Monoclonal Antibodies Against the Whitefly-Transmitted Tomato Yellow Leaf Curl Virus and Their Application in Virus Detection

作     者：WU Jian-xiang SHANG Hai-li XIE Yan SHEN Qing-tang ZHOU Xue-ping 

作者机构：Institute of BiotechnologyZhejiang UniversityHangzhou 310029P.R.China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2012年第11卷第2期

页      面：263-268页

核心收录：

学科分类：09[农学] 

基　　金：supported by the Special Fund for Agro-Scientific Research in the Public Interest from the Ministry of Agriculture China(201003065) 

主　　题：Tomato yellow leaf curl virus coat protein monoclonal antibody TAS-ELISA 

摘      要：Tomato yellow leaf curl virus（TYLCV）is a species of the family Geminiviridae,causing serious yield losses in tomato *** coat protein（CP）gene of TYLCV isolate SH2 was expressed in Escherichia coli BL21（DE3）using pET-32a as the expression *** recombinant protein was purified through Ni＋-NTA affinity column and used to immunize BALB/c *** hybridoma cell lines（2B2,2E3 and 3E10）secreting monoclonal antibodies（MAbs）against TYLCV CP were obtained by fusing mouse myeloma cells（Sp 2/0）with spleen cells from the immunized BALB/c *** titers of ascitic fluids of three MAbs ranged from 10-6 to 10-7 in *** and subclasses of all the MAbs belonged to IgG1,κ light *** antibody sandwich enzyme-linked immunosorbent assay（TAS-ELISA）showed that the MAb 3E10 could react with five begomoviruses infecting tomato,while the other two（2B2 and 2E3）mainly reacted with ***-ELISA was set up using the MAb 3E10,and the established method could successfully detect virus in plant sap at 1：2 560（w/v,g mL-1）.Detection of field samples showed that begomoviruses were common in tomato crops in Zhejiang Province,China.