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Disulfide bridge-targeted metabolome mining unravels an antiparkinsonian peptide

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Acta Pharmaceutica Sinica B 2024年第2期14卷 881-892页

作者： Zhiwu Tong xiahong Xie Huiming Ge Ruihua Jiao Tingting Wang Xincun Wang Wenying Zhuang Gang Hu Renxiang Tan State Key Laboratory of Pharmaceutical Biotechnology Institute of Functional BiomoleculesSchool of Life SciencesNanjing UniversityNanjing 210023China State Key Laboratory Cultivation Base for TCM Quality and Efficacy Nanjing University of Chinese MedicineNanjing 210023China Institute of Microbiology Chinese Academy of SciencesBeijing 100101China

Peptides are a particular molecule class with inherent attributes of some small-molecule drugs and macromolecular biologics,thereby inspiring continuous searches for peptides with therapeutic and/or agrochemical ***,the success rate is decreasing,presumably because many interesting but less-abundant peptides are so scarce or labile that they are likely‘overlooked’during the characterization ***,we present the biochemical characterization and druggability improvement of an unprecedented minor fungal RiPP(ribosomally synthesized and post-translationally modified peptide),named acalitide,by taking the relevant advantages of metabolomics approach and disulfide-bridged substructure which is more frequently imprinted in the marketed peptide drug *** is biosynthetically unique in the macrotricyclization via two disulfide bridges and a protease(AcaB)-catalyzed lactamization of AcaA,an unprecedented precursor *** a biosynthetic logic was successfully re-edited for its sample supply renewal to facilitate the identification of the in vitro and in vivo antiparkinsonian efficacy of acalitide which was further confirmed safe and rendered brain-targetable by the liposome encapsulation *** together,the work updates the mining strategy and biosynthetic complexity of RiPPs to unravel an antiparkinsonian drug candidate valuable for combating Parkinson’s disease that is globally prevailing in an alarming manner.

关键词： Fungal RiPPs Biosynthesis Macrocyclic peptide Acalitide Antiparkinsonian

Arbuscular mycorrhiza and plant growth promoting endophytes facilitates accumulation of saponin under moderate drought stress

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Chinese Herbal Medicines 2024年第2期16卷 214-226页

作者： Shubiao Qian Qing Zhang Sha Li Rui Shi xiahong He Shuhui Zi Tao Liu Yunnan Agricultural University Kunming 650201China National&Local Joint Engineering Research Center on Gemplasm Utilization&Innovation of Chinese Medicinal Materials in Southwest China Yunnan Agricultural UniversityKunming 650201China Institute of Sericulture and Honeybee Honghe 661101China Southwest Forestry University Kunming 650201China

Objective: Paris polyphylla var. yunnanensis, one of the important medicinal plant resources in Yunnan,China, usually takes 6–8 years to be harvested. Therefore, it is urgent to find a method that can not only shorten its growth years, but also improve its quality. In this study, we examined the effects of a combination treatment of arbuscular mycorrhizal fungi(AMF) and plant growth-promoting endophytes(PGPE)and drought stress on the accumulation of saponins in ***: P. polyphylla var. yunnanensis was infected with a mixture of AMF and PGPE under drought stress. The content of saponins, as well as morphological, physiological, and biochemical indicators, were all measured. The UGTs gene related to saponin synthesis was obtained from transcriptome data by homologous comparison, which were used for RT-PCR and phylogenetic ***: Regardless of water, AMF treatment could infect the roots of P. polyphylla var. yunnanensis, however double inoculation with AMF and PGPE(AMF + PGPE) would reduce the infection rate of AMF. Plant height, aboveground and underground fresh weight did not differ significantly between the single inoculation AMF and the double inoculation treatment under different water conditions, but the inoculation treatment significantly increased the plant height of P. polyphylla var. yunnanensis compared to the noninoculation treatment. Single inoculation with AMF considerably increased the net photosynthetic rate,stomatal conductance, and transpiration rate of P. polyphylla var. yunnanensis leaves under various water conditions, but double inoculation with AMF + PGPE greatly increased the intercellular CO2concentration and chlorophyll fluorescence parameter(Fv/Fm). Under diverse water treatments, single inoculation AMF had the highest proline content, whereas double inoculation AMF + PGPE may greatly improve the amount of abscisic acid(ABA) and indoleacetic acid(IAA) compared to normal water under moderate drought. Double inoculation AMF + P

关键词： arbuscular mycorrhizal fungi gene expression Paris polyphylla Smith var.yunnanensis (Franch.)Hand.-Mazz plant growth-promoting endophytes steroidal saponin

Hypoxia-preconditioned bone marrow-derived mesenchymal stem cells protect neurons from cardiac arrest-induced pyroptosis

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Neural Regeneration Research 2025年第4期20卷 1103-1123页

作者： xiahong Tang Nan Zheng Qingming Lin Yan You Zheng Gong Yangping Zhuang Jiali Wu Yu Wang Hanlin Huang Jun Ke Feng Chen Shengli Clinical Medical College of Fujian Medical University Fujian Medical UniversityFuzhouFujian ProvinceChina Department of Emergency Fujian Provincial HospitalFuzhouFujian ProvinceChina Fujian Provincial Key Laboratory of Emergency Medicine FuzhouFujian ProvinceChina The Second Department of Intensive Care Unit Fujian Provincial Hospital South BranchFuzhouFujian ProvinceChina

Cardiac arrest can lead to severe neurological impairment as a result of inflammation,mitochondrial dysfunction,and post-cardiopulmonary resuscitation neurological *** preconditioning has been shown to improve migration and survival of bone marrow–derived mesenchymal stem cells and reduce pyroptosis after cardiac arrest,but the specific mechanisms by which hypoxia-preconditioned bone marrow–derived mesenchymal stem cells protect against brain injury after cardiac arrest are *** this end,we established an in vitro co-culture model of bone marrow–derived mesenchymal stem cells and oxygen–glucose deprived primary neurons and found that hypoxic preconditioning enhanced the protective effect of bone marrow stromal stem cells against neuronal pyroptosis,possibly through inhibition of the MAPK and nuclear factor κB ***,we transplanted hypoxia-preconditioned bone marrow–derived mesenchymal stem cells into the lateral ventricle after the return of spontaneous circulation in an 8-minute cardiac arrest rat model induced by *** results showed that hypoxia-preconditioned bone marrow–derived mesenchymal stem cells significantly reduced cardiac arrest–induced neuronal pyroptosis,oxidative stress,and mitochondrial damage,whereas knockdown of the liver isoform of phosphofructokinase in bone marrow–derived mesenchymal stem cells inhibited these *** conclude,hypoxia-preconditioned bone marrow–derived mesenchymal stem cells offer a promising therapeutic approach for neuronal injury following cardiac arrest,and their beneficial effects are potentially associated with increased expression of the liver isoform of phosphofructokinase following hypoxic preconditioning.

关键词： bone marrow–derived mesenchymal stem cells cardiac arrest cardiac resuscitation hypoxic preconditioning liver isoform of phosphofructokinase mitochondria neuroinflammation oxidative stress pyroptosis reactive oxygen species

临床护理带教老师“驾照式记分”过程管理方案的应用研究

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中国继续医学教育 2024年第7期16卷 124-129页

作者：陈敏黄霞红辜梦玲周静茹李舟德阳市人民医院重症医学科四川德阳618000

目的探讨临床护理带教老师“驾照式记分”过程管理方案在实习护生的临床带教中的应用效果。方法采用便利抽样法选取2021年1-12月德阳市人民医院危重症片区的66名临床护理带教老师、85名护生作为研究对象作为研究对象,随机分为对照组和... 详细信息

目的探讨临床护理带教老师“驾照式记分”过程管理方案在实习护生的临床带教中的应用效果。方法采用便利抽样法选取2021年1-12月德阳市人民医院危重症片区的66名临床护理带教老师、85名护生作为研究对象作为研究对象,随机分为对照组和观察组。对照组采用常规的教学管理方法;观察组在对照组的基础上实施“驾照式记分”过程管理方案。比较2组临床护理带教老师教学能力、护生实习结束后理论和操作技能考核成绩、带教老师满意度情况。结果观察组教学素质[(3.07±0.42)分]、教学内容/技能[(3.50±0.29)分]、教学方法[(3.27±0.41)分]、教学态度[(3.82±0.25)分]得分均高于对照组[(2.77±0.34)、(3.30±0.29)、(3.05±0.42)、(3.63±0.28)分],差异有统计学意义(P<0.05)。观察组护生实习结束后理论考试成绩[(84.97±6.73)分]、操作技能考试成绩[(7.84±0.51)分]和对带教老师的满意度评分[(4.68±0.09)分]均高于对照组[(81.44±9.35)分、(7.56±0.70)分、(4.20±0.06)分],差异有统计学意义(P<0.05)。结论通过临床护理带教老师“驾照式记分”过程管理方案的实施,有效提高了临床护理带教老师的教学能力和带教满意度,并提升了实习生的理论知识和技能操作水平,该方案通过量化、持续性的监督和相应的惩罚来规范和促进教学工作的健康发展。

关键词：临床教学临床护理带教老师驾照式记分过程管理实习护生

MXene材料在重金属电化学检测应用中的研究进展

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复合材料学报 2024年第6期41卷 2815-2828页

作者：李上上王洪梅何开雨王柳蓝航镇徐霞红宁波大学食品与药学学院省部共建农产品质量安全危害因子与风险防控国家重点实验室宁波315832 浙江省农业科学院农产品质量安全与营养研究所省部共建农产品质量安全危害因子与风险防控国家重点实验室杭州310021

检测重金属污染已成为风险防控、农业绿色发展、食品安全及生态保护等领域重要的技术保障。目前有许多方法来检测重金属离子,其中电化学检测法对重金属离子的检测具有灵敏度高、分析速度快、可同时对多种金属离子进行检测等优点,成为了... 详细信息

检测重金属污染已成为风险防控、农业绿色发展、食品安全及生态保护等领域重要的技术保障。目前有许多方法来检测重金属离子,其中电化学检测法对重金属离子的检测具有灵敏度高、分析速度快、可同时对多种金属离子进行检测等优点,成为了重金属快速检测领域的研究热点。MXene是具有类石墨烯结构的过渡金属碳/氮化物材料,它具备良好的亲水性、导电性和丰富可调节的终端基团。本文综述了MXenes在电化学检测重金属离子领域的研究进展:对重金属污染物的来源、危害和检测方法进行了简述;其次,概述MXene的合成方法,并重点综述了近年来MXene电化学检测重金属的研究进展及其传感机制和检测性能分析;最后,讨论了MXene材料在电化学检测重金属领域面临的挑战和展望。

关键词：重金属 MXene 电化学传感器检测食品安全

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基于科研项目的大学生创新创业能力培养研究

科技创业月刊 2024年第2期37卷 141-146页

作者：王夏虹李敏朱丽昆明医科大学科学技术处云南大学政府管理学院云南昆明650000

大学生创新创业能力是教育现代化背景下人才培养的必然要求,参与科研项目是促进创新创业能力培养的有效途径。随机选取S市某医科大学学生作为研究对象,对科研项目参与情况及创新创业能力培养现状进行问卷调查,并运用SPSSPRO进行数据分析... 详细信息

大学生创新创业能力是教育现代化背景下人才培养的必然要求,参与科研项目是促进创新创业能力培养的有效途径。随机选取S市某医科大学学生作为研究对象,对科研项目参与情况及创新创业能力培养现状进行问卷调查,并运用SPSSPRO进行数据分析,结果发现,目前创新创业能力培养存在科研项目参与度不高、学生内驱力不足、学校创新创业教育体系不够完善等问题。针对存在的问题,提出依托科研项目促进大学生创新创业能力培养的建议。

关键词：科研项目创新创业能力创新人才

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基于口渴安全策略的集束化干预措施在气管插管患者中的应用

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中外医疗 2024年第11期43卷 158-161,166页

作者：刘豪黄霞红冯华德阳市人民医院重症医学科四川德阳618000

目的探讨基于口渴安全策略的集束化干预措施在经口气管插管患者口渴干预中的应用价值。方法随机选取2022年1—12月德阳市人民医院ICU收治的80例行经口气管插管术的患者为研究对象,采用随机数表法分为对照组和观察组,每组40例。对照组接... 详细信息

目的探讨基于口渴安全策略的集束化干预措施在经口气管插管患者口渴干预中的应用价值。方法随机选取2022年1—12月德阳市人民医院ICU收治的80例行经口气管插管术的患者为研究对象,采用随机数表法分为对照组和观察组,每组40例。对照组接受常规护理,当患者出现口渴时使用棉签蘸湿口唇。观察组在常规护理的基础上,实施基于口渴安全策略的集束化干预措施。比较两组患者的口渴程度、口腔黏膜湿润程度和不良事件的发生情况。结果干预后,观察组的口渴评分(3.48±1.32)分低于对照组(6.07±1.51)分,差异有统计学意义(t=8.202,P0.05)。结论基于口渴安全策略的集束化干预措施在临床应用中效果显著,能够减轻患者口渴感受,改善口腔黏膜湿润程度,并且不会增加诸如呛咳、误吸等相关不良事件的发生。

关键词：口渴护理经口气管插管集束化干预措施重症监护室

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含刚玉变沉积岩对扬子克拉通西北缘新元古代演化史的制约

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地质科学 2024年第4期59卷 940-960页

作者：张易王浩铮冯林峰刘恒薛帅朕郭杰崔夏红西南石油大学油气藏地质及开发工程全国重点实验室地球科学与技术学院成都610500 西南石油大学火成岩综合地质研究所天然气地质四川省重点实验室成都610500 河北地质大学石家庄050031

厘清扬子西北缘出露的前寒武纪变质基底对于揭示扬子克拉通新元古代的大地构造演化历史和四川盆地的深层资源形成与赋存机制具有基础性意义。本文报道了在扬子克拉通西北缘米仓山地体中识别出的黑云母斜长片麻岩与含刚玉矽线堇青云母片... 详细信息

厘清扬子西北缘出露的前寒武纪变质基底对于揭示扬子克拉通新元古代的大地构造演化历史和四川盆地的深层资源形成与赋存机制具有基础性意义。本文报道了在扬子克拉通西北缘米仓山地体中识别出的黑云母斜长片麻岩与含刚玉矽线堇青云母片岩。相平衡模拟研究认为刚玉的产生与深熔过程中SiO2的丢失有关。黑云母斜长片麻岩记录的峰期变质温压条件达到759℃~770℃,0.48~0.55 GPa(地温梯度约为46℃/km~53℃/km),含刚玉矽线堇青云母片岩的典型矿物组合(斜长石+钛铁矿+黑云母+堇青石+刚玉+矽线石)所记录的温压条件为580℃~620℃,0.36~0.46 GPa(地温梯度范围为35℃/km~43℃/km)。变质锆石年代学揭示了两组变质年龄分别为814±9 Ma、736±17 Ma,但两组变质年龄的关系还有待进一步厘清。结合前人研究成果,本文认为含刚玉变沉积岩进一步精细刻画了自~814 Ma后的弧-陆碰撞过程,高地温梯度可能与碰撞过程中伴随幔源物质参与有关,相应的碰撞过程进一步证实扬子克拉通位于罗迪尼亚超大陆周缘。

关键词：含刚玉变沉积岩扬子克拉通新元古代变质作用

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林下人参不同部位黄酮类成分分析

南方农业 2024年第4期18卷 88-94页

作者：周晓敏何霞红施蕊李顺美张靖仪云南省林下资源保护与利用重点实验室云南昆明650224 西南林业大学园林园艺学院云南昆明650224

为明确林下人参不同部位黄酮类成分含量,提高人参的综合利用水平。运用超高效液相色谱-串联质谱(UPLC-MS/MS)技术分析了林下栽培4年人参的根、茎、叶的黄酮类成分,并通过代谢组学的方法筛选出不同部位的差异代谢物。结果表明:林下人参... 详细信息

为明确林下人参不同部位黄酮类成分含量,提高人参的综合利用水平。运用超高效液相色谱-串联质谱(UPLC-MS/MS)技术分析了林下栽培4年人参的根、茎、叶的黄酮类成分,并通过代谢组学的方法筛选出不同部位的差异代谢物。结果表明:林下人参根、茎、叶中共检测出92种黄酮类代谢物,包括41种黄酮醇、13种黄酮化合物、11种二氢黄酮、7种花青素、5种黄酮碳糖苷、5种异黄酮、5种查耳酮、4种二氢黄酮醇、1种其他类黄酮;并通过主成分分析(Principal Component Analysis,PCA)和正交偏最小二乘判别分析法(Partial Least Squares-Discriminant Analysis,PLSDA)筛选出27种差异代谢物,其中15种代谢物在林下人参各部位的含量从高到低依次为茎>叶>根,5种代谢物在林下人参各部位的含量从高到低依次为根>茎>叶,4种代谢物在林下人参各部位的含量从高到低依次为叶>根>茎,2种代谢物在林下人参各部位的含量从高到低依次为叶>茎>根,1种代谢物在林下人参各部位的含量从高到低依次为茎>根>叶。总的来说,人参不同部位黄酮类成分存在差异,可为人参的进一步研究与合理应用提供参考。

关键词：人参黄酮类成分不同部位代谢组学

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