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New phenylpyridone derivatives from the Penicillium sumatrense GZWMJZ-313,a fungal endophyte of Garcinia multiflora

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Chinese Chemical Letters 2019年第2期30卷 431-434页

作者： yanchao xu Liping Wang Guoliang Zhu Mingxing Zuo Qianyu Gong Wenwen He Mingpeng Li Chunmao Yuan Xiaojiang Hao Weiming Zhu State Key Laboratory of Functions and Applications of Medicinal Plants Guizhou Medical University School of Pharmacuetical Sciences Guizhou Medical University Key Laboratory of Chemistry for Natural Products of Guizhou Province Chinese Academy of Sciences Key Laboratory of Marine Drugs Ministry of Education of China School of Medicine and Pharmacy Ocean University of China

Citridones E-G (1-3), three new phenylpyridone derivatives together with two known curvularins (4 and 5) were isolated from the solid culture of the endophytic fungus Penicillium sumatrense GZWMJZ-313 in Garcinia multiflora. The structures of new compounds were determined in the light of spectroscopic data,X-ray and ECD calculation. Compounds 1 and 3 are racemates, while compound 2 is optically *** 1 and 4 showed antibacterial and antifungal activities against Staphylococcus aureus,Pseudomonas aeruginosa, Clostridium perfringens, Escherichia coli and Candida albicans with MIC values ranging from 8 μg/mL to 64 μg/mL.

关键词： Penicillium sumatrense Garcinia multiflora Pyridones Antibacterial activities Endophytic fungi

Copper-linked IT MoS2/Cu2O Heterostructure for Efficient Photocatalytic Hydrogen Evolution

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Chemical Research in Chinese Universities 2020年第6期36卷 1122-1127页

作者： YIN Yage WEI Shuting ZHANG Lei GUO Ziwang HUANG Haihua SAl Shiran WU Jiandong xu yanchao LIU Ying ZHENG Lirong FAN Xiaofeng CUI Xiaogiang Key Laboratory of Automobile Materials of Ministry of Education and School of Materials Science and Engineering Jilin UniversityChangchun/30012.P R.China College of Chemistry Jilin UniversityChangchun 130012P.R.China Beijing Synchrotron Radiation Facility.Institute of High Energy Physics Chinese Academy of SciencesBeijing 100049.P.R.China

Metallic IT phase molybdenum disulfide(lT MoS2)is an excellent catalyst due to the abundant active sites and good ***,complex synthesis and unstable nature of IT MoS2 still limit its practical ***,we propose a simple method to trigger the phase transition of *** phase transition is first predicted by density functional theory(DFT)calculations and then confirmed by Raman spectroscopy and high-resolution transmission electron microscopy(HRTEM).IT MoS2/CU2O heterostructure catalyst is then developed,showing enhanced photocatalytic hydrogen evolution.

关键词： Photocatalysis Hydrogen evolution Phase transition Heterostructure

Effects of dietary macronutrients and body composition on glucose homeostasis in mice

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National Science Review 2021年第1期8卷 19-29页

作者： Sumei Hu Jacques Togo Lu Wang Yingga Wu Dengbao Yang yanchao xu Li Li Baoguo Li Min Li Jianbo Li Guanlin Wang xueying Zhang Chaoqun Niu Mohsen Mazidi Alex Douglas John R.Speakman State Key Laboratory of Molecular Developmental Biology Institute of Genetics and Developmental Biology Chinese Academy of Sciences Institute of Biological and Environmental Sciences University of Aberdeen Key Laboratory of Molecular Pharmacology and Drug Evaluation (Ministry of Education of China) School of Pharmacy Yantai University School of Basic Medical Sciences University of Dali University of Chinese Academy of Sciences CAS Center for Excellence in Animal Evolution and Genetics (CCEAEG)

As a major health issue, obesity is linked with elevated risk of type 2 diabetes. However, whether disrupted glucose homeostasis is due to altered body composition alone, or whether dietary macronutrients play an additional role, independent of their impact on body composition, remains unclear. We investigated the associations between macronutrients, body composition, blood hormones and glucose homeostasis. We fed C57BL/6N mice 29 different diets with variable macronutrients for 12 weeks. After 10 weeks,intraperitoneal glucose tolerance tests were performed. Generalized linear models were generated to evaluate the impacts of macronutrients, body composition and blood hormones on glucose *** area under the glucose curve (AUC) was strongly associated with body fat mass, but not dietary macronutrients. AUC was significantly associated with fasting insulin levels. Six genes from transcriptomic analysis of epididymal white adipose tissue and subcutaneous white adipose tissue were significantly associated with AUC. These genes may encode secreted proteins that play important previously unanticipated roles in glucose homeostasis.

关键词： dietary macronutrients body fat mass fasting blood glucose glucose tolerance mice

The morphological diversity of pollen in the genus Gossypium

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Journal of Cotton Research 2023年第1期6卷 48-59页

作者： CAI Xiaoyan HOU Yuqing MUHAMMAD Umer Jawad WANG Heng xu yanchao ZHENG Jie WANG Yuhong LIU Fang ZHOU Zhongli HUA Jinping WANG Kunbo State Key Laboratory of Cotton Biology Institute of Cotton ResearchChinese Academy of Agricultural SciencesAnyang 455000HenanChina National Nanfan Research Institute(Sanya) Chinese Academy of Agriculture SciencesSanya 572025HainanChina College of Agronomy and Biotechnology China Agricultural UniversityBeijing 100193China

Background Plant pollen has diverse morphological characteristics that can be consistently passed down from generation to *** on pollen morphology is thus immensely important for plant classification and *** the genus Gossypium,however,in-depth research on pollen morphology is lacking,with only few reports on limited cotton *** evaluate the diversity of pollen in Gossypium,we therefore conducted a comprehensive analysis of the pollen morphology of 33 cotton species and varieties using scanning electron *** The 33 analyzed cotton samples exhibited common pollen morphological features,including spherical shapes,radial symmetry,echination,panporation,and operculation,while the pollen size,spine shape,spine density and length showed distinctive *** size varied significantly among species,with diameters ranging from62.43 μm in *** to 103.41 μm in *** exine had an echinate sculptural texture,and spines were mostly conical or sharply conical but occasionally *** density varied from 173 in *** to 54 in ***,while spine length ranged from 3.53 μm in *** to 9.47 μm in *** addition,the 33cotton species and varieties were grouped at a genetic distance of 3.83 into three *** Ⅰ comprised five allotetraploid AD-genome cotton species,four D-genome species,and one K-genome *** Ⅱ included 13diploid species from A,B,D,E,and G genomes,whereas Cluster Ⅲ only consisted one E-genome species *** Although pollen characteristics alone are not enough to resolve taxonomic and systematic relationships within the genus Gossypium,our results add to knowledge on palynomorphology and contribute to phenological information on these *** findings should aid future systematic and phylogenetic studies of the Gossypium genus.

关键词： Cotton Scanning electron microscopy Pollen morphology Diversity

Genome-wide identification of OSCA gene family and their potential function in the regulation of dehydration and salt stress in Gossypium hirsutum

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Journal of Cotton Research 2019年第2期2卷 87-104页

作者： YANG Xiu xu yanchao YANG Fangfang MAGWANGA Richard Odongo CAI Xiaoyan WANG Xingxing WANG Yuhong HOU Yuqing WANG Kunbo LIU Fang ZHOU Zhongli Institute of Cotton Research Chinese Academy of Agricultural Science (ICRCAAS)/Research Base in Anyang Institute of TechnologyState Key Laboratory of Cotton Biology

Background:Cotton(Gossypium hirsutum) provides the largest natural fiber for the textile manufacturing industries,but its production is on the decline due to the effects of *** salt-alkalization leads to damage in cotton growth and a decrease in ***-gated calcium-permeable channels(OSCA) have been found to be involved in the detection of extracellular changes which trigger an increase in cytosolic free calcium ***-induced calcium ion increases have been widely speculated to be playing a role in osmosensing in ***,the molecular nature of the corresponding calcium ion channels remains *** this research work,we describe the OSCA genes and their putative function in osmosensing in plants by carrying out genomewide identification,characterization and functional analysis of the significantly up-regulated OSCA gene,GhOSCA1.1 through reverse ***:A total of 35,21 and 22 OSCA genes were identified in ***,***,and *** genomes,respectively,and were classified into four different clades according to their gene structure and phylogenetic *** and protein structure analysis indicated that 35 GhOSCA genes contained a conserved RSN17 TM(PF02714) ***,the cis-regulatory element analysis indicated that the OSCA genes were involved in response to abiotic ***,the knockdown of one of the highly up-regulated genes,GhOSCA1.1 showed that the virus-induced gene silenced(VIGS) plants were highly sensitive to dehydration and salinity stresses compared with the none VIGS plants as evident with higher concentration levels of oxidant enzymes compared with the antioxidant enzymes on the leaves of the stressed ***:This study provides the first systematic analysis of the OSCA gene family and will be important for understanding the putative functions of the proteins encoded by the OSCA genes in *** results provide a new insight of de

关键词： OSCA gene family Gossypium hirsurum VIGS Salt and dehydration stress

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庙沟铁矿东排土场稳定性研究及治理措施探讨

金属矿山 2011年第11期40卷 48-52页

作者：许雁超河北钢铁集团矿业有限公司庙沟铁矿

通过总结庙沟铁矿矿山开采现状及排土场现状,提出由于岩土堆存带来的地质环境问题。从地形地貌、地层岩性组成及岩石物理力学性质等4个方面分析该区工程地质特征,同时阐述区内水文地质概况。在采用钻探、原位测试进行野外地质观察和分... 详细信息

通过总结庙沟铁矿矿山开采现状及排土场现状,提出由于岩土堆存带来的地质环境问题。从地形地貌、地层岩性组成及岩石物理力学性质等4个方面分析该区工程地质特征,同时阐述区内水文地质概况。在采用钻探、原位测试进行野外地质观察和分析该区周边岩体节理特征的基础上,确定排土场稳定性计算剖面并选取计算参数,通过进行东排土场稳定性计算,提出东排土场治理具体措施,并从社会、环境和经济等3个方面进行排土场治理综合效益预测。

关键词：排土场稳定性治理

Widespread incomplete lineage sorting and introgression shaped adaptive radiation in the Gossypium genus

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Plant Communications 2024年第2期5卷 185-197页

作者： yanchao xu Yangyang Wei Zhongli Zhou Xiaoyan Cai Scott ABoden Muhammad Jawad Umer Luqman BSafdar Yuling Liu Dingsha Jin Yuqing Hou Yuhong Wang Sarah Brooke Wall Kunbo Wang Shuxun Yu Baohong Zhang Renhai Peng Fang Liu National Key Laboratory of Cotton Bio-breeding and Integrated Utilization Institute of Cotton ResearchChinese Academy of Agricultural SciencesAnyang 455000China College of Plant Science and Technology Huazhong Agricultural UniversityWuhan 40070China College of Biology and Food Engineering Anyang Institute of TechnologyAnyang 455000China National Nanfan Research Institute(Sanya) Chinese Academy of Agricultural SciencesSanya 572024China School of Agriculture Food and WineUniversity of AdelaideAdelaideSA 5005Australia Sanya Institute Hainan Academy of Agricultural SciencesSanya 572000China Department of Biology East Carolina UniversityGreenvilleNC 27858USA School of Agricultural Sciences Zhengzhou UniversityZhengzhou 450001China

Cotton(Gossypium)stands as a crucial economic crop,serving as the primary source of naturalfiber for the textile ***,the evolutionary mechanisms driving speciation within the Gossypium genus remain *** this investigation,we leveraged 25 Gossypium genomes and introduced four novel assem-blies—***,***,***,and ***(Gklo)—to delve into the speciation history of this ***,we encountered intricate phylogenies potentially stemming from *** complexities are further compounded by incomplete lineage sorting(ILS),a factor likely to have been instrumental in shaping the swift diversification of *** focus subsequently shifted to the rapid radiation episode during a concise period in Gossypium *** a recently diverged lineage comprising ***,Gklo,and ***,we constructed afinely detailed ILS ***,this analysis revealed the non-random distribution of ILS regions across the reference Gklo ***,we identified signs of robust natural selection influencing specific ILS *** variations per-taining to speciation emerged between the closely related sister species Gklo and ***-mately 15.74%of speciation structural variation genes and 12.04%of speciation-associated genes were esti-mated to intersect with ILS *** enrich our understanding of the role of ILS in adaptive radiation,shedding fresh light on the intricate speciation history of the Gossypium genus.

关键词： cotton speciation Gossypium genus incomplete lineage sorting ILS phylogenetic analysis gene tree resolution

Knockdown of 60S ribosomal protein L14‑2 reveals their potential regulatory roles to enhance drought and salt tolerance in cotton

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Journal of Cotton Research 2021年第4期4卷 308-321页

作者： SHIRAKU Margaret Linyerera MAGWANGA Richard Odongo CAI Xiaoyan KIRUNGU Joy Nyangasi xu yanchao MEHARI Teame Gereziher HOU Yuqing WANG Yuhong WANG Kunbo PENG Renhai ZHOU Zhongli LIU Fang State Key Laboratory of Cotton Biology/Institute of Cotton Research Chinese Academy of Agricultural Science(ICRCAAS)Anyang 455000HenanChina School of Biological and Physical Sciences(SBPS) Main CampusJaramogi Oginga Odinga University of Science and Technology(JOOUST)Main CampusP.O.Box 210‑40601BondoKenya Anyang Institute of Technology Anyang 455000HenanChina School of Agricultural Sciences Zhengzhou UniversityZhengzhou 450001HenanChina

Background:Cotton is a valuable economic crop and the main significant source of natural fiber for textile industries *** effects of drought and salt stress pose a challenge to strong fiber and large-scale production due to the ever-changing climatic ***,plants have evolved a number of survival strategies,among them is the induction of various stress-responsive genes such as the ribosomal protein large(RPL)*** RPL gene families encode critical proteins,which alleviate the effects of drought and salt stress in *** this study,comprehensive and functional analysis of the cotton RPL genes was carried out under drought and salt ***:Based on the genome-wide evaluation,26,8,and 5 proteins containing the RPL14B domain were identified in Gossypium hirsutum,***,and ***,***,through bioinformatics analysis,key cis-regulatory elements related to RPL14B genes were *** Myb binding sites(MBS),abscisic acid-responsive element(ABRE),CAAT-box,TATA box,TGACG-motif,and CGTCA-motif responsive to methyl jasmonate,as well as the TCA-motif responsive to salicylic acid,were *** analysis revealed a key gene,Gh_D01G0234(RPL14B),with significantly higher induction levels was further evaluated through a reverse genetic *** knockdown of Gh_D01G0234(RPL14B)significantly affected the performance of cotton seedlings under drought/salt stress conditions,as evidenced by a substantial reduction in various morphological and physiological ***,the level of the antioxidant enzyme was significantly reduced in VIGS-plants,while oxidant enzyme levels increased significantly,as demonstrated by the higher malondialdehyde concentration ***:The results revealed the potential role of the RPL14B gene in promoting the induction of antioxidant enzymes,which are key in oxidizing the various *** key pathways need to be investigated and even as we exploit these genes

关键词： Abiotic stress Cotton Ribosomal protein large Transcription factor Virus-induced gene silencing

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Genome-wide identification and analysis of the CNGC gene family in upland cotton under multiple stress conditions

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Journal of Cotton Research 2023年第3期6卷 195-208页

作者： KIRUNGU Joy Nyangasi MAGWANGA Richard Odongo SHIRAKU Margaret Linyerera OKUTO Erick CAI Xiaoyan xu yanchao HOU Yuqing AGONG' Stephen Gaya WANG Kunbo WANG Yuhong ZHOU Zhongli LIU Fang State Key Laboratory of Cotton Biology/Institute of Cotton Research Chinese Academy of Agricultural Science(ICR-CAAS)AnyangHenan 455000China School of Biological PhysicalMathematics and Actuarial Sciences(SBPMAS)Jaramogi Oginga Odinga University of Science and Technology(JOOUST)Bondo210‑40601Kenya School of Agricultural Sciences Zhengzhou UniversityZhengzhouHenan 450001China

Background The cyclic nucleotide-gated channel(CNGC)gene family plays a significant role in the uptake of both essential and toxic cations,and has a role in enhancing tolerance to various forms of abiotic stresses as well as the modulation of the heavy metal toxicity to plant through the absorption of heavy *** A complete genome-wide identification and functional characterization of the cotton CNGC genes was carried out,in which 55,28,and 29 CNGC genes were identified in Gossypium hirsutum,***,and ***,*** protein encoded by the CNGC genes exhibited GRAVY value below zero,indicating their hydrophilic *** genes were unevenly distributed in 19 out of 26 chromosomes,in which the highest density were observed on Ah05,with 8 *** gene coverage was observed among the diploid cotton species,with CNGC genes mapped on all A chromosomes and on 11 out of 13 of D *** majority of CNGC proteins were localized in the endoplasmic reticulum,nucleus,and plasma *** expression analysis revealed the up-regulation of Gh_A01G0520(CNGC4)and Gh_D13G1974(CNGC5)across various forms of abiotic ***,down-regulation of Gh_A01G0520(CNGC4)and Gh_D13G1974(CNGC5)in CNGCs silenced plants caused the significantly reduced ability to tolerate drought and salt *** CNGCs silenced plants were recorded to have significantly low content of antioxidants but relatively higher content of oxidant,including MDA and H_(2)O_(2).Furthermore,SPAD,CMS(cell membrane stability),ELWL(excised leaf water loss),SDW(shoot dry matter weight),and RDW(root dry matter weight)were all lower in CNGCs silenced plants compared with the wild type *** Significant reduction in antioxidant content and negative effects of physiological and morphological characters in CNGCs silenced plants has revealed the novel role of CNGC genes in enhancing cell integrity under abiotic stress *** results provide vital information

关键词： Cyclic nucleotide-gated channel Abiotic stress Differential expression VIGS-plants Chromosome Tetraploid cotton

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