检索结果-南通市图书馆

A mitochondria-targeted H_(2)S-activatable fluorogenic probe for tracking hepatic ischemia-reperfusion injury

Chinese Chemical Letters 2024年第6期35卷 370-375页

作者： Bin Fang Jiaqi Yang Limin Wang Haoqin Li Jiaying Guo Jiaxin Zhang Qingyuan Guo Bo Peng Kedi Liu miaomiao xi Hua Bai Li Fu Lin Li School of Materials Science and Engineering Northwestern Polytechnical UniversityXi’an 710072China Frontiers Science Center for Flexible Electronics Xi’an Institute of Flexible Electronics(IFE)and Xi’an Institute of Biomedical Materials&EngineeringNorthwestern Polytechnical UniversityXi’an 710072China Key laboratory of Flexible Electronics of Zhejiang Province Ningbo Institute of Northwestern Polytechnical UniversityNingbo 315103China The Institute of Flexible Electronics(IFE Future Technologies)Xiamen UniversityXiamen 361005China Wuhan National Laboratory for Optoelectronics-Advanced Biomedical Imaging Facility Huazhong University of Science and TechnologyWuhan 430074China TANK Medicinal Biology Institute of xi’an Xi’an 710032China

Hepatic ischemia-reperfusion injury(HIRI)is the cause of postoperative hepatic dysfunction and failure,and even *** an important biological effector molecule,hydrogen sulfide(H_(2)S)of mitochondria as a gasotransmitter that is usually used to protect against acute HIRI ***,the exact relationship between HIRI and mitochondrial H_(2)S remains tangled due to the lack of an effective analytical ***,we have fabricated a mitochondria-targeted H_(2)S-activatable fluorogenic probe(Mito-GW)to explore the stability of mitochondrial H_(2)S and track the changes of mitochondrial H_(2)S during the *** virtue of pyridinium electropositivity and its amphiphilicity,Mito-GW could accumulate in *** goes through an analyte-prompted immolation when reacts with H_(2)S,resulting in the releasing of the fluorophore(GW).Therefore,the extent of Mito-GW conversion to GW can be used to evaluate the changes of mitochondrial H_(2)S level in living cells and *** proof-of-principle,we have used MitoGW to demonstrate the mitochondria H_(2)S-levels increase and then decrease during HIRI in vitro and in *** research highlights the tremendous potential of Mito-GW as a mitochondrial H_(2)S fluorogenic probe in elucidating the pathogenesis of HIRI,providing a powerful tool for promoting future research on hepatology.

关键词： Mitochondria-targeted Bioimaging H2S-activatable Fluorogenic probe Hepatic ischemia-reperfusion injury(HIRI)

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

高内涵筛选技术在中药作用机制及物质基础研究中的应用进展

引用

中药药理与临床 2024年第4期40卷 121-128页

作者：李星雨王婧陈思凯高峰欧莉李敏奚苗苗董泰玮卫培峰陕西中医药大学咸阳712046 陕西中医药大学第二附属医院咸阳712021 西安天工生物医药研究所西安710032

中药的现代化研究一直存在药效物质基础筛选复杂且筛选不全面,作用机制研究靶点多且认识不深入的问题,致使其研究结果存在不确定性及不充分性,无法得到国际认可。而高内涵筛选技术(High-content screening,HCS)又称高内涵分析技术(High-... 详细信息

中药的现代化研究一直存在药效物质基础筛选复杂且筛选不全面,作用机制研究靶点多且认识不深入的问题,致使其研究结果存在不确定性及不充分性,无法得到国际认可。而高内涵筛选技术(High-content screening,HCS)又称高内涵分析技术(High-content analysis,HCA),作为一项高通量、多靶点、多通道、全自动化的分析技术,能够量化分析大量细胞对药物的特异性反应图像,可在中药的现代化研究中以匹配中药及其复方多靶点作用的应用特性,对其进行作用机制及物质基础研究,以提高中药疗效、扩大用药范围、保障用药安全并使其结果具有相当的国际认可度。文章简要介绍了高内涵筛选的原理及其在中药作用机制及物质基础研究中的应用进展,从实例中,思考其优势与限制及对中药现代化研究做出的贡献。

关键词：高内涵分析技术高内涵筛选技术中药物质基础作用机制信号采集安全性评价

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

基于生态瞬时评估的男男性行为者高危性行为影响因素分析

引用

中国艾滋病性病 2024年第9期30卷 924-929页

作者：陈珍席苗苗张泽文吕朝康署丽盼·阿斯力别克戴江红新疆医科大学公共卫生学院乌鲁木齐830011 浙江省湖州市菱湖人民医院浙江湖州313000

目的基于生态瞬时评估技术,探讨MSM高危性行为的危险因素。方法依托乌鲁木齐市社会组织,基于网络招募MSM,基线调查收集一般人口学特征及近14天性行为信息,使用生态瞬时评估法连续14天调查,基于“经验抽样”在一天内两次收集MSM性行为信... 详细信息

目的基于生态瞬时评估技术,探讨MSM高危性行为的危险因素。方法依托乌鲁木齐市社会组织,基于网络招募MSM,基线调查收集一般人口学特征及近14天性行为信息,使用生态瞬时评估法连续14天调查,基于“经验抽样”在一天内两次收集MSM性行为信息、毒品(精神活性物质)使用情况和“每日日记”调查方法收集调查对象过去24 h内的性行为、毒品使用情况以及计划性行为情况。采用广义估计方程模型分析乌鲁木齐市MSM发生高危性行为的影响因素。结果共纳入156名MSM,平均年龄(33.61±8.88)岁。基线调查中,MSM无套肛交(无保护肛交)发生率为17.3%(24人)。广义估计方程多因素结果显示,在经验抽样调查中,使用毒品(OR=4.084,95%CI:1.820~9.167)、性伴服用PrEP(OR=3.302,95%CI:1.139~9.572)是MSM发生高危性行为的危险因素。在每日日记调查中,服用PrEP(OR=6.400,95%CI:1.689~24.249)、使用毒品(4.404,95%CI:1.234~15.716)以及安全套使用意图(OR=6.684,95%CI:1.565~30.093)是MSM发生高危性行为的影响因素。结论生态瞬时评估调查显示MSM发生无套肛交性行为仍有一定比例,安全套的使用今后仍然是MSM社区HIV干预中的一项重要策略,尤其针对有计划发生性行为的MSM亚群。

关键词：男男性行为者高危性行为生态瞬时评估

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

乌鲁木齐市男男同性性行为人群人乳头瘤病毒队列失访及影响因素研究

引用

中国预防医学杂志 2024年第6期25卷 650-656页

作者：张泽文刘礼荣席苗苗凌曦张国珍戴江红新疆医科大学公共卫生学院新疆维吾尔自治区乌鲁木齐830011

目的了解新疆维吾尔自治区乌鲁木齐市男男同性性行为人群(men who have sex with men,MSM)人乳头瘤病毒(human papilloma virus,HPV)队列失访发生情况,探讨失访发生的相关影响因素。方法2017年3月—2021年6月,本研究基于乌鲁木齐市社会... 详细信息

目的了解新疆维吾尔自治区乌鲁木齐市男男同性性行为人群(men who have sex with men,MSM)人乳头瘤病毒(human papilloma virus,HPV)队列失访发生情况,探讨失访发生的相关影响因素。方法2017年3月—2021年6月,本研究基于乌鲁木齐市社会组织招募MSM建立动态队列,每6个月随访1次,基线和随访均进行问卷调查,使用棉拭子采集肛周脱落细胞检测HPV DNA;利用Cox回归模型探讨失访发生的影响因素。结果本研究纳入MSM 822名,随访1433人年,总失访发生率为42.31/100人年。随访第1年失访发生率最高,为53.05(47.36~59.18)/100人年,随访第5年失访发生率最低,为7.04(2.19~16.36)/100人年。多因素分析显示,年龄>30岁[风险比(hazard ration,HR)=0.60,95%置信区间(confidence interval,CI):0.41~0.89]和乌鲁木齐市户口(HR=0.74,95%CI:0.53~1.04)的MSM不易失访;近1年性伙伴性别男女均有者(HR=0.23,95%CI:0.06~0.98)未做过HIV咨询检测(HR=1.90,95%CI:1.22~2.94)是失访发生的危险因素;与近6个月有肛交性行为者(HR=0.50,95%CI:0.25~0.99),有2~4个性伴的MSM更不易失访(HR=0.45,95%CI:0.21~0.97)。结论乌鲁木齐市MSM队列失访发生率较高,今后可通过加强新入组研究对象宣传教育,强化与研究对象间的信任关系,考虑MSM行为特征与参与研究动机之间的联系,重新评估队列保留策略,加强随访管理,提高随访率。

关键词：男男同性性行为人群人乳头瘤病毒失访

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

中药引经干预脑病的研究进展

引用

中华中医药杂志 2024年第9期39卷 4815-4820页

作者：连佳宁马艺伦皇冯玉张萌萌李瑶李敏卫培峰奚苗苗唐于平董泰玮陕西中医药大学药学院咸阳712046 陕西中医药大学第二附属医院咸阳712046

近年脑部疾病发病率升高,严重威胁人类健康。引经药是中医用药的一大特色,其引导药物直达病所或进入特定脏腑经络的特性在治疗脑病时具有独特优势。文章对中药引经干预脑病的相关文献及古籍记载进行整理概括,发现引经药能够促进其他药... 详细信息

近年脑部疾病发病率升高,严重威胁人类健康。引经药是中医用药的一大特色,其引导药物直达病所或进入特定脏腑经络的特性在治疗脑病时具有独特优势。文章对中药引经干预脑病的相关文献及古籍记载进行整理概括,发现引经药能够促进其他药物或成分跨血脑屏障入脑,在中药复方及其制剂中发挥入脑导向作用,某些从中药中提取的活性成分还能够修饰药物载体,增强载体脑靶向性,对于中药在脑部疾病中的临床应用具有指导意义。

关键词：引经理论载体冰片脑病脑卒中阿尔茨海默病脑胶质瘤癫痫

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Antioxidant properties of magnesium lithospermate B contribute to the cardioprotection against myocardial ischemia/reperfusion injury in vivo and in vitro

引用

Journal of Traditional Chinese Medicine 2013年第1期33卷 85-91页

作者： Wei Quan Ying Yin miaomiao xi Dan Zhou Yanrong Zhu Yue Guan Chao Guo Yanhua Wang Jialin Duan Aidong Wen Department of Pharmacy Xijing HospitalFourth Military Medical University

OBJECTIVE： To determine the cardioprotective ef- fect of magnesium lithospermate B （MLB） on myo- cardial ischemia/reperfusion （MI/R） injury and to in- vestigate the antioxidant potential in vivo and in vitro. METHODS： MI/R injury was induced by the occlu- sion of left anterior descending coronary artery for 30 min followed by reperfusion for 3 h in rats. After reperfusion, hearts were harvested to assess infarct size, histopathological damages, the levels of superoxide dismutase （SOD）, catalase （CAT）, glutathione peroxidase （GPx）, reduced glutathione （GSH） and malondialdehyde （MDA）. Blood samples were collected to determine serum levels of creatine kinase-MB （CK-MB）, cardiac troponin （cTnl） and lactate dehydrogenase （LDH）. Furthermore, simulatedischemia/reperfusion （SI/R） injury in vitro was established by oxygen and glucose deprivation （OGD） for 2 h followed by 24-hour recovery period in cardiomyocytes. The activity of LDH in the cultured su- pernatant and the levels of intracellular reactive oxygen species （ROS）, SOD and MDA in cardiomyo- cytes were also measured. Finally, cardiomyocytes apoptosis was determined with flow cytometry. RESULTS： MLB significantly limited infarct size, ameliorated histopathological damages and prevented leakage of CK-MB, cTnl and LDH. Additional- ly, SOD, CAT, GPx and GSH activities were notably increased by MLB, along with the MDA content decreased as compared with the model group in rats. In vitro study, MLB also decreased LDH activity in the cultured supernatant, increased SOD activity in cardiomyocytes, reduced intracellular ROS and MDA levels, and significantly suppressed cardiomyocytes apoptosis. CONCLUSION： MLB possessed remarkably cardioprotective effects on MI/R injury in vivo and in vitro. The protection of MLB may contribute to its antioxidant properties.

关键词： Ischemia Reperfusion injury Myocytes, Cardiac Oxidative stress Antioxidants In vitro Magnesium lithospermate B

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Three new dimers and two monomers of phenolic acid amides from the fruits of Lyciumbarbarum and their antioxidant activities

Three new dimers and two monomers of phenolic acid amides fr...

引用

全国第二届海洋与陆地多糖多肽及天然创新药物研发学术会议

作者： TANG Haifeng GAO Kai MA Dongwei xi miaomiao Institute of Materia Medica School of PharmacyFourth Military Medical University Department of Pharmacy Xijing HospitalFourth Military Medical University Shaanxi Ark Pharmaceutical Co.Ltd.

Three new dimers of phenolic amides,named lyciumamides A(3),B(4)and C(5),along with two known monomers,N-?-coumaroyl tyramine(1)and N-?-feruloyl tyramine(2),were isolated from the fruits of Lyciumbarbarum for the first time,with help of the activity-guided *** amides were also firstly found in the genus *** structures were determined using spectral analysis and comparing with literature *** 1-5 were evaluated for their antioxidant activities in scavenging DPPH free radical and inhibiting lipid peroxidation in rat liver microsomes induced by ascorbate/Fe,cumine hydroperoxide or CCl/reduced form of nicotinamide-adenine dinucleotide phosphate,and the results showed that all of them exhibited strong activities while compounds 1 and 2 were more potent than the reference *** data supported that these phenolic amides played an important role on the natural antioxidant properties of the fruits of ***,similar to other phenolic constituents such as flavonoids and phenolic acids of the fruits.1 In conclusion,the results complemented the current knowledge of the antioxidative composition of the fruits of *** and confirmed that this agricultural product contained phenolic amide compounds,which might offer interesting potentialapplications in the food,cosmetic,and pharmaceutical industries.

关键词： Lyciumbarbarum Phenolic acid amides Lyciumamide N-E-coumaroyl tyramine N-E-feruloyl tyramine Antioxidant activities

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Salvianolic acid B regulates macrophage polarization in ischemic/reperfused hearts by inhibiting mTORC1-induced glycolysis

Salvianolic acid B regulates macrophage polarization in isch...

引用

2019中国生理学会学术年会暨张锡钧基金第十五届全国青年优秀生理学学术论文交流会及第十三届全国青年生理学工作者学术会议

作者：赵美娜 miaomiao xi Aidong Wen Jingwen Wang Department of Pharmacy Xijing HospitalFourth Military Medical University

Macrophages play important roles in the healing and remodeling of cardiac tissues after myocardial ischemia/reperfusion(MI/R) *** we investigated the potential effects of salvianolic acid B(SalB), one of the abundant and bioactive compounds extracted from Chinese herb Salvia Miltiorrhiza(Danshen), on macrophage-mediated inflammation after MI/R and the underlying *** primary cultured bone marrow-derived macrophages(BMDMs), SalB attenuated lipopolysaccharide(LPS)-induced M1 biomarkers(IL-6, iNOS, CCL2 and TNF-α) mRNA expression in a concentration-dependent *** contrast, M2 biomarkers(Arg1, Clec10 a and Mrc) mRNA levels following interleukinin-4(IL-4) stimulation were significantly upregulated by *** addition, LPS stimulation potently induced transcriptional upregulation of RagD, an important activation factor of mammalian target of rapamycin complex 1(mTORC1).Interestingly,SalB inhibited RagD upregulation and mTORC1 activation, decreased glycolysis, and reduced inflammatory cytokine production in LPS-stimulated macrophages,all of which were blunted in RagD knockdown *** mice subjected to MI/R, SalB treatment decreased cardiac M1-macrophages and increased M2-macrophages at 3 days post-MI/R, followed by decreased collagen deposition and ameliorated cardiac dysfunction at 7 days post-MI/***, our data have shown that SalB decreases M1-polarized macrophages in MI/R hearts via inhibiting mTORC1-dependent glycolysis, which might contribute to alleviated inflammation and improved cardiac dysfunction afforded by SalB after MI/R.

关键词： Macrophage polarization Myocardial ischemia/reperfusion injury Salvianolic acid B MTORC1

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Therapeutic Time Window and Underlying Therapeutic Mechanism of Breviscapinun Injection against Cerebral Ischemia/Reperfusion Injury in Rats

Therapeutic Time Window and Underlying Therapeutic Mechanism...

引用

第十五届中国科协年会第21分会场：中药与天然药物现代研究学术研讨会

作者： GUO Chao ZHU Yanrong QUAN Wei YIN Ying xi miaomiao ZHOU Dan WEN Aidong Fourth Military Medical University

Our study was designed to investigate the therapeutic time window and underlying therapeutic mechanism of breviscapinun injection against cerebral ischemic / reperfusion injury. In the present study, Sprague-Dawley rats were subjected to middle cerebral artery occlusion for 2 h. Rats were intravenous injected with 40 mg/kg breviscapinun injection at different time-points and the same doses were administered every 24 h for 3 consecutive days. After 72 h of reperfusion, neurologic scores, infarct volume and the levels of neuron-specific enolase were measured in a masked fashion. To explore the role of HIF-1α in the neuroprotective effects of breviscapinun injection, immunohistochemistry and western blot analysis in a separate study were performed after 3 and 24 h of reperfusion. Treatment with breviscapinun significantly ameliorated neurologic deficit, reduced infarct volume and suppressed the NSE levels in a time-dependent manner. Moreover, treatment with breviscapinun increased the expression of HIF-1α protein at 3 and 24 h after reperfusion. These results suggest that the therapeutic time window of breviscapinun injection for cerebral ischemia / reperfusion injury seem to be within 5 h after reperfusion, and by increasing the expression of HIF-1α may be involved in the therapeutic mechanism of breviscapinun.

关键词： breviscapinun ischemia/reperfusion therapeutic time window hypoxia-inducible factor-1

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

The serum metabolomic study of intervention effects of the traditional Chinese medicine Radix salviae miltiorrhizae and Lignum dalbergiae odoriferae in the treatment of myocardial ischemia/reperfusion injury in rats

The serum metabolomic study of intervention effects of the t...

引用

2016年中国药学大会暨第十六届中国药师周

作者： FEI MU JIALIN DUAN HAIXU BIAN RUI LIN MEINA ZHAO AIDONG WEN YONG YANG miaomiao xi Department of Pharmacy Xijing HospitalFourth Military Medical University Department of Pharmacy Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital

. Radix salviae miltiorrhizae（SM） and Lignum dalbergiae odoriferae（DO） are both traditional Chinese medicinal herbs for the treatment of ischemic heart disease and other cardiovascular diseases, but few of literature is about the systematic studies. Sprague-Dawley rats were pretreated with SM, aqueous extract of DO（DOA） and volatile oil of DO（DOO）, either as monotherapy or in combination for 7 days, then subjected to 30 min of ischemia followed by 180 min of reperfusion. Traditional pharmacodynamic evaluation and metabonomics based on gas chromatography/time-of-flight mass spectrometry（GC/TOF-MS） were used to identify the therapeutic effect of these traditional Chinese medicines. The results showed that SM, DOA and DOO ameliorated cardiac function respectively, and this effect was further strengthened by their combinations. Among all combinations, SM+DOO showed predominant potential（P＜0.01） to improve electrocardiogram and heart rate, reduce heart weight index and myocardial infarct size, decrease the levels of CK-MB and LDH. Moreover, metabonomics-based findings such as the principal component analysis（PCA） and partial least squares discriminant analysis（PLS-DA） score plot of metabolic state in rat serum correlated well to the preceding results, confirming that SM+DOO exerted synergistic therapeutic efficacies to exhibit better effect on MI/R injury rats compared with other pretreatment groups. Furthermore, the maximum effective point of SM+DOO was 30 min and the minimum effective point was 180 min. In addition, SM+DOO group markedly（P＜0.01） reduced the number of TUNEL-positive cells, the levels of TNF-α, IL-6 and MDA, while increased the activity of SOD both in serum and tissue. These results indicated that SM+DOO have combined effects that are highly effective than single pretreatment against MI/R injury rats. This effect might be achieved partly through anti-apoptosis, anti-oxidant and anti-inflammatory. Thus SM+DOO might be an effective and promising medicine f

关键词： Myocardial ischemia/reperfusion injury Radix salviae miltiorrhizae Lignum dalbergiae odoriferae The maximum and minimum effective points Cardioprotection Metabonomics

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：