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Journal of Integrative Agriculture 2012年第2期11卷 263-268页

作者： WU Jian-xiang SHANG Hai-li XIE Yan SHEN qing-tang ZHOU xue-ping Institute of Biotechnology Zhejiang UniversityHangzhou 310029P.R.China

Tomato yellow leaf curl virus（TYLCV）is a species of the family Geminiviridae,causing serious yield losses in tomato *** coat protein（CP）gene of TYLCV isolate SH2 was expressed in Escherichia coli BL21（DE3）using pET-32a as the expression *** recombinant protein was purified through Ni＋-NTA affinity column and used to immunize BALB/c *** hybridoma cell lines（2B2,2E3 and 3E10）secreting monoclonal antibodies（MAbs）against TYLCV CP were obtained by fusing mouse myeloma cells（Sp 2/0）with spleen cells from the immunized BALB/c *** titers of ascitic fluids of three MAbs ranged from 10-6 to 10-7 in *** and subclasses of all the MAbs belonged to IgG1,κ light *** antibody sandwich enzyme-linked immunosorbent assay（TAS-ELISA）showed that the MAb 3E10 could react with five begomoviruses infecting tomato,while the other two（2B2 and 2E3）mainly reacted with ***-ELISA was set up using the MAb 3E10,and the established method could successfully detect virus in plant sap at 1：2 560（w/v,g mL-1）.Detection of field samples showed that begomoviruses were common in tomato crops in Zhejiang Province,China.

关键词： Tomato yellow leaf curl virus coat protein monoclonal antibody TAS-ELISA

BF_3·Et_2O promoted conjugate addition of ethanethiol to electron-deficient alkynes

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Chinese Chemical Letters 2012年第6期23卷 639-642页

作者： qing Fa Zhou xue Ping Chu Shen Zhao Tao Lu Wei Fang tang Department of Organic Chemistry China Pharmaceutical University Nanjing 210009 China

An effective method for the synthesis of vinyl thioethers through the conjugate addition of ethanethiol to electron-deficient alkynes promoted by ***20 has been developed. Electron-deficient internal alkynes react with ethanethiol in this system to yield mainly Z-isomer of vinyl thioether adducts, while electron-deficient terminal alkynes afford mainly E-isomer of vinyl thioether adducts.

关键词： Vinyl thioether Electron-deficient alkyne Conjugate addition Synthesis

Recent progress and prospect of carbon-free single-site catalysts for the hydrogen and oxygen evolution reactions

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Nano Research 2022年第2期15卷 818-837页

作者： Jingqi Guan xue Bai Tianmi tang Institute of Physical Chemistry College of ChemistryJilin University2519 Jiefang RoadChangchun 130021China

The key challenge for scalable production of hydrogen from water lies in the rational design and preparation of high-performance and earth-abundant electrocatalysts to replace precious metal Pt and IrO_(2) for hydrogen evolution reaction(HER)and oxygen evolution reaction(OER).Although atomic M-N-C materials have been extensively studied in heterogeneous catalysis field,the insufficient antioxidant capacity of carbonous substrates hinders their practical application in *** highly active and stable OER electrocatalysts is the key for electrochemical water *** review presents feasible design strategies for fabricating carbon-free single-site catalysts and their applications in HER/OER and overall water *** constitutive relationships between structure,composition,and catalytic performance for HER and OER are detailly discussed,providing ponderable insights into rationally constructing high-performance HER and OER *** perspectives on the challenges and future research orientations in single-site catalysts for electrochemical water splitting are suggested.

关键词： single-atom catalyst single-site catalyst hydrogen evolution reaction oxygen evolution reaction water splitting

The Austrian node of the natural resources satellite remote sensing cloud service platform:examples of Sino-Austrian cooperation

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Geo-Spatial Information Science 2021年第1期24卷 145-151,I0015页

作者： Wolfgang Kainz Xinming tang Yucai xue Department of Geography and Regional Research University of ViennaViennaAustria Land Satellite Remote Sensing Application Center Ministry of Natural Resources of the Peoples Republic of ChinaBeijingChina

The Austrian node of the Natural Resources Satellite Remote Sensing Cloud Service Platform was established in 2016 through a cooperation agreement between the Land Satellite Remote Sensing Application Center(LASAC),Ministry of Natural Resources of the Peoples Republic of China and the University of Vienna,*** this agreement panchromatic and multi-spectral data of the Chinese ZY-3 satellite are pushed to the server at the University of Vienna for use in education and *** far,nearly 500 GB of data have been uploaded to the *** technical note briefly introduces the ZY-3 system and illustrates the implementation of the agreement by the first China-Sat Workshop and several case *** of them are already completed,others are still *** include a geometric accuracy validation of ZY-3 data,an animated visualization of image quick views on a spherical display to demonstrate the time series of the image coverage for Austria and Laos,and the use of ZY-3 data to study the spread of bark beetle in the province of Lower *** accuracy study of DTMs from ZY-3 stereo data,as well as a land cover analysis and comparison of Austria with ZY-3 and other sensors are still ongoing.

关键词： Remote sensing geographic information systems ZY-3 satellite hyperglobe Land Satellite Remote Sensing Application Center(LASAC)

Microstructure of ferrospheres in fly ashes: SEM, EDX and ESEM analysis

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2008年第11期9卷 1595-1600页

作者： qing-feng xue Sheng-gao LU Zhejiang Provincial Key Laboratory of Subtropical Soil and Plant Nutrition College of Environmental and Resource Sciences Zhejiang University Hangzhou 310027 China

Ferrospheres in fly ashes from a coal-fired power plant were extracted by a magnetic separation technique and their microstructure was studied by scanning electron microscopy (SEM), energy dispersive X-ray analysis (EDX) and environmental scanning electron microscopy (ESEM). Ferrospheres in fly ashes show significant iron enrichment compared to their respective fly ashes. Iron oxides in ferrospheres mainly occur as minerals magnetite (Fe3O4) and hematite (α-Fe2O3), which are derived mainly from the decomposition and oxidation of iron-bearing minerals in coal during combustion. EDX data indicate that ferrospheres also contain Si, S, Al and Ca resulting from quartz, mullite, anhydrite and amorphous materials. A large percentage of ferrospheres are commonly 5～50 μm in size. The microstructure of ferrospheres includes smooth, polygonal, dendritic, granular and molten drop characteristics. SEM coupled with EDX provided fast and accurate results of the microstructure and chemical composition of ferrospheres, and helped us to assess environmental issues related to the disposal and utilization of fly ashes.

关键词： Fly ash Ferrosphere Microstructure Scanning electron microscopy (SEM)

A predictive model of response to metoprolol in children and adolescents with postural tachycardia syndrome

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World Journal of Pediatrics 2023年第4期19卷 390-400页

作者： Bo-Wen Xu qing-You Zhang xue-Ying Li Chao-Shu tang Jun-Bao Du xue-Qin Liu Hong-Fang Jin Department of Pediatrics Peking University First HospitalNo.1Xi’an-Men StreetWest DistrictBeijing100034China Department of Medical Statistics Peking University First HospitalBeijingChina Key Lab of Molecular Cardiovascular Sciences Ministry of EducationBeijingChina Department of Physiology and Pathophysiology Peking University Health Science CentreBeijingChina

Background The present work was designed to explore whether electrocardiogram(ECG)index-based models could predict the effectiveness of metoprolol therapy in pediatric patients with postural tachycardia syndrome(POTS).Methods This study consisted of a training set and an external validation *** and adolescents with POTS who were given metoprolol treatment were enrolled,and after follow-up,they were grouped into non-responders and responders depending on the efficacy of *** difference in pre-treatment baseline ECG indicators was analyzed between the two groups in the training *** logistic regression analysis was further conducted on the association between significantly different baseline variables and therapeutic *** models were established to predict therapeutic response to *** receiver-operating characteristic curve(ROC),calibration,and internal validation were used to evaluate the prediction *** predictive ability of the model was validated in the external validation *** Of the 95 enrolled patients,65 responded to metoprolol treatment,and 30 failed to *** the responders,the maximum value of the P wave after correction(Pcmax),P wave dispersion(Pd),Pd after correction(Pcd),QT interval dis-persion(QTd),QTd after correction(QTcd),maximum T-peak-to-T-end interval(Tpemax),and T-peak-to-T-end interval dispersion(Tped)were prolonged(all P<0.01),and the P wave amplitude was increased(P<0.05)compared with those of the *** contrast,the minimum value of the P wave duration after correction(Pcmin),the minimum value of the QT interval after correction(QTcmin),and the minimum T-peak-to-T-end interval(Tpemin)in the responders were shorter(P<0.01,<0.01 and<0.01,respectively)than those in the *** above indicators were screened based on the clinical significance and multicollinearity analysis to construct a binary logistic *** a result,pre-treatment Pcmax,QTcmin,and Tped

关键词： Children Electrocardiography Metoprolol Nomogram Predictor Postural tachycardia syndrome

Relationship between copper content of slag and matte in the SKS copper smelting process

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International Journal of Minerals,Metallurgy and Materials 2019年第3期26卷 301-308页

作者： Qin-meng Wang Song-song Wang Miao Tian Ding-xuan tang qing-hua Tian xue-yi Guo School of Metallurgy and Environment Central South University Hunan Key Laboratory of Nonferrous Metal Resources Recycling School of Mineral Processing and Bioengineering Central South University

In the newly developed oxygen-enriched bottom-blowing copper smelting process(also known as the SKS copper smelting process), Cu loss in slag is one of the most concerning issues. This paper presents our research results concerning the relationship between the Cu content of the matte and slag in the SKS process; the results are based on actual industrial production in the Dongying Fangyuan copper smelter. The results show that the matte grade strongly influences Cu losses in slag. The dissolved and entrained losses account for 10%–20% and 80%–90% of the total SKS industrial Cu losses in slag, respectively. With increasing matte grade, the dissolved and entrained Cu losses in the SKS slag both increase continuously. When the matte grade is greater than 68%, the content of Cu in the smelting slag increases much more dramatically. To obtain a high direct recovery of copper, the matte grade should be less than 75% in industrial SKS copper production.

关键词： copper smelting oxygen-enriched bottom-blowing matte grade Cu loss SKS process

A Computer Graphics-Based Framework for 3D Pose Estimation of Pedestrians

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Automotive Innovation 2021年第3期4卷 338-349页

作者： Jisi tang qing Zhou State Key Laboratory of Automotive Safety and Energy School of Vehicle and MobilityTsinghua UniversityBeijing 100084China

In pedestrian-to-vehicle collision accidents,adapting safety measures ahead of time based on actual pose of pedestrians is one of the core objectives for integrated *** can significantly enhance the performance of passive safety system when active safety maneuvers fail to avoid *** study proposes a deep learning model to estimate 3D pose of pedestrians from *** conventional pedestrian image datasets do not have available pose features to work with,a computer graphics-based(CG)framework is established to train the system with synthetic *** 3D meshes of standing males are first transformed into several walking poses,and then rendered as images from multiple view ***,a matrix of 50 anthropometries,10 gaits and 12 views is built,in total of 6000 images.A two-branch convolutional neural network(CNN)was trained on the synthetic *** model can simultaneously predict 16 joint landmarks and 14 joint angles of pedestrian for each image with high *** errors of the predictions are 0.54 pixels and−0.06°,*** specific pose can then be completely reconstructed from the ***,the current study has established a CG-based pipeline to generate photorealistic images with desired features for the training;it demonstrates the feasibility of leveraging CNN to estimate the pose of a walking pedestrian from synthesized *** proposed framework provides a starting point for vehicles to infer pedestrian poses and then adapt protection measures accordingly for imminent impact to minimize pedestrian injuries.

关键词： Pose estimation Pedestrian safety Computer graphics Convolutional neural network

Influence of Cl substitution on the electronic structure and catalytic activity of ceria

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Science China Chemistry 2015年第4期58卷 601-606页

作者： Li-Li Yin xue-qing Gong Key Laboratory for Advanced Materials Centre for Computational Chemistry and Research Institute of Industrial Catalysis East China University of Science and Technology

Cl-containing cerium dioxide(Ce O2) catalysts have been found to exhibit unique catalytic activities. In the present work, using density functional theory calculations with the inclusion of on-site Coulomb correction, we systematically studied the effect of Cl on the physicochemical properties of Ce O2 surfaces by substituting one subsurface O with Cl. The calculated results show that substituting an O atom with a Cl atom results in structural distortion and the reduction of one surface Ce4+ cation to Ce3+. The protruding Ce3+ cation greatly improves the adsorption energy of O2 to produce an active O2- species, and maintains the catalytic oxidation cycle of CO on Ce O2(110). These results may help us obtain a better understanding of Cl-ceria interacting systems and provide some guidance for the design of effective Ce O2-based catalysts.

关键词： cerium dioxide Cl substitution DFT+U O2 adsorption CO oxidation

不同内固定系统治疗股骨转子间骨折的力学稳定性

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中国组织工程研究 2025年第9期29卷 1783-1788页

作者：陈曦汤涛陈铜兵李青张文常州市第一人民医院江苏省常州市213003 苏州大学附属第三医院江苏省常州市213003 苏州大学苏州医学院骨科研究所江苏省苏州市215006

背景:股骨转子间骨折的骨折类型和固定方式多样,各固定系统间的力学稳定性相差较大。使用有限元分析方法对各固定系统开展生物力学研究具有科学的临床意义。目的:通过有限元方法对比分析多种内固定应用于股骨A031-A2.1型转子间骨折的力... 详细信息

背景:股骨转子间骨折的骨折类型和固定方式多样,各固定系统间的力学稳定性相差较大。使用有限元分析方法对各固定系统开展生物力学研究具有科学的临床意义。目的:通过有限元方法对比分析多种内固定应用于股骨A031-A2.1型转子间骨折的力学稳定性。方法:在已验证有效性的股骨有限元模型基础上,对模型进行必要的切割,造模成股骨A031-A2.1型转子间骨折,模拟临床手术方法置入不同的内固定系统,分别建立股骨近端防旋髓内钉、动力髋螺钉、经皮加压钢板和股骨近端锁定钢板固定模型。约束4组模型股骨远端下所有节点,在股骨头上施加700,1400和2100N的压缩载荷,通过计算分析,观察各组模型的等效应力分布和压缩刚度,比较各组模型之间的力学稳定性。结果与结论:①通过计算分析,对比各组模型的变形量计算压缩刚度后,在各级载荷作用下,各组模型上压缩刚度呈现的趋势:生理组>股骨近端防旋髓内钉组>股骨近端锁定钢板组>经皮加压钢板组>动力髋螺钉组;完整的生理组模型的压缩刚度明显高于所有的手术组模型;②观察应力指标,因存在应力遮挡效应,致使各固定组的应力峰值均高于生理组,最大峰值均集中分布于各内固定上;其中股骨近端防旋髓内钉固定组应力峰值最小,动力髋螺钉固定组应力最高,应力分布趋势呈现:生理组<股骨近端防旋髓内钉固定组<经皮加压钢板固定组<股骨近端锁定钢板固定组<动力髋螺钉固定组;③分布于骨质模型的应力,因内固定置入位置不同呈现不同的分布结果;④提示对于股骨转子间骨折,各种内固定均能起到有效的固定,结合有限元分析结果得出股骨近端防旋髓内钉组是较好的一种内固定选择,呈现变形量小、应力峰值低,应力分布均匀的特性;经皮加压钢板组和股骨近端锁定钢板组的力学效果优良,固定效果接近股骨近端防旋髓内钉固定;动力髋螺钉固定效果欠佳,同比于其他内固定,其力学稳定性较差。

关键词：股骨转子间骨折内固定系统力学稳定性有限元分析等效应力压缩刚度