Arabidopsis MSBP1 Is Activated by HY5 and HYH and Is Involved in Photomorphogenesis and Brassinosteroid Sensitivity Regulation
Arabidopsis MSBP1 Is Activated by HY5 and HYH and Is Involved in Photomorphogenesis and Brassinosteroid Sensitivity Regulation作者机构：National Key Laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 300 Fenglin Road 200032 Shanghai PR China
出 版 物：《Molecular Plant》 (分子植物（英文版）)
年 卷 期：2011年第4卷第6期
页 面：1092-1104页
核心收录：
学科分类：0710[理学-生物学] 090403[农学-农药学(可授农学、理学学位）] 07[理学] 071009[理学-细胞生物学] 09[农学] 0904[农学-植物保护] 0901[农学-作物学] 090102[农学-作物遗传育种]
基 金：National Science Foundation [90717001 30721061]
主 题：MSBP1 HY5 HYH photomorphogenesis BR signaling.
摘 要：Membrane Steroid Binding Protein 1 （MSBP1） can bind steroids in vitro and negatively regulates brassinosteroid （BR） signaling, as well as cell elongation and expansion. Detailed analysis of the MSBP1 expression pattern based on quantitative real-time RT-PCR and promoter-GUS fusion studies revealed that MSBP1 expression in hypocotyls is stimulated by various light conditions, Interestingly, MSBP1 expression is greatly suppressed in hyS, hyh, or hy5hyh mutants but enhanced in cop1 mutants, Further analysis employing a yeast one-hybrid assay, an electrophoretic mobility shift assay （EMSA）, and a Chromatin IP （CHIP） assay confirmed the direct binding of Long Hypocotyl 5 （HY5） and HY5 Homolog （HYH） to the promoter region of MSBP1, indicating that MSBP1 is involved in light-regulated hypocotyl growth by serving as a direct target for HY5 and HYH. In addition, hy5 and hy5 hyh mutants show altered BR responses to light, which is consistent with the suppressed expression of MSBP1 in these mutants. These results suggest that light triggers MSBP1 ex- pression through direct binding to and activation by HY5 and HYH, thereby inhibiting hypocotyl elongation. The findings also provide informative clues regarding the mechanisms for the negative regulation of BR sensitivity and photomorpho- genesis during the dark-light transition.
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