检索结果-南通市图书馆

Metal-amyloid-β peptide interactions: a preliminary investigation of molecular mechanisms for Alzheimer’s disease

在线全文

学校读者我要写书评

暂无评论

science china Chemistry 2007年第4期50卷 453-467页

作者： JIAO Yong & yang pin key laboratory of chemical biology and molecular engineering of ministry of education, institute of molecular science, shanxi university, taiyuan 030006, china key laboratory of chemical biology and molecular engineering of ministry of education Institute of Molecular Science Shanxi University Taiyuan China

Although humans have spent exactly 100 years combating Alzheimer’s disease (AD), the molecular mechanisms of AD remain unclear. Owing to the rapid growth of the oldest age groups of the popula-tion and the continuous increase of the incidence of AD, it has become one of the crucial problems to modern sciences. It would be impossible to prevent or reverse AD at the root without elucidating its molecular mechanisms. From the point of view of metal-amyloid-β peptide (Aβ) interactions, we review the molecular mechanisms of AD, mainly including Cu2+ and Zn2+ inducing the aggregation of Aβ, cata-lysing the production of active oxygen species from Aβ, as well as interacting with the ion-channel-like structures of Aβ. Moreover, the development of therapeutic drugs on the basis of metal-Aβ interactions is also briefly introduced. With the increasingly rapid progress of the molecular mechanisms of AD, we are now entering a new dawn that promises the delivery of revolutionary developments for the control of dementias.

关键词： Alzheimer’s disease (AD) amyloid-β peptide Cu2+ Zn2+ molecular mechanisms aggregation active oxygen species ion channel

Effects of Cu^(2+) on characteristic of SV currents

在线全文

学校读者我要写书评

暂无评论

science china Chemistry 2006年第6期49卷 560-564页

作者： ZHANG Liping1,2 & yang pin1 1. key laboratory of chemical biology and molecular engineering of ministry of education, institute of molecular science, shanxi university, taiyuan 030006, china 2. institute of Environment science, taiyuan university of science and Technology, taiyuan 030024, china key laboratory of chemical biology and molecular engineering of ministry of education Institute of Molecular Science Shanxi University Taiyuan 030006 China

Applying the patch-clamp technique to vacuoles from Radish we studied the effects of Cu2+ on Slow Vacuolar (SV) current’s characteristic. Our results show that Cu2+ in bath solution at higher concentration inhibits SV currents and the percentage of inhibition increases with increasing concentration and changes with different voltage. When at lower concentration, Cu2+ significantly promotes the SV currents and the promotion ratio decrease with increasing voltage. At the same time, the time constants of activation become lesser after adding Cu2+. These results show that there may be some Cu2+ binding sites on SV channels and binding to which can change SV current’s characteristic.

关键词： the patch-clamp technique, vacuolar, Cu2+, SV channel.

Effects of lanthanum on calcium-activated K^+ currents and its kinetics in MC3T3 cells

在线全文

学校读者我要写书评

暂无评论

science china Chemistry 2010年第6期53卷 1281-1286页

作者： ZHANG Liping1,2 & yang pin1 1key laboratory of chemical biology and molecular engineering of ministry of education institute of molecular science,shanxi university,taiyuan 030006,china 2College of Environment and Safety,taiyuan university of science and Technology,taiyuan 030024,china key laboratory of chemical biology and molecular engineering of ministry of education institute of molecular science Shanxi University Taiyuan China College of Environment and Safety Taiyuan University of Science and Technology Taiyuan China

Using the whole cell patch-clamp technique,we studied the effects of La3+ on calcium-activated K+ currents and its kinetics of activation and inactivation in non-excitable MC3T3 *** results showed that the calcium-activated outward K+ currents were promoted with increasing concentration of Ca2+ in the pipette solution and a voltage- and Ca2+-dependent ***3+ in the bath solution inhibited the currents in a concentration-dependent manner and the inhibition EC50 was 8.23 ± 1.45 μmol/*** the concentration of 50 μmol/L,La3+ significantly changed the Vh of the activation curve and shifted the activation curve to more positive potentials,but shifted the inactivation curve to more negative *** had no effect on the slope factor k of the activation and inactivation *** currents inhibition could induce a series of physiological and molecular biological functions,presumably because of its ability to depolarize the plasma membrane and enhance cell excitability,resulting in increasing Ca2+ influx and cytoplast Ca2+ *** process may be one of the molecular mechanisms by which La3+ affects the cell growth and function of MC3T3 cells.

关键词： MC3T3 cell potassium ion channel La3+ patch-clamp technique

Detection of nano Eu2O3 in cells and study of its biological effects

在线全文

学校读者我要写书评

暂无评论

Detection of nano Eu2O3 in cells and study of its biological...

Nitric oxide suppresses stomatal opening by inhibiting inward-rectifying Kin+ channels in Arabidopsis guard cells

2010中西部地区无机化学化工学术研讨会

作者： LI Ying-qi,yang pin~* (institute of molecular science,shanxi university,key laboratory of chemical biology and molecular engineering of ministry of education,taiyuan 030006,china)

The biological effect of rare earth represents the dual natures of promoting cell proliferation and *** research on the biological effect of rare earth compound has aroused wide concerns, but it remained unknown for the transmembrane and distribution under the action of rare earth oxide nanoparticle with cell as well as its biological *** the present data,it was firstly observed that the nano EuO entered the living Hela cell by endocytosis using Laser Scanning Confocal *** distribution of nano EuO was in the cytoplasm around the ***,we studied the effect of nano EuO on living cells under the condition of in vitro *** result showed that within the low concentration range(<1.0mg/ml),the nano EuO had no obvious effects on the apoptosis and the cell cycle,although the morphology appeared *** the concentration gradually rose,it had dramatic biological effects.1.0 mg/ml nano EuO caused the cellular damages and led to the vacuolation on the cell ***,it obviously promoted the apoptosis of Hela cells,which suggested that 1.0 mg/ml nano EuO induced a necrotic cell reaction with respect to the nature of cytotoxin.

关键词： Nano Eu2O3 HeLa cells Biololgical activity

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

science Bulletin 2008年第14期 2157-2160页

作者： xue shaowu1,2, yang pin2 & HE YiKun1 1 College of Life science, Capital Normal university, Beijing 100037, china 2 institute of molecular science, chemical biology and molecular engineering laboratory of ministry of education, shanxi university, taiyuan, 030006 china

We explore nitric oxide (NO) effect on K+in channels in Arabidopsis guard cells. We observed NO inhib- ited K+in currents when Ca2+ chelator EGTA (Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid) was not added in the pipette solution; K+in currents were not sensitive to NO when cytosolic Ca2+ was chelated by EGTA. NO inhibited the Arabidopsis stomatal opening, but when EGTA was added in the bath solution, inhibition effect of NO on stomatal opening vanished. Thus, it implies that NO ele- vates cytosolic Ca2+ by activating plasma membrane Ca2+ channels firstly, then inactivates K+in chan- nels, resulting in stomatal opening suppressed subsequently.

关键词： inward-rectifying Ki+n channels nitric oxide cytosolic Ca2+ stomata

Structures and Properties of Cytosine-BX3（X=F,Cl） Complexes: An Investigation with DFT and MP2 Methods

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Structures and Properties of Cytosine-BX3（X=F,Cl） Complexe...

Light-Mediated Spatiotemporally Dynamic Assembly of DNA Nanostructures in Living Cells Regulates Autophagy

中国化学会第九届全国量子化学学术会议

作者： Shiguo Zhang,pin yang key laboratory of chemical biology and molecular engineering of ministry of education, institute of molecular science, shanxi university, taiyuan 030006 Department of Computer science and Technology, Binzhou university, Binzhou 256600

＜正＞For the cytosine-BX3（X=F,Cl） systems, geometries and binding energies were calculated using the B3LYP method of DFT and MP2 method of ab initio with 6-311+G* or 6-311++G* bases sets. Three isomers were found for each system, and then single-point energy evaluations were performed using the larger basis sets (6-311+G（2df,p） with DFT method. In the most stable isomers of cytosine-BF3 or cytosine-BCl3, boron atom of BX3（X=F,Cl） connected to carbonyl oxygen O8 of cytosine with a stabilization energy of -78.71 and -69.62 kJ/mol at B3LYP/6-311+G* level (BSSE

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

CCS Chemistry 2024年第6期6卷 1557-1570页

作者： Rui Zhang pin Hu Yuwei Xu Ziqing Wang Dayong yang Chi Yao Frontiers science Center for Synthetic biology Key Laboratory of Systems Bioengineering(MOE)School of Chemical Engineering and TechnologyTianjin UniversityTianjin 300350 Department of Chemistry State Key Laboratory of Molecular Engineering of PolymersShanghai Key Laboratory of Molecular Catalysis and Innovative MaterialsCollege of Chemistry and MaterialsFudan UniversityShanghai 200438

The assembly of exogenous artificial architectures inside cells can regulate a series of biological events,which heavily relies on the development of spatiotemporally controlled molecular assembly *** herein report a designer deoxyribonucleic acid(DNA)nanostructure that enables light-mediated spatiotemporally dynamic assembly in living cells and consequently achieves efficient regulation of cell *** DNA nanostructure was constructed from i-motif moiety-containing branched DNA,photocleavable bond-containing linker,and tumor cell-targeting *** cellular uptake mediated by aptamers,under the spatiotemporal control of both UV light and late endosomal/lysosomal acidic environments,disassembly/reassembly of DNA nanostructure occurred via two rationally designed routes,generating microsized DNA *** a result,autophagy was significantly enhanced with the increase of DNA assembly *** enhanced autophagy showed an impact on related biological *** system is expected to be a powerful tool for the regulation of intracellular events and cellular behaviors.

关键词： DNA nanotechnology DNA hydrogel self-assembly autophagy i-motif

Investigation on Vibrational Spectra and Structures of 4-Mercaptopyridine Monomer and Its Dihydrate

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

chemical Research in Chinese Universities 2006年第1期22卷 90-93页

作者： CHENG Jian-bo 1,2 , xue Wei-qing 1 , ZHAO Bing 1** , ZHANG Gang 3 and GONG Bao-an 2 1. key laboratory for Supramolecular Structure and Material of ministry of education, Jilin university, Changchun 130012, P. R. china 2. science and engineering College of Chemistry and biology, Yantai university, Yantai 264005, P. R. china 3. State key laboratory of Theoretical and Computational Chemistry, Jilin university, Changchun 130023, P. R. china key laboratory for Supramolecular Structure and Material of ministry of education Jiln University Changchun 130012 P. R. China science and engineering College of Chemistry and biology Yantai University Yantai 264005 P. R. China State key laboratory of Theoretical and Computational Chemistry Jiln University Changchun 130023 P. R. China

The optimized molecular structures and vibrational frequencies of 4-mercaptopyridine(4MPY) monomer and its dihydrate were studied by means of the density functional theory(DFT), viz. B3LYP method with the 6-311++G(d,p) basis set. On the basis of the calculations, the assignments of the vibrational spectra of the monomer and the dihydrate were performed, and so were investigated the changes in the structure and the vibrational spectrum of the dihydrate as well as the intermolecular force resulting in the formation of the dihydrate. The calculated results show that each of the water molecule planes is vertical to the pyridine ring plane in the dihydrate that is formed via the H-bonds between 4MPY and water molecules. Furthermore, the structure and the vibrational spectrum of 4MPY can be consi- derably affected by the water molecules.

关键词： 4MPY Dihydrate Vibrational spectra DFT molecular structure

Mitochondrial sensitive probe with aggregation-induced emission characteristics for early brain diagnosis of Parkinson’s disease

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Aggregate

Aggregate 2024年第1期5卷 216-227页

作者： Liwen Huang Yutong Zhou Di Jiao Jing Ren Yilin Qi Heping Wang yang Shi Dan Ding xue xue State key laboratory of Medicinal chemical biology College of PharmacyNankai UniversityTianjinP.R.China State key laboratory of Medicinal chemical biology Key Laboratory of Bioactive MaterialsMinistry of Educationand College of Life SciencesNankai UniversityTianjinP.R.China

The early diagnosis of Parkinson’s disease(PD)provides opportunities for early intervention to slow the progression of neurological degeneration in patients,particularly as the aging population increases in our *** a series of pathological features of PD,mitochondria abnormalities have been identified as central event that occurs at the early stage of ***,the method for detecting mitochondrial abnormalities-associated early PD has not been fully *** herein report a specifically mitochondrial targeting probe(named TPA-BT-SCP)that is able to characterize mitochondria abnormalities for early diagnosis of PD and monitor PD neurodegenerative *** probe is an aggregation-induced emission(AIE)probe with a strong positive charge,a 3D distorted molecular structure,and a separated HOMO-LUMO distribution,designed with unique molecular design *** research demonstrated that TPA-BT-SCP could emit stable and strong fluorescence,and rapidly accumulate in mitochondria due to the negative *** intranasal administration of 1-methy-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)-induced PD mice,TPA-BT-SCP successfully bypassed the blood−brain barrier to light up the brain,allowing the grading of PD severity based on its high *** together,this work develops a novel AIE probe that exhibits dramatically high sensitivity to mitochondrial changes and enables noninvasive diagnosis of early PD in the brain.

关键词： aggregation-induced emission early diagnosis Parkinson’s disease