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Organic Chemistry Frontiers 2022年第4期9卷 1143-1151页

作者： xiahe chen Chaoqin Cao Yun-Fang Yang Yuan-Bin She College of Chemical Engineering Zhejiang University of TechnologyHangzhouZhejiang 310014China

The mechanisms and regioselectivities of the Ir-porphyrin-catalyzed C–H insertion reaction of quinoid carbene(QC)were investigated by density functional theory(DFT)*** competing catalytic cycles were identified as the hydrogen-atom abstraction by quinoid oxygen(HAA-O)and hydrogen-atom abstraction by carbene carbon(HAA-C)catalytic cycles.

关键词： porphyrin insertion catalyzed

Insulin expression in livers of diabetic mice mediated by hydrodynamics-based administration

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World Journal of Gastroenterology 2004年第4期10卷 567-572页

作者： chen-xiahe DingShi Wen-JunWu You-FaDing Deng-MinFeng BinLu Hao-Mingchen Ji-HuaYao QiShen Da-RuLu Jing-LunXue StateKeyLaboratoryofGeneticEngineering InstituteofGeneticsSchoolofLifeSciencesFudanUniversityShanghai200433China

AIM:Transfer and expression of insulin gene in vivo are an alternative strategy to improve glycemic control in type 1 diabetes. Hydrodynamics-based procedure has been proved to be very efficient to transfer naked DNA to mouse livers. The basal hepatic insulin production mediated by this rapid tail vein injection was studied to determine its effect on the resumption of glycemic control in type 1 diabetic ***: Engineered insulin cDNA was inserted into plasmid vectors under a CMV promoter, and transferred into STZ induced diabetic mice by hydrodynamic *** levels, body weight of treated mice, insulin levels,immunohistology of the liver, and quantity of insulin mRNA in the liver were assayed to identify the improvement of hyperglycemic complication after plasmid *** Beauty, a transposon system, was also used to prolong the insulin expression in the ***: After plasmid administration, Plasma insulin was significantly increased in the diabetic mice and the livers were insulin-positive by immunostaining. At the same time the hyperglycemic complication was improved. The blood glucose levels of mice were reduced to normal. Glucose tolerance of the treated diabetic mice was improved. Body weight loss was also ameliorated. The rapid tail vein injection did not cause any fatal ***: Our results suggested that insulin gene could be efficiently transferred into the livers of diabetic mice via rapid tail vein injection and it resulted in high level of insulin expression. The basal hepatic insulin production mediated by hydrodynamics-based administration improved the glycemic control in type 1 diabetes dramatically and ameliorated diabetic syndromes. Hydrodynamics-based administration offers a simple and efficient way in the study of gene therapy for type 1 diabetes.

关键词：胰岛素糖尿病动物模型血流动力学基因转染肝脏组织

全程多学科营养模式在老年患者髋部手术中的应用研究

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中国现代医生 2024年第22期62卷 17-21页

作者：陈夏荷吕杨训陈琦凡林洲廖飞飞温州市中心医院骨科浙江温州325000

目的探讨全程多学科营养模式在老年患者髋部手术中的应用效果。方法选取2021年10月至2023年12月于温州市中心医院行髋部手术的老年患者90例,根据随机数字表法将其分为对照组和研究组,每组各45例。对照组患者应用饮食管理模式,研究组患... 详细信息

目的探讨全程多学科营养模式在老年患者髋部手术中的应用效果。方法选取2021年10月至2023年12月于温州市中心医院行髋部手术的老年患者90例,根据随机数字表法将其分为对照组和研究组,每组各45例。对照组患者应用饮食管理模式,研究组患者应用全程多学科营养模式。比较两组患者的恢复情况、营养状况、生活质量、髋关节功能和满意度。结果研究组患者的住院时间、骨折愈合时间均显著短于对照组(P<0.05)。干预后,两组患者的前白蛋白(prealbumin,PA)、白蛋白(albumin,Alb)、血红蛋白(hemoglobin,Hb)、健康调查量表36(36-item short form health survey,SF-36)各维度评分、Harris髋关节评分均显著高于本组干预前(P<0.05),且研究组患者的PA、Alb、Hb、SF-36各维度评分、Harris髋关节评分均显著高于对照组(P<0.05)。研究组患者的满意度显著高于对照组(χ^(2)=5.075,P=0.024)。结论老年患者髋部手术中应用全程多学科营养模式可改善其营养状态,提升生活质量,增强髋关节功能,有利于术后恢复。

关键词：多学科团队营养模式髋部手术

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强化围手术期护理在腰椎滑移行椎弓根螺钉加后路椎体间植骨治疗中的效果

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浙江医学教育 2019年第5期18卷 49-51页

作者：廖飞飞厉蕾蕾陈夏荷林洲温州市中心医院

目的:分析腰椎滑移行椎弓根螺钉加后路椎体间植骨治疗的围手术期强化护理效果。方法:于2017年1月1日到2017年12月30日期间共纳入33例接受腰椎滑移行椎弓根螺钉加后路椎体间植骨治疗的患者,对照组采用常规围手术期护理,观察组强化围手术... 详细信息

目的:分析腰椎滑移行椎弓根螺钉加后路椎体间植骨治疗的围手术期强化护理效果。方法:于2017年1月1日到2017年12月30日期间共纳入33例接受腰椎滑移行椎弓根螺钉加后路椎体间植骨治疗的患者,对照组采用常规围手术期护理,观察组强化围手术期护理,比较2组护理效果。结果:观察组愈合时间为(5.96±1.33)月,短于对照组愈合时间(7.69±1.05)月(p<0.05).护理后观察组QOL评分(39.83±6.19)分高于对照组(30.62±7.24)分,NRS评分(2.01±0.43)低于对照组(3.00±0.89)分,差异均具有统计学意义(p<0.05)。结论:针对腰椎滑移患者采用强化围手术期护理可促进骨性愈合,提高患者生活质量,缓解术后疼痛,有临床应用价值。

关键词：腰椎滑移椎弓根螺钉后路椎体间植骨围手术期护理

Controlling Effects of Tight Reservoir Micropore Structures on Seepage Ability: A Case Study of the Upper Paleozoic of the Eastern Ordos Basin, China

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Acta Geologica Sinica(English Edition) 2020年第2期94卷 322-336页

作者： YANG Bo QU Hongjun PU Renhai TIAN xiahe YANG Huan DONG Wenwu chen Yahui State Key Laboratory of Continental Dynamics Deptof GeologyNorthwest UniversityXi'an 710069China

In this study, the types of micropores in a reservoir are analyzed using casting thin section(CTS) observation and scanning electron microscopy(SEM) experiments. The high-pressure mercury injection(HPMI) and constant-rate mercury injection(CRMI) experiments are performed to study the micropore structure of the reservoir. Nuclear magnetic resonance(NMR), gas-water relative seepage, and gas-water two-phase displacement studies are performed to examine the seepage ability and parameters of the reservoir, and further analyses are done to confirm the controlling effects of reservoir micropore structures on seepage ability. The experimental results show that Benxi, Taiyuan, Shanxi, and Shihezi formations in the study area are typical ultra-low porosity and ultra-low permeability reservoirs. Owing to compaction and later diagenetic transformation, they contain few primary pores. Secondary pores are the main pore types of reservoirs in the study area. Six main types of secondary pores are: intergranular dissolved pores, intragranular dissolved pores, lithic dissolved pores, intercrystalline dissolved pores, micropores, and microfracture. The results show that reservoirs with small pore-throat radius, medium displacement pressure, and large differences in pore-throat structures are present in the study area. The four types of micropore structures observed are: lower displacement pressure and fine pores with medium-fine throats, low displacement pressure and fine micropores with fine throats, medium displacement pressure and micropores with micro-fine throats, and high displacement pressure and micropores with micro throats. The micropore structure is complex, and the reservoir seepage ability is poor in the study areas. The movable fluid saturation, range of the gas-water two-phase seepage zone, and displacement types are the three parameters that well represent the reservoir seepage ability. According to the characteristic parameters of microscopic pore structure and seepag

关键词： micro-pore structure seepage ability movable fluid saturation the range of gas-water two phase seepage zone displacement types

Microstructure induced galvanic corrosion evolution of SAC305 solder alloys in simulated marine atmosphere

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Journal of Materials Science & Technology 2020年第16期47卷 40-53页

作者： Mingna Wang Chuang Qiao Xiaolin Jiang Long Hao xiahe Liu Department of Physics Hebei Normal University of Science&TechnologyQinhuangdao 066004China School of Metallurgy Northeastern UniversityShenyang 110819China Environmental Corrosion Centre of Materials Institute of Metal ResearchChinese Academy of SciencesShenyang 110016China School of Material Science and Engineering University of Science and Technology of ChinaHefei 230026China

Motivated by the increasing use of Sn-3.0 Ag-0.5 Cu(SAC305)solder in electronics worked in marine atmospheric environment and the uneven distribution of Ag3Sn and Cu6Sn5 intermetallic compounds(IMCs)inβ-Sn matrix,comb-like electrodes have been designed for in-situ EIS measurements to study the microstructure induced galvanic corrosion evolution of SAC305 solder in simulated marine atmosphere with high-temperature and *** indicate that in-situ EIS measurement by comb-like electrodes is an effective method for corrosion evolution behavior study of SAC305 ***,the galvanic effect between Ag3Sn IMCs andβ-Sn matrix can aggravate the corrosion of both as-received and furnace-cooled SAC305 solder as the exposure time proceeds in spite of the presence of corrosion product *** corrosion can be preferentially found on furnace-cooled SAC305 with larger Ag3Sn grain ***,the generated inner stress during phases transformation process with Sn3O(OH)2Cl2 as an intermediate and the possible hydrogen evolution at local acidified sites are supposed to be responsible for the loose,porous,cracked,and non-adherent corrosion product *** findings clearly demonstrate the corrosion acceleration behavior and mechanism of SAC305 solder,and provide potential guidelines on maintenance of microelectronic devices for safe operation and longer in-service duration.

关键词： SAC305 solder Marine atmosphere Galvanic corrosion In-situ EIS Comb-like electrode

Time-and pH-dependent colon-specific drug delivery for orally administered diclofenac sodium and 5-aminosalicylic acid

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World Journal of Gastroenterology 2004年第12期10卷 1769-1774页

作者： Gangcheng FengAn Mei-JuanZou JinSun Xiu-HuaHao Yun-xiahe DepartmentofBiopharmaceutics SchoolofPharmacyShenyangPharmaceuticalUniversityShenyang110016LiaoningProvinceChina SchoolofPharmacy JilinUniversityChangchun130021JilinProvinceChina

AIM: To investigate Time- and pH-dependent colon-specific drug delivery systems (CDDS) for orally administered diclofenac sodium (DS) and 5-aminosalicylic acid (5-ASA), respectively. METHODS: DS tablets and 5-ASA pellets were coated by ethylcellulose (EC) and methacrylic acid copolymers (Eudragit L100 and $100), respectively. The in vitro release behavior of the DS coated tablets and 5-ASA coated pellets were examined, and then in vivo absorption kinetics of DS coated tablets in dogs were further studied. RESULTS: Release profile of time-dependent DS coated tablets was not influenced by pH of the dissolution medium, but the lag time of DS release was primarily controlled by the thickness of the coating layer. The thicker the coating layer, the longer the lag time of DS release is. On the contrary, in view of the pH-dependent 5-ASA coated pellets, 5-ASA release was significantly governed by pH. Moreover, the 5-ASA release features from the coated pellets depended upon both the combination ratio of the Eudragit~ L100 and S100 pH-sensitive copolymers in the coating formulation and the thickness of the coating layer. The absorption kinetic studies of the DS coated tablets in dogs demonstrated that in vivo lag time of absorption was in a good agreement with in vitro lag time of release. CONCLUSION: Two types of CDDS, prepared herein by means of the regular coating technique, are able to achieve site-specific drug delivery targeting at colon following oral administration, and provide a promising strategy to control drug release targeting the desired lower gastrointestinal region.

关键词：特效药麻醉药环氟拉嗪二氯苯二磺酰胺钠 5-对氨基水杨酸动力学

An effective system for detecting protein-protein interaction based on in vivo cleavage by PPV NIa protease

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Protein & Cell 2012年第12期3卷 921-928页

作者： Nuoyan zheng xiahe Huang Bojiao Yin Dan Wang Qi Xie Department of Nephrology the First Affiliated HospitalSun Yat-Sen UniversityGuangzhou 510000China Research Center of Translational Medicine the First Affiliated HospitalSun Yat-Sen UniversityGuangzhou 510000China State Key Laboratory of Plant Genomics National Centre for Plant Gene ResearchInstitute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China Animal Center Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China

Detection of protein-protein interaction can provide valuable information for investigating the biological function of *** current methods that applied in protein-protein interaction,such as co-immunoprecipitation and pull down etc.,often cause plenty of working time due to the burdensome cloning and puri-fication *** we established a system that characterization of protein-protein interaction was accomplished by co-expression and simply purification of target proteins from one expression cassette within *** *** modified pET vector into co-expression vector pInvivo which encoded PPV NIa protease,two cleavage site F and two multiple cloning sites that flanking cleavage *** target proteins(for example:protein A and protein B)were inserted at multiple cloning sites and translated into polyprotein in the order of MBP tag-protein A-site F-PPV NIa protease-site F-protein B-His6 *** NIa protease carried out intracellular cleavage along expression,then led to the separation of polyprotein components,therefore,the interaction between protein A-protein B can be detected through one-step purification and *** control for protein B was brought into this system for monitoring interaction *** successfully employed this system to prove two cases of reported protien-protein interaction:RHA2a/ANAC and FTA/*** conclusion,a convenient and efficient system has been successfully developed for detecting protein-protein interaction.

关键词： PPV NIa protease protein-protein interaction in vivo cleavage fusion protein

Trophic Mode-Dependent Proteomic Analysis Reveals Functional Significance of Light- Independent Chlorophyll Synthesis in Synechocystis sp. PCC 6803

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Molecular Plant 2017年第1期10卷 73-85页

作者： Longfa Fang Haitao Ge xiahe Huana Ye Liu Min Lu Jinlong Wang Weiyang chen Wu Xu Yingchun Wang State Key Laboratory of Molecular Developmental Biology Institute of Genetics and Developmental Biology Chinese Academy of Sciences No.1 West Beichen Road Beijing 100101 China Department of Chemistry University of Louisiana at Lafayette Lafayette LA 70504 USA These authors contributed equally to this article

The photosynthetic model organism Synechocystis sp. PCC 6803 can grow in different trophic modes, depending on the availability of light and exogenous organic carbon source. However, how the protein pro- file changes to facilitate the cells differentially propagate in different modes has not been comprehensively investigated. Using isobaric labeling-based quantitative proteomics, we simultaneously identified and quantified 45% Synechocystis proteome across four different trophic modes, i.e., autotrophic, heterotro- phic, photoheterotrophic, and mixotrophic modes. Among the 155 proteins that are differentially expressed across four trophic modes, proteins involved in nitrogen assimilation and light-independent chlorophyll synthesis are dramatically upregulated in the mixotrophic mode, concomitant with a dramatic increase of PII phosphorylation that senses carbon and nitrogen assimilation status. Moreover, functional study us- ing a mutant defective in light-independent chlorophyll synthesis revealed that this pathway is important for chlorophyll accumulation under a cycled light/dark illumination regime, a condition mimicking day/night cycles in certain natural habitats. Collectively, these results provide the most comprehensive information on trophic mode-dependent protein expression in cyanobacterium, and reveal the functional significance of light-independent chlorophyll synthesis in trophic growth.

关键词： proteomics phosphorylation Synechocystis light-independent chlorophyll synthesis nitrogenassimilation

Temperature and NaCl deposition dependent corrosion of SAC305 solder alloy in simulated marine atmosphere

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Journal of Materials Science & Technology 2021年第16期75卷 252-264页

作者： Chuang Qiao Mingna Wang Long Hao xiahe Liu Xiaolin Jiang Xizhong An Duanyang Li School of Metallurgy Northeastern UniversityShenyang110819China Environmental Corrosion Centre of Materials Institute of Metal ResearchChinese Academy of SciencesShenyang110016China Department of Physics Hebei Normal University of Science technologyQinhuangdao066004China School of Material Science and Engineering University of Science and Technology of China.Hefei230026China College of Mechanical Engineering Institute of Innovative Science and TechnologyShenyang UniversityShenyang110044.China

The stable operation of electronic devices in marine atmospheric environment is affected by the corrosion deterioration of solder joints,and the effects by atmosphere temperature and chloride deposition are *** this work,NaCl deposition and temperature dependent corrosion of Pb-free SAC305 solder in simulated marine atmosphere has been *** results indicate that higher NaCl deposition prolongs the surface wetting time and leads to the final thicker saturated electrolyte film for further *** temperature accelerates the evaporation and contributes to the final thinner saturated NaCl electrolyte ***,the corrosion control process varies under the initially covered thicker NaCl electrolyte layer and under the final saturated much thinner NaCl electrolyte film as the evaporation ***,the ready oxygen availability through the final thinner saturated NaCl electrolyte film facilitates the formation of corrosion product layer mainly of electrochemically stable SnO2,but higher temperature leads to the final corrosion product layer with smaller crystal size and large *** findings clearly demonstrate the effects of NaCl deposition and temperature on corrosion evolution of SAC305 solder joints and are critical to the daily maintenance of electronic devices for longer service life in marine atmosphere.

关键词： Atmospheric corrosion SAC305 solder Marine atmosphere NaCl Temperature