检索结果-南通市图书馆

Arabidopsis MSBP1 Is Activated by HY5 and HYH and Is Involved in Photomorphogenesis and Brassinosteroid Sensitivity Regulation

中国遗传学会植物遗传和基因组学专业委员会2009年学术研讨会

作者： li song,xiao-Yi Zhou,and hong-wei xue~* national key laboratory of plant molecular genetics,institute of plant physiology and ecology.shanghai institutes for biological sciences.chinese academy of sciences.300 fenglin road,shanghai 200032

Brassinosteroids(BRs) is crucial in regulating plant growth and development,as well as the photomorphogenesis,however,the mechanism,especially how they synergistically function with light and other factors,is still la... 详细信息

关键词： brassinosteroid photomorphogenesis hormone interplay ubquitin

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

molecular plant 2011年第6期4卷 1092-1104页

作者： Qiu-Ming Shi xi yang li song hong-wei xue national key laboratory of plant molecular genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 300 Fenglin Road 200032 Shanghai PR China

Membrane Steroid Binding Protein 1 （MSBP1） can bind steroids in vitro and negatively regulates brassinosteroid （BR） signaling, as well as cell elongation and expansion. Detailed analysis of the MSBP1 expression pattern based on quantitative real-time RT-PCR and promoter-GUS fusion studies revealed that MSBP1 expression in hypocotyls is stimulated by various light conditions, Interestingly, MSBP1 expression is greatly suppressed in hyS, hyh, or hy5hyh mutants but enhanced in cop1 mutants, Further analysis employing a yeast one-hybrid assay, an electrophoretic mobility shift assay （EMSA）, and a Chromatin IP （CHIP） assay confirmed the direct binding of Long Hypocotyl 5 （HY5） and HY5 Homolog （HYH） to the promoter region of MSBP1, indicating that MSBP1 is involved in light-regulated hypocotyl growth by serving as a direct target for HY5 and HYH. In addition, hy5 and hy5 hyh mutants show altered BR responses to light, which is consistent with the suppressed expression of MSBP1 in these mutants. These results suggest that light triggers MSBP1 ex- pression through direct binding to and activation by HY5 and HYH, thereby inhibiting hypocotyl elongation. The findings also provide informative clues regarding the mechanisms for the negative regulation of BR sensitivity and photomorpho- genesis during the dark-light transition.

关键词： MSBP1 HY5 HYH photomorphogenesis BR signaling.

Genome-Wide Analysis Regulatory Network of Photomorphogenesis Revealed the Complex Brassinosteroid Effects in

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

molecular plant 2009年第4期2卷 755-772页

作者： li song xiao-Yi Zhou li li liang-Jiao xue xi yang hong-wei xue national key laboratory of plant molecular genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 300 Fenglin Road 200032 Shanghai China

light and brassinosteroids （BRs） have been proved to be crucial in regulating plant growth and development; however, the mechanism of how they synergistically function is still largely unknown. To explore the underlying mechanisms in photomorphogenesis, genome-wide analyses were carried out through examining the gene expressions of the dark-grown WT or BR biosynthesis-defective mutant det2 seedlings in the presence of light stimuli or exogenous Brassinolide （BL）. Results showed that BR deficiency stimulates, while BL treatment suppresses, the expressions of lightresponsive genes and photomorphogenesis, confirming the negative effects of BR in photomorphogenesis. This is consistent with the specific effects of BR on the expression of genes involved in cell wall modification, cellular metabolism and energy utilization during dark-light transition. Further analysis revealed that hormone biosynthesis and signaling-related genes, especially those of auxin, were altered under BL treatment or light stimuli, indicating that BR may modulate photomorphogenesis through synergetic regulation with other hormones. Additionally, suppressed ubiquitin-cycle pathway during light-dark transition hinted the presence of a complicated network among light, hormone, and protein degradation. The study provides the direct evidence of BR effects in photomorphogenesis and identified the genes involved in BR and light signaling pathway, which will help to elucidate the molecular mechanism of plant photomorphogenesis.

关键词： brassinosteroid light photomorphogenesis hormone interplay ubiquitin.

Membrane Steroid Binding Protein 1 （MSBP1）Stimulates Tropism by Trafficking and Auxin Regulating Vesicle Redistribution

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

molecular plant 2008年第6期1卷 1077-1087页

作者： xi yang li song hong-wei xue national key laboratory of plant molecular genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy ofSciences 300 Fenglin Road 200032 Shanghai China

Overexpression of membrane steroid binding protein 1 （MSBP1） stimulates the root gravitropism and antigravitropism of hypocotyl, which is mainly due to the enhanced auxin redistribution in the bending regions of hypocotyls and root tips. The inhibitory effects by 1-N-naphthylphthalamic acid （NPA）, an inhibitor of polar auxin transport, are suppressed under the MSBP1 overexpression, suggesting the positive effects of MSBP1 on polar auxin transport. Interestingly, sub-cellular localization studies showed that MSBP1 is also localized in endosomes and observations of the membraneselective dye FM4-64 revealed the enhanced vesicle trafficking under MSBP1 overexpression. MSBPl-overexpressing seedlings are less sensitive to brefeldin A （BFA） treatment, whereas the vesicle trafficking was evidently reduced by suppressed MSBP1 expression. Enhanced MSBP1 does not affect the polar localization of PIN2, but stimulates the PIN2 cycling and enhances the asymmetric PIN2 redistribution under gravi-stimulation. These results suggest that MSBP1 could enhance the cycling of PIN2-containing vesicles to stimulate the auxin redistribution under gravi-stimulation, providing informative hints on interactions between auxin and steroid binding protein.

关键词： MSBP1 polar auxin transport auxin response gravitropism vesicle trafficking.

Brassinosteroids Regulate the Differential Growth of Arabidopsis Hypocotyls through Auxin Signaling Components IAA19 and ARF7

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

molecular plant 2013年第3期6卷 887-904页

作者： xiao-Yi Zhou li song hong-wei xue national key laboratory of plant molecular genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 300 Fenglin Road 200032 Shanghai China

Brassinosteroids （BRs） are an important class of phytohormones which regulates a wide range of physiological processes. Genetic and physiological studies have revealed that BR responses usually depend on an intact auxin signaling pathway. Here, we demonstrate that high BR concentration or enhanced BR signaling induce the differential growth of etiolated hypocotyls and result in the morphological changes, while auxin-resistant mutants, msg2 （dominant mutant of IAA19） and arf7, are insensitive to the BR effect and can partially suppress the phenotype of bzrl-D （dominant mutant of BZR1 with enhanced BR signaling）. Interestingly, BZR1 protein can directly bind to the promoter regions of both IAA19 and ARFT, indicating that IAA19 and ARF7 mediate the BR-induced differential growth by serving as direct targets of BZR1. Systemic microarray analysis revealed that a number of BR-responsive genes showed reduced BR response in msg2, confirming that BR employs auxin signaling components IAA19 and ARF7 to modulate the specific downstream processes. These results provide informative clues on the crosstalk of BR-auxin signaling and the mechanisms of BR-auxin effects in regulating differential growth.

关键词： brassinosteroids (BRs) auxin IAA19 ARF7 differential growth Arabidopsis.

Tumor-derived neomorphic mutations in ASXL1 impairs the BAP1-ASXL1-FOXK1/K2 transcription network

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Protein & Cell 2021年第7期12卷 557-577页

作者： Yu-Kun xia Yi-Rong Zeng Meng-li Zhang Peng liu Fang liu Hao Zhang Chen-xi He Yi-Ping Sun Jin-Ye Zhang Cheng Zhang Lei song Chen Ding Yu-Jie Tang Zhen yang Chen yang Pu Wang Kun-liang Guan Yue xiong Dan Ye Huashan Hospital Fudan Universityand Molecular and Cell Biology LabInstitutes of Biomedical Sciencesand the Shanghai Key Laboratory of Medical Epigeneticsand the Key Laboratory of Metabolism and MolecularMinistry of EducationShanghai 200032China The International Co-laboratory of Medical Epigenetics and Metabolism Ministry of Science and TechnologyShanghai 200032China Department of Neurosurgery Huashan HospitalFudan UniversityShanghai 200032China key laboratory of Cell Differentiation and Apoptosis of national Ministry of Education Department of PathophysiologyShanghai Jiao Tong University School of MedicineShanghai 200025China key laboratory of Synthetic Biology Institute of Plant Physiology and EcologyShanghai 200032China institutes for biological sciences Chinese Academy of SciencesShanghai 200032China State key laboratory of Proteomics Beijing Proteome Research CenterBeijing Institute of Radiation MedicineBeijing 102206China national Center for Protein sciences.The PHOENIX Center Beijing)Beijing 102206China State key laboratory of Genetic Engineering and Collaborative Innovation Center for genetics and Development School of Life SciencesInstitutes of Biomedical SciencesFudan UniversityShanghai 200032China Department of Pharmacology and Moores Cancer Center University of California San DiegoLa JollaCAUSA lineberger Comprehensive Cancer Center Department of Biochemistry and BiophysicsUniversity of North CarolinaChapel HillNCUSA Department of General Surgery Huashan HospitalFudan UniversityShanghai 200032China

Additional sex combs-like 1(ASXL1)interacts with BRCA1-associated protein 1(BAP1)deubiquitinase to oppose the polycomb repressive complex 1(PRC1)-mediated histone H2A *** BAP1 mutations are found in a spectrum of human malignancies,while ASXL1 mutations recurrently occur in myeloid neoplasm and are associated with poor *** all ASXL1 mutations are heterozygous frameshift or nonsense mutations in the middle or to a less extent the C-terminal region,resulting in the production of C-terminally truncated mutant ASXL1 *** ASXL1 regulates specific target genes and how the C-terminal truncation of ASXL1 promotes leukemogen-esis are ***,we report that ASXL1 interacts with forkhead transcription factors FOXK1 and FOXK2 to regulate a subset of FOXK1/K2 target *** show that the C-terminally truncated mutant ASXL1 proteins are expressed at much higher levels than the wild-type protein in ASXL1 heterozygous leukemia cells,and lose the ability to interact with FOXK1/*** deletion of the mutant allele eliminates the expression of C-termi-nally truncated ASXL1 and increases the association of wild-type ASXL1 with BAP1,thereby restoring the expression of BAP1-ASXL1-FOXK1/K2 target genes,particularly those involved in glucose metabolism,oxygen sensing,and JAK-STAT3 signaling *** addition to FOXK1/K2,we also identify other DNA-bind-ing transcription regulators including transcription factors(TFs)which interact with wild-type ASXL1,but not C-terminally truncated *** results suggest that ASXL1 mutations result in neomorphic alleles that contribute to leukemogenesis at least in part through dominantly inhibiting the wild-type ASXL1 from interacting with BAP1 and thereby impairing the function of ASXL1-BAP1-TF in regulating target genes and leukemia cell growth.

关键词： ASXL1 BAP1 FOXK1/K2 leukemia epigenetics

On the origin of SARS-CoV-2——The blind watchmaker argument

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Science china(life sciences. 2021年第9期64卷 1560-1563页

作者： Chung-I Wu Haijun Wen Jian Lu xiao-dong Su Alice CHughes weiwei Zhai Chen Chen Hua Chen Mingkun li Shuhui song Zhaohui Qian Qihui Wang Bingjie Chen Zixiao Guo Yongsen Ruan xuemei Lu Fuwen wei li Jin Le Kang Yongbiao xue Guoping Zhao Ya-Ping Zhang State key laboratory of Biocontrol School of Life SciencesSun Yat-sen UniversityGuangzhou 510275China State key laboratory of Protein and plant Gene Research Center for BioinformaticsSchool of Life SciencesPeking UniversityBeijing 100871China Landscape ecology.Group Center for Integrative ConservationXishuangbanna Tropical Botanical GardenChinese Academy of SciencesMengla 666303China key laboratory of Zoological Systematics and Evolution Institute of ZoologyChinese Academy of SciencesBeijing 100101China Biomedical Innovation Center Beijing Shijitan HospitalCapital Medical UniversityBeijing 100038China Beijing institute of Genomics Chinese Academy of Sciencesand China National Centre for BioinformationBeijing 100101China institute of Pathogen Biology Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100176China CAS key laboratory of Pathogen Microbiology and Immunology Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China State key laboratory of Genetic Resources and Evolution Kunming Institute of ZoologyChinese Academy of SciencesKunming 650223China CAS key laboratory of Animal ecology.and Conservation Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China MOE key laboratory of Contemporary Anthropology Department of Anthropology and Human GeneticsSchool of Life SciencesFudan UniversityShanghai 200433China State key laboratory of Integrated Management of Pest Insects and Rodents Institute of ZoologyChinese Academy of SciencesBeijing 100101China key laboratory of Synthetic Biology Institute of Plant Physiology and EcologyShanghai Institutes for Biological SciencesChinese Academy of SciencesShanghai 200032China

In the comparison with SARS-CoVof 2003,SARS-CoV-2 is extremely well adapted to the human populations and its adaptive shift from the animal host to humans must have been even more *** the blind watchmaker argument,such an adaptive shift can only happen prior to the onset of the current pandemic and with the aid of step-by-step selection.

关键词： argument origin adapted