检索结果-南通市图书馆

Effect of mitochondrial K_(ATP) channel on voltage gated K^+ channel in 24 hour-hypoxic human pulmonary artery smooth muscle cells

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Journal 2005年第1期118卷 12-19页

作者： WANGTao ZHANGZhen-xiang xuyong-jian DepartmentofRespiratoryMedicine TongjiHospitalTongjiMedicalCollegeHuazhongUniversityofScienceandTechnologyWuhan430030China DepartmentofRespiratoryMedicine TongjiHospitalTongjiMedicalCollegeHuazhongUniversityofScienceandTechnologyWuhan430030China

Background Hypoxic pulmonary hypertension (HPH) is initiated by inhibition of O 2 sensitive, voltage gated (Kv) channels in pulmonary arterial smooth muscle cells (PASMCs) The mechanism of hypoxic pulmonary hypertension has not yet been fully elucidated The mitochondrial ATP sensitive K + channel (MitoK ATP ) is extremely sensitive to hypoxia, and is a decisive factor in the control of mitochondrial membrane potential (ΔΨ m) This study investigated the changes of cell membrane potential and Kv channel in cultured human pulmonary artery smooth muscle cell (hPASMC) exposed to 24 hour hypoxia, and explored the role of MitoK ATP and ΔΨ m in this condition Methods Fresh human lung tissues were obtained from the patients undergoing a chest operation hPASMCs were isolated, cultured, and divided into 6 groups: ① control group, cultured under normoxia; ② diazoxide group, cultured in normoxia with diazoxide, an opener of MitoK ATP ; ③ 5 HD group, cultured in normoxia with sodium 5 hydroxydecanoate (5 HD), an antagonist of MitoK ATP ; ④ 24 hour hypoxia group; ⑤ 24 hour hypoxia + diazoxide group; and ⑥ 24 hour hypoxia + 5HD group Whole cell patch clamp technique was used to trace the cell membrane K + currents The expressions of cell membrane Kv1 5 mRNA and protein were determined by RT PCR and Western blot technique, respectively The relative changes in mitochondrial potential were tested with rhodamine fluorescence (R 123) technique Results After exposure to diazoxide for 24 hours, the intensity of R 123 fluorescence in normoxic hPASMCs was significantly increased compared with control group ( P <0 05), but there were no significant changes in these tests after the hPASMCs had been exposed to 5 HD for 24 hours Twenty four hour hypoxia or 24 hour hypoxia + diazoxide could markedly increase the intensity of R 123 fluorescence in hPASMC and the changes were more significant in 24 hour hypoxia +diazox

关键词： pulmonary arterial smooth muscle cell · anoxia · membrane potentials · mitochondrial membrane · potassium channel

Effect of vascular endothelial growth factor and its receptor KDR on human airway smooth muscle cells proliferation

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Journal 2005年第7期118卷 591-594页

作者： ZOUhui xuyong-jian ZHANGZhen-xiang DepartmentofRespiratoryMedicine TongjiHospitalTongjiMedicalCollegeHuazhongUniversityofScienceandTechnologyWuhan430030China

Airway remodeling with inflammatory cell infiltration, epithelial shedding, basement membrane thickening and increased mass of airway smooth muscle (ASM) is an important determinant of bronchial obstruction and hyperresponsiveness in asthma.^(1,2) Increased ASM mass is by far the most important abnormality responsible for excessive airway narrowing and compliance of the airway wall in asthma.^(1-3) ASM growth and proliferation in asthma is a complex phenomenon of which the underlying mechanisms are difficult to investigate in vivo. The increased amount of ASM in asthmatics is an indication of abnormal cell proliferation and growth, but little is known regarding the molecular mechanisms and factors that regulate ASM cell proliferation and growth in asthma.

关键词： vascular endothelial growth factor kinase-insert-domain-containing receptor airway smooth muscle cells proliferation

Focal adhesion kinase antisense oligodeoxynucleotides inhibit human pulmonary artery smooth muscle cells proliferation and promote human pulmonary artery smooth muscle cells apoptosis

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Journal 2005年第1期118卷 20-26页

作者： LINChun-long ZHANGZhen-xiang xuyong-jian NIWang CHENShi-xin RespiratoryDepartment TongjiHospitalTongjiMedicalSchoolHuazhongUniversityofScienceandTechnologyWuhan430030China RespiratoryDepartment TongjiHospitalTongjiMedicalSchoolHuazhongUniversityofScienceandTechnologyWuhan430030China

Background Pulmonary artery smooth muscle cell (PASMC) proliferation plays an important role in pulmonary vessel structural remodelling At present, the mechanisms related to proliferation of PASMCs are not clear Focal adhesion kinase (FAK) is a widely expressed nonreceptor protein tyrosine kinase Recent research indicates that FAK is implicated in signalling pathways which regulate cytoskeletal organization, adhesion, migration, survival and proliferation of cells Furthermore, there are no reports about the role of FAK in human pulmonary artery smooth muscle cells (HPASMCs) We investigated whether FAK takes part in the intracellular signalling pathway involved in HPASMCs proliferation and apoptosis, by using antisense oligodeoxynucleotides (ODNs) to selectively suppress the expression of FAK protein Methods Cultured HPASMCs stimulated by fibronectin (40 μg/ml) were passively transfected with ODNs, sense FAK, mismatch sense and antisense FAK respectively Expression of FAK, Jun NH2 terminal kinase (JNK), cyclin dependent kinase 2 (CDK 2) and caspase 3 proteins were detected by immunoprecipitation and Western blots Cell cycle and cell apoptosis were analysed by flow cytometry In addition, cytoplasmic FAK expression was detected by immunocytochemical staining Results When compared with mismatch sense group, the protein expressions of FAK, JNK and CDK 2 in HPASMCs decreased in antisense FAK ODNs group and increased in sense FAK ODNs group significantly Caspase 3 expression upregulated in HPASMCs when treated with antisense ODNs and downregulated when treated with sense ODNs When compared with mismatch sense ODNs group, the proportion of cells at G 1 phase decreased significantly in sense ODNs group, while the proportion of cells at S phase increased significantly In contrast, compared with mismatch sense ODNs group, the proportion of cells at G 1 phase was increased significantly in antisense FAK ODNs group The level o

关键词： human pulmonary artery smooth muscle cells · focal adhesion kinase · proliferation · apoptosis Respiratory

Experimental study of acute lung injury induced by different tidal volume ventilation in rats

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Medical Journal 2005年第9期118卷 777-780页

作者： ZHANGXin-ri DUYong-cheng jianGHong-ying XUjian-ying xuyong-jian DepartmentofRespiratoryMedicine TongjiHospitalTongjiMedicalCollageHuazhongScienceandTechnologyUniversityWuhan430030China DepartmentofRespiratoryMedicine FirstAffiliatedHospitalShanxiMedicalUniversityTaiyuan030001China

Mechanical ventilation (MV) is a dual blade sward which if misused could lead to lung injury, called ventilator induced lung injury (VILI). Pathogenesis of VILI is very complex with various manifestations, which is the focus in MV field in recent years. 1 In our research, the rats were ventilated with different tidal volume, then the pathological changes of the lungs were observed under macroscopy, light and electronic microscope, and various laboratory tests in blood and bronchoalveolar lavage fluid (BALF) were also carried out in order to probe further the pathologic characteristics and the pathogenesis of VILI.

关键词： mechanical ventilation · acute lung injury · neutrophils · myloperoxidase · bronchoalveolar lavage fluid

ERK signaling mediates enhanced angiotensin II-induced rat aortic constriction following chronic intermittent hypoxia

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Journal 2013年第17期126卷 3251-3258页

作者： GUO Xue-ling DENG Yan SHANG Jin LIU Kui xuyong-jian LIU Hui-guo Department of Respiratory and Critical Care Medicine Tongji Hospital Huazhong University of Science and Technology Key Laboratory of Respiratory Disease of the Ministry of Health Wuhan Hubei 430030 China

Background Obstructive sleep apnea （OSA） has been recognized as an independent risk factor for systemic hypertension. The study investigated the functional consequences of chronic intermittent hypoxia （CIH） on aortic constriction induced by angiotensin II （Ang II） and the possible signaling involving ERK1/2 and contractile proteins such as myosin light chain kinase （MLCK）, myosin phosphatase targeting subunit （MYPT1） and myosin light chain （MLC）. Methods Male Wistar rats were randomly divided into CIH group and normoxia group and exposed to either CIH procedure or air-air cycles. Phosphorylation of ERK1/2, MYPT1 and MLC was assessed by Western blotting following constrictor studies in the presence or absence of PD98059 （10 μmol/L）. Results CIH-exposure resulted in more body weight gain and elevated blood pressure, which could be attenuated by pretreatment with PD98059. Endothelium-removed aortic rings from CIH rats exhibited higher constrictor sensitivity to Ang II （Emax：（138.56±5.78）% versus （98.45±5.31）% of KCI; pD2：7.98±0.14 versus 8.14±0.05, respectively）. CIH procedure exerted complex effects on ERK expressions （total ERK1/2 decreased whereas the ratio of phosphorylated to total ERK1/2 increased）. CIH aortas had higher MLCK mRNA and basal phosphorylation of MYPT1 and MLC. In parallel to greater increases in phosphorylation of ERK1/2, MYPT1 and MLC, Ang II-induced aortic constriction was significantly enhanced in CIH rats, which was largely reversed by PD98059. However vascular constriction of normoxia rats remained unchanged despite similar but smaller changing tendency of proteins phosphorylation. Conclusion These data suggest that CIH exposure results in aortic hyperresponsiveness to Ang II, presumably owing to more activated ERK1/2 signaling pathway.

关键词： chronic intermittent hypoxia ERK1/2 myosin phosphatase targeting subunit myosin light chain vasoconstriction

Murine calcium-activated chloride channel family member 3 induces asthmatic airway inflammation independently of allergen exposure

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Journal 2013年第17期126卷 3283-3288页

作者： MEI Li HE Li WU Si-si ZHANG Bo xuyong-jian ZHANG Zhen-xiang ZHAO jian-ping ZHANG Hui-lan Department of Respiratory Medicine Tongji Hospital of Tongji Medical College Huazhong University of Science and Technology Wuhan Hubei 430030 China Department of Respiratory Medicine Jingzhou Central Hospital Jingzhou Hubei 434020 China

Background Expression of murine calcium-activated chloride channel family member 3 （mCLCA3） has been reported to be increased in the airway epithelium of asthmatic mice challenged with ovalbumin （OVA）. However, its role in asthmatic airway inflammation under no OVA exposure has not yet been clarified. Methods mCLCA3 plasmids were transfected into the airways of normal BALB/c mice. mCLCA3 expression and airway inflammation in mouse lung tissue were evaluated. Cell differentials and cytokines in bronchoalveolar lavage fluid （BALF） were analyzed. The expression of mCLCA3 protein and mucus protein mucin-5 subtype AC （MUC5AC） were analyzed by Western blotting. The mRNA levels of mCLCA3, MUC5AC and interleukin-13 （IL-13） were determined quantitatively. Results mCLCA3 expression was not detected in the control group while strong immunoreactivity was detected in the OVA and mCLCA3 plasmid groups, and was strictly localized to the airway epithelium. The numbers of inflammatory cells in lung tissue and BALF were increased in both mCLCA3 plasmid and OVA groups. The protein and mRNA levels of mCLCA3 and MUC5AC in the lung tissue were significantly increased in the mCLCA3 plasmid and OVA groups compared to the control group. The level of IL-13, but not IL-4, IL-5, IFN-y, CCL2, CCL5 or CCL11, was significantly increased compared with control group in BALF in the mCLCA3 plasmid and OVA groups. The level of IL-13 in the BALF in the mCLCA3 plasmid group was much higher than that in the OVA group （P 〈0.05）. The level of mCLCA3 mRNA in lung tissue was positively correlated with the levels of MUC5AC mRNA in lung tissue, IL-13 mRNA in lung tissue, the number of eosinophils in BALF, and the content of IL-13 protein in BALE The level of IL-13 mRNA in lung tissue was positively correlated with the number of eosinophils in BALF and the level of MUC5AC mRNA in lung tissue. Conclusion These findings suggest that increased expression of a single-gene, mCLCA3, could simulate an asthma attack, a

关键词： murine calcium-activated chloride channel family member 3 asthma inflammation

In Situ Directional Polymerization of Poly(1,3-dioxolane)Solid Electrolyte Induced by Cellulose Paper-Based Composite Separator for Lithium Metal Batteries

在线全文

学校读者我要写书评

暂无评论

Energy & Environmental Materials 2023年第3期6卷 134-143页

作者： jian Ma Yueyue Wu Hao jiang Xin Yao Fan Zhang Xianglong Hou xuyong Feng Hongfa Xiang School of Materials Science and Engineering Anhui Provincial Key Laboratory of Advanced Functional Materials and DevicesHefei University of TechnologyHefei 230009China

In traditional in situ polymerization preparation for solid-state electrolytes,initiators are directly added to the liquid *** this article,a novel cellulose paper-based composite separator is fabricated,which employs alumina as the inorganic reinforcing material and is loaded with polymerization initiator aluminum *** upon this,a separator-induced in situ directional polymerization technique is demonstrated,and the extra addition of initiators into liquid precursors is no longer *** polymerization starts from the surface and interior of the separator and extends outward with the gradually dissolving of initiators into the *** with its traditional counterpart,the separator-induced poly(1,3-dioxolane)electrolyte shows improved interfacial contact as well as appropriately mitigated polymerization rate,which are conducive to practical *** measurement results show that the prepared poly(1,3-dioxolane)solid electrolyte possesses an oxidation potential up to 4.4 V and a high Li+transference number of *** 1000 cycles at 2 C rate(340 mA g^(−1)),the assembled Li||LiFePO_(4)solid battery possesses a 106.8 mAh g^(−1)discharge capacity retention and 83.5%capacity retention ratio,with high average Coulombic efficiency of 99.5%*** work may provide new ideas for the design and application of in situ polymerization technique for solid electrolytes and solid batteries.

关键词： cellulose paper-based composite separator in situ directional polymerization lithium metal battery poly-DOL electrolyte solid-state electrolyte

Enhanced Electrochemical Performance of Na_(0.67)Fe_(0.5)Mn_(0.5)O_(2)Cathode with SnO_(2)Modification

在线全文

学校读者我要写书评

暂无评论

Chemical Research in Chinese Universities 2021年第5期37卷 1130-1136页

作者： YE Peng LIU Yongchao MA jian WANG Yueda FENG xuyong XIANG Hongfa SUN Yi LIANG Xin YU Yan School of Materials Science and Engineering Engineering Research Center of High Performance Copper Alloy Materials and ProcessingMinistry of EducationAnhui Provincial Key Laboratory of Advanced Functional Materials and DevicesHefei University of TechnologyHefei230009P.R.China Hefei National Laboratory for Physical Sciences at the Microscale Department of Materials Science and EngineeringKey Laboratory of Materials for Energy ConversionChinese Academy of Sciences(CAS)University of Science and Technology of ChinaHefei230026P.R.China Dalian National Laboratory for Clean Energy(DNL) Chinese Academy of Sciences(CAS)Dalian116023P.R.China

P2-type layered oxide Na_(0.67)Fe_(0.5)Mn_(0.5)O_(2)is recognized as a very promising cathode material for sodium-ion batteries due to the merits of high capacity,high voltage,low cost,and easy ***,its unsatisfactory cycle and rate performances remain huge obstacles for practical ***,we report a strategy of SnO_(2)modification on P2-type Na_(0.67)Fe_(0.5)Mn_(0.5)O_(2)to improve the cycle and rate performance.

关键词： Cathode Electrochemical

Stable sodium anodes for sodium metal batteries(SMBs) enabled by in-situ formed quasi solid-state polymer electrolyte

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Energy Chemistry 2023年第2期77卷 290-299,I0008页

作者： jian Ma xuyong Feng Yueyue Wu Yueda Wang Pengcheng Liu Ke Shang Hao jiang Xianglong Hou David Mitlin Hongfa Xiang School of Materials Science and Engineering Anhui Provincial Key Laboratory of Advanced Functional Materials and DevicesHefei University of TechnologyHefei 230009AnhuiChina Materials Science and Engineering Program&Texas Materials Institute(TMI) The University of Texas at AustinAustinTX 78712-1591USA School of Chemistry and Chemical Engineering Anhui Provincial Key Laboratory of Advanced Functional Materials and DevicesHefei University of TechnologyHefei 230009AnhuiChina

A high-performance quasi-solid polymer electrolyte for sodium metal batteries(SMBs)based on in-situ polymerized poly(1,3-dioxolane)(DOL)with 20%volume ratio of fluoroethylene carbonate(FEC),termed"PDFE-20",is proposed in this *** is demonstrated PDFE-20 possesses a room-temperature ionic conductivity of 3.31×10^(-3) S cm^(-1),an ionic diffusion activation energy of 0.10 eV,and an oxidation potential of 4.4 *** based on PDFE-20 and Na_(3)V_(2)(PO_(4))_(3)(NVP)cathodes were evaluated with an active material mass loading of 6.8 mg cm^(-2).The cell displayed an initial discharge specific capacity of 104 mA h g^(-1),and97.1%capacity retention after 100 cycles at 0.5 ***-situ polymerization conformally coats the anode/-cathode interfaces,avoiding geometrical gaps and high charge transfer resistance with ex-situ polymerization of the same *** acts as a plasticizer during polymerization to suppress crystallization and significantly improves ionic *** battery cycling FEC promotes mechanical congruence of electrolyte-electrode interfaces while forming a stable NaF-rich solid electrolyte interphase(SEI)at the *** functional theory(DFT)calculations were also performed to further understand the role FEC in the poly(DOL)-FEC *** work broadens the application of in-situ prepared poly(DOL)electrolytes to sodium storage and demonstrates the crucial role of FEC in improving the electrochemical performance.

关键词： Quasi-solid batteries(QSBs) Quasi-solid electrolytes(QSEs) NaF-rich passivating layer Interfacial stability Sodium metal batteries(SMBs)