Genome-wide identification and analysis of the CNGC gene family in upland cotton under multiple stress conditions

作     者：KIRUNGU Joy Nyangasi MAGWANGA Richard Odongo SHIRAKU Margaret Linyerera OKUTO Erick CAI Xiaoyan XU Yanchao HOU Yuqing AGONG' Stephen Gaya WANG Kunbo WANG Yuhong ZHOU Zhongli LIU Fang KIRUNGU Joy Nyangasi;MAGWANGA Richard Odongo;SHIRAKU Margaret Linyerera;OKUTO Erick;CAI Xiaoyan;XU Yanchao;HOU Yuqing;AGONG'Stephen Gaya;WANG Kunbo;WANG Yuhong;ZHOU Zhongli;LIU Fang

作者机构：State Key Laboratory of Cotton Biology/Institute of Cotton ResearchChinese Academy of Agricultural Science(ICR-CAAS)AnyangHenan 455000China School of BiologicalPhysicalMathematics and Actuarial Sciences(SBPMAS)Jaramogi Oginga Odinga University of Science and Technology(JOOUST)Bondo210‑40601Kenya School of Agricultural SciencesZhengzhou UniversityZhengzhouHenan 450001China 

出 版 物：《棉花研究(英文)》 (Journal of Cotton Research)

年 卷 期：2023年第6卷第3期

页      面：195-208页

学科分类：09[农学] 0901[农学-作物学] 

基　　金：funded by the National Natural Science Foundation of China(31621005,32072023) National Key R&D Program of China(2021YFE0101200) PSF/CRP/18th Protocol(07) 

主　　题：Cyclic nucleotide-gated channel Abiotic stress Differential expression VIGS-plants Chromosome Tetraploid cotton 

摘      要：Background The cyclic nucleotide-gated channel(CNGC)gene family plays a significant role in the uptake of both essential and toxic cations,and has a role in enhancing tolerance to various forms of abiotic stresses as well as the modulation of the heavy metal toxicity to plant through the absorption of heavy *** A complete genome-wide identification and functional characterization of the cotton CNGC genes was carried out,in which 55,28,and 29 CNGC genes were identified in Gossypium hirsutum,***,and ***,*** protein encoded by the CNGC genes exhibited GRAVY value below zero,indicating their hydrophilic *** genes were unevenly distributed in 19 out of 26 chromosomes,in which the highest density were observed on Ah05,with 8 *** gene coverage was observed among the diploid cotton species,with CNGC genes mapped on all A chromosomes and on 11 out of 13 of D *** majority of CNGC proteins were localized in the endoplasmic reticulum,nucleus,and plasma *** expression analysis revealed the up-regulation of Gh_A01G0520(CNGC4)and Gh_D13G1974(CNGC5)across various forms of abiotic ***,down-regulation of Gh_A01G0520(CNGC4)and Gh_D13G1974(CNGC5)in CNGCs silenced plants caused the significantly reduced ability to tolerate drought and salt *** CNGCs silenced plants were recorded to have significantly low content of antioxidants but relatively higher content of oxidant,including MDA and H_(2)O_(2).Furthermore,SPAD,CMS(cell membrane stability),ELWL(excised leaf water loss),SDW(shoot dry matter weight),and RDW(root dry matter weight)were all lower in CNGCs silenced plants compared with the wild type *** Significant reduction in antioxidant content and negative effects of physiological and morphological characters in CNGCs silenced plants has revealed the novel role of CNGC genes in enhancing cell integrity under abiotic stress *** results provide vital information