检索结果-南通市图书馆

在线全文

学校读者我要写书评

暂无评论

樗蚕丝腺5SrRNA的核苷酸顺序

生物化学杂志 1988年第6期 502-507页

作者：周先锦易文仲黄君霆辜祥荣祁国荣中国农业科学院蚕业研究所中国科学院上海生物化学研究所中国科学院上海生物化学研究所江苏、镇江

本文用凝胶直读法、末端鉴定法等相配合,测定了樗蚕(Philosamia cynthia)絲腺5SrRNA的核苷酸顺序:AGACAACGUCCAUACCACGUUGAAAACACCGGUUCUCGUCCGAUCACCGAAGUCAAGCAACGUCGGGCGCGGUCAGUACUUGGAUGGGUGACCGCCUGGGAACACCGCGUGCUGUUGGCUU比较... 详细信息

关键词：樗蚕 5S rRNA RNA序列分析二级结构自由能序列同源性

Endothelial precursor cells promote angiogenesis in hepatocellular carcinoma

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2012年第35期18卷 4925-4933页

作者： Xi-Tai Sun xian-Wen Yuan Hai-Tao Zhu Zheng-Ming Deng De-Cai Yu xiang zhou Yi-Tao Ding Department of Hepatobiliary Surgery the Affiliated Drum Tower Hospital of Nanjing University Medi-cal School Nanjing 210008 Jiangsu Province China Institute of Hepatobiliary Surgery Nanjing University Nanjing 210008 Jiangsu Province China NanjingUniversity Nanjing 210008 Jiangsu Province China Department of Pathology the Affiliated Drum Tow-er Hospital School of Medicine Nanjing University Nanjing210008 Jiangsu Province China

AIM:To investigate the role of bone marrow-derived endothelial progenitor cells(EPCs) in the angiogenesis of hepatocellular carcinoma(HCC).METHODS:The bone marrow of HCC mice was reconstructed by transplanting green fluorescent protein(GFP) + bone marrow *** concentration of circulating EPCs was determined by colony-forming assays and fluorescence-activated cell *** and tissue levels of vascular endothelial growth factor(VEGF) and colony-stimulating factor(CSF) were quantified by enzyme-linked immunosorbent *** distribution of EPCs in tumor and tumor-free tissues was detected by immunohistochemistry and real-time polymerase chain *** incorporation of EPCs into hepatic vessels was examined by immunofluorescence and *** proportion of EPCs in vessels was then ***:The HCC model was successful *** flow cytometry analysis showed the mean percentage of CD133CD34 and CD133VEGFR2 double positive cells in HCC mice was 0.45% ± 0.16% and 0.20% ± 0.09% *** values are much higher than in the sham-operation group(0.11% ± 0.13%,0.05% ± 0.11%,n = 9) at 14 d after *** 21 d,the mean percentage of circulating CD133CD34 and CD133VEGFR2 cells is 0.23% ± 0.19%,0.25% ± 0.15% in HCC model vs 0.05% ± 0.04%,0.12% ± 0.11% in *** to the transient increase observed in controls,the higher level of circulating EPCs were induced by *** addition,the level of serum VEGF and CSF increased gradually in HCC,reaching its peak 14 d after modeling,then slowly *** sections stained for the CD133 and CD34 antigens showed that the CD133+ and CD34+ VEGFR2 cells were mostly recruited to HCC tissue and concentrated in tumor *** fluorescence microscopy,the bone-marrow(BM)-derived cells labeled with GFP were concentrated in the same *** relative levels of CD133 and CD34 gene expression were elevated in tumors,around 5.0 and 3.8 times that of the tumor free area.

关键词：肿瘤血管生成肝细胞肝癌内皮祖细胞前体细胞血管内皮生长因子血管内皮细胞绿色荧光蛋白免疫组化染色

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

农村教师获得感的现状与对策研究

特立研究 2020年第4期 49-53页

作者：刘艳霞周险峰湖南科技大学教育学院湖南湘潭411201

农村教师是教育均衡发展和乡村振兴的重要力量和关键因素,而获得感是促进教师发展的动力源泉,获得感的提升有利于农村教师安心从教、扎根基层。调查发现,影响农村教师获得感的主要困境包括收入待遇低、身份认同低、归属感弱、受尊重感... 详细信息

农村教师是教育均衡发展和乡村振兴的重要力量和关键因素,而获得感是促进教师发展的动力源泉,获得感的提升有利于农村教师安心从教、扎根基层。调查发现,影响农村教师获得感的主要困境包括收入待遇低、身份认同低、归属感弱、受尊重感钝化、职业发展受阻等。为此,应提高待遇、提升教师的物质满足感,完善农村教师荣誉制度、增强教师身份认同感,畅通职业发展渠道、提高教师自我成就感,实现农村教师真正“下得去、留得住、教得好、走得远”,推进农村基础教育的发展,促进教育公平问题的解决。

关键词：农村教师中小学教师教师获得感

上海市浦东新区脑梗死患者复发及影响因素分析

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

上海预防医学 2016年第6期28卷 358-360,375页

作者：于思雨周先锋阮晓楠芮欣忆邱桦周弋吴抗王小楠毕文婕肖林海郝莉鹏上海市浦东新区疾病预防控制中心上海200136

目的分析上海市浦东新区脑梗死患者复发情况及影响因素,探讨社区控制脑梗死复发的有效干预措施。方法在浦东新区监测点社区报病网络中,对辖区居民发生的脑梗死事件相关信息进行收集、登记及核实,以2012年新发脑梗死患者628例为队列,观... 详细信息

目的分析上海市浦东新区脑梗死患者复发情况及影响因素,探讨社区控制脑梗死复发的有效干预措施。方法在浦东新区监测点社区报病网络中,对辖区居民发生的脑梗死事件相关信息进行收集、登记及核实,以2012年新发脑梗死患者628例为队列,观察截至2014年年末时该人群的复发情况并分析复发的影响因素。结果脑梗死患者的再次复发率为9.08%。高血压(OR=4.797,P=0.001)、长期吸烟(OR=2.844,P=0.005)、腹部肥胖或超重(OR=2.421,P=0.041)和务农(OR=3.723,P<0.001)是脑梗死复发的独立危险因素。结论浦东新区居民脑梗死复发受多方面因素影响。

关键词：脑梗死复发影响因素

上海口岸输入性传染性肺结核病患者中结核分枝杆菌北京型特征及耐药性分析

在线全文

学校读者我要写书评

暂无评论

微生物与感染 2015年第1期10卷 34-39页

作者：孟成艳方筠王健沈遥杰叶魏章琪陆晔张晓航周娴上海国际旅行卫生保健中心上海200335 复旦大学附属华山医院感染科上海200040

本研究旨在了解上海口岸输入性传染性肺结核病患者中结核分枝杆菌北京型特征及其对一线抗结核药物的耐药情况，从而正确评估输入性结核病给上海地区带来的公共卫生危害，同时为地区传染性结核病防治策略的制定提供依据。针对2009年1月-2... 详细信息

本研究旨在了解上海口岸输入性传染性肺结核病患者中结核分枝杆菌北京型特征及其对一线抗结核药物的耐药情况，从而正确评估输入性结核病给上海地区带来的公共卫生危害，同时为地区传染性结核病防治策略的制定提供依据。针对2009年1月-2013年5月上海口岸入境体检人员中胸部影像学诊断疑似活动性肺结核病的人群，采集其连续3d的晨痰分离培养结核分枝杆菌，用MGIT960培养法分析其对抗结核药物（链霉素、异烟肼、利福平、乙胺丁醇、吡嗪酰胺）的耐药情况，用目标缺失多重聚合酶链反应（***）进行结核分枝杆菌北京型分子分型，同时采集其人口学资料。期间共监测到入境外籍疑似活动性肺结核病者193例，其中50例痰液中分离培养到结核分枝杆菌，菌培阳率为25．9％，与我国结核病普查工作中活动性肺结核病菌培阳率（25．9％）相同。分离培养并成功传代、分型的40株结核分枝杆菌中，北京型占57．5％（23／40），显著低于同期上海口岸出境人群中的90．2％（37／41）。输入性结核分枝杆菌北京型主要来自东南亚地区（71．4％，5／7）和西太平洋地区（57．1％，16／28）。一线5种抗结核药物的耐药性检测结果显示，输入性结核分枝杆菌北京型的总耐药率为30．4％（7／23），接近上海口岸出境人群的34．1％。输入性耐多药菌株占2．4％（1／42），分子分型结果显示为非北京型。输入性传染性肺结核病对吡嗪酰胺的耐药率为16．7％，显著高于上海口岸出境人群的2．4％。结果提示，上海口岸输入性肺结核病患者中结核分枝杆菌北京型所占比例显著低于同期出境人群，未发现与性别和年龄相关，未发现输入性结核分枝杆菌北京型对抗结核药物耐药性的显著变化。输入性耐药性结核病带来的公共卫生危害不容忽视，在直接督导下的短程化疗（DOTS）方案中需考虑其高吡嗪酰胺耐药特征。在口岸公共卫生安全风险评估中，需重视输入性结核分枝杆菌北京型与本地流行株的差异。

关键词：结核病结核分枝杆菌复合群北京型公共卫生安全风险评估

Effect of chitooligosaccharides on cyclin D1,bcl-xl and bcl-2 mRNA expression in A549 cells using quantitative PCR

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2011年第15期56卷 1629-1632页

作者： LI xian WANG Ju CHEN XiaoJia TIAN JinHuan LI LiHua ZHAO MingYan JIAO YanPeng zhou ChangRen Department of Materials Science and Engineering Jinan University Guangzhou 510632 China1 Bioengineering Institute Jinan University Guangzhou 510632 China

Chitooligosaccharide (COS) is derived from the chemical and enzymatic hydrolysis of chitosan and has been reported to have potent antitumor *** study investigated the effects of five chitooligomers ranging from the dimer form to the hexamer form (chitobiose,chitotriose,chitotetraose,chitopentaose,chitohexaose) on the expression of cyclin D1,bcl-2 and bcl-xl mRNA in A549 cells using reverse transcription quantitative real-time *** demonstrated that,of the five chitooligomers used,chitohexaose (COS6) had the most potent inhibitory effect on A549 cell ***6 also significantly down regulated cyclin D1 and bcl-xl mRNA expression *** data suggested that COS6 exerts its antitumor activity by two different mechanisms:(1) COS6-mediated inhibition of Cyclin D1 levels leads to suppression of tumor cell proliferation;and (2) COS6-mediated down-regulation of the pro-survival protein,Bcl-xl,promotes the apoptosis of tumor cells.

关键词：细胞周期蛋白D1 mRNA表达水平 A549细胞壳寡糖定量PCR 肿瘤细胞凋亡抗肿瘤活性 cyclin

Two-photon absorption and frequency-upconversion properties of a new organic dye HMASPS

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2001年第14期46卷 1220-1223页

作者： Guangyong zhou Yan Ren Chun Wang Dong Wang xian Zhao Qi Fang Zongshu Shao Minhua Jiang Shandong Univ Inst Crystal Mat State Key Lab Crystal Mat Jinan 250100 Peoples R China

Two-photon absorption (TPA) and frequency-upconversion properties of a new upconversion laser dye trans-4-[p-(N-hydroxyethyl-N-methyl-amino)styryl]-N-meth-ylpyridinium toluene-p-sulfonate (abbreviated to HMASPS) were reported in this note. The linear absorption, TPA, single-photon induced fluorescence, TPA induced fluorescence and TPA induced upconverted lasing spectra of HMASPS solution in dimethyl formamide (abbreviated to DMF) were measured at room temperature. The red shift for the central wavelength of TPA induced fluorescence peak, which was compared with that of the single-photon induced fluorescence peak, and the blue shift for that of TPA induced up-converted lasing compared with that of TPA induced fluorescence, were explained by using re-absorption effect. TPA peak was at 930 nm. There is an 11 nm blue shift for two-photon energy of TPA peak compared with the linear absorption peak. The molecular TPA cross-section σ’2 at1064 nm was measured to be 6.0×10-48 cm4 .s/photon by using the open

关键词： organic dye HMASPS two-photon absorption fre- quency-upconverted lasing.

在线全文

学校读者我要写书评

暂无评论

供电公司直供契约机制设计

水电能源科学 2004年第2期22卷 64-67页

作者：梁劲王先甲周静王平周策武汉大学系统工程研究所湖北武汉430072 武汉大学电气工程学院湖北武汉430072 咸宁市供电局湖北咸宁437000 国家电网公司北京100031

通过对供电公司与大用户之间供电契约机制的研究,建立了供电公司与大用户在垄断和竞争市场结构下的供电契约设计模型:在获取的工业大用户消费偏好的信息不完全的情况下,同时考虑到外部竞争的影响,供电公司为了索取不同类型消费者的剩余... 详细信息

通过对供电公司与大用户之间供电契约机制的研究,建立了供电公司与大用户在垄断和竞争市场结构下的供电契约设计模型:在获取的工业大用户消费偏好的信息不完全的情况下,同时考虑到外部竞争的影响,供电公司为了索取不同类型消费者的剩余以及保证其与自身签约,必须针对不同类型消费者来设计不同的售电契约。对大用户直供契约机制作了一些尝试性的研究。

关键词：电力市场供电契约机制设计信息租金

Mapping Heterotic Loci for Yield and Agronomic Traits Using Chromosome Segment Introgression Lines in Cotton

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2013年第8期55卷 759-774页

作者： xian Guo Yuping Guo Jun Ma Fang Wang Mizhen Sun Lijuan Gui Jiajia zhou xianliang Song Xuezhen Sun Tianzhen Zhang College of Agronomy/National Key Laboratory of Crop Biology Shandong Agricultural University National Key Laboratory of Crop Genetics and Germplasm Enhancement Cotton Research InstituteNanjing Agricultural University

In the present study, a set of chromosome segment introgression lines （CSILs） using Gossypium hirsutum L. TM-1 as the recipient parent and G. barbadense Hai7124 as the donor parent were used to explore the genetic basis of heterosis for interspecific hybrids. Two sets of F1 populations individually derived from CSILs crossing with both parents were configured to investigate heterotic loci （HL） and substitution effect loci （SL）. A total of 58 HL and 39 SL were identified in 3 years. One stable HL, hLP-A4-3, could be detected in all 3 years. Three HLs, hBS-A8-1, hLP-D6-1, and hSI-D7-11, could be detected in 2 years. Four SLs, sBSoD7- 1, sLP-A8-1, sLP-D7-1, and sLP-D12-1, could be detected in 2 years. HL and SL tended to be distributed in some HL-rich chromosome segments with close positions. Compared with QTL detected in a former study, HL showed little overlap with QTL, indicating that trait phenotype and heterosis might be controlled by different sets of loci. All three forms of genetic effects （partial-, full-, over-dominant） were identified, while the over-dominant effect made the main contribution to heterosis. These results may help lay the foundation for clarifying the heredity mechanism of heterosis in cotton.

关键词： Cotton chromosome segment introgression lines heterosis heterotic loci.