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Simultaneous detection of seven phenolic acids in Danshen injection using HPLC with ultraviolet detector

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2008年第9期9卷 728-733页

作者： jin-zhong xu, jie shen, yi-yu cheng, hai-bin qu (Department of Chinese Medicine Science and Engineering, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China) Department of Chinese Medicine Science and Engineering College of Pharmaceutical Sciences Zhejiang University Hangzhou 310058 China.

A high-performance liquid chromatographic (HPLC) method with ultraviolet (UV) detector had been developed for simultaneous quantification of danshensu, protocatechuic aldehyde, caffeic acid, salvianolic acid D, rosmarinic acid, salvianolic acid B and salvianolic acid A in Danshen injection. According to the UV spectra of these components, three detection wavelengths have been selected as follows: 280 nm for danshensu and protocatechuic aldehyde, 326 nm for caffeic acid, salvianolic acid D and rosmarinic acid, 286 nm for salvianolic acid B and salvianolic acid A. The limit of detection (LOD) was improved to be in the range of 0.008~0.160 μg/ml. Moreover, excellent linear behavior over the investigated concentration range was observed, with R>0.999 for all the analytes.

关键词： High-performance liquid chromatography (HPLC) Danshen injection (DSI) Phenolic acids Multi-wavelengths quantification

Simultaneous detection of seven phenolic acids in Danshen injection using HPLC with ultraviolet detector

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浙江中西医结合杂志 2008年第9期18卷 728-733页

作者： jin-zhong xu jie shen yi-yu cheng hai-bin qu Department of Chinese Medicine Science and Engineering College of Pharmaceutical Sciences Zhejiang University Hangzhou 310058 China

A high-performance liquid chromatographic (HPLC) method with ultraviolet (UV) detector had been developed for simultaneous quantification of danshensu, protocatechuic aldehyde, caffeic acid, salvianolic acid D, rosmarinic acid, salvianolic acid B and salvianolic acid A in Danshen injection。According to the UV spectra of these components, three detection wavelengths have been selected as follows: 280 nm for danshensu and protocatechuic aldehyde, 326 nm for caffeic acid, salvianolic acid D and rosmarinic acid, 286 nm for salvianolic acid B and salvianolic acid A。The limit of detection (LOD) was improved to be in the range of 0.008-0,160μg/ml。Moreover, excellent linear behavior over the investigated concentration range was observed, with R>0.999 for all the analytes。

关键词：石炭酸丹参同步检测技术化学分析

磷钼钨酸-干酪素法测定丹参药材中鞣质的含量

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分析化学 2008年第4期36卷 553-555页

作者：沈洁徐金钟胡济宏程翼宇瞿海斌浙江大学药学院正大青春宝药业有限公司

建立了磷钼钨酸/干酪素法测定丹参药材中鞣质含量的方法。采用室温浸提过夜提取鞣质,在磷钼钨酸与多酚类物质的显色反应进行到90120min时,于760nm波长处,分别测定供试品溶液中的总酚和不被吸附的多酚的吸光度,以吸光度之差用标准曲线法... 详细信息

建立了磷钼钨酸/干酪素法测定丹参药材中鞣质含量的方法。采用室温浸提过夜提取鞣质,在磷钼钨酸与多酚类物质的显色反应进行到90120min时,于760nm波长处,分别测定供试品溶液中的总酚和不被吸附的多酚的吸光度,以吸光度之差用标准曲线法求得鞣质含量,线性范围为2.008.00mg/L,线性关系良好(r=0.9997)。方法具有较高的灵敏度与选择性,精密度和重复性均较好,其RSD分别为0.4%和6.9%,标准加入回收率在80.2%106%之间,RSD为11.8%。该方法简单、准确,并应用于不同来源的丹参药材的检测,获得了满意的结果。可用于监测丹参药材以及丹参注射液生产过程中鞣质含量的变化。

关键词：分光光度法磷钼钨酸干酪素鞣质丹参

The heterogeneity of islet autoantibodies and the progression of islet failure in type 1 diabetic patients

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Science China(Life Sciences) 2016年第9期59卷 930-939,938-939+932-937页

作者： jin Liu Lingling Bian Li Ji Yang Chen Heng Chen Yong Gu bingqin Ma Wei Gu Xinyu xu yun Shi Jian Wang Dalong Zhu Zilin Sun Jianhua Ma Hui jin Xing Shi Heng Miao bing Xin Yan Zhu Zhenwen Zhang Ruifang Bu Lan xu Guangde Shi Wei Tang Wei Li Dongmei Zhou Jun Liang Xingbo cheng Bimin Shi Jixiang Dong Ji Hu Chen Fang Shao zhong Weinan yu Weiping Lu chenguang Wu Li Qian Jiancheng yu Jialin Gao Xiaoqiang Fei Qingqing Zhang xueqin Wang Shiwei Cui jinluo cheng Ning xu Guofeng Wang Guoqing Han Chunrong xu yun Xie Minmin An Wei Zhang Zhixiao Wang yun Cai Qi Fu yu Fu Shuai Zheng Fan Yang Qingfang Hu Hao Dai yu jin Zheng Zhang Kuanfeng xu yifan Li jie shen Hongwen Zhou Wei He xuqin Zheng Xiao Han Liping yu jinxiong She Mei Zhang Tao Yang Department of Endocrinology and Metabolism First Affiliated Hospital of Nanjing Medical University Nanjing 210029 China Barbara Davis Center for Childhood Diabetes University of Colorado Denver Aurora Colorado 80045 USA Key Laboratory of Human Functional Genomics of Jiangsu Province Nanjing Medical University Nanjing 210000 China Department of Isotopic Laboratory of Nanjing Medical University Nanjing 210000 China HLA Laboratory of Jiangsu Province People's Hospital Nanjing 210029 China Department of Endocrinology and Metabolism Nanjing General Hospital of Nanjing Military Command Nanjing 210002 China Department of Endocrinology and Metabolism Nanjing Drum Tower Hospital The Affiliated Hospital of Nanjing University Medical School Nanjing 210008 China Department of Endocrinology and Metabolism Zhongda Hospital Southeast University Nanjing 210009 China Department of Endocrinology and Metabolism Nanjing First Hospital Nanjing 210000 China Department of Endocrinology and Metabolism Nanjing Children's Hospital Nanfing 210008 China Department of Endocrinology and Metabolism The Second Affiliated Hospital of Nanjing Medical University Nanjing 210011 China Department of Endocrinology and Metabolism Nanjing Governmental Hospital Nanjing 210008 China Department of Endocrinology and Metabolism Northern Jiangsu People's Hospital Yangzhou 225001 China Department of Endocrinology and Metabolism Wuxi People's Hospital Wuxi 214023 China Department of Endocrinology and Metabolism Jiangsu Jiangyin People's Hospital Wuxi 214400 China Department of Endocrinology and Metabolism The Affiliated Hospital of Xuzhou Medical College Xuzhou 221006 China Department of Endocrinology and Metabolism Xuzhou Central Hospital Xuzhou 221009 China Department of Endocrinology and Metabolism The First Affiliated Hospital of Soochow University Suzhou 215006 China Department of Endocrinology and Metabolism The Second Affiliated Hospital of Soochow University Suzhou 215004 China Department of Endocri

Type 1 diabetes mellitus is heterogeneous in many facets. The patients suffered from type 1 diabetes present several levels of islet function as well as variable number and type of islet-specific autoantibodies. This study was to investigate prevalence and heterogeneity of the islet autoantibodies and clinical phenotypes of type 1 diabetes mellitus; and also discussed the process of islet failure and its risk factors in Chinese type 1 diabetic patients. A total of 1,291 type 1 diabetic patients were enrolled in this study. Demographic information was collected. Laboratory tests including mixed-meal tolerance test, human leukocyte antigen alleles, hemoglobin A1 c, lipids, thyroid function and islet autoantibodies were conducted. The frequency of islet-specific autoantibody in newly diagnosed T1 DM patients(duration shorter than half year) was 73% in East China. According to binary logistic regressions, autoantibody positivity, longer duration and lower Body Mass Index were the risk factors of islet failure. As the disease developed, autoantibodies against glutamic acid decarboxylase declined as well as the other two autoantibodies against zinc transporter 8 and islet antigen 2. The decrease of autoantibodies was positively correlated with aggressive beta cell destruction. Autoantibodies can facilitate the identification of classic T1 DM from other subtypes and predict the progression of islet failure. As there were obvious heterogeneity in autoantibodies and clinical manifestation in different phenotypes of the disease, we should take more factors into consideration when identifying type 1 diabetes mellitus.

关键词： autoantibodies heterogeneity islet failure type 1 diabetes

Application of next-generation sequencing technology to precision medicine in cancer: joint consensus of the Tumor Biomarker Committee of the Chinese Society of Clinical Oncology

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Cancer Biology & Medicine 2019年第1期16卷 189-204页

作者： xuchao Zhang Zhiyong Liang shengyue Wang Shun Lu Yong Song ying cheng Jianming ying Weiping Liu yingyong Hou Yangqiu Li yi Liu Jun Hou Xiufeng Liu Jianyong Shao Yanhong Tai Zheng Wang Li Fu Hui Li Xiaojun Zhou Hua Bai Mengzhao Wang You Lu jinji Yang Wenzhao zhong Qing Zhou xuening Yang jie Wang cheng Huang Xiaoqing Liu Xiaoyan Zhou Shirong Zhang Hongxia Tian yu Chen Ruibao Ren Ning Liao Chunyan Wu zhongzheng Zhu Hongming Pan Yanhong Gu Liwei Wang yunpeng Liu Suzhan Zhang Tianshu Liu Gong Chen Zhimin Shao binghe xu Qingyuan Zhang Ruihua xu Lin shen yilong Wu Guangdong Lung Cancer Institute Medical Research Center Cancer Center of Guangdong Provincial People's Hospital and Guangdong Academy of Medical Sciences Affiliated Guangdong Provincial People's Hospital South China University of Technology Department of Pathology Peking Union Medical College Hospital Chinese Academy of Medical Sciences National Research Center for Translational Medicine Shanghai Rui Jin Hospital Shanghai Jiao Tong University School of Medicine Lung Tumor Clinical Medical Center Shanghai Chest Hospital Shanghai Jiao Tong University Division of Respiratory Medicine Jinling Hospital Nanjing University School of Medicine Department of Oncology Jilin Cancer Hospital Department of Pathology National Cancer Center/Cancer Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Department of Pathology West China Hospital Sichuan University Department of Pathology Zhongshan Hospital Fudan University Department of Hematology First Affiliated Hospital Institute of Hematology School of Medicine Jinan University Laboratory of Oncology Affiliated Hospital of the Academy of Military Medical Sciences Department of Oncology First Clinical College of South China University of Technology/Guangdong Lung Cancer Institute People's Liberation Army Cancer Center of Bayi Hospital Affiliated to Nanjing University of Chinese Medicine Department of Pathology Sun Yat-sen University Cancer Center Department of Pathology Affiliated Hospital of the Academy of Military Medical Sciences Department of Pathology Beijing Hospital Department of Breast Cancer Pathology and Research Laboratory of Cancer Tianjin Medical University Cancer Institute and Hospital National Clinical Research Center for Cancer Key Laboratory of Cancer Prevention and Therapy Tianjin Tianjin’s Clinical Research Center for Cancer Department of Pathology Jinling Hospital Nanjing University School of Medicine State Key Laboratory of Molecular Oncology Department of Medical Oncology National Cancer Center/Canc

Next-generation sequencing(NGS) technology is capable of sequencing millions or billions of DNA molecules ***, it represents a promising tool for the analysis of molecular targets for the initial diagnosis of disease, monitoring of disease progression, and identifying the mechanism of drug resistance. On behalf of the Tumor Biomarker Committee of the Chinese Society of Clinical Oncology(CSCO) and the China Actionable Genome Consortium(CAGC), the present expert group hereby proposes advisory guidelines on clinical applications of NGS technology for the analysis of cancer driver genes for precision cancer therapy. This group comprises an assembly of laboratory cancer geneticists, clinical oncologists, bioinformaticians,pathologists, and other professionals. After multiple rounds of discussions and revisions, the expert group has reached a preliminary consensus on the need of NGS in clinical diagnosis, its regulation, and compliance standards in clinical sample collection. Moreover, it has prepared NGS criteria, the sequencing standard operation procedure(SOP), data analysis, report, and NGS platform certification and validation.

关键词： Next-generation sequencing technology cancer consensus

The first data release(DR1) of the LAMOST regular survey

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Research in Astronomy and Astrophysics 2015年第8期15卷 1095-1124页

作者： A-Li Luo Yong-Heng Zhao Gang Zhao Li-Cai Deng Xiao-Wei Liu yi-Peng jing Gang Wang Hao-Tong Zhang Jian-Rong Shi Xiang-qun Cui Yao-quan Chu Guo-Ping Li zhong-Rui Bai yue Wu Yan Cai Shu-yun Cao Zi-Huang Cao Jeffrey L.Carlin hai-yuan Chen Jian-Jun Chen Kun-Xin Chen Li Chen xue-Lei Chen Xiao-Yan Chen ying Chen Norbert Christlieb Jia-Ru Chu Chen-Zhou Cui yi-Qiao Dong bing Du Dong-Wei Fan Lei Feng Jian-Ning Fu Peng Gao xue-Fei Gong Bo-zhong Gu Yan-Xin Guo Zhan-Wen Han Bo-Liang He jin-Liang Hou Yong-Hui Hou Wen Hou Hong-Zhuan Hu Ning-sheng Hu zhong-Wen Hu Zhi-ying Huo Lei Jia Fang-Hua Jiang Xiang Jiang Zhi-Bo Jiang Ge jin Xiao Kong xu Kong Ya-Juan Lei Ai-Hua Li Chang-Hua Li Guang-Wei Li hai-Ning Li Jian Li Qi Li Shuang Li Sha-Sha Li Xin-Nan Li Yan Li yin-Bi Li Ye-Ping Li yuan Liang Chien-cheng Lin Chao Liu Gen-Rong Liu Guan-qun Liu Zhi-Gang Liu Wen-Zhi Lu yu Luo yin-Dun Mao Heidi Newberg Ji-Jun Ni Zhao-Xiang Qi Yong-Jun Qi Shi-yin shen Huo-Ming Shi jing Song yi-Han Song Ding-Qiang Su Hong-Jun Su Zheng-Hong Tang Qing-sheng Tao yuan Tian Dan Wang Da-Qi Wang Feng-Fei Wang Guo-Min Wang hai Wang Hong-Chi Wang Jian Wang Jia-Ning Wang Jian-Ling Wang Jian-Ping Wang Jun-Xian Wang Lei Wang Meng-Xin Wang Shou-Guan Wang Shu-Qing Wang Xia Wang Ya-Nan Wang You Wang yue-Fei Wang You-Fen Wang Peng Wei Ming-Zhi Wei Hong Wu Ke-Fei Wu xue-bing Wu yu-zhong Wu Xiao-Zheng Xing Ling-Zhe xu Xin-Qi xu Yan xu Tai-sheng Yan De-Hua Yang hai-Feng Yang Hui-Qin Yang Ming Yang Zheng-Qiu Yao Yong yu Hui yuan hai-Bo yuan hai-Long yuan Wei-Min yuan Chao Zhai En-Peng Zhang Hua-Wei Zhang Jian-Nan Zhang Li-Pin Zhang Wei Zhang Yong Zhang Yan-Xia Zhang Zheng-Chao Zhang Ming Zhao Fang Zhou xu Zhou jie Zhu Yong-Tian Zhu Si-cheng Zou Fang Zuo National Astronomical Observatories Chinese Academy of Sciences Department of Astronomy Peking University Shanghai Astronomical Observatory Chinese Academy of Sciences Nanjing Institute of Astronomical Optics & Technology National Astronomical ObservatoriesChinese Academy of Sciences University of Science and Technology of China Department of Physics Applied Physics and AstronomyRensselaer Polytechnic InstituteTroyNY 12180USA Department of Physics and Astronomy Earlham CollegeRichmondIN 47374USA Universitt Heidelberg Zentrum für AstronomieLandessternwarteKnigstuhl 1269117 HeidelbergGermany Department of Astronomy Beijing Normal University yunnan Observatories Chinese Academy of Sciences Purple Mountain Observatory Chinese Academy of Sciences

The Large sky Area Multi-Object Fiber Spectroscopic Telescope（LAMOST） general survey is a spectroscopic survey that will eventually cover approximately half of the celestial sphere and collect 10 million spectra of stars, galaxies and QSOs. Objects in both the pilot survey and the first year regular survey are included in the LAMOST DR1. The pilot survey started in October 2011 and ended in June 2012, and the data have been released to the public as the LAMOST Pilot Data Release in August 2012. The regular survey started in September 2012, and completed its first year of operation in June 2013. The LAMOST DR1 includes a total of 1202 plates containing 2 955 336 spectra, of which 1 790 879 spectra have observed signalto-noise ratio（SNR） ≥ 10. All data with SNR ≥ 2 are formally released as LAMOST DR1 under the LAMOST data policy. This data release contains a total of 2 204 696 spectra, of which 1 944 329 are stellar spectra, 12 082 are galaxy spectra and 5017 are quasars. The DR1 not only includes spectra, but also three stellar catalogs with measured parameters： late A,FGK-type stars with high quality spectra（1 061 918 entries）, A-type stars（100 073 entries）, and M-type stars（121 522 entries）. This paper introduces the survey design, the observational and instrumental limitations, data reduction and analysis, and some caveats. A description of the FITS structure of spectral files and parameter catalogs is also provided.

关键词： techniques: spectroscopic survey—data release—catalog

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柳河盆地中生代充填特征与盆地演化

世界地质 2018年第1期37卷 185-198页

作者：曲鹏展程日辉宋立斌沈艳杰许中杰吉林大学地球科学学院长春130061 吉林油田公司勘探开发研究院吉林松原138000

柳河盆地中生代地层发育有中侏罗统侯家屯组,下白垩统果松组、鹰嘴砬子组、林子头组、下桦皮甸子组和亨通山组。主要岩石类型为碎屑岩、火山碎屑沉积岩、火山碎屑岩和熔岩,沉积相为扇三角洲-湖泊相。根据岩性变化和岩相组合,将下白垩统... 详细信息

柳河盆地中生代地层发育有中侏罗统侯家屯组,下白垩统果松组、鹰嘴砬子组、林子头组、下桦皮甸子组和亨通山组。主要岩石类型为碎屑岩、火山碎屑沉积岩、火山碎屑岩和熔岩,沉积相为扇三角洲-湖泊相。根据岩性变化和岩相组合,将下白垩统划分为13个三级层序和8种充填类型。根据盆地构造和层序特征,划分为5个构造发育阶段,分别是中侏罗世初始凹陷阶段、晚侏罗世抬升剥蚀阶段和早白垩世的3个火山喷发-沉降阶段。柳河盆地是一个受走滑张扭-走滑压扭机制控制的走滑伸展盆地。

关键词：柳河盆地中生代沉积相充填类型构造阶段

林蛙油复方冲剂对X射线辐射大鼠生化指标的影响

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职业与健康 2012年第7期28卷 782-784页

作者：曲保忠吕世杰王新成沈楠徐俊杰吉林医药学院口腔影像系吉林省吉林市132013

目的探讨以长白山特产林蛙油为主要成分的复方冲剂对X线辐射大鼠血清中葡萄糖(BG)、胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇HDL-C)和低密度脂蛋白胆固醇(LDL-C)的影响。方法大鼠复制X射线辐射模型,用林蛙油复方冲剂进行干预,检... 详细信息

目的探讨以长白山特产林蛙油为主要成分的复方冲剂对X线辐射大鼠血清中葡萄糖(BG)、胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇HDL-C)和低密度脂蛋白胆固醇(LDL-C)的影响。方法大鼠复制X射线辐射模型,用林蛙油复方冲剂进行干预,检测各组大鼠血清中BG、TC、TG、HDL-C和LDL-C含量。结果林蛙油复方冲剂可降低TG和LDC-C的浓度,高剂量组能升高HDL-C浓度。结论以林蛙油为主要成分的复方冲剂,对X射线辐射大鼠生化指标有一定的影响。

关键词： X射线辐射林蛙油生化指标

Consensus statement on layout and delivery of automatic external defibrillator in China

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Journal of hainan Medical University 2020年第15期26卷 18-23页

作者： yu Cao Song Chen Yan-Fen Chai Xiao-Hui Chen yu-Guo Chen Shao-Wen cheng Wen-Wei cheng Bang-Han Ding Yang Deng jie Fu Bang-Jiang Fang Mai-ying Fan Li Gui Ping Gong Wei Guo Shu-bin Guo Liang Huang Ji-yuan Han zhong Ji Xian-Fei Ji Xin-Cai Ji Hong-xu jin Xin-Jun Jiang Wang-Xiang Jiang Jia-Liang Liu Xiao-Ran Liu Zhi Liu Chuan-Zhu Lv Feng Lu Tan-Shi Li Xin Li Yong Li Shang-Lun Li Shuang-Ming Li Xiao-Gang Li Bao-Jun Li Pei-Wu Li Chun-sheng Li Zhi-Jun Li Chang-Luo Li Xiang-Min Li yin-Ping Li Zong-Jun Lu Zu-xun Lu zhong-Qiu Lu Zhi-Qian Luo Xiao-Xing Liao Jian-Bo Lei Tao Ma yu Ma yue-Feng Ma Qing-Bian Ma Lin-Hao Ma Jun-Qi Ma Shi-Nan Nie jie-Miao Ou-Yang Peng Peng Qiao Pei Shu-Ming Pan Guo-zhong Qing Li-jie Qin Wu-ying Qiao Fei Shao Wei Song Xing-yue Song Zhen-Ju Song Rong-Ju Sun De-Ya Shang Du-Juan Sha Gui-Lan Tao Guo-Gang Tian ying-Ping Tian Jian-Guang Tian Chao-Yang Tong Zi-Ren Tang Meng-Xiong Tang Guo-Ping Wu Zhi Wan Tao Wang Xin Wang Peng Wang zhong Wang Tong Wang Ri-Xing Wang yi-Xin Wang Xiao-Dan Wang Ling Wu Wei Wei Hua Xiang Feng xu Jun xu jie xu Miao-Rong Xie Shuo-Gui xu Tie xu Feng yu Wen yi Li-Shan Yang Shi-Jiao Yan jin-Jian Yao Shou-Chun Yan xue-zhong yu Xian-Zhi Ye Xian-Liang Yan Fu-bing Zhou Rong-bin Zhou Ming Zhou Qiang Zhou jin Zhou Ping Zhou Chang-Ju Zhu Hua-Dong Zhu Ji-Hong Zhu Hong Zhang Mao Zhang Hua Zhang Na Zhang Guo-Qiang Zhang Guo-Xiu Zhang jin-Song Zhang Wen-Wu Zhang Wen-zhong Zhang Jian-Feng Zhang yi-Min Zhu Jun Zeng yuan Zheng Zhi-jie Zheng shen Zhao Yan Zhao Min Zhao bin Zhao Xiao-Dong Zhao Xiao-jing Zhao Hong-Ke Zeng sheng-Qiang Zou The Chinese Society of Emergency Medicine Research Unit of Island Emergency Medicine Chinese Academy of Medical SciencesHainan Medical University Key Laboratory of Emergency and Trauma of Ministry of Education Hainan Medical UniversityHaikouHainanChina Emergency and Trauma College Hainan Medical UniversityHaikouHainanChina Department of Emergency Hainan Clinical Research Center for Acute and Critical DiseasesThe Second Affiliated Hospital of Hainan Medical UniversityHaikouHainanChina Key Laboratory of hainan Trauma and Disaster Rescue Key Laboratory of Trauma of hainan Medical University

*** Sudden cardiac death(SCD)refers to sudden death due to a variety of cardiac *** is manifested as a sudden loss of consciousness,cardiac arrest,and respiratory arrest and generally shows early symptoms that are atypical and not easily detected[1].According to a recent study,nearly 544000 patients suffer from SCD every year in China[2].

关键词： cardiac death respiratory