Toxicity and metabolism of 3-bromopyruvate in Caenorhabditis elegans

作     者：Qiao-ling GU Yan ZHANG Xi-mei FU Zhao-lian LU Yao YU Gen CHEN Rong MA Wei KOU Yong-mei LAN Qiao-ling GU;Yan ZHANG;Xi-mei FU;Zhao-lian LU;Yao YU;Gen CHEN;Rong MA;Wei KOU;Yong-mei LAN

作者机构：The Institute of Minority Physique and HealthMedical College of Northwest University for NationalitiesLanzhou 730030China Department of AnesthesiologyFirst HospitalLanzhou UniversityLanzhou 730000China Pathogen Biology InstituteSchool of Basic Medical SciencesLanzhou UniversityLanzhou 730000China Department of MicrobiologySchool of Basic Medical SciencesGuilin Medical UniversityGuilin 541100China 

出 版 物：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 (浙江大学学报（英文版）B辑（生物医学与生物技术）)

年 卷 期：2020年第21卷第1期

页      面：77-86页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 100706[医学-药理学] 1001[医学-基础医学(可授医学、理学学位)] 0905[农学-畜牧学] 0906[农学-兽医学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Project supported by the National Natural Science Foundation of China(Nos.31172174 and 81460677) the Fundamental Research Funds for the Central Universities of China(No.31920170039) the Natural Science Found of Gansu Province(No.18JR3RA283),China 

主　　题：3-Bromopyruvate Caenorhabditis elegans Hexokinase Cytochrome P450 

摘      要：In this study,we aimed to evaluate the toxic effects,changes in life span,and expression of various metabolismrelated genes in Caenorhabditis elegans,using RNA interference(RNAi)and mutant strains,after 3-bromopyruvate(3-BrPA)*** was treated with various concentrations of 3-BrPA on nematode growth medium(NGM)plates,and their survival was monitored every 24 *** expression of genes related to metabolism was measured by the real-time fluorescent quantitative polymerase chain reaction(qPCR).Nematode survival in the presence of 3-BrPA was also studied after silencing three hexokinase(HK)*** average life span of *** cultured on NGM with 3-BrPA was shortened to 5.7 d compared with 7.7 d in the control ***-1,hxk-2,and hxk-3 were overexpressed after the treatment with *** successfully interfering hxk-1,hxk-2,and hxk-3,the 50%lethal concentration(LC50)of all mutant nematodes decreased with 3-BrPA treatment for 24 h compared with that of the *** the cyp35 genes tested were overexpressed,except *** induction of cyp-35A1 expression was most *** LC50 values of the mutant strains cyp-35A1,cyp-35A2,cyp-35A4,cyp-35B3,and cyp-35C1 were lower than that of the ***,the toxicity of 3-BrPA is closely related to its effect on hexokinase metabolism in nematodes,and the cyp-35 family plays a key role in the metabolism of 3-BrPA.