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Frontiers of Environmental Science & Engineering 2023年第9期17卷 137-146页

作者： Xi Luo Ao Li xue xia Peng Liang xia Huang College of Life and Environmental Sciences Minzu University of ChinaBeijing 100081China State Key Joint Laboratory of Environment Simulation and Pollution Control School of EnvironmentTsinghua UniversityBeijing 100084China Yangtze Ecology and Environment Co. Ltd.Wuhan 430062China

H_(2)O_(2) was produced at an appreciable rate in microbial reverse-electrodialysis cells(MRCs)coupled with thermolytic solutions,which can simultaneously capture waste heat as electrical *** determine the optimal cathode and membrane stack configurations for H_(2)O_(2) production,different catalysts,catalyst loadings and numbers of membrane cell pairs were *** black(CB)outperformed activated carbon(AC)for H_(2)O_(2) production,although AC showed higher catalytic activity for oxygen *** optimum CB loading was 10 mg/cm^(2) in terms of both the H_(2)O_(2) production rate and power *** optimum number of cell pairs was determined to be three based on a tradeoff between H_(2)O_(2) production and capital costs.A H_(2)O_(2) production rate as high as 0.99±0.10 mmol/(L·h)was achieved with 10 mg/cm^(2) CB loading and 3 cell pairs,where the H_(2)O_(2) recovery efficiency was 52±2%and the maximum power density was 780±37 mW/m^(2).Increasing the number of cell pairs to five resulted in an increase in maximum power density(980±21 mW/m^(2))but showed limited effects on H_(2)O_(2) *** results indicated that MRCs can be an efficient method for sustainable H_(2)O_(2) production.

关键词： Microbial reverse-electrodialysis cell Hydrogen peroxide production Ammonium bicarbonate Electrolysis cell Optimization

Electrocatalytic reduction of nitrate using Pd-Cu modified carbon nanotube membranes

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Frontiers of Environmental Science & Engineering 2023年第4期17卷 1-11页

作者： Zhijun Liu Xi Luo Senlin Shao xue xia College of Life and Environmental Sciences Minzu University of ChinaBeijing 100081China School of Civil Engineering Wuhan UniversityWuhan 430072China Yangtze Ecology and Environment Co. Ltd.Wuhan 430062China

Excessive nitrate in water is harmful to the ecological environment and human *** reduction is a promising technology for nitrate ***,a Pd-Cu modified carbon nanotube membrane was fabricated with an electrodeposition method and used to reduce nitrate in a flowthrough electrochemical *** optimal potential and duration for codeposition of Pd and Cu were-0.7 V and 5 min,respectively,according to linear scan voltammetry *** membrane obtained with a Pd:Cu ratio of 1:1 exhibited a relatively high nitrate removal efficiency and N_(2)*** was almost completely reduced(~99%)by the membrane at potentials lower than-1.2 ***,-0.8 V was the optimal potential for nitrate reduction in terms of both nitrate removal efficiency and product *** nitrate removal efficiency was 56.2%,and the N_(2)selectivity was 23.8%for the Pd:Cu=1:1 membrane operated at-0.8 *** removal was enhanced under acidic conditions,while N_(2)selectivity was *** concentrations of Cl-ions and dissolved oxygen showed little effect on nitrate *** mass transfer rate constant was greatly improved by 6.6 times from 1.14×10^(-3)m/h at a membrane flux of 1 L/(m^(2)·h)to 8.71×10^(-3)m/h at a membrane flux of 15 L/(m^(2)·h),which resulted in a significant increase in the nitrate removal rate from 13.6 to 133.5 mg/(m^(2)·h).These findings show that the Pd-Cu modified CNT membrane is an efficient material for nitrate reduction.

关键词： Pd-Cu modified CNT membrane Nitrate reduction Flow-through Electrodeposition Electrocatalytic reduction

Crystal Structure of a Novel Complex Na_4[Y(PDC)_2(BTC)]·5H_2O with One-Dimensional Helical Network Structure (H2PDC = 2,6-Pyridinedicarboxylic acid)( H_3BTC = 1,3,5-Benzenetricarboxylic acid )

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Chinese Journal of Structural Chemistry 2005年第10期24卷 1181-1185页

作者： xue Xu-Fei CHE Yun-xia xue Lin ZHENG Ji-Min Department of Chemistry Nankai University Tianjin 300071 China

A novel title complex, C23H19N2O19Na4Y （Mr= 808.27）, has been synthesized by hydrothermal reaction and structurally characterized by X-ray diffraction. It crystallizes in monoclinic, space group P21/n with a = 13.560（3）, b = 14.016（3）, c = 14.948（4） A, β = 99.492（4）°, Z = 4, V = 2802.2（12） A^3, Dc = 1.916 g/cm^3,μ（MoKα） = 2.241 mm^-1, F（000） = 1624, the final R = 0.0337 and wR = 0.0828 for 4044 observed reflections（I〉 2σ（I））. In the crystal, each eight- coordinated Y（Ⅲ） atom is linked by carboxyl groups of BTC to form a one-dimensional helical network structure.

关键词： 1,3,5-benzenetricarboxylic acid 2,6-pyridinedicarboxylic acid,hydrothermal synthesis crystal structure

COVID-19 in children and the importance of COVID-19 vaccination

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World Journal of Pediatrics 2021年第5期17卷 462-466页

作者： Feng-xia xue Kun-Ling Shen Department of Respiratory Medicine Beijing Children's HospitalChina National Clinical Research Center of Respiratory DiseasesNational Center for Children's HealthCapital Medical UniversityBeijing 100045China

As of August 30,2021,COVID-19 has caused more than 216 million infections and 4.49 million deaths worldwide,with 0.58 million new cases[1].Because the COVID-19 vaccine is distributed gradually and widely,the development of the global epidemic has been prevented to some extent,but the emergence of SARS-CoV-2 variants makes us *** alpha(B.1.1.7),beta(B.1.351),gamma(P.1)and delta(B.1.617)strains have been named variants of concern by the WHO[2].The SARS-CoV-2 variants have increased in transmissibility and virulence,leading to higher morbidity and mortality.

关键词： COV milli delta

Feasibility, limitation and possible solutions of RNAi-based technology for insect pest control

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Insect Science 2013年第1期20卷 15-30页

作者： Hao Zhang Hai-Chao Li xue-xia Miao Key Laboratory of Insect Developmental and Evolutionary Biology Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate University of the Chinese Academy of Sciences Shanghai China

Numerous studies indicate that target gene silencing by RNA interference （RNAi） could lead to insect death. This phenomenon has been considered as a potential strategy for insect pest control, and it is termed RNAi-mediated crop protection. However, there are many limitations using RNAi-based technology for pest control, with the effectiveness target gene selection and reliable double-strand RNA （dsRNA） delivery being two of the major challenges. With respect to target gene selection, at present, the use of homologous genes and genome-scale high-throughput screening are the main strategies adopted by researchers. Once the target gene is identified, dsRNA can be delivered by micro-injection or by feeding as a dietary component. However, micro-injection, which is the most common method, can only be used in laboratory experiments. Expression of dsRNAs directed against insect genes in transgenic plants and spraying dsRNA reagents have been shown to induce RNAi effects on target insects. Hence, RNAi-mediated crop protection has been considered as a potential new-generation technology for pest control, or as a complementary method of existing pest control strategies; however, further devel- opment to improve the efficacy of protection and range of species affected is necessary. In this review, we have summarized current research on RNAi-based technology for pest insect management. Current progress has proven that RNAi technology has the potential to be a tool for designing a new generation of insect control measures. To accelerate its practical application in crop protection, further study on dsRNA uptake mechanisms based on the knowledge of insect physiology and biochemistry is needed.

关键词： delivery dsRNA insect pest control RNAi screen

PEGylated bacteria restore intestinal mucosal barrier

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Acta Pharmaceutica Sinica B 2024年第9期14卷 4186-4188页

作者： Menghang Zu xue xia Bo xiao State Key Laboratory of Resource Insects College of SericultureTextileand Biomass SciencesSouthwest UniversityChongqing 400715China Department of Pharmacy Personalized Drug Therapy Key Laboratory of Sichuan ProvinceSichuan Provincial People’s HospitalSchool of MedicineUniversity of Electronic Science and Technology of ChinaChengdu 610054China

The mucosal barrier of the gastrointestinal tract(GIT)is crucial for the overall health of the *** disruption permits the infiltration of intestinal microorganisms into the underlying mucosa,resulting in infections and inflammatory conditions,such as inflammatory bowel disease(IBD),metabolic disorders,and autoimmune diseases^(1,2).Therefore,strategies to protect the mucosal barrier from exogenous stimuli or to facilitate its recovery have become increasingly ***,immunomodulators,corticosteroids,and biological agents have been widely employed to treat IBD by alleviating inflammation and enhancing mucosal ***,they can also produce serious systemic toxicity^(3,4);therefore,there is an urgent need for exploiting innovative strategies to fortify the intestinal mucosal barrier against the invasion of exogenous damaging factors.

关键词： PEGylation Probiotic Mucus penetration Colonic mucosal barrier Colitis Diabetes

Identification and Expression of Floral Organ Homeotic Genes from Alpinia oblongifolia (Zingiberaceae)

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Journal of Integrative Plant Biology 2009年第2期51卷 155-166页

作者： Yong-Mei xia xue-Mei Gao Qing-Jun Li Xishuangbanna Tropical Botanical Garden the Chinese Academy of Sciences Menglun Mengla Yunnan 666303 China

Current understanding of the classical ABC model of floral development has provided a new set of characters to evaluate floral evolution. However, what is still lacking is a clear assessment of this genetic program across monocots. Here, to investigate the evolution of members of class A and B genes in monocots, we report the sequence characteristic and transcript expression of three new MADS-box genes in Alpinia oblongifolia Hayata. Sequence and phylogenetic analysis reveals that these genes are FUL-like and AP3-1ike. Therefore, they were termed AoFL1, AoFL2 and AoAP3. AoFL1 contains the FUL motif, but AoFL2 lacks this motif. Their expression revealed by in situ hybridization may reflect the ancestral function of FUL-like genes in the specification of inflorescence and floral meristems. The AoAP3 gene contains two conserved motifs, the PI-derived and paleoAP3 motifs. The AoAP3 transcripts located to the corolla and stamen, and hybridization signals were detected in the central whorl. These expression patterns suggest that the functions of homologous organ identity genes are diversified in A. oblongifolia. The implications of these findings on the conservation of homologous gene function are discussed.

关键词： ABC model floral organ identity in situ hybridization MADS-box genes phylogenetic analysis.

Prediction of effective RNA interference targets and pathway-related genes in lepidopteran insects by RNA sequencing analysis

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Insect Science 2018年第3期25卷 356-367页

作者： Ruo-Bing Guan Hai-Chao Li xue-xia Miao Key Laboratory of Insect Developmental and Evolutionary Biology Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China

When using RNA interference （RNAi） to study gene functions in Lepidoptera insects, we discovered that some genes could not be suppressed; instead, their expression levels could be up-regulated by double-stranded RNA （dsRNA）. To predict which genes could be easily silenced, we treated the Asian corn borer （Ostriniafilrnacalis） with dsGFP （green fluorescent protein） and dsMLP （muscle lim protein）. A transcriptome sequence analysis was conducted using the cDNAs 6 h after treatment with dsRNA. The results indicated that 160 genes were up-regulated and 44 genes were down-regulated by the two dsRNAs. Then, 50 co-up-regulated, 25 co-down-regulated and 43 unaffected genes were selected to determine their RNAi responses. All the 25 down-regulated genes were knocked down by their corresponding dsRNA. However, several of the up-regulated and unaffected genes were up-regulated when treated with their corresponding dsRNAs instead of being knocked down. The genes up-regulated by the dsGFP treatment may be involved in insect immune responses or the RNAi pathway. When the immune-related genes were excluded, only seven genes were induced by dsGFP, including ago-2 and dicer-2. These results not only provide a reference for efficient RNAi target predications, but also provide some potential RNAi pathway-related genes for further study.

关键词： Ostrinia furnacalis RNA interference RNAi targets RNA-seq

Deficiency of mitochondrial outer membrane protein 64 confers rice resistance to both piercing-sucking and chewing insects in rice

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Journal of Integrative Plant Biology 2020年第12期62卷 1967-1982页

作者： Hui‐Min Guo Hai‐Chao Li Shi‐Rong Zhou Hong‐Wei xue xue‐xia Miao Key Laboratory of Insect Developmental and Evolutionary Biology CAS Center for Excellence in Molecular Plant SciencesShanghai Institute of Plant Physiology and EcologyInnovation Academy for Seed DesignChinese Academy of SciencesShanghai 200032China National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesShanghai Institute of Plant Physiology and EcologyChinese Academy of SciencesShanghai 200032China

The brown planthopper(BPH)and striped stem borer(SSB)are the most devastating insect pests in rice(Oryza sativa)producing *** for endogenous resistant genes is the most practical strategy for rice insect-resistance ***-five mutants showing high re-sistance against BPH were identified in a rice T-DNA in-sertion population(11,000 putative homozygous lines)after 4 years of large-scale field BPH-resistance phenotype *** analysis showed that deficiency of rice mitochondrial outer membrane protein 64(OM64)gene resulted in increased resistance to *** outer membrane protein 64 protein is located in the outer mitochondrial membrane by subcellular localization and its deficiency constitutively activated hydrogen peroxide(H2O2)signaling,which stimulated antibiosis and tolerance to *** om64 mutant also showed enhanced resist-ance to SSB,a chewing insect,which was due to promotion of Jasmonic acid biosynthesis and related ***-portantly,om64 plants presented no significant changes in rice yield-related *** study confirmed OM64 as a negative regulator of rice herbivore resistance through regulating H2O2 *** outer membrane protein 64 is a potentially efficient candidate to improve BPH and SSB resistance through gene *** the om64 mutant was resistant to both piercing-sucking and chewing insects via a gene deficiency in mitochondria is discussed.

关键词： sativa) fir resistance

Microbiome changes in esophageal cancer:implications for pathogenesis and prognosis

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Cancer Biology & Medicine 2024年第2期21卷 163-174页

作者： Yi Li Bing Wei xia xue Hongle Li Jun Li Department of Molecular Pathology Clinical Pathology CenterAffiliated Cancer Hospital of Zhengzhou University and Henan Cancer HospitalZhengzhou 450003China Henan Key Laboratory of Molecular Pathology Zhengzhou 450003China Henan Key Laboratory of Helicobacter pylori&Microbiota and Gastrointestinal Cancer Marshall Medical Research CenterThe Fifth Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052China

Esophageal cancer(EC)is an aggressive malignancy with a poor *** factors,including dietary habits,and antacid and antibiotic use,have been shown to influence the esophageal ***,enrichment and diversity of the esophageal microbiome can also impact its *** studies have revealed prevalent changes in the esophageal microbiome among patients with EC,thus suggesting the potential contribution of the esophageal microbiome to EC ***,distinct microbiome compositions have been observed in patients with different responses to radiotherapy and chemotherapy,indicating the role of the esophageal microbiome in modulating treatment *** this review,we have examined previous studies on the esophageal microbiome in healthy individuals and patients with EC or other esophageal diseases,with a focus on identifying microbial communities associated with EC pathogenesis and *** the role of the microbiome in EC may aid in early detection and optimized treatment strategies,ultimately leading to better outcomes for patients.

关键词： Esophageal cancer microbiome dysbiosis microenvironment carcinogenesis