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Outcomes of simple saline-coupled bipolar electrocautery for hepatic resection

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World Journal of Gastroenterology 2014年第26期20卷 8638-8645页

作者： Jian-Yang Guo De-Wei Li Rui Liao Ping Huang xian-Bing kong Ji-Ming Wang Hong-Lin Wang Shi-Qiao Luo Xiong Yan Cheng-You Du Department of Hepatobiliary Surgery The First Affiliated HospitalChongqing Medical University

AIM: To evaluate the application of bipolar coagulation (BIP) in hepatectomy by comparing the efficacy of BIP alone, cavitron ultrasonic surgical aspirator (CUSA) + BIP and conventional clamp crushing (CLAMP).

关键词： Hepatectomy Surgical procedures Blood loss Complications Hospital stay Comparative study

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基于OKID的损耗因子辨识方法研究

振动工程学报 2011年第3期24卷 253-259页

作者：孔宪仁张红亮杨正贤哈尔滨工业大学卫星技术研究所黑龙江哈尔滨150001

提出了利用观测器/卡尔曼滤波辨识(OK ID)方法辨识系统的最小阶功率流模型。首先直接由测量的输入功率和能量的时域数据辨识功率流系统的M arkov参数,然后利用特征系统实现算法得到功率流模型的最小实现。同时对统计能量分析模型修正方... 详细信息

提出了利用观测器/卡尔曼滤波辨识(OK ID)方法辨识系统的最小阶功率流模型。首先直接由测量的输入功率和能量的时域数据辨识功率流系统的M arkov参数,然后利用特征系统实现算法得到功率流模型的最小实现。同时对统计能量分析模型修正方法进行了改进,将初始模型子系统间的耦合信息作为附加约束条件,并根据约束最优化思想寻求初始损耗因子相对误差矩阵的最小范数解,进一步可得到辨识的损耗因子参数。通过5子系统结构的实例仿真分析,验证了OK ID功率流模型辨识方法可行并具有很好的抗噪性能;与传统SEA模型修正算法相比,改进方法对初始SEA模型有更好的适应性,修正参数更好地保持了物理意义。研究的辨识方法可有效提高内损耗因子和耦合损耗因子的实验辨识精度,扩展实验SEA参数识别的工程应用范围。

关键词：统计能量分析 OKID 特征系统实现算法内损耗因子耦合损耗因子

Model Updating for Solar Array Based on the Improved GA-PSO Hybrid Algorithm

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Model Updating for Solar Array Based on the Improved GA-PSO ...

促进网络融合，服务经济社会发展——中国通信学会第六届学术年会

作者： kong xian-ren QIN Yu-ling LUO Wen-bo Research Center of Satellite Technology Harbin Institute of Technology China Academy of Space Technology

The solar array whose quality has great influence on the work efficiency of the spacecraft is the most important power supply system and the honeycomb sandwich panel which has high specific strength and stiffness is the perfect material for solar *** finite element model(FEM) of the honeycomb sandwich panel includes idealized hypothesis and simplification,which results in the difference between the calculating data and the test *** this paper the honeycomb sandwich panel is equivalent to a structure which can be calculated by NASTRAN based on the equivalence theory and then the improved Genetic- Partical Swarm Optimization(GA-FSO) algorithm is used to update the FEM,the updated precision and efficiency of the GA,PSO and GA- PSO are compared and the results show that these random search algorithms can update high order modes even use the incomplete low order *** improved GA-PSO algorithm can use GA to efficiently search for the global optimal solution at the early stage,and use PSO to intensively search for the local optimal solution at the later stage,it has higher searching efficiency and precision,the updated model has good approaching and prediction ability and can be taken as the fiducial model for the following calculation.

关键词： spacecraft finite element model model updating solar array GA-PSO algorithm global-optimal solution

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多粗糙峰弹塑性接触的有限元分析

摩擦学学报 2000年第3期20卷 202-206页

作者：杨楠陈大融孔宪梅清华大学摩擦学国家重点实验室北京100084

采用具有一定数目圆形粗糙峰的刚性表面对弹塑性半无限体进行压下的模型来模拟多粗糙峰接触 ,并用有限元法对该模型进行了弹塑性分析 ,揭示了多粗糙峰对于接触区应力状态的影响规律 .发现中心接触区的变形主要受到一定数目邻近粗糙峰的... 详细信息

采用具有一定数目圆形粗糙峰的刚性表面对弹塑性半无限体进行压下的模型来模拟多粗糙峰接触 ,并用有限元法对该模型进行了弹塑性分析 ,揭示了多粗糙峰对于接触区应力状态的影响规律 .发现中心接触区的变形主要受到一定数目邻近粗糙峰的影响 ,而处于较远处的其它粗糙峰的影响较小 .同弹性接触相比 ,在弹塑性接触过程中有更多的邻近粗糙峰对中心接触区发生作用 ,改变粗糙峰的间距。

关键词：多粗糙峰弹塑性接触有限元分析

The expression and antigenicity identification of recombinant rat TGF-β1 in bacteria

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Cell Research 2001年第2期11卷 95-100页

作者： GAO CHUN FANG, xian TAO kong, AXEL M GRESSNER, RALF WEISKIRCHEN Department of Laboratory Medicine, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China Institute of Clinical Chemistry and Pathobiochemistry, Central Laboratory, R 1Department of Laboratory Medicine Changzheng Hospital Second Military Medical University Shanghai 200003 China 1Department of Laboratory Medicine Changzheng Hospital Second Military Medical University Shanghai 200003 China 2Institute of Clinical Chemistry and Pathobiochemistry Central Laboratory RWTH-University Hospital Pauwelsstr 30 D-52074 Aachen Germany 2Institute of Clinical Chemistry and Pathobiochemistry Central Laboratory RWTH-University Hospital Pauwelsstr 30 D-52074 Aachen Germany

In order to study structure-function details of TGF-β1, the recombinant mature form of rat TGF-β1 was expressed in bacteria. Synthesis of the 112 amino-acid carboxyl-terminal part of TGF-β1 (amino acid 279-390) was controlled by an inducible gene expression system based on bacteriophage T7 RNA polymerase. This system allowed an active and selective synthesis of recombinant TGF-β1. The molecular weight of expressed TGF-β1 monomer determined on SDS-polyacrylamide gel under reducing conditions was about 13 kD. Serial detergent washes combined with a single gel-filtration purification step were sufficient to purify the expression product to homogeneity. Amino-terminal sequencing revealed that the N-terminal of the recombinant protein was identical to the published data. In Western blot analysis the recombinant polypeptide showed excellent antigenicity against polyclonal TGF-β1 antibody. The mature recombinant rat TGF-β1 expressed in this study provides a useful tool for future detailed structural and functional studies.

关键词： TGF-β1 recombinant expression inclusion bodies purification antigenicity.

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“教育场”语境下的医学生人文素质教育

医学与哲学（A） 2010年第8期31卷 60-62页

作者：孔宪峰辽宁医学院思想政治理论教研部辽宁锦州110036

引入"场域论"原理,通过客观分析医学生人文素质教育的现实缺位、探求医学生人文素质教育的应然效果,提出了加强医学生人文素质教育的题中应有之义。在"教育场"的语境下构建医学院校、实习医院、社区、公益性教育基地等各方协同运转的人... 详细信息

引入"场域论"原理,通过客观分析医学生人文素质教育的现实缺位、探求医学生人文素质教育的应然效果,提出了加强医学生人文素质教育的题中应有之义。在"教育场"的语境下构建医学院校、实习医院、社区、公益性教育基地等各方协同运转的人文素质教育场,达到联手加强医学生人文素质教育的终极目的。

关键词：教育场医学生人文素质教育

Research progress on pharmacology and adverse reactions of gastrodin

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Asian Toxicology Research 2020年第4期2卷 170-175页

作者： Peng Lu Ke Xiong Ming Huang Bing Zhang xian-Bin kong State Key Laboratory of Component-based Chinese Medicine Tianjin University of Traditional Chinese MedicineTianjin301617China Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique Ministry of EducationTianjin301617China School of Food and Biology Engineering Xuzhou University of TechnologyJiangsu221018China College of traditional Chinese medicine Tianjin University of traditional Chinese MedicineTianjin301617China

Gastrodin(4-hydroxymethylphenyl-β-d-glucopyranoside)is an active ingredient with high content,which is isolated from the rhizome of Gastrodia *** injections,tablets and capsules are currently the main gastrodin-related preparations in clinical practice,and gastrodin injections are the most widely *** article will summarize pharmacology and adverse reactions of gastrodin to evaluate its safety,effectiveness and quality *** has pharmacological effects on central nervous system(vertigo,headache,insomnia,neurasthenia,convulsions and epilepsy,etc.),cardiovascular system and other diseases(osteoporosis,osteoarthritis and bone injury,etc.),but we still need to pay attention to its clinical adverse reactions(some patients may have dry mouth and nose,dizziness and stomach discomfort).In addition,further research is needed in other fields,such as health products,food,cosmetics,*** also need to further develop and explore the new pharmacological effects of gastrodin and expand its medicinal value.

关键词： Gastrodin Pharmacology Clinical application Adverse reactions Effectiveness

含机构位移起重机主副臂组合臂架结构几何非线性分析

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工程力学 2015年第7期32卷 210-218页

作者：王刚齐朝晖孔宪超大连理工大学工业装备结构分析国家重点实验室辽宁大连116024

工程中很多起重机械含有控制吊重位置的机构,这些附加机构约束了起重机臂架结构承载变形后的位置。机构的作用可以等效为施加到结构上的广义外力,这些外力与结构位移和机构自由度是高度耦合的。对臂架结构按长度方向划分了多个子结构,... 详细信息

工程中很多起重机械含有控制吊重位置的机构,这些附加机构约束了起重机臂架结构承载变形后的位置。机构的作用可以等效为施加到结构上的广义外力,这些外力与结构位移和机构自由度是高度耦合的。对臂架结构按长度方向划分了多个子结构,将子结构的内部位移凝聚到了左右端面上。基于共旋坐标法,建立了随子结构一起运动的随动坐标系,推导了子结构单元的广义节点内力。以履带式起重机的主副臂组合臂架结构为例,考虑到臂架需要通过变幅机构进行约束和控制,给出了机构位移附加在臂架上的广义节点外力及其导数。最后,通过起重机的副臂工况算例,给出了在不同载荷下臂架结构位移以及约束机构位移,验证了分析方法的正确性和合理性。

关键词：履带式起重机主副臂组合臂架结构机构位移几何非线性子结构共旋坐标法

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Construction of retroviral vectors to induce a strong expression of human class Ⅰ interferon gene in human hepatocellular carcinoma cells in vitro

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World Journal of Gastroenterology 1997年第3期3卷 11-14页

作者： CAO Guang Wen 1, GAO Jun 1, DU Ping 1, QI Zhong Tian 1 and kong xian Tao 2

AIM To establish the hepatoma cell specific expression of human interferon gene mediated by retroviral vectors. METHODS Human interferon α and interforon β complementary DNA (IFNs cDNA) were cloned into polylinker site of pMNSM retroviral vector to construct recombinant retroviral vector pMNSIFNA and pMNSIFNB, where the transcription of IFN gene was driven by SV40 early region promoter, and pMNAIFNA, pAMNSIFNA and pMNAIFNB, where the transcription of IFN gene was driven by SV40 early region promoter regulated by α fetoprotein enhancer. The retroviral constructs were respectively introduced into retroviral amphotropic packaging cells by means of lipofectamine mediated gene transfer procedure. The plasmids transfection rate was (4～40)×10 3 colonies/μg DNA/10 6 PA317 cells. The retrovirus infection rate was (5～500)×10 4 colony forming units (CFU)/ml. The recombinant retroviruses were used to infect human hepatoma cells, renal carcinoma cells and melanoma cell lines in the presence of 4mg/L polybrene. RESULTS Northern and Dot hybridization of total RNA from the neomycin resistant colonies and interferon expression assay indicated that human α fetoprotein enhancer induced efficient and apecific transcription and expression of IFNs gene driven by the promoter of different origin in human hepatoma cells by which α fetoprotein was highly produced. CONCLUSION Cis active element of α fetoprotein gene can drive specific expression of IFNs gene in human hepatoma cells, which provides some valuable data for the hepatoma specific immune gene therapy.

关键词： interferon alpha interferon beta retroviridae carcinoma, hepatocellular liver neoplasms genes, regulatory gene expression gene therapy