of the blocking layer decreases with the increase in the liquid steel temperature;however,it increases with the increase in the holding *** height and location of the blocking layer can be largely adjusted by changing the alloy composition of filling particles in the upper *** the liquid steel temperature is 1550℃,the holding time is 180 min and the alloy composition is confirmed,the melting layer height is 120 mm,and the blocking layer height is 129 mm,which are beneficial to design and installation of induction *** results are very important for the industrial implementation of the EICAST technology.

关键词： Electromagnetic induction-controlled automated steel teeming(EICAST) Blocking layer liquid steel temperature Holding time Alloy composition

Predictors of incidental gallbladder cancer in elderly patients

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Hepatobiliary & Pancreatic Diseases International 2015年第1期14卷 96-100页

作者： Ji-Qiao Zhu Dong-Dong Han xian-liang li Jian-Tao Kou Hua Fan Qiang He Department of Hepatobiliary Surgery Beijing Chaoyang Hospital Capital Medical University

BACKGROUND： At the time of diagnosis, most patients with gallbladder cancer are in advanced stage and the cancer is unresectable. Long-term survivors are usually seen in a small number of patients with incidental gallbladder cancer. This study aimed to identify preoperative predictors of incidental gallbladder cancer in elderly ***： A total of 4014 patients of more than 44 years old who had undergone cholecystectomy at our department from January 2000 to December 2010 were retrospectively reviewed Univariate and multivariate modalities were used to identify the predictive factors of incidental gallbladder ***： Twenty-nine of the 4014 patients who had under gone cholecystectomy for benign gallbladder diseases were histologically diagnosed as having incidental gallbladder can cer. Multivariate analysis identified that elevated carbohydrate antigen 19-9 combined with carcinoembryonic antigen and/or carbohydrate antigen 125 （P=0.045）, a gallbladder polyp greater than or equal to 1.2 cm （P=0.043） and focal gallblad der wall thickening of more than or equal to 5 mm （P=0.002）were predictive factors of incidental gallbladder ***： Cholecystectomy is suggested for patients with these predictive factors and intraoperative frozen section should be considered to rule out carcinoma.

关键词： incidental gallbladder cancer predictive factors resection

Deceased donor liver transplantation for Budd–Chiari syndrome:Long-segmental thrombosis of the inferior vena cava with extensive collateral circulation

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Hepatobiliary & Pancreatic Diseases International 2020年第3期19卷 288-290页

作者： Zi-Xi liu Ji-Qiao Zhu Jun Ma Jian-Tao Kou xian-liang li Qiang He Department of Hepatobiliary and Pancreaticosplenic Surgery Beijing Chaoyang HospitalCapital Medical UniversityNo.8 Gongtinan RoadChaoyang DistrictBeijing 100020China

Budd–Chiari syndrome(BCS)is an infrequent clinical disease resulting from obstruction of the hepatic venous outflow tract anywhere from small hepatic veins to the inferior vena cava(IVC)[1].Currently,step-wise treatment strategy has been proposed and widely adopted based on the patient's status,which contains anticoagulation,thrombolysis,percutaneous recanalization,transjugular intrahepatic portosystemic shunt(TIPS)and surgical shunt[2].

关键词： Chiari hepatic vena

Bilioenteric anastomotic stricture in patients with benign and malignant tumors: prevalence,risk factors and treatment

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Hepatobiliary & Pancreatic Diseases International 2017年第4期16卷 412-417页

作者： Ji-Qiao Zhu xian-liang li Jian-Tao Kou Hong-Meng Dong Huan-Ye liu Chun Bai Jun Ma Qiang He Department of Hepatobiliary and Pancreaticosplenic Surgery Beijing Chaoyang HospitalCapital Medical University

BACKGROUND: Stricture formation at the bilioenteric anastomosis is a rare but important postoperative complication. However, information on this complication is lacking in the literature. In the present study, we aimed to assess its prevalence and predictive factors, and report our experience in managing bilioenteric anastomotic strictures over a ten-year period. METHODS: A total of 420 patients who had undergone bilioenteric anastomosis due to benign or malignant tumors between February 2001 and December 2011 were retrospectively reviewed. Univariate and multivariate modalities were used to identify predictive factors for anastomotic stricture occurrence. Furthermore, the treatment of anastomotic stricture was analyzed. RESULTS: Twenty-one patients (5.0%) were diagnosed with bilioenteric anastomotic stricture. There were 12 males and 9 females with a mean age of 61.6 years. The median time after operation to anastomotic stricture was 13.6 months (range, 1 month to 5 years). Multivariate analysis identified that surgeon volume (6 mm) (odds ratio: 2.871; P=0.0002) had a negative association. Balloon dilation was performed in 18 patients, biliary stenting in 6 patients, and reoperation in 4 patients. Five patients died of tumor recurrence, and one of heart disease. CONCLUSIONS: Bilioenteric anastomotic stricture is an uncommon complication that can be treated primarily by interventional procedures. Bilioenteric anastomosis may be performed by a surgeon in his earlier training period under the guidance of an experienced surgeon. Bile duct size >6 mm may play a protective role.

关键词： anastomotic stricture bilioenteric anastomosis bile duct size surgeon volume

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基于人工蜂群优化的多段支持度数据挖掘仿真

计算机仿真 2019年第7期36卷 273-276页

作者：梁显丽内蒙古农业大学

针对当前多段支持度数据挖掘方法中存在的挖掘效率较低,查全率较低等不足之处,通过相关算法研究,提出基于人工蜂群优化的多段支持度数据挖掘方法。以数据项集支持度思想与重要性质为依据,计算数据项集的综合支持度;构建数据关联规则支... 详细信息

针对当前多段支持度数据挖掘方法中存在的挖掘效率较低,查全率较低等不足之处,通过相关算法研究,提出基于人工蜂群优化的多段支持度数据挖掘方法。以数据项集支持度思想与重要性质为依据,计算数据项集的综合支持度;构建数据关联规则支持度函数,结合最大值最小值定理及介质定理,确定关联规则支持度;根据数据综合支持度及关联规则支持度计算结果,利用支持向量机进行数据空间映射以及数据分类;通过构建精英蜂群策略及侦查蜂搜索机制更新,对传统人工蜂群算法进行改进,利用改进后人工蜂群算法优化支持向量机参数,提高数据分类挖掘精度。实验结果表明,所提方法的挖掘效率及查全率高于实验对比方法,且支持度预估误差明显低于实验对比方法。

关键词：人工蜂群优化多段支持度关联规则数据挖掘

Risk factors of severe ischemic biliary complications after liver transplantation

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Hepatobiliary & Pancreatic Diseases International 2011年第4期10卷 374-379页

作者： Ming-Feng Wang,Zhong-Kui Jin,Da-Zhi Chen,xian-liang li,Xin Zhao and Hua Fan Department of Hepatobiliary and Pancreaticosplenic Surgery,Beijing Chaoyang Hospital,Capital Medical University,Beijing 100020,China Department of Hepatobiliary and Pancreaticosplenic Surgery Beijing Chaoyang Hospital Capital Medical University Beijing 100020 China

BACKGROUND:Ischemia-related biliary tract complications remain high after orthotopic liver *** ischemic biliary complications often involve the hepatic duct bifurcation and left hepatic duct,resulting finally in obstructive *** and management of such complications remain a challenge for transplant ***:All 160 patients were followed up for at least 180 days after ***-way analysis of variance (ANOVA) and comparative univariate analysis were made using 3 groups (no complications;mild complications;severe complications),to analyze risk factors associated with biliary *** logistic regression and linear regression analysis were used to analyze independent risk factors for severe ischemic biliary complications,after excluding other confounding ***:By ANOVA and comparative univariate analysis,the risk factors associated with biliary complications were preoperative bilirubin level (P=0.007) and T-tube stenting of the anastomosis (P=0.016).Multiple logistic regression analysis showed that the use of T-tube and preoperative serum bilirubin were not independent risk factors for severe ischemic biliary complications after orthotopic liver ***-square analysis indicated that in the incidence of severe ischemic biliary lesions,bile duct second warm ischemic time longer than 60 minutes was a significant risk *** regression demonstrated a negative correlation between cold preservation time and warm ischemia ***:Preoperative serum bilirubin level and the use of T-tube stenting of the anastomosis were independent risk factors for biliary complications after liver transplantation,but not for severe ischemic biliary *** second warm ischemia time of bile duct longer than 60minutes and prolonged bile duct second warm ischemia time combined with cold preservation time were significant risk factors for severe ischemic biliary complications after

关键词： liver transplantation ischemic biliary complications warm ischemia cold preservation