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探讨中医面部调阳健脑抗衰美容手法的临床应用

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中国美容医学 2024年第9期33卷 74-78页

作者：曹燕燕惠建荣王星星肖燕彭贞陕西中医药大学陕西咸阳712046 陕西中医药大学附属医院针灸科陕西咸阳712000

目的:本文概括总结惠建荣教授头面部调阳健脑抗衰美容功法经验,阐述了惠建荣教授提出的七步头面部调阳健脑抗衰美容功法具体内容,即“提拉揉颈法”“放松口唇法”“鼻部养颜法”“醒目亮眼法”“祛皱美额法”“聪耳健身法”“调阳健脑法... 详细信息

目的:本文概括总结惠建荣教授头面部调阳健脑抗衰美容功法经验,阐述了惠建荣教授提出的七步头面部调阳健脑抗衰美容功法具体内容,即“提拉揉颈法”“放松口唇法”“鼻部养颜法”“醒目亮眼法”“祛皱美额法”“聪耳健身法”“调阳健脑法”,并总结了该功法作用特色,即重在增强阳气、健脑益智及美容抗衰,并附验案一则,将理论与临床美容抗衰相结合,为中医美容抗衰的实践研究提供新方法、新思路。

关键词：推拿调阳健脑抗衰美容经验

Toxicity and metabolism of 3-bromopyruvate in Caenorhabditis elegans

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2020年第1期21卷 77-86页

作者： Qiao-ling GU Yan ZHANG xi-mei FU Zhao-lian LU Yao YU Gen CHEN Rong ma Wei KOU Yong-mei LAN The Institute of Minority Physique and Health Medical College of Northwest University for NationalitiesLanzhou 730030China Department of Anesthesiology First HospitalLanzhou UniversityLanzhou 730000China Pathogen Biology Institute School of Basic Medical SciencesLanzhou UniversityLanzhou 730000China Department of Microbiology School of Basic Medical SciencesGuilin Medical UniversityGuilin 541100China

In this study,we aimed to evaluate the toxic effects,changes in life span,and expression of various metabolismrelated genes in Caenorhabditis elegans,using RNA interference(RNAi)and mutant strains,after 3-bromopyruvate(3-BrPA)*** was treated with various concentrations of 3-BrPA on nematode growth medium(NGM)plates,and their survival was monitored every 24 *** expression of genes related to metabolism was measured by the real-time fluorescent quantitative polymerase chain reaction(qPCR).Nematode survival in the presence of 3-BrPA was also studied after silencing three hexokinase(HK)*** average life span of *** cultured on NGM with 3-BrPA was shortened to 5.7 d compared with 7.7 d in the control ***-1,hxk-2,and hxk-3 were overexpressed after the treatment with *** successfully interfering hxk-1,hxk-2,and hxk-3,the 50%lethal concentration(LC50)of all mutant nematodes decreased with 3-BrPA treatment for 24 h compared with that of the *** the cyp35 genes tested were overexpressed,except *** induction of cyp-35A1 expression was most *** LC50 values of the mutant strains cyp-35A1,cyp-35A2,cyp-35A4,cyp-35B3,and cyp-35C1 were lower than that of the ***,the toxicity of 3-BrPA is closely related to its effect on hexokinase metabolism in nematodes,and the cyp-35 family plays a key role in the metabolism of 3-BrPA.

关键词： 3-Bromopyruvate Caenorhabditis elegans Hexokinase Cytochrome P450

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电针对溃疡性结肠炎大鼠结肠细胞自噬的影响

时珍国医国药 2024年第4期35卷 1022-1024,F0003,F0004页

作者：徐慧超王海军曹玉霞武荣林蒋子文李让钱冀来喜山西中医药大学第二临床学院山西晋中030619 山西中医药大学附属针灸推拿医院山西太原030006

目的探讨电针“肠病方”对溃疡性结肠炎(UC)大鼠结肠细胞自噬水平的调控作用。方法将40只雄性SD大鼠随机分为5组:正常对照组(正常组),UC模型组(模型组),电针治疗组(电针组),雷帕霉素(RAPA)溶液干预组(RAPA组),3-甲基腺嘌呤(3-ma)溶液干... 详细信息

目的探讨电针“肠病方”对溃疡性结肠炎(UC)大鼠结肠细胞自噬水平的调控作用。方法将40只雄性SD大鼠随机分为5组:正常对照组(正常组),UC模型组(模型组),电针治疗组(电针组),雷帕霉素(RAPA)溶液干预组(RAPA组),3-甲基腺嘌呤(3-ma)溶液干预组(3-ma组),每组8只大鼠。正常组大鼠给予正常饮食,其余4组大鼠采用5%葡聚糖硫酸钠(DSS)溶液建立UC大鼠模型。造模成功后,电针组大鼠采用电针刺激大鼠“中脘”及双侧“天枢”、“上巨虚”五个穴位,疏密波,频率10 Hz/50 Hz,强度以大鼠肢体轻微颤动,电针20 min。RAPA组大鼠腹腔注射2 mg/kg RAPA溶液,3-ma组大鼠腹腔注射1.5 mg/100 g 3-ma溶液。测量大鼠体质量、结肠长度,计算疾病活动指数(DAI)评分,并取各组大鼠血清及结肠组织,通过HE染色观察大鼠结肠形态学变化;酶联免疫吸附实验(ELISA)检测血清中肿瘤坏死因子(TNF-α)、白细胞介素(IL)-6、IL-2、IL-10含量;超微电镜观察大鼠结肠黏膜上皮细胞超微结构;Western blot法检测自噬相关蛋白(ATG)5、ATG 12、LC 3和p 62蛋白相对含量;实时荧光定量PCR(qPCR)检测ATG 5、ATG 12、LC 3和p 62 mRNA含量。结果与正常组相比,模型组、3-ma组大鼠DAI评分分值增加,结肠长度缩短,血清中IL-2、IL-10水平降低,TNF-α、IL-6水平增加,结肠组织中ATG 5、ATG 12、LC 3相对蛋白表达含量及该基因mRNA的相对含量显著降低,p 62相对蛋白表达含量及其基因mRNA的相对含量显著升高(Pma组大鼠DAI评分分值增加,结肠长度缩短,血清中IL-2、IL-10水平降低,TNF-α、IL-6水平升高,结肠组织中ATG 5、ATG 12、LC 3相对蛋白表达含量及该基因mRNA的相对含量显著降低,p 62相对蛋白表达含量及其基因mRNA的相对含量显著升高(P<0.05,P<0.01)。结论电针“肠病方”能够促进溃疡性结肠炎大鼠结肠细胞的自噬水平。

关键词：溃疡性结肠炎电针细胞自噬

轻质高强MXene/细菌纤维素复合气凝胶的制备及其电磁屏蔽性能

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复合材料学报 2023年第11期40卷 6407-6415页

作者：张艳马忠雷李桢景佳瑶邵亮陕西科技大学化学与化工学院陕西省轻化工助剂重点实验室西安710021 西北工业大学化学与化工学院陕西省高分子科学与技术重点实验室西安710129

随着5G通讯和可穿戴电子设备等高集成化和高功率化的快速发展,由电磁波引起的电磁干扰和电磁污染问题日益严重,亟需研发轻质高强且环境友好的电磁屏蔽复合材料。本文以生物质细菌纤维素(BC)为基体,导电Ti_(3)C_(2)T_(x)MXene为功能填料... 详细信息

随着5G通讯和可穿戴电子设备等高集成化和高功率化的快速发展,由电磁波引起的电磁干扰和电磁污染问题日益严重,亟需研发轻质高强且环境友好的电磁屏蔽复合材料。本文以生物质细菌纤维素(BC)为基体,导电Ti_(3)C_(2)T_(x)MXene为功能填料,通过液氮定向冷冻-冷冻干法制备轻质高强定向多孔结构MXene/BC复合气凝胶。深入研究了Ti_(3)C_(2)T_(x)MXene质量分数对复合气凝胶微观结构、导电性能、力学性能和电磁屏蔽性能的影响规律。结果表明:当Ti_(3)C_(2)T_(x)MXene质量分数为40wt%时,复合气凝胶的密度仅为18.3 mg/cm^(3),电导率和X波段电磁屏蔽效能(EMI SE)均达到最大,分别为459.3 S/cm和72 dB(厚度为4 mm)。由于BC和Ti_(3)C_(2)T_(x)MXene之间存在丰富的氢键相互作用,复合气凝胶在30%应变下压缩强度达到38.3 kPa,较纯BC气凝胶提升了116.1%。

关键词：电磁屏蔽复合气凝胶定向多孔结构细菌纤维素 Ti_(3)C_(2)T_(x)MXene

Neuroprotective mechanism of L-cysteine after subarachnoid hemorrhage

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Neural Regeneration Research 2020年第10期15卷 1920-1930页

作者： Ye xiong Dan-Qing xin Quan Hu Ling-xiao Wang Jie Qiu Hong-Tao Yuan xi-Li Chu De-xiang Liu Gang Li zhen Wang Department of Physiology School of Basic Medical SciencesShandong UniversityJinanShandong ProvinceChina Department of Neurosurgery Qilu Hospital of Shandong University and Brain Science Research InstituteShandong UniversityJinanShandong ProvinceChina Department of Neurosurgery Taian Central HospitalTaianShandong ProvinceChina Department of Medical Psychology and Ethics School of Basic Medicine SciencesShandong UniversityJinanShandong ProvinceChina

Hydrogen sulfide,which can be generated in the central nervous system from the sulfhydryl-containing amino acid,L-cysteine,by cystathionine-β-synthase,may exert protective effects in experimental subarachnoid hemorrhage;however,the mechanism underlying this effect is *** study explored the mechanism using a subarachnoid hemorrhage rat model induced by an endovascular perforation *** were treated with an intraperitoneal injection of 100 mM L-cysteine(30μL)30 minutes after subarachnoid *** 48 hours after subarachnoid hemorrhage,hematoxylin-eosin staining was used to detect changes in prefrontal cortex cells.L-cysteine significantly reduced cell *** function was assessed using a modified Garcia *** water content was measured by the wet-dry method.L-cysteine significantly reduced neurological deficits and cerebral edema after subarachnoid *** was used to detect the number of activated *** transcription-polymerase chain reaction(RT-PCR)was used to detect the levels of interleukin 1β and CD86 mRNA in the prefrontal cortex.L-cysteine inhibited microglial activation in the prefrontal cortex and reduced the mRNA levels of interleukin 1βand ***-PCR and western blot analysis of the complement system showed that L-cysteine reduced expression of the complement factors,C1q,C3αand its receptor C3aR1,and the deposition of C1q in the prefrontal *** staining was applied to detect changes in reactive oxygen species,and immunohistochemistry was used to detect the number of NRF2-and HO-1-positive cells.L-cysteine reduced the level of reactive oxygen species in the prefrontal cortex and the number of NRF2-and HO-1-positive *** blot assays and immunohistochemistry were used to detect the protein levels of CHOP and GRP78 in the prefrontal cortex and the number of CHOP-and GRP78-positive cells.L-cysteine reduced CHOP and GRP78 levels and the number of CHOP-and GRP78-

关键词： aminooxyacetic acid central nervous system complement deposition cystathionine-β-synthase early brain injury endoplasmic reticulum stress hydrogen sulfide neuroinflammation oxidative stress subarachnoid hemorrhage

CXCR4/SDF-1 axis is involved in lymph node metastasis of gastric carcinoma

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World Journal of Gastroenterology 2011年第19期17卷 2389-2396页

作者： Bao-Cheng Zhao zhen-Jun Wang Wei-zheng mao Hua-Chong ma Jia-Gang Han Bo Zhao Hui-Min Xu Department of General Surgery Beijing Chaoy- ang Hospital Capital Medical University Beijing 100020 China Department of General Surgery Qingdao Mu- nicipal Hospital Qingdao University Medical College Qingdao 266071 Shandong Province China Department of General Surgery Weifang People's Hospital Weifang 261041 Shangdong Province China

AIM:To investigate the role of CXC chemokine receptor-4 (CXCR4) and stromal cell-derived factor-1 (SDF-1) in lymph node metastasis of gastric ***:In 40 cases of gastric cancer,expression of CXCR4 mRNA in cancer and normal mucous membrane and SDF-1 mRNA in lymph nodes around the stomach was detected using quantitative polymerase chain reaction (PCR) (Taqman) and immunohistochemistric ***-7901 and MGC80-3 cancer cells were used to investigate the effect of SDF-1 on cell proliferation and ***:Quantitative reverse transcription PCR and immunohistochemistry revealed that the expression level of CXCR4 in gastric cancer was significantly higher than that in normal mucous membrane (1.6244 ± 1.3801 vs 1.0715 ± 0.5243,P mal nodes (0.5432 ± 0.4907 vs 0.2640 ± 0.2601,P man gastric cancer cell lines expressed CXCR4 and showed vigorous proliferation and migratory responses to ***3100 (a specific CXCR4 antagonist) was also found to effectively reduce the migration of gastric cancer ***:The CXCR4/SDF-1 axis is involved in the lymph node metastasis of gastric ***4 is considered as a potential therapeutic target in the treatment of gastric cancer.

关键词： Gastric carcinoma Chemokines Stromal cell-derived factor-1 CXC chemokine receptor-4 Lymph node metastasis

Relationship between HBV genotypes and anti-viral therapeutic efficacy of interferon-alpha

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Hepatobiliary & Pancreatic Diseases International 2007年第2期6卷 166-171页

作者： ma, Jin-Chun Wang, Lu-Wen Li, xin-Jian Liao, Yong-Feng Hu, xi-Ya Gong, Zuo-Jiong Wuhan Univ Renmin Hosp Dept Infect Dis State Key Lab Virol Wuhan 430060 Peoples R China Huangshi Aikang Hosp Dept Infect Dis Huangshi 435000 Peoples R China

BACKGROUND: Much evidence demonstrates that the genotypes of hepatitis B virus (HBV) present differences in pathogenicity and outcomes owing to differences in genetic structure. This study aimed to investigate the influences of HBV genotypes on the anti-viral therapeutic efficacy of interferon-alpha (IFN-alpha) in chronic hepatitis B patients, and to determine the relationship between HBV genotypes and levels of viral replication or gene variations. METHODS: The chronic hepatitis B patients who were treated with IFN-alpha were selected randomly. Anti-viral therapeutic efficacy was monitored in these patients. The HBV genotypes were detected by PCR microplate hybridization ELISA. The levels of serum HBV-DNA were determined by fluorescence quantitative PCR. HBV gene variation at pre-C and basic core promoter (BCP) regions were assayed by gene chip technology. RESULTS: Genotypes B and C were predominant in 94 chronic hepatitis B patients. A, E and F genotypes were not found in these patients. The HBV-DNA levels of genotype C and mixed genotypes were significantly higher than those of genotype B. The response to IFN-alpha in patients with genotype B was markedly better than in those with genotypes C and D, and the complete response to IFN-alpha was only observed in genotype B. The response to IFN-alpha in patients with mixed genotypes was the least sensitive. The negative transition of HBeAg was correlated with variations in the HBV pre-C and BCP regions in patients with partial or no response to IFN-alpha. The variation rates of HBV pre-C and BCP regions were clearly higher in genotype C than in genotype B. CONCLUSIONS: The results suggest that HBV genotype is correlated with the serum levels of HBV-DNA, HBV gene variations and therapeutic efficacy of IFN-alpha. The regular detection of HBV genotypes in the clinic will be of benefit for disease prognosis and planning of anti-viral therapeutic strategies.

关键词： hepatitis B virus genotype gene variation interferon-alpha anti-viral therapy

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钼及钼合金钎焊技术研究进展

云南冶金 2024年第4期53卷 109-113页

作者：魏亚馨操齐高杨晓红汪小钰雷征西安理工大学陕西西安710000 西北有色金属研究院陕西西安710000 西安市贵金属功能材料实验室陕西西安710000

钼及钼合金广泛应用于航空航天、核工业、新能源等多个领域,近年来受到国内外学者的广泛关注。本文综述了国内外钼及钼合金钎焊工艺研究现状以及钎焊材料的研究进展,并对钼及钼合金的钎焊技术发展趋势进行了展望。

关键词：钼合金硬脆性焊接性能钎焊钎料

Ultrasound augmenting injectable chemotaxis hydrogel for articular cartilage repair in osteoarthritis

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Chinese Chemical Letters 2021年第5期32卷 1759-1764页

作者： Hui Liu xi xiang Jianbo Huang Bihui Zhu Liyun Wang Yuanjiao Tang Fangxue Du Ling Li Feng Yan Lang ma Li Qiu Department of Ultrasound Laboratory of Ultrasound Imaging DrugWest China HospitalSichuan UniversityChengdu 610041China Department of Ultrasound The Affiliated Hospital of Southwest Medical UniversityLuzhou 646000China

The increasing incidence of osteoarthritis(OA) seriously affects life quality,posing a huge socioeco nomic *** engineering technology has become a hot topic in articular cartilage repair as one of the key treatment methods to alleviate ***,one of the most commonly used scaffold materials,ca n provide a good extracellular matrix microenvironment fo r seed cells such as bone marrow mesenchymal stem cells(BMSCs),which can promote cartilage ***,the low homing rate of stem cells severely limits their role in promoting articular cartilage *** mal cell-derived factor-1α(SDF-1α) plays a crucial role in the activatio n,mobilization,homing,and migration of *** rein,a novel injectable chemotaxis hydrogel,composed of chitosan-based injectable hydrogel and embedding SDF-1α-loaded nanodroplets(PFP@NDs-PEG-SDF-1α) was designed and *** ultrasound was then used to augment the injectable chemotaxis hydrogel and promote the homing migration of BMSCs for OA cartilage *** effect of ultrasound augmenting injectable PFP@NDs-PEG-SDF-1α/hydrogel on the migration of BMSCs was verified in vitro and in vivo,which re markably promotes stem cell homing and the repair of cartilage in the OA ***,the treatment strategy of ultrasound augmenting injectable chemotaxis hydrogel has a bright potential for OA articular cartilage repair.

关键词： SDF-la Injectable hydrogel Ultrasound Osteoarthritis Cartilage repair